Pharmacognostic Study, Phytochemical and Toxicity Assessment of Justicia secunda vahl
DOI:
https://doi.org/10.33448/rsd-v11i12.34344Keywords:
Sanguine; Toxic; In silico study.Abstract
The study by objective describes the results of the quality studies. The leaves were dried and pulverized, and the powders were selected for pharmacognostic tests for pH, foam index, moisture determination and density. From maceration of maceration with ethanol, the extract of the leaves obtained (E) and through the fractions of maceration, dichlorom of half hexanol, followed by prospection phytoethanolic by half-ethane and chemical reflux methane by reflux. For in silico studies, Luteolin 7-[βglucopyranosyl]-(1→2)-βramnosyl-(1→6)β-glucopyranoside, Luteolin-7-O Rutinoside, β-Sitosterol, Campesterol, Friedelinol , lupeol, justiflorinol and apigenin the programs Marvin JS, PreADMETox and OSIRIS were used to evaluate the toxicological aspects of the species associated with the in vitro test against Artemia Salina L. of humidity within the parameters recommendations, with low density and neutral pH. In phytochemical analysis, flavonoids, coumarins, terpenes and steroids, tannins and depsones were detected. Among the non-genic genetics, β -S-Campesterol, Friedelinolforam, apigenin and luteolin7-O-carcinoside are not carcinogens. The EEF and the fractions showed levels of toxicity against the Artemia salina test.
References
Ajuru, M. G., Kpekot, A. K., Omubo, J., & Morrison, I. (2021). Comparative Study of Proximate and Phytochemical Analysis of the Roots of Justicia carnea Lindi. and Justicia secunda Vahl. Nigerian Annals Of Pure And Applied Sciences, 4 (1), 22-33. https//doi.org/10.46912/napas.217. Doi: https//doi.org/10.46912/napas.217.
Amarante, C. B., Müller, A. H., Póvoa, M. M., & Dolabela, M. F. (2011). Estudo fitoquímico biomonitorado pelos ensaios de toxicidade frente à Artemia salina e de atividade antiplasmódica do caule de aninga (Montrichardia linifera). Acta Amazônica, 41(3), 431-4.
Ames, B. N., Mccann, J. E., & Yamasaki, E. (1975). Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity test*. Mutation Research. 31, 347-64. 10.1016/0165-1161(65)90046-1.
Anarado, C., Ajiwe, V. I. E., Anarado, C. J. O., Obumselu, T. U., & Okafor, S. E. (2021). Phytochemical, Proximate, In-vitro Antimalarial and Antimicrobial screening of leaf Extracts of Justicia carnea Lindl. International Research Journal of Pure & Applied Chemistry. 10.9734/IRJPAC/2021/v22i830428
Aragón, I. M. D. (2020). Justicia secunda Valh, especie utilizada e la Medicina Indígena colombiana. Trabalho de Conclusão de Curso (TCC). Graduação em Farmácia. https://idus.us.es/bitstream/handle/11441/103953/DOMINGUEZ%20ARAGON%20ISABEL.pdf?sequence=1&isAllowed=y.
Araújo, F. A. De S., Rocha, R. G. De A., Campos, L. A. De A., Moura, D. F., Pereira, M. A., Martins, R. D., & Cavalcanti, I. M. F. (2021).Action of Justicia pectoralis Jacq. (Acanthaceae) in the treatment of respiratory tract infections: a literature review. Research, Society and Development, [S. l.], 10 (16), e26101623152. 10.33448/rsd-v10i16.23152.
Ayodele, A. E., Odusole, O. I., & Adekanmbi, A. O. (2020). Phytochemical screening and in-vitro antibacterial activity of leaf extracts of Justicia secunda Vahl on selected clinical pathogens. MicroMedicine, 8 (2), 46-54. http://www.journals.tmkarpinski.com/index.php/mmed/article/view/275
Barbosa, W. L. R. (2004). Manual para análise fitoquímica e cromatográfica de extratos vegetais. Revista Cientifica da UFPA. Belém-PA. Vol.4.
