Study of the effect of Ginkgo biloba and quercetin extracts on the mouse vas deferens
DOI:
https://doi.org/10.33448/rsd-v11i12.33441Keywords:
Herbal medicines; Premature ejaculation; Ginkgo biloba; Quercetin; Vas deferens.Abstract
Ginkgo biloba extract (GBE) has antioxidant, anti-inflammatory, and neuroprotective actions, and has a relaxing effect on smooth muscle organs. The vas deferens (DD) conducts the spermatozoa during ejaculation by the contraction of its muscles, and changes in this contraction can lead to disorders such as premature ejaculation (PE). PE is ejaculation that always or almost always occurs before or about 1 minute after vaginal penetration. Thus, our aim was to study the effect of GBE under the contraction of the DD of mice. The effect of GBE, here called GB2, or quercetin, was tested against contraction with potassium chloride. The effect of these substances was tested in the presence of nifedipine. We demonstrate that GB2, quercetin and nifedipine are able to inhibit the phasic and tonic components of the contraction. The effect of GB2 on the tonic component was enhanced in the presence of quercetin. Comparing the tonic component between GB2, Nifedipine+GB2 and Quercetin+GB2 groups, we observed that in the presence of nifedipine or quercetin, the effect of GB2 was potentiated. In the comparison of the Quercetin and Nifedipine+Quercetin groups, nifedipine potentiated the effect of quercetin under both components. These results indicate that GB2 and quercetin are able to inhibit some mechanism involved with the generation of DD contraction, and that this effect is not solely dependent on voltage-dependent calcium channel inhibition. These findings point out that GBE and quercetin, may be pharmacological strategies for the treatment of PE.
References
Amin, A., Abraham, C., Hamza, A. A., Abdalla, Z. A., Al-shamsi, S. B., Harethi, S. S., & Daoud, S. (2012). A standardized extract of Ginkgo biloba neutralizes cisplatin-mediated reproductive toxicity in rats. J Biomed Biotechnol. 2012:362049.
Balanz, L., & Leon, M. (1994). Evidence of an oxidative challenge in the Alzheimer’s brain. Neurochem Res. 19(9): 1131–7.
Brasil. Farmacopeia Brasileira. (2010). Agência Nacional de Vigilância Sanitária. Brasília: Anvisa. (5ª edição.) v. 10.
Brasil (2006a). Ministério da Saúde Portaria no 971, de 03 de maio de 2006. Diário Oficial da União, Brasília (DF).
Brasil. (2016). Ministério da Saúde. Política e Programa Nacional de Plantas Medicinais e Fitoterápicos. Diário Oficial da União, Brasília (DF).
Brasil. (2018). Ministério da Saúde. Saúde Sexual e Saúde Reprodutiva: os homens como sujeitos de cuidado. Diário Oficial da União, Brasília (DF).
Brasil. (2006b). Ministério da Saúde. Decreto nº 5.813. Aprova Política Nacional de Plantas Medicinais e Fitoterápicos. Diário Oficial da União, Brasília (DF)
Bruschini, H., Schmidt, R. A., & Tanagho, E. A. (1977). Studies on the neurophysiology of the vas deferens. Investigative Urology. 15(2):112-6.
Capasso, R., Fiorino, F., Ascione, V., Frecentese, F., & Borrelli, F. (2006). Inhibition of rat vas deferens contractions by flavonoids in-vitro. J Pharm Pharmacol. 58(3):381-4.
Catterall, W. A., Lenaeus, M. J., & El-din, T. M. G. (2020). Structure and Pharmacology of Voltage-Gated Sodium and Calcium Channels. Annu Rev Pharmacol Toxicol. 6;60:133-154.
Catterall, W. A., Perez-Reyes, E., Snutch, T. P., & Striessnig, J. (2005). International Union of Pharmacology. XLVIII. Nomenclature and structure-function relationships of voltage-gated calcium channels. Pharmacological reviews, 57(4), 411–425.
Chen, X., Salwinski, S., & Lee, T.J. (1997). Extracts of Ginkgo biloba and ginsenosides exert cerebral vasorelaxation via a nitric oxide pathway. Clin Exp Pharmacol Physiol. 24(12):958-9.
Cheng, J. T. (1976) Calcium-induced release of calcium in rectal smooth muscle of mice. Jap. J. Pharmac. 26, 73-78.
Delaflotte, S., Auguet, M., Defeudis, F. V., Baranes, J., Clostre, F., Drieu, K., & Braquet, P. (1984). Endothelium-dependent relaxations of rabbit isolated aorta produced by carbachol and by Ginkgo biloba extract. Biomed Biochim Acta . 43: S212 - S216.
