Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract

Authors

DOI:

https://doi.org/10.33448/rsd-v11i12.34326

Keywords:

Amazon; Endophyte; Biocatalysis; Endomelanconiopsis endophytica; Biodiesel.

Abstract

Endophytic fungi (EF) have a notable capacity to produce active molecules of industrial importance, such as hydrolytic enzymes. In this study we investigated the production of lipase by EFs isolated from the Amazonian species Aniba canelilla (Lauraceae), characterized the enzymatic extract obtained from the most promising fungus, and applied the lipolytic extract as a biocatalyst in the transesterification reaction for biodiesel production. The fungi were submitted to enzymatic screening in solid medium and in submerged fermentation to assess their lipase production. A total of 292 fungi were tested in solid media. Lipolytic activity was detected in 74% of the fungi cultivated in liquid media, 18 of which showing promising enzymatic production. The best lipase producer, Endomelanconiopsis endophytica QAT_7AC, was identified by sequencing of the ITS region. After adjusting the bioprocess conditions, E. endophytica QAT_7AC produced 2,415.5 U/mL of lipase after 72 h. The enzymatic extract showed higher lipolytic activity under pH 8.0 and 40 oC. The extract was applied as a biocatalyst in a transesterification reaction performed at 40 oC, with ethanol and waste cooking oil (3:1). The biodiesel yield was found to be 87% after 2 h rection when the fungal enzyme was used and 89% with the commercial biocatalyst. The endophytic fungi isolated from A. canelilla proved themselves to be biotechnologically relevant, as they can be explored as potential producers of lipases. The lipolytic extract can be applied in the synthesis of biodiesel using waste cooking oil.

Author Biographies

Rosiane Rodrigues Matias, Universidade do Estado do Amazonas

Graduate Program in Biodiversity and Biotechnology - Bionorte, School of Health Sciences

Juliana Gisele Corrêa Rodrigues, Universidade do Estado do Amazonas

Graduate Program in Biodiversity and Biotechnology - Bionorte, School of Health Sciences

Rudi Emerson de Lima Procópio, Universidade do Estado do Amazonas

Graduate Program in Biotechnology and Amazon Natural Resources, School of Health Sciences

Carla Roberta Matte, Federal University of Rio Grande do Sul

Laboratory of Biotechnology and Biochemistry Engineering, Institute of Science and Food Technology

Sergio Duvoisin Junior, Amazonas State University

Laboratory of Chemistry Applied to Technology, School of Technoloy

Marco Antonio Zachia Ayub, Federal University of Rio Grande do Sul

Laboratory of Biotechnology and Biochemistry Engineering, Institute of Science and Food Technology

Rosane Michele Duarte Soares, Federal University of Rio Grande do Sul

Laboratory of Polymeric Biomaterial, Institute of Chemistry

Patrícia Melchionna Albuquerque, Universidade do Estado do Amazonas

Graduate Program in Biodiversity and Biotechnology - Bionorte, School of Health Sciences

Graduate Program in Biotechnology and Amazon Natural Resources, School of Health Sciences

Laboratory of Chemistry Applied to Technology, School of Technology

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10/09/2022

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MATIAS, R. R.; RODRIGUES, J. G. C.; PROCÓPIO, R. E. de L.; MATTE, C. R. .; DUVOISIN JUNIOR, S.; AYUB, M. A. Z.; SOARES, R. M. D.; ALBUQUERQUE, P. M. Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract. Research, Society and Development, [S. l.], v. 11, n. 12, p. e180111234326, 2022. DOI: 10.33448/rsd-v11i12.34326. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/34326. Acesso em: 16 oct. 2024.

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Agrarian and Biological Sciences