Brasil. (2019) Farmacopeia Brasileira, volume 1 e 2 (6º Ed.), Anvisa..
Cantillo, J., Guette, J., Baldiris, R., Jaramillo, B., & Olivero, J. (2007). Evaluación de la toxicidad aguda (CL50) frente a Artemia franciscana y la actividad hemolitica de los extractos acuosos, en diclorometano y metanólico parcial de Justicia secunda (Vahl.). Scientia et technica, 13 (33), 257-8. https://doi.org/10.22517/23447214.6093
Cardoso, I. C., Pereira, H. M.G., & Tappin, M. R. R. (2017). Influence of extraction technique and particle size of the plant material in the content of total phenolic compounds of tincture of Alpinia zerumbet leaves Rev. Fitos. 11 (1), 62-8. 10.5935/2446-4775.20170016
Cidade, A. F., Rodrigues, D., Santos, E. B. S., Rocha, E. P. S., Oliveira, G. C., Macedo, J. M., De Oliveira, J. V. S., De Mesquita, J. S., Pereira, M. V. D., Da Silva, M. A., De Araújo, N. F., Taborda, R. L. M., & Rodrigues, R. C. (2018). Caracterização Físico-Química E Microbiológica De Peperomia quadrangulares. http://www.sbpcnet.org.br/livro/70ra/trabalhos/resumos/2340_138e010be804de69531c4063b5846dd1a.pdf
Costa, C. R., Olivi, P., Botta, C. M. R., & Espindola, E. L. G. (2008). A toxicidade em ambientes aquáticos: discussão e métodos de avaliação. Química nova. 3(7), 1820-30. https://www.scielo.br/j/qn/a/X6sRQb5cdDnHxgPJvZR33PN/?lang=pt
Do Nascimento Júnior, C. S., Cavalcante, E. B. V. S., de Sousa, A. P., Silva, D. F., & Ferreira, M. D. L (2020). Análise qualitativa do perfil químico de plantas medicinais do horto das Faculdades Nova Esperança. Research, Society and Development, 9 (9), e816998033. http://dx.doi.org/10.33448/rsd-v9i9.8033.
Escobar. M. S. P., & Flores. J. C. T. (2019). Estudio preclínico normo-glucemiante de los extractos de las hojas de insulina (justicia secunda Vahl) en ratones. Tese de Doutorado. Universidad de Guayaquil. Facultad de Ciencias Químicas. http://repositorio.ug.edu.ec/handle/redug/43595.
Guilhermino, L., Diamantino, T., Silva, M. C., & Soares, A. M. V. M. (2000). Acute toxicity test with Daphnia magna: An alternative to mammails in the Prescreening of Chemical Toxicity?. Ecotoxicology and Environmental Safety. 46(3), 357-62. 10.1006/ eesa.2000.1916.
Koffie, E. N., Guernevé, L. C., Lozano, P. R., & Lozano, Y. F. (2013). Polyphenol extraction and characterization of Justicia secundaVahl leaves for traditional medicinal uses. Science Direct. https://doi.org/10.1016/j.indcrop.2013.06.001.
Koffie, E. N., Kassi, A. B. B., Adje, F. A., Lozano, Y, F., & Bekro, Y. A. (2020). Effect of freeze-drying and spray-drying on total phenolics content and antioxidant activity from aqueous extract of Justicia secunda leaves. Trends in Phytochemical Research, 4 (2), 69-76. https://tpr.iau-shahrood.ac.ir/article_672456.html.
López, T., Morillo, M., Visbal, T., & Carmona,J. (2022). Estudio fitoquímico, actividad antioxidante y toxicidad sobre Artemia salina de los extractos de las hojas de Justicia secunda Vahl.(Acanthaceae), recolectada en Mérida-Venezuela. Revista Colombiana de Ciencias Químico-Farmacéuticas, 51 (1). https://revistas.unal.edu.co/index.php/rccquifa/article/view/95333.
