Use of whey protein, combined or not with vitamin D and resistant exercise in the treatment of sarcopenia in older adults: an integrative review




Sarcopenia; D vitamin; Whey protein; Aging; Resistance exercise.


To perform an integrative review on the use of whey protein, combined or not with vitamin D supplementation and resistance exercise in the improvement of sarcopenia parameters in older adults. This is an integrative review study carried out in the MedLine, Virtual Health Library and PubMed databases, in which randomized, double-blind, controlled and multicenter clinical trials were selectedTwelve studies that investigated the intervention in the control versus placebo groups were evaluated. Whereas 10 related the use of vitamin D-enriched whey protein and 2 studies evaluated the effects of whey protein. As for the association with resistance exercise (RE), 8 of these studies were associated with RE and 4 studies did not. Satisfactory results have been described in the effects of sarcopenia Z score, increase in muscle strength parameters, muscle mass, fat-free mass and physical performance. There are benefits of isolated whey protein supplementation in improving muscle mass, strength and physical performance in elderly patients with sarcopenia, when combined with vitamin D. Protein supplementation associated with RE showed advantageous results for sarcopenia in aging, potentiated with the effect of supplementation.


Abiri, B., & Vafa, M. (2019). Nutrition and sarcopenia: A review of the evidence of nutritional influences. Critical reviews in food science and nutrition, 59(9), 1456–1466.

Bauer, J. M., Verlaan, S., Bautmans, I., Brandt, K., Donini, L. M., Maggio, M., McMurdo, M. E., Mets, T., Seal, C., Wijers, S. L., Ceda, G. P., De Vito, G., Donders, G., Drey, M., Greig, C., Holmbäck, U., Narici, M., McPhee, J., Poggiogalle, E., Power, D., & Cederholm, T. (2015). Effects of a vitamin D and leucine-enriched whey protein nutritional supplement on measures of sarcopenia in older adults, the PROVIDE study: a randomized, double-blind, placebo-controlled trial. Journal of the American Medical Directors Association, 16(9), 740–747.

Bloom, I., Shand, C., Cooper, C., Robinson, S., & Baird, J. (2018). Diet Quality and Sarcopenia in Older Adults: A Systematic Review. Nutrients, 10(3), 308.

Bo, Y., Liu, C., Ji, Z., Yang, R., An, Q., Zhang, X., You, J., Duan, D., Sun, Y., Zhu, Y., Cui, H., & Lu, Q. (2019). A high whey protein, vitamin D and E supplement preserves muscle mass, strength, and quality of life in sarcopenic older adults: A double-blind randomized controlled trial. Clinical nutrition, 38 1, 159-164.

Brazilian Society of Parenteral and Enteral Nutrition. BRASPEN Journal: Diretriz BRASPEN de Terapia Nutricional no Envelhecimento. ISSN 2525-7374. 3° Suplemento Diretrizes/2019.

Cacchione, P. Z. (2016). The evolving methodology of scoping reviews. Clin Nurs Res; 25:115.

Chanet A, Verlaan S, Salles J, et al. (2017) Supplementing Breakfast with a Vitamin D and Leucine-Enriched Whey Protein Medical Nutrition Drink Enhances Postprandial Muscle Protein Synthesis and Muscle Mass in Healthy Older Men. J Nutr. 2017;147(12):2262-2271. doi:10.3945/jn.117.252510.

Cruz-Jentoft, A. J., Bahat, G., Bauer, J., Boirie, Y., Bruyère, O., Cederholm, T., Cooper, C., Landi, F., Rolland, Y., Sayer, A. A., Schneider, S. M., Sieber, C. C., Topinkova, E., Vandewoude, M., Visser, M., Zamboni, M., & Writing Group for the European Working Group on Sarcopenia in Older People 2 (EWGSOP2), and the Extended Group for EWGSOP2 (2019). Sarcopenia: revised European consensus on definition and diagnosis. Age and ageing, 48(1), 16–31.

