Chemical composition and biological activity of propolis from Melipona quadrifasciata

Authors

DOI:

https://doi.org/10.33448/rsd-v11i12.34175

Keywords:

Meliponines; Stingless bee; Phenolics; Carotenoids; Volatile compounds.

Abstract

Propolis is a viscous product produced and collected by worker bees from shots or plant exudates used for the protection of hive. The aims of this work were to perform the phytochemical profile and biological activity of propolis from stingless bee (SB) Melipona quadrifasciata (Mandaçaia) native from Rio Grande do Sul, Brazil. The propolis had a total phenolic concentration of 126,52 mg g-1, and the major phenolic compounds identified were p-coumaric acid (35,64 mg g-1) and gallic acid (18,33 mg g-1). The carotenoids β-carotene, β-criptoxanthin, lutein and lycopene were identified in the propolis of M. quadrifasciata, and β-carotene showed the highest concentration (1.56 mg/100g). The headspace analysis of volatile compounds identified the highest concentration of α-pinene, followed by limonene, sylvestrene and β-pinene. The propolis of M. quadrifasciata showed high antioxidant activity by the DPPH method, with EC50 of 2,29 µg mL-1, antimicrobial activity (MIC) against Escherichia coli and Staphylococcus aureus and low reduction in cell viability of cell lines L929, GL, A549, MDA and SKMEL.

References

Adams, R. P. (2017). Identification of essential oil components by gas chromatography/mass spectrometry. Allured business media, 4.1.

Bankova, V., Christov, R., Popov, S., Marcucci, M. C., Tsvetkova, I., & Kujumgiev, A. (1999). Antibacterial activity of essential oils from Brazilian propolis. Fitoterapia, 70, 190-193.

Bankova, V., Dyulgerov, A., Popov, S., Evstatieva, L., & Kuleva, L. (1992). Propolis produced in Bulgaria and Mongolia: phenolic compounds and plant origin. Apidologie ,23, 79-85.

Bankova, V., Popova, M., & Trusheva, B. (2014). Propolis volatile compounds: chemical diversity and biological activity: a review. Chem. Cent. J., 8-28.

Barboza, J. R., Pereira, F. A. N., Fernandes, R. A., Vasconcelos, C. C., Cartágenes, M. S. S., Lopes, A. J. O., Melo, A. C., Guimarães, I. S., Rocha, C. Q., & Ribeiro, M. N. S. (2020). Cytotoxicity and Pro-Apoptotic, Antioxidant and Anti-Inflammatory Activities of Geopropolis Produced by the Stingless Bee Melipona fasciculata Smith. Biology, 9, 292; doi:10.3390/biology9090292.

Bonamigo, T., Campos, J. F., Alfredo, T. M., Balestieri, J. B. P., Cardoso, C. A. L., Paredes-Gamero, E. J., Souza, K. P., & Santos, E. L. (2017). Antioxidant, cytotoxic, and toxic activities of propolis from two native bees in Brazil: Scaptotrigona depilis and Melipona quadrifasciata anthidioides. Oxid. Med. Cell. Longevity.

Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Technol., 28, 25–30.

Campos, J. F., Santos, U. P., Macorini, L. F. B., Mestriner, A. M., Balestieri, J. B. P., Gamero, E. J. P., Cardoso, C. A. L., Souza, K. P., & Santos, E. L. (2014). Antimicrobial, antioxidant and cytotoxic activities of propolis from Melipona orbignyi (Hymenoptera, Apidae). Food Chem. Toxicol., 65, 374-380.

Cardozo, D. V., Mokochinski, J. B., Schineider, C. M., Sawaya, A. C. H. F., Caetano, I. K., Felsner, M. L., & Torres, Y. R. (2015). Variabilidade química de geoprópolis produzida pelas abelhas sem ferrão jataí, mandaçaia e manduri. Rev. Virtual Quim., 7, 2457-2474.

Cervantes, J. A. C., Martínez, J. G. S., Uribe, A. J. R., Sauceda, M. L. V., Castro, J. L. R., Castañeda, R. P., Martínez, Z. B., Olvera, L. G., & González, F. B. (2020). Effect of propolis, a honeybee product, against a parasite (Ligictaluridus floridanus) from catfish (Ictalurus punctatus). Acta Parasitol..

Cisilotto, J., Sandjo, L. P., Faqueti, L. G., Fernandes, H., Joppi, D., Biavatti, M. W., & Creczynski-Pasa, T. B. (2018). Cytotoxicity mechanisms in melanoma cells and UPLC-QTOF/MS2 chemical characterization of two Brazilian stingless bee propolis: Uncommon presence of piperidinic alkaloids. Journal of Pharmaceutical and Biomedical Analysis, 149 502–511.

Cruz, L. F. S., Santos, T. S., Souza, C. O., Santos, L. S. M., & Druzian, J. I. (2020). Determination of physicochemical characteristics and bioactive compounds in samples of pollen, geopropolis and honey from Melipona Scutellaris bee species. Braz. J. Develop., 6, 21484-21496.

Falcão, S. I., Freire, C., Figueiredo, A. C., & Vilas-Boas, M. (2016). The Volatile Composition of Portuguese Propolis Towards its Origin Discrimination. Rec. Nat. Prod., 10, 176-188.

Ferreira, B. L., Gonzaga, L. V., Vitalib, L., Mickeb, G. A., Maltezc, H. F., Ressureição, C., Costa, A. C. O., & Fetta, R. (2019). Southern-Brazilian geopropolis: A potential source of polyphenolic compounds and assessment of mineral composition. Food Research International, 126 108683.