Herrera-Mata, H., Rosas-Romero, A., & Crescente, O. V. (2002). Biological activity of “Sanguinaria”(Justicia secunda) extracts. Pharmaceutical biology, 40 ( 3), 206-12. https://doi.org/10.1076/phbi.40.3.206.5826.
Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E., & Mclaughlin, J. L. (1982). Brine Shrimp: A convenient general bioassay for active plant constituents. Journal of medicinal plant research planta medica. 45, 31-4. https://doi.org/10.1055/s-2007-971236.
Neba, N., Ngwa, M., Anuanwen, C., & Bup, D. N. (2020). Extração simultânea e Pasteurização (SEPA) de uma bebida de "raiz sanguínea" (Justicia secunda) deixa a parte 1: estudos cinéticos. Heliyon, 6(2), e03297. https://doi.org/10.1016/j.heliyon.2020.e03297.
Onochie, A. U., Oli, A. H., Oli, A. N., Ezeigwe, O. C., Nwaka, A. C., Okani, C. O., Okam, P. C., Ihekwereme, C. P., & Okyoeh, J. N. (2020). The pharmacobiochemical effects of ethanol extract of justicia secunda vahl leaves in rattus norvegicus. Journal of Experimental Pharmacology, 12, 423. https://doi.org/10.2147/JEP.S267443.
Onoja, O. S., Ezeja, M. I., Omeh, Y. N., & Onwukwe, B. C. (2017). Antioxidant, anti-inflammatory and antinociceptive activities of methanolic extract of Justicia secundaVahl leaf. Alexandria Journal of Medicine. https://doi.org/10.1016/j.ajme.2016.06.001.
Pérez, J. P. L., & Ruiz. E. R. C. (2021). Estudio etnobotánico, químico y antioxidante de Justicia secunda Vahl. Tese de Doutorado em Química e Farmácia. Universidad de Guayaquil. Facultad de Ciencias Químicas. Guayaquil-Ecuador. http://repositorio.ug.edu.ec/handle/redug/53660.
Proventi, L. R. (2018). Estudo fitoquímico e atividade antioxidante de justicia thunbergioides(lindau) Leonard. (acanthaceae). Dissertação de Mestrado em Ciências Ambientais. Centro Universitário de Anapólis, Goiás. http://repositorio.aee.edu.br/jspui/handle/aee/1856.
Quaresma, A. C. S., Melo, P. R. de S., Brandão, D. L. dos N., & Dolabela, M. F.(2021). Farmacognóstico, caracterização fitoquímica e na avaliação silico da atividade de monoterpens isolados da espécie Dispania ambrosioides (L.) Mosyakin & Clemants. Research, Society and Development,, 10(8), e9210816715. https://doi.org/10.33448/rsd-v10i8.16715
Salgueiro, A. C. F., Goulart, da S. A., Viçosa, D. L., Viçosa, C. S. C. L., & Folmer, V. (2018). Resolução de problemas no ensino de Ciências: utilização de Artemia salina como modelo experimental para o estudo de plantas medicinais na escola básica. Revista de Ensino de Bioquímica, 16 (2), 31-47. https://scholar.archive.org/work/t6xx6n7hivdavin2qr4hmsgecu/access/wayback/http://bioquimica.org.br/revista/ojs/index.php/REB/article/download/814/654.
Shamili, G., & Santhi, G. (2019). Identification and characterization of bioactive compounds of leaves of Justicia gendarussa Burm. F. Int. J. Sci. Res. in Biological Sciences 6, 1. https://doi.org/10.26438/ijsrbs/v6i1.145153
Silva, M. C. M. da. (2018). Estudo farmacognóstico, fitoquímico e atividade antiplasmódica de Aspidosperma eteanum Markgr. Dissertação de Mestrado em Ciências Farmacêuticas. Universidade Federal do Pará, Belém .http://repositorio.ufpa.br/jspui/handle/2011/10548
Silva Chagas, C. K., Rolim, C. E. L., Martins, H. P. Dos S., & Dolabela, M. F.(2022) . In silico study of phenolic compounds isolated from Inga laurina. Research, Society and Development, [S. l.], 11 (2), 24511225592. 10.33448/rsdv11i2.25592.
Silva, I. P. Da., Lins, F. A., Costa, L. Dos S., Sobreira, A. L. De C., & Souza, J. B. P. De .(2022). Jatoba tincture (Hymenaea sp.): physico-chemical aspects of quality and assessment of antimicrobial activity. Research, Society and Development, [S. l.], 11 (6), 11111628865. 10.33448/rsd-v11i6.28865.
Theiler, B. A., Revoltella, S., Zehl, M., Dangl, C., Caisa, L. O. E., König, J., & Glasl, S. (2014). Secundarellone A, B e C das folhas de Justicia secunda Vahl. Phytochemistry letters, 10. https://doi.org/10.1016/j.phytol.2014.05.007.
Thirumagal, N., & Jeyakumari, A. P.(2020). Propriedades estruturais, ópticas e antibacterianas de nanopartículas de prata sintetizadas verdes (AgNPs) utilizando extrato de folha justicia adhatoda L. Journal of Cluster Science, 31 (2), 487-97. https://link.springer.com/article/10.1007/s10876-019-01663-z.
Umoh Romanus, A., Imoh, J., Umoh Omodot, T., Udoh Anwanabasi, E., Anah Victor, U., & Obah-Eni Love, C. (2020). Pharmacognostic Evaluation Of The Leaves And Stems Of Justicia Secunda Vahl.(Acanthaceae). World Journal of Pharmaceutical Research. ISSN 2277– 7105.
Venturi, I. Estudo Fitoquímico e Avaliação Biológica de Flores e Frutos de Solanum dipolconos (Mart) Bohs. (2018). Tese de doutorado. Universidade Vale do Rio Doce, Itajai, Santa Catarina. http://siaibib01.univali.br/pdf/Ivonilce%20Venturi.pdf
Vale, V. V., Vilhena, T. C., Trindade, R. C. S., Ferreira, M. R. C., Percário, S., Soares, L. F., Pereira, W. L. A., Brandão, G. C., Oliveira, A. B., Donabela, M. F., & De Vasconcelos, F. (2015). Anti-malarial activity and toxicity assessment of Himatanthus articulatus, a plant used to treat malaria in the Brazilian Amazon. Malaria journal, 14(1), 1-10. https://malariajournal.biomedcentral.com/articles/10.1186/s12936-015-0643-1.
Kakazu, C. S., de Jesus, B. C., Tito, E. R. G., Luciano, L. A., & Silva, E. S. (2020). Identification of physical-chemical and microbiological parameters from obtaining the alcoholic extract (92º) of the walnut leaf (Juglans regia L.). Brazilian Journal of Development, 6 (10), 81185-95. https://www.brazilianjournals.com/index.php/BRJD/article/view/18702.
Wagner, H., Bladt, S., & Zgainski, E. M. (1984). Plant drug analysis. Berlim:Springer.
Zambrano, M. P., & Bustamante, K. E. (2017). Caracterizacion y estudio Fitoquímico de Justicia secunda Valh (Sanguinaria, singamochilla, insulina). Revista Cubana de Plantas Medicinales, 1 (22), 4-6. http://www.revplantasmedicinales.sld.cu/index.php/pla/article/view/494
Zucker, E. (1985). Hazard Evaluation Division Standard Evaluation Procedure: Acute toxicity test for freshwater Fish. Washington, USA: USEPA. https://ntrl.ntis.gov/NTRL/dashboard/searchResults/titleDetail/PB86129277.xhtml.
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Copyright (c) 2022 Camilla Eduarda Lima Rolim; Ana Carolina Sousa Quaresma Quaresma; Cristian Kallahan Silva Chagas; José Otávio Carréra Silva Júnior; Paulo Ricardo de Souza Melo; Maria Fâni Dolabela
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