The first report about Fusarium falciforme that causes root rot in cassava cv Rosinha, in Alagoas state, Brazil

Authors

DOI:

https://doi.org/10.33448/rsd-v11i12.34369

Keywords:

Manihot sp.; Biological control; Molecular biology; Fungus.

Abstract

The aim of this study was identifying the root rot causal agents in cassava, cv Rosinha, in Alagoas state, Brazil. Pathogen species were studied, which are responsible for root rot in cassava, through isolation, morphological characterization and pathogenicity confirmation. Fusarium sp was emphasized among other isolated genera, which is pathogenic to cassava roots and plants. The phylogenetic study was based on the ITS region of the constructed DNA, using the ‘‘maximum Likelihood Tree’’ method, with a base of 1000 “bootstrap” replicas, what showed 100% of genetic similarity to Fusarium falciforme.

References

Alves, R. N. B., Modesto Junior, M. de S., Silva, A. R. B., Nakasone, A. K., & Farias Neto, J. T. de. (2020). Podridões em raízes de mandioca: Problemas e soluções para o seu controle. 455(1a edição), 28f.

Azevedo, P. F. de, Almeida, A. C. de, Marques, R. D., Costa, C. L. da, Benedetti, A. R., Lescano, L. E. A. M., Carvalho, M. C. da C. G., & Matsumoto, L. S. (2021). In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer. Research, Society and Development, 10(3), e5210312994–e5210312994. https://doi.org/10.33448/rsd-v10i3.12994

Baayen, R. P., O’Donnell, K., Bonants, P. J., Cigelnik, E., Kroon, L. P., Roebroeck, E. J., & Waalwijk, C. (2000). Gene Genealogies and AFLP Analyses in the Fusarium oxysporum Complex Identify Monophyletic and Nonmonophyletic Formae Speciales Causing Wilt and Rot Disease. Phytopathology, 90(8), 891–900. https://doi.org/10.1094/PHYTO.2000.90.8.891

Chehri, K., Salleh, B., Yli-Mattila, T., Reddy, K. R. N., & Abbasi, S. (2011). Molecular characterization of pathogenic Fusarium species in cucurbit plants from Kermanshah province, Iran. Saudi Journal of Biological Sciences, 18(4), 341–351. https://doi.org/10.1016/j.sjbs.2011.01.007

Chhetri, G., Pandey, T., Kumar, B., Akhtar, M. S., & Tripathi, T. (2015). Recombinant expression, purification and preliminary characterization of the mRNA export factor MEX67 of Saccharomyces cerevisiae. Protein Expression and Purification, 107, 56–61. https://doi.org/10.1016/j.pep.2014.11.011

Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue—World Vegetable Center. Phytochemical Bulletin, 19(1), 11–15. http://www.sciepub.com/reference/60627

Edgar, R. C. (2004). MUSCLE: Um método de alinhamento de sequências múltiplas com menor tempo e complexidade espacial. BMC Bioinformatics, 5(1), 113. https://doi.org/10.1186/1471-2105-5-113

Hyde, K. D., Nilsson, R. H., Alias, S. A., Ariyawansa, H. A., Blair, J. E., Cai, L., de Cock, A. W. A. M., Dissanayake, A. J., Glockling, S. L., Goonasekara, I. D., Gorczak, M., Hahn, M., Jayawardena, R. S., van Kan, J. A. L., Laurence, M. H., Lévesque, C. A., Li, X., Liu, J.-K., Maharachchikumbura, S. S. N., & Zhou, N. (2014). One stop shop: Backbones trees for important phytopathogenic genera: I (2014). Fungal Diversity, 67(1), 21–125. https://doi.org/10.1007/s13225-014-0298-1

Machado, A. R., Pinho, D. B., Oliveira, S. A. S., & Pereira, O. L. (2014). New occurrences of Botryosphaeriaceae causing black root rot of cassava in Brazil. Tropical Plant Pathology, 39(6), 464–470. https://doi.org/10.1590/S1982-56762014000600008

Nalim, F. A., Samuels, G. J., Wijesundera, R. L., & Geiser, D. M. (2011). New species from the Fusarium solani species complex derived from perithecia and soil in the old world tropics. Mycologia, 103(6), 1302–1330. https://doi.org/10.3852/10-307

Nascimento Junior, N. A. (2015). Efeito da casca de mandioca no controle da podridão radicular causada por Phytophthora sp. Em mandioca de mesa (Manihot esculenta Crantz) var. Rosinha em ambiente irrigado. [Tese de Doutorado. (Programa de Pós-Graduação em Proteção de Plantas da Universidade Federal de Alagoas., UNIVERSIDADE FEDERAL DE ALAGOAS]. https://ceca.ufal.br/pt-br/pos-graduacao/protecao-de-plantas/documentos/dissertacoes-e-teses/doutorado/2015/efeito-da-casca-de-mandioca-no-controle-da-podridao-radicular-causada-por-phytophthora-sp-em-mandioca-de-mesa-manihot-esculenta-crantz-var-rosinha-em-ambiente-irrigado

