Micronucleus and FTIR spectroscopy analysis as screening tests for HPV vírus detection
DOI:
https://doi.org/10.33448/rsd-v11i16.36823Keywords:
Micronucleus; Fourier Transform Infrared Spectroscopy; HPV.Abstract
Human Papillomavirus (HPV) infection is the main precursor of cervical cancer. Pap Smear is the main diagnostic method of this neoplasm by cytological analysis, however presents a high rate of false-negative cases. This study aimed to verify if micronucleus (MN) frequency test and the Fourier Transform Infrared (FTIR) Spectroscopy analysis may be alternative useful techniques to detect HPV in cervical fluid. Fifty samples with normal cytology obtained from patients after cytopathological examination were divided into two groups based on the presence or absence of HPV by previous analysis of molecular biology. Of the fifty samples analyzed, 46% showed the presence of MN in the cells. The frequency of MN observed was higher in the samples with HPV positive and was related with age, use of oral contraceptives and use of alcoholic beverages (p <0.001). FTIR analysis spectra showed high peak DNA and proteins in samples with a high frequency of MN (1170cm-1; 1516cm-1; 1404cm-1; 1473cm-1) compared with samples with absence of MN, probably due to chromosomal histones and MN constituents. However, no statistically significant difference was observed between these groups considering that the FTIR is a screening test and not specific. Thus, by the FTIR technique it was not possible to classify the samples by the spectral differences presented. The MN test can be considered a screening test for the detection of HPV, being possible to use it in clinical practice because it is a practical, simple, low-cost and non-invasive method. However, it is important to carry out studies in larger sample groups to investigate the application of the MN test and FTIR spectroscopy in the diagnosis of this infection and in the application in the prevention of cervical cancer.
References
Adam, M. L., Pini, C., Tulio, S., Cantalice, J. C. L. L., Torres, R. A., & Correia, M. T. D. S. (2015). Assessment of the association between micronuclei and the degree of uterine lesions and viral load in women with human papillomavirus. Cancer Genomics & Proteomics, 12(2), 67-71.
Ambroise, M. M., Balasundaram, K., & Phansalkar, M. (2013). Predictive value of micronucleus count in cervical intraepithelial neoplasia and carcinoma. Turk Patoloji Derg, 29(3), 171-178.
Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a Cancer Journal for Clinicians, 68(6), 394-424.
Cassel, A. P. R., Barcellos, R. B., Silva, C. M. D. D., Almeida, S. E. D. M., & Rossetti, M. L. R. (2014). Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology. Genetics and Molecular Biology, 37, 360-363.
Conti, C., Ferraris, P., Giorgini, E., Rubini, C., Sabbatini, S., Tosi, G., ... & Valavanis, C. (2008). FT-IR microimaging spectroscopy: A comparison between healthy and neoplastic human colon tissues. Journal of Molecular Structure, 881(1-3), 46-51.
Cortés-Gutiérrez, E. I., Dávila-Rodríguez, M. I., Vargas-Villarreal, J., Hernández-Garza, F., & Cerda-Flores, R. M. (2010). Association between human papilloma virus-type infections with micronuclei frequencies. Prague Med Rep, 111(1), 35-41.
De Geus, J. L., Rezende, M., Margraf, L. S., Bortoluzzi, M. C., Fernández, E., Loguercio, A. D., ... & Kossatz, S. (2015). Evaluation of genotoxicity and efficacy of at-home bleaching in smokers: a single-blind controlled clinical trial. Operative Dentistry, 40(2), E47-E55.
Dickemann, M. C. M., Pegoraro, J., Martins, I. S., Kirsch, I., Ecker, A. C. L., Wiethölter, P., & Mozzini, C. B. (2018). Frequência de micronúcleos em etilistas. Unifunec Ciências da Saúde e Biológicas, 2(4), 1-10.
Em, P. D. P., Aires, G. M. A. (2008) Avaliação da freqüência de micronúcleos como marcador da progresão das neoplasias intraepiteliais cervicais, 71.
Franzke, B., Schober-Halper, B., Hofmann, M., Oesen, S., Tosevska, A., Nersesyan, A., ... & Wagner, K. H. (2020). Chromosomal stability in buccal cells was linked to age but not affected by exercise and nutrients-Vienna Active Ageing Study (VAAS), a randomized controlled trial. Redox biology, 28, 101362.
INCA. (2020). Estimativa 2020: Incidência de Câncer No Brasil; Ministério da Saúde: Rio de Janeiro.
Kažimírová, A., Barančoková, M., Džupinková, Z., Wsólová, L., & Dušinská, M. (2009). Micronuclei and chromosomal aberrations, important markers of ageing: possible association with XPC and XPD polymorphisms. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 661(1-2), 35-40.
Khanmohammadi, R., Mir, F., Baniebrahimi, G., & Mirzaei, H. (2018). Oral tumors in children: Diagnosis and management. Journal of Cellular Biochemistry, 119(3), 2474-2483.
Kong, J., & Yu, S. (2007). Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta biochimica et biophysica Sinica, 39(8), 549-559.
Koonmee, S., Bychkov, A., Shuangshoti, S., Bhummichitra, K., Himakhun, W., Karalak, A., & Rangdaeng, S. (2017). False-negative rate of Papanicolaou testing: A national survey from the Thai Society of Cytology. Acta Cytologica, 61(6), 434-440.
Lyng, F. M., Traynor, D., Ramos, I. R., Bonnier, F., & Byrne, H. J. (2015). Raman spectroscopy for screening and diagnosis of cervical cancer. Analytical and bioanalytical chemistry, 407(27), 8279-8289.
Melo, I. M., Ribeiro, E. A., & Canevari, R. A. (2018). Potential Diagnostic Techniques for Cervical Cancer Prevention-Review. Journal of Cancer Treatment and Diagnosis, 2(4). 10-16.
Movasaghi, Z., Rehman, S., & Ur Rehman, D. I. (2008). Fourier transform infrared (FTIR) spectroscopy of biological tissues. Applied Spectroscopy Reviews, 43(2), 134-179.
Nascimento, M. D. D. S. B., Vidal, F. C. B., Silva, M. A. C. N. D., Batista, J. E., Lacerda Barbosa, M. D. C., Muniz Filho, W. E., ... & Brito, L. M. O. (2018). Prevalence of human papillomavirus infection among women from quilombo communities in northeastern Brazil. BMC Women's Health, 18(1), 1-10.
Nefic, H., & Handzic, I. (2013). The effect of age, sex, and lifestyle factors on micronucleus frequency in peripheral blood lymphocytes of the Bosnian population. