Evaluation of sexed semen deposition site on the in vivo embryo production of Nelore breed
DOI:
https://doi.org/10.33448/rsd-v11i12.33860Keywords:
Bos indicus; Body; Horn; Superovulation.Abstract
This study’s aim was to verify if the site of sexed semen deposition influences the quantity and quality of embryos from superovulated Nelore cows. Twelve females of proved ciclicity were used and went through SOV, FTAI and embryo collection protocols. FTAI was performed at 18 and 30h after the gonadorelin shot, with two straws each one containing 2,1x106 sperm cells, and the embryo collection was done seven days after the gonadorelin shot. Two repetitions were done, in a cross over design, according to semen deposition site, body or uterine horns. At the end of the treatment, 12.00 ± 2.95 and 12.25 ± 4.37 follicles were identified, with mean diameter of 8.64 ± 2.46 and 8.73 ± 2.42mm, for the groups inseminated in the uterine body and horns, respectively. It was noted that 66.7% (8/12) of the animals had CLs at the day of FTAI, presenting four animals in each group. The group inseminated in the uterine body had embryo recovery rate of 41.07% (23/56) and the group inseminated in the uterine horns had 35.71% (15/42). It was seen a decrease in the embryo recovery rate between repetitions, where the first repetition achieved 44.26% (27/61) while the second showed embryo recovery rate of 29.72% (11/37). Yielded 52.17% (12/23) of viable embryos for the group inseminated in the uterine body and 60% (9/15) for the group inseminated in the uterine horns. It was concluded that there weren’t differences at quantity or quality of recovered embryos according to the sexed semen deposition site at the time of FTAI.
References
Andersson, M., Taponen, J., Koskinen, E., & Dahlbom, M. (2004). Effect of insemination with doses of 2 or 15 million frozen-thawed spermatozoa and semen deposition site on pregnancy rate in dairy cows. Theriogenology, 61, 1583-1588.
Baruselli, P. S., Marques, M. O., Reis, E. L., Nasser, L. F. T., Silva, R. C. P., Menegatti, J. A., Valentin, R., & Santos, I. C. C. (2003). Adequação da dose de FSH (Folltropin-V) em protocolos de superovulação de vacas nelore (Bos taurus indicus) com inseminação artificial em tempo fixo. Acta Scientia Veterinae, 31, 244-245.
Baruselli, P. S., Sá Filho, M. F., Martins, C. M., Nasser, L. F., Nogueira, M. F. G., Barros, C. M., & Bó, G. A. (2006). Superovulation and embryo transfer in Bos indicus cattle. Theriogenology, 65, 77 -88.
Bó, G. A., Adams, G. P., Pierson, R. A., & Mapletoft, R. J. (1996). Effect of progestogen plus E-17b treatment on superovulatory response in beef cattle. Theriogenology, 45, 897 – 910.
Boland, M. P., Goulding, D., & Roche, J. F. (1991). Alternative gonadotrophins for superovulation in cattle. Theriogenology, 35, 5-17.
Carvalho, J. B. P., Carvalho, N. A. T., Reis, E. L., Nichic, M., Souza, A. H., & Baruselli P. S. (2008). Effect of early luteolysis in progesterone-based timed AI protocols in Bos indicus, Bos indicus × Bos taurus, and Bos taurus heifers. Theriogenology, 69, 167-175.
Carvalho, J. O., Sartori, R., Machado, G. M., Mourão, G. B., & Dode M. A. N. (2010). Quality assessment of bovine cryopreserved sperm after sexing by flow cytometry and their use in in vitro embryo production. Theriogenology, 74, 1521-1530.
Galli, C., Duchi, R., Grotti, G., Turini, P., Ponderato, N., Colleoni, S., Lagutina, I., & Lazzari, G. (2003).Bovine embryo technologies. Theriogenology, 59, 599-616.
Gonçalves, P. B. D., Figueiredo, J. R., & Freitas, V. J. F. (2008). Biotécnicas aplicadas à Reprodução Animal, (2ª ed.), Ed. Roca, 398.
Ginther, O. J., Knopf, L., & Kastelic, J. P. (1989).Temporal associations among ovarian events in cattle during oestrous cycles with two and three follicular waves. Journal of Reproduction and Fertility, 87, 223-230.
Hayakawa, H., Hirai, T., Takimoto, A., Ideta, A., & Aoyagi, Y. (2009). Superovulation and embryo transfer in Holstein cattle using sexed sperm. Theriogenology, 71, 68-73.
Hyttel, P., Callesen, H., Greve, T., & Schmidt, M. (1991). Oocyte maturation and sperm transport in superovulated cattle. Theriogenology, 35, 91-108.
IBGE. (2014). http://economia.estadao.com.br/noticias/economia-brasil,rebanho-bovino-brasileiro-e-o-segundo-maiordomundo,167062,0.htm.
Johnson, L. A. (2000). Sexing mammalian sperm for production of offspring: the state-of-the-art. Animal Reproduction Science, 61, 93-107.
Kafi, M., & McGowan, M. R. (1997).Factors associated with variation in the superovulatory response of cattle. Animal Reproduction Science, 48, 137-157.
Kurykin, J., Jaakma, Ü., Jalakas, M., Aidnik, M., Waldmann, A., & Majas, L. (2007). Pregnancy percentage following deposition of sex-sorted sperm at different sites within the uterus in estrus-synchronized heifers. Theriogenology, 67, 754-759.
Larson, J. E., Lamb, G. C., Funnell, B. J., Bird, S., Martins, A., & Rodgers, J. C. (2010). Embryo production in superovulated Angus cows inseminated four times with sexed-sorted or conventional, frozen-thawed semen. Theriogenology, 73, 698-703.
MAPA. (2013) http://www.agricultura.gov.br/animal/especie/bovinosebubalinos.
Martins, C. M., Rodrigues, C. A., Vieira, L. M., Mapletoft, R. J., Bó, G. A., Sá Filho, M. F., & Baruselli, P. S. (2012). The effect of timing of the induction of ovulation on embryo production in superstimulated lactating Holstein cows undergoing fixed-time artificial insemination. Theriogenology, 78, 974-980.
Monteiro Jr, P. L. J., Batista, A. M., Silva, F. L. M., Silva, S. V., Carneiro, G. F., & Guerra, M. M. P. (2011). Produção in vivo de embriões bovinos a partir de sêmen sexado. Ciência Veterinária nos Trópicos, v. 14, p. 20-28, 2011.
Rath, D., Sieg, B., Leigh, J., Klinc, P., Besseling, M., Krüger, C., Wolken, A., Frenzel, A., Westermann, P., Probst, S., Grobfeld, R., Hadeler, K. G., & Ehling, C. (2003). Current prospectives of sperm sorting in domestic farm animals. 19th Meeting Association Europeenne de Transfert Embryonnaire. Proceeding…125-28.
Schenk, J. L., Suh, T. K., & Seidel Jr., G. E. (2006). Embryo production from superovulated cattle following insemination of sexed sperm. Theriogenology, 65, 299-307.
Schenk, J. L., Cran, D. G., Everett, R. W., & Seidel Jr., G. E. (2009). Pregnancy rates in heifers and cows with cryopreserved sexed sperm: effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting. Theriogenology, 71, 717-728.
Seidel Jr., G. E., & Garner, D. L. (2002). Sexing mammalian sperm by flow cytometry. Reproduction, 124, 733-743.
Soares, J. G., Martins, C. M., Carvalho, N. A. T., Nicacio, A. C., Abreu-Silva, A. L., Campos Filho, E. P., Torres Júnior, J. R. S., Sá Filho, M. F., & Baruselli, P. S. (2011). Timing of insemination using sex-sorted sperm in embryo production with Bos indicus and Bos taurus superovulated donos. Animal Reproduction Science, 127, 148-153.
Stringfellow, D. A., & Seidel, S. M. (1998). Manual da Sociedade Internacional de Transferência de Embriões. IETS, p. 112 – 113, Illinois.
Teixeira, H. C. A., Mariante, A. S., Nascimento, N. V., Driessen, K., & Ramos, A. F. (2013). Protocols with Different Time of Progesterone Exposure on Superestimulatory Response and Embryo Production of Locally Adapted Curraleiro Pé-Duro Cows. Journal of Animal Science Advances, 3, 261-269.
Thomazi, S., Pinto-Neto, A., Silva, R. Z., Mota, M. F., Mello, N. M., & Fonseca, J. F. Dinâmica ovariana e concentração de progesterona de vacas Nelore submetidas à IATF. Arquivos de Ciências Veterinárias e Zoologia da UNIPAR, 12(2), 135 – 140, 2009.
Verberckmoes, S., Van Soom, A., De Pauw, I., Dewulf, J., Vervaet, C., & De Kruif, A. (2004). Assessment of a new utero-tubal junction device in dairy cattle. Theriogenology, 61, 103 – 115.
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