Dell'agli, M., Galli, G. V., & Bosisio, E. (2006). Inhibition of cGMP-phosphodiesterase-5 by biflavones of Ginkgo biloba. Planta Med. 72:468–70.
Del-Vechio-Vieira, G., Santos, B. C. S., Alves, M. S., Araújo, A. L. A., Yamamoto, C. H., Pinto, M. A. O., Kaplan, M. A. C., & Sousa, O. V. (2016). Bioactivities of the ethanol extract from Ageratum fastigiatum branches: antioxidant, antinociceptive and anti-inflammatory. An. Acad. Bras. Ciênc. vol.88 no.3.
Duarte, J. A. R. (1993). Lesões Celulares do Músculo Esquelético Induzidas pelo Exercício Físico. Dissertação de doutorado, Faculdade de Ciências do Desporto e de Educação Física, Porto, Portugal.
Fernandes, I., Pérez-Gregorio, R., Soares, S., Mateus, N., & Freitas, V. (2017). Wine Flavonoids in Health and Disease Prevention. Molecules. 14;22(2):292.
Francischi, F. B., Ayres, D. C., Itao, R. E., Spessoto, L. C. F., Arruda, J. G. F., & Facio, F. N. (2011). Ejaculação precoce: existe terapia eficiente?. Einstein (São Paulo), 9(4), 545-549.
Gachowska, M., Szlasa, W., Saczko, J., & Kulbacka, J. (2021). Neuroregulatory role of ginkgolides. Mol Biol Rep. 48(7):5689-5697.
Garcia-Pascual, O. A., Costa G., Isla, M., Jimenez, E., & Garcia-Sacristan, A. (1991). Potassium-induced contraction in the lamb proximal urethra: involvement of norepinephrine and different calcium entry pathways, The Journal of pharmacology and experimental therapeutics, 256(1), 127–134.
Gavrilova, S. I., Preuss, U. W., Wong, J. W., Hoerr, R., Kaschel, R., & Bachinskaya, N. (2014). Efficacy and safety of Ginkgo biloba extract EGb 761 in mild cognitive impairment with neuropsychiatric symptoms: a randomized, placebo-controlled, double-blind, multi-center trial. Int J Geriatr Psychiatry. 29(10):1087-95.
Gillman, N., & Gillman, M. (2019). Premature Ejaculation: Aetiology and Treatment Strategies. Med Sci (Basel). 25;7(11):102.
Giuliano, F. (2011). Neurophysiology of Erection and Ejaculation. The Journal of Sexual Medicine. 8,310-315.
Guo H., Zhang, J., Gao, W., Qu, Z., & Liu, C. (2014). Anti-diarrhoeal activity of methanol extract of Santalum album L. in mice and gastrointestinal effect on the contraction CROWof isolated jejunum in rats. Journal of Ethnopharmacology. 154(3), 704–710.
Hay, D. W. P., & Wadsworth, R. M. (1982a). Effects of some organic calcium antagonists and other procedures affecting Ca2+ translocation on KCl‐induced contractions in the rat vas deferens. British Journal of Pharmacology. 76; 1.103–113.
Hay, D. W. P., & Wadsworth, R. M. (1982b). KCl contractions in the rat intact and bisected vas deferens: contribution of endogenous noradrenaline release. Clinical and experimental pharmacology & physiology, 10(1), 77–86.
Hoy, W., Ashley, W., & Cameron, A. N. (1986). Effect of a Smooth Muscle Relaxant from the Stonefish, Synanceia Trachynis, on KCl-induced Responses in the Guinea-pig Vas Deferens. European Journal of Pharmacology. 129.1.105-12.
Huddart, H., Langton, P. D., & Saad, K. H. (1984). Inhibition by papaverine of calcium movements and tension in the smooth muscles of rat vas deferens and urinary bladder. The Journal of Physiology. 349:1.183–194.
Jurkiewicz, A., Lafayette, S. S., Nunes, S. H., Martini, L. C., Garcez-do-Carmo, L., & Wanderley, A. (1994). Decreased density of binding sites for the Ca2+ channel antagonist [3H]isradipine after denervation of rat vas deferens. Eur J Pharmacol. 256:329–333.
Kierszenbaum, A. L. (2008). Histologia e biologia celular: uma introdução à patologia. (2° edição): Elsevier.
Kiguti, L. R. A., & Pupo, A. S. (2012). Investigation of the Effects of a1-Adrenoceptor Antagonism and L-Type Calcium Channel Blockade on Ejaculation and Vas Deferens and Seminal Vesicle Contractility In Vitro. Journal of Sexual Medicine. 9:1.159-168.
Kim, J. J., Han, D. H., Lim, S. H., Kim, T. H., Chae, M. R., Chung, K. J., Kam, S. C., Jeon, J. H., Park, J. K., & Lee, S. W. (2011). Effects of Ginkgo biloba extracts with mirodenafil on the relaxation of corpus cavernosal smooth muscle and the potassium channel activity of corporal smooth muscle cells. Asian J Androl. 13(5):742-6.
Kubota, Y., Tanaka, N., Umegaki, K., Takenaka, H., Mizuno, H., Nakamura, K., Shinozuka, K., & Kunitomo, M. (2001). Ginkgo biloba extract-induced relaxation of rat aorta is associated with increase in endothelial intracellular calcium level. Life Sci. 5;69(20):2327-36.
Lafayette, S. S., Vladimirova, I., Garcez-do-carmo, L., Monteforte, P. T., Caricati neto, A., & Jurkiewicz, A. (2008). Evidence for the participation of calcium in non-genomic relaxations induced by androgenic steroids in rat vas deferens. British journal of pharmacology, 153(6), 1242–1250.
Langton, P. D., & Huddart, H. (1987) The involvement of fast calcium channel activity in the selective activation of phasic contractions by partial depolarization in rat vas deferens smooth muscle. Gen. Pharmac. 18, 47-55.
Langton, P. D., & Huddart, H. (1988). Voltage and time dependency of calcium mediated phasic and tonic responses in rat vas deferens smooth muscle—The effect of some calcium agonist and antagonist agents. General pharmacology, 19(6), 775–787.
Lipskaia, L., Limon, I., Bobe, R., & Hajjar, R. (2012). Calcium cycling in synthetic and contractile phasic or tonic vascular smooth muscle cells. In: Current Basic and Pathological Approaches to the Function of Muscle Cells and Tissues - From Molecules to Humans. Intech Publishers. 2: 28–44.
Luo, X., Xue, L., Xu, H., Zhao, Q. Y., Wang, Q., She, Y. S., Zang, D. A., Shen, J., Peng, Y. B., Zhao, P., Yu, M. F., Chen, W., Ma, L. Q., Chen, S., Chen, S., Fu, X., Hu, S., Nie, X., Shen, C., Zou, C., & Liu, Q. H. (2018). Polygonum aviculare L. extract and quercetin attenuate contraction in airway smooth muscle. Scientific reports, 8(1), 3114.
Luz, M. T. (1997). Cultura contemporânea e medicinas alternativas: novos paradigmas em saúde no fim do século XX. Physis. 7(1):13-43
Mattos, G., Camargo, A., Sousa, C. A., & Zeni, A. L. B. (2018). Plantas medicinais e fitoterápicos na Atenção Primária em Saúde: percepção dos profissionais. Ciência & Saúde Coletiva, Rio de Janeiro, 23(11), 3735-3744.
Mcmahon, C. G., Althof, S. E., Waldinger, M. D., Porst, H., Dean, J., Sharlip, I. D., Adaikan, P. G., Becher, E., Broderick, G. A., Buvat, J., Dabees, K., Giraldi, A., Giuliano, F., Hellstrom, W. J., Incrocci, L., Laan, E., Meuleman, E., Perelman, M. A., Rosen, R. C., Rowland, D. L., & Segraves, R. (2008). An evidence-based definition of lifelong premature ejaculation: Report of the International Society for Sexual Medicine (ISSM) ad hoc committee for the definition of premature ejaculation. J Sex Med. 5:1590–606.
Modzelewska, B., Drygalski, K., Kleszczewski, T., Chomentowski, A., Koryciński, K., Kiełczewska, A., Pawłuszewicz, P., & Razak, H. (2021). Quercetin relaxes human gastric smooth muscles directly through ATP-sensitive potassium channels and not depending on the nitric oxide pathway. Neurogastroenterol Motil. 33(7):e14093.
Moynihan, A. T., Smith, T. J., & Morrison, J. J. (2008). The relaxant effect of nifedipine in human uterine smooth muscle and the BKCa channel. American Journal of Obstetrics and Gynecology, 198(2), 237.e1–237.e2378
Nunez, D., Chignard, M., Korth, R., Le couedic, J. P., Norel, X., Spinnewyn, B., Braquet, P., & Benveniste, J. (1986). Specific inhibition of PAF-acether-induced platelet activation by BN 52021 and comparison with the PAF-acether inhibitors kadsurenone and CV 3988. Eur J Pharmacol. 16;123(2):197-205.