O’Donnell, K., & Gray, L. (1995). Phylogenetic Relationships of the Soybean Sudden Death Syndrome Pathogen Fusarium solani f. Sp. Phaseoli Inferred from rDNA Sequence Data and PCR Primers for Its Identification. Molecular plant-microbe interactions : MPMI, 8, 709–716. https://doi.org/10.1094/MPMI-8-0709

O’Donnell, K., Rooney, A. P., Proctor, R. H., Brown, D. W., McCormick, S. P., Ward, T. J., Frandsen, R. J. N., Lysøe, E., Rehner, S. A., Aoki, T., Robert, V. A. R. G., Crous, P. W., Groenewald, J. Z., Kang, S., & Geiser, D. M. (2013). Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria. Fungal Genetics and Biology, 52, 20–31. https://doi.org/10.1016/j.fgb.2012.12.004

O’Donnell, K., Sutton, D. A., Fothergill, A., McCarthy, D., Rinaldi, M. G., Brandt, M. E., Zhang, N., & Geiser, D. M. (2008). Molecular Phylogenetic Diversity, Multilocus Haplotype Nomenclature, and In Vitro Antifungal Resistance within the Fusarium solani Species Complex. Journal of Clinical Microbiology, 46(8), 2477–2490. https://doi.org/10.1128/JCM.02371-07

Oliveira, N. T. de, Alves, J. M. A., Uchôa, S. C. P., Rodrigues, G. S., Melville, C. C., & Albuquerque, J. de A. A. de. (2011). Characterization of cassava clones produced in Roraima for in natura consumption. Agro@mbiente On-line, 5(3), 188–193. https://www.cabdirect.org/cabdirect/abstract/20123022085

Paiva, G. F., Souza, G. H. S. de, Alves, A. R., Silva, B. V. S., Mota, L. H. C., Gonçalves, F. J. T., & Carrasco, N. F. (2022). Avaliação de resistência a Fusarium solani em genótipos de mandioca. Research, Society and Development, 11(9), e23911931734–e23911931734. https://doi.org/10.33448/rsd-v11i9.31734

Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D. L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M. A., & Huelsenbeck, J. P. (2012). MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology, 61(3), 539–542. https://doi.org/10.1093/sysbio/sys029

Silva, I. O. (2016). Etiologia da podridão radicular da mandioca de mesa (Manihot esculenta Crantz) var. Rosinha no Estado de Alagoas e Influência de Trichoderma spp. Sobre o desenvolvimento de Fusarium sp [Tese de Doutorado]. Programa de Pós-Graduação em Proteção de Plantas do Centro de Ciências Agrárias da Universidade Federal de Alagoas.

Silva, I. O., Amorim, E. P. da R., Junior, N. A. N., Peixinho, G. S., Carnaúba, J. P., & Neto, V. F. de A. (2020a). Trichoderma spp antagonism to phytopathogens causing cassava root rot in alagoas. Revista Científica Multidisciplinar Núcleo do Conhecimento, 12(6), 149–177. https://doi.org/10.32749/nucleodoconhecimento.com.br/agronomy-en/trichoderma-antagonism

Silva, I. O., Amorim, E. P. da R., Nascimento Junior, N. A., Assunção, I. P., Carnaúba, J. P., Peixinho, G. S., Santos, M. Q. C. dos, & Araujo Neto, V. F. de. (2020b). Identificação de patógenos causadores da podridão radicular da mandioca (manihot esculenta crantz) e biocontrole de trichoderma spp sobre fusarium sp. https://doi.org/10.37423/200702008

Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729. https://doi.org/10.1093/molbev/mst197

Tuite, J. (1969). Plant pathological methods. Fungi and bacteria. Plant pathological methods. Fungi and bacteria. https://www.cabdirect.org/cabdirect/abstract/19701101886

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Published

12/09/2022

How to Cite

SILVA, I. O. .; AMORIM, E. P. da R. .; NASCIMENTO JUNIOR, N. A. .; CARNAUBA , J. P.; ARAÚJO NETO, V. F. de .; PEIXINHO, G. S. . The first report about Fusarium falciforme that causes root rot in cassava cv Rosinha, in Alagoas state, Brazil. Research, Society and Development, [S. l.], v. 11, n. 12, p. e221111234369, 2022. DOI: 10.33448/rsd-v11i12.34369. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/34369. Acesso em: 16 nov. 2024.

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Section

Agrarian and Biological Sciences