Deutz, N. E., Bauer, J. M., Barazzoni, R., Biolo, G., Boirie, Y., Bosy-Westphal, A., Cederholm, T., Cruz-Jentoft, A., Krznariç, Z., Nair, K. S., Singer, P., Teta, D., Tipton, K., & Calder, P. C. (2014). Protein intake and exercise for optimal muscle function with aging: recommendations from the ESPEN Expert Group. Clinical nutrition (Edinburgh, Scotland), 33(6), 929–936.

Englund, D. A., Kirn, D. R., Koochek, A., Zhu, H., Travison, T. G., Reid, K. F., von Berens, Å., Melin, M., Cederholm, T., Gustafsson, T., & Fielding, R. A. (2017). Nutritional Supplementation With Physical Activity Improves Muscle Composition in Mobility-Limited Older Adults, The VIVE2 Study: A Randomized, Double-Blind, Placebo-Controlled Trial. The journals of gerontology. Series A, Biological sciences and medical sciences, 73(1), 95–101.

Fielding, R. A., Travison, T. G., Kirn, D. R., Koochek, A., Reid, K. F., von Berens, Å., Zhu, H., Folta, S. C., Sacheck, J. M., Nelson, M. E., Liu, C. K., Åberg, A. C., Nydahl, M., Lilja, M., Gustafsson, T., & Cederholm, T. (2017). Effect of Structured Physical Activity and Nutritional Supplementation on Physical Function in Mobility-Limited Older Adults: Results from the VIVE2 Randomized Trial. The journal of nutrition, health & aging, 21(9), 936–942.

Ganapathy, A., & Nieves, J. W. (2020). Nutrition and Sarcopenia-What Do We Know?. Nutrients, 12(6), 1755.

Galvão, C. M. (2006). Níveis de evidência. Acta Paulista de Enfermagem; 19(2): 1-5.

Granic, A., Sayer, AA, & Robinson, SM (2019). Padrões alimentares, saúde do músculo esquelético e sarcopenia em adultos mais velhos. Nutrientes, 11 (4), 745.

Kang, L., Gao, Y., Liu, X., Liang, Y., Chen, Y., Liang, Y., Zhang, L., Chen, W., Pang, H., & Peng, L. N. (2019). Effects of whey protein nutritional supplement on muscle function among community-dwelling frail older people: A multicenter study in China. Archives of gerontology and geriatrics, 83, 7–12.

Kemmler, W., Weissenfels, A., Teschler, M., Willert, S., Bebenek, M., Shojaa, M., Kohl, M., Freiberger, E., Sieber, C., & von Stengel, S. (2017). Whole-body electromyostimulation and protein supplementation favorably affect sarcopenic obesity in community-dwelling older men at risk: the randomized controlled FranSO study. Clinical interventions in aging, 12, 1503–1513.

Liberman, K., Njemini, R., Luiking, Y., Forti, L. N., Verlaan, S., Bauer, J. M., Memelink, R., Brandt, K., Donini, L. M., Maggio, M., Mets, T., Wijers, S., Sieber, C., Cederholm, T., & Bautmans, I. (2019). Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study. Aging clinical and experimental research, 31(6), 845–854.

Mastaglia, S. R., Seijo, M., Muzio, D., Somoza, J., Nuñez, M., & Oliveri, B. (2011). Effect of vitamin D nutritional status on muscle function and strength in healthy women aged over sixty-five years. The journal of nutrition, health & aging, 15(5), 349–354.

Moher, D., Liberati, A., Tetzlaff, J., Altman, D. G., & PRISMA Group (2009). Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS medicine, 6(7), e1000097.

Nabuco, H., Tomeleri, C. M., Sugihara Junior, P., Fernandes, R. R., Cavalcante, E. F., Antunes, M., Ribeiro, A. S., Teixeira, D. C., Silva, A. M., Sardinha, L. B., & Cyrino, E. S. (2018). Effects of Whey Protein Supplementation Pre- or Post-Resistance Training on Muscle Mass, Muscular Strength, and Functional Capacity in Pre-Conditioned Older Women: A Randomized Clinical Trial. Nutrients, 10(5), 563.