Picarelli, Z. P., Hyppolito, N., & Valle, J. R. (1962). Synergistic effect of 5-hydroxytryptamine on the response of rats’s seminal vesicles to adrenaline and noradrenaline. Archives internationales de pharmacodynamie et de therapie, 138, 354–363.
Rivera, J. O., Hughes, H. W., & Stuart, A. G. (2004). Herbals and asthma: usage patterns among a border population. Ann Pharmacother. 38: 220–225.
Rotondo, A., Serio, R., & Mulè, F. (2009). Gastric relaxation induced by apigenin and quercetin: Analysis of the mechanism of action. Life Sciences, 85(1-2), 85–90.
Sakanashi, Y., Oyama, K., Matsui, H., Oyama, T. B., Oyama, T. M., Nishimura, Y., Sakai, H., & Oyama, Y. (2008). Possible use of quercetin, an antioxidant, for protection of cells suffering from overload of intracellular Ca2+: a model experiment. Life Sciences. 1;83(5-6):164-9.
Saponara, R., & Bosisio, E. (1998). Inhibition of cAMP-phosphodiesterase by biflavones of Ginkgo biloba in rat adipose tissue. J Nat Prod. 61:1386–7.
Shi, C., Zhao, L., Zhu, B., Li, Q., Yew, D. T., Yao, Z., & Xu, J. (2009). Protective effects of Ginkgo biloba extract (EGb761) and its constituents quercetin and ginkgolide B against beta-amyloid peptideinduced toxicity in SH-SY5Y cells. Chem Biol Interact. 181:115–123.
Shibata, S., Satake, N., Morikawa, M., Kown, S. C., Karaki, H., Kurahashi, K., & Kodama, I. (1991). The inhibitory action of okadaic acid on mechanical responses in guinea-pig vas deferens. European Journal of Pharmacology, 193(1), 1–7.
Singh, S. L., Srivastav, S., Castellani, R. J., Plascencia-villa, G., & Perry, G. (2019). Neuroprotective and Antioxidant Effect of Ginkgo biloba Extract Against AD and Other Neurological Disorders. Neurotherapeutics. 16(3):666-674.
Smith, P. F., Maclennan, K. ,& Darlington, C. L. (1996). The neuroprotective properties of the Ginkgo biloba leaf a review of the possible relationship to platelet-activating factor (PAF) J. Ethnopharmacol. 50:131–139.
Waldinger, M. D. (2018): Drug treatment options for premature ejaculation. Expert Opinion on Pharmacotherapy, 19:10, 1077-1085,
Wallace, A., Gabriel, D., Mchale, N. G., Hollywood, M., Athornbury, K. D., & Gerard, P. S. (2015). Regulation of nerve-evoked contractions of rabbit vas deferens by acetylcholine. Physiol Rep. 3(9): e12520.
Westfall, D. P., Stitzel, R. E., & Rowe, J. N. (1978) The postjunctional effects and neural release of purine compounds in the guinea-pig vas deferens. Eur. J. Pharmacol. 50: 27–32.
Wu, Y. N., Liao, C. H., Chen, K. C., Liu, S. P., & Chiang, H. S. (2015). Effect of Ginkgo biloba Extract (EGb-761) on Recovery of Erectile Dysfunction in Bilateral Cavernous Nerve Injury Rat Model. Urology, 85(5), 1214.e7–1214.e15.
Yoshioka, M., Ohnishi, N., Sone, N., Egami, S., Takara, K., Yokoyama, T., & Kuroda, K. (2004). Studies on interactions between functional foods or dietary supplements and medicines. III. Effects of Ginkgo biloba leaf extract on the pharmacokinetics of nifedipine in rats. Biol Pharm Bull. 27(12):2042-5.
Zhang, L., Li, G., Tao, S., Xia, P., Chaudhry, N., Kaura, S., Stone, S. S., & Liu, M. (2022). Ginkgo Biloba Extract Reduces Cardiac and Brain Inflammation in Rats Fed a HFD and Exposed to Chronic Mental Stress through NF-κB Inhibition. Mediators Inflamm. 29;2022:2408598.
Zhang, L., Zhang, X., Li, Q., Xiao, W., Su, E., Cao, F., & Zhao, L. (2021). Optimizing the Desorption Technology of Total Flavonoids of Ginkgo Biloba from Separating Materials of Activated Carbon. ACS Omega. 10;6(50):35002-35013.
Zygmuntowicz, A., Markiewicz, W., Grabowski, T., Burmańczuk, A., Vyniarska, A., & Jaroszewski, J. J. (2021). Quercetin affects uterine smooth muscle contractile activity in gilts. PLoS One.16;16(7):0252438.Colocar espaço entre uma referência e outra
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