Frizzo, C. D., Serafini, L. A., Dellacassa, E., Lorenzo, D., & Moyna, P. (2001). Essential oil of Baccharis uncinella DC. from Southern Brazil. Flavour Fragrance J., 16, 286–288.

Gualberto, N. C. (2013). Identificação e quantificação de compostos fenólicos por CLAE-DAD em polpa de frutas de cajá umbu (Spondias spp.) e graviola (Annona muricata L.)). Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Universidade Federal de Sergipe, 122p.

Kasote, D. M., Pawar, M. V., Gundu, S. S., Bhatia, R., Nandre, V. S., Jagtap, S. D., Mahajan, S. G., & Kulkarni, M. V. (2019). Chemical profiling, antioxidant, and antimicrobial activities of Indian stingless bees propolis samples. J. Apicult, Research, 4, 617-625.

Kumar, N., & Goel, N. (2019). Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnology Reports, 24.

Laurino, M. C., Fonseca, V. L. I., Roubik, D. W., Dollin, A., Heard, T., Aguilar, I., Venturieri, G. C., Eardley, C., & Neto, P. N. (2006). Global meliponiculture: challenges and opportunities. Apidologie, 37, 275-292.

Lavinas, F. C., Macedo, E. H. B. C., Sá, G. B. L., Amaral, A. C. F., Silva, J. R. A., Azevedo, M. M. B., Vieira, B. A., Domingos, T. F. S., Vermelho, A. B., Carneiro, C. S., & Rodrigues I. A. (2019). Brazilian stingless bee propolis and geopropolis: promising sources of biologically active compounds. Rev. Bras. Farmacogn., 29, 389-399.

Leonhardt, S. D., Zeilhofer, S., Bluthgen, N., & Schmitt, T. (2010). Chem. Senses, 35, 603-611.

Lou, Z., Wang, H., Rao, S., Sun, J., Ma, C., & Li, J. (2012). p-Coumaric acid kills bacteria through dual damage mechanisms. Food Control, 25(2), 550–554.

Mouhoubi-Tafinine, Z., Ouchemoukh, S. &Tamendjari, A. (2016). Antioxydant activity of some Algerian honey and propolis. Ind. Crops Prod., 18, 85-90.

Pazin, W. M., Mônaco, L. M., Soares, A. E. E., Miguel, F. G., Berretta, A. A., & Ito, A. S. (2017). Antioxidant activities of three stingless bee propolis and green propolis types. J. Apicult. Research, 56, 40-49.

Pino, J. A., Marbot, R., Delgado, A., Zumárraga, C., & Sauri, E. (2006). J. Essent. Oil Res., 18, 53-56.

Popova, M., Trusheva, B. & Bankova, V. (2021). Propolis of stingless bees: A phytochemist’s guide through the jungle of tropical biodiversity. Phytomedicine 86 153098.

Salatino, A., Pereira, L. R. L. ,& Salatino, M. L. F. (2019). The emerging market of propolis of stingless bees in tropical countries. Department of Botany, University of São Paulo, Brazil, 7 Issue 2.

Sanches, M. A., Pereira, A. M. S., & Serrão, J. E. (2017). Pharmacological actions of extracts of propolis of stingless bees (Meliponini). J. Apicult. Research, 56, 50-57.

Santos, C. M., Campos, J. F., Santos, H. F., Balestieri, B. P., Silva, D. B., Souza, K. P., Carollo, C. A., Estevinho, L. M., & Samtos, E. L. (2017). Chemical Composition and Pharmacological Effects of Geopropolis Produced by Melipona quadrifasciata anthidioids. Oxidative Medicine and Cellular Longevity Volume, Article ID 8320804, 13 pag.

Simone-Finstrom, M., & Spivak, M. (2010). Propolis and bee health: the natural history and significance of resin use by honey bees. Apidologie, 41, 295-311.

Souza, S. A., Dias, T. L. M. F., Silva, T. M. G., Falcão, R. A., Moreira, M. S. A., Silva, E. M. S., Camara, C. A., & Silva, T. M. S. (2014). Chemical composition, antinociceptive and free radical-scavenging activities of geopropolis from Melipona subnitida Ducke (Hymenoptera: Apidae: Meliponini). Sociobiology, 61, 560-565.

Torres, A. R., et. al. (2018). Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees. Braz. J. Med. Biol. Res., 51, 1-10.

Torres, A. R., Sandjo, L. P., Friedemann, M. T., Tomazzoli, M. M., Maraschi, M., Mello, C. F., & Santos, A. R. S (2018). Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees. Brazilian Journal of Medical and Biological Research, 51(6): e7118, http://dx.doi.org/10.1590/1414-431X20187118.

Witter, S., & Blochtein, B. (2008). Espécies de abelhas sem ferrão de ocorrência no Rio Grande do Sul. Versátil Artes Gráficas, Porto Alegre – BR, 1.

Published

11/09/2022

How to Cite

PICCININI, A.; SOUSA, M. H. O. de; FREITAS, M. dos S. D.; CESCA, K.; MOURA, N. F. de. Chemical composition and biological activity of propolis from Melipona quadrifasciata. Research, Society and Development, [S. l.], v. 11, n. 12, p. e193111234175, 2022. DOI: 10.33448/rsd-v11i12.34175. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/34175. Acesso em: 12 nov. 2024.

Issue

Section

Exact and Earth Sciences