Paddon-Jones, D., Campbell, W. W., Jacques, P. F., Kritchevsky, S. B., Moore, L. L., Rodriguez, N. R., & van Loon, L. J. (2015). Protein and healthy aging. The American journal of clinical nutrition, 101(6), 1339S–1345S.

Robinson, S. M., Reginster, J. Y., Rizzoli, R., Shaw, S. C., Kanis, J. A., Bautmans, I., Bischoff-Ferrari, H., Bruyère, O., Cesari, M., Dawson-Hughes, B., Fielding, R. A., Kaufman, J. M., Landi, F., Malafarina, V., Rolland, Y., van Loon, L. J., Vellas, B., Visser, M., Cooper, C., & ESCEO working group (2018). Does nutrition play a role in the prevention and management of sarcopenia? Clinical nutrition (Edinburgh, Scotland), 37(4), 1121–1132.

Rondanelli, M., Klersy, C., Terracol, G., Talluri, J., Maugeri, R., Guido, D., Faliva, M. A., Solerte, B. S., Fioravanti, M., Lukaski, H., & Perna, S. (2016). Whey protein, amino acids, and vitamin D supplementation with physical activity increases fat-free mass and strength, functionality, and quality of life and decreases inflammation in sarcopenic elderly. The American journal of clinical nutrition, 103(3), 830–840.

Stuart M Phillips, Nutritional Supplements in Support of Resistance Exercise to Counter Age-Related Sarcopenia, Advances in Nutrition, 6(4), 452–460,

Tessier, A. J., & Chevalier, S. (2018). An Update on Protein, Leucine, Omega-3 Fatty Acids, and Vitamin D in the Prevention and Treatment of Sarcopenia and Functional Decline. Nutrients, 10(8), 1099.

Verreijen, A. M, Verlaan, S., Engberink, M. F, Swinkels, S., de Vogel-van den Bosch, J., & Weijs, P. J. (2015). Um suplemento enriquecido com proteína de soro de leite, leucina e vitamina D preserva a massa muscular durante a perda de peso intencional em idosos obesos: um estudo controlado randomizado duplo-cego. The American Journal of Clinical Nutrition, 101 (2), 279–286.

Verlaan, S., Maier, A. B, Bauer, JM, Bautmans, I., Brandt, K., Donini, L. M, Maggio, M., McMurdo, M., Mets, T., Seal, C., Wijers, S., Sieber, C., Boirie, Y., & Cederholm, T. (2018). Níveis suficientes de 25-hidroxivitamina D e ingestão de proteínas necessárias para aumentar a massa muscular em idosos sarcopênicos - O estudo PROVIDE. Nutrição clínica (Edimburgo, Escócia), 37 (2), 551-557.

Volkert, D., Beck, A. M., Cederholm, T., Cruz-Jentoft, A., Goisser, S., Hooper, L., Kiesswetter, E., Maggio, M., Raynaud-Simon, A., Sieber, C. C., Sobotka, L., van Asselt, D., Wirth, R., & Bischoff, S. C. (2019). ESPEN guideline on clinical nutrition and hydration in geriatrics. Clinical nutrition (Edinburgh, Scotland), 38(1), 10–47.

Whittemore, R., & Knafl, K. (2005). The integrative review: updated methodology. Journal of advanced nursing, 52(5), 546–553.



How to Cite

SILVEIRA, T. M. .; TEIXEIRA, E. de S. .; NASCIMENTO , C. A. do; OLIVEIRA, C. C. de. Use of whey protein, combined or not with vitamin D and resistant exercise in the treatment of sarcopenia in older adults: an integrative review . Research, Society and Development, [S. l.], v. 11, n. 11, p. e347111133645, 2022. DOI: 10.33448/rsd-v11i11.33645. Disponível em: Acesso em: 6 oct. 2022.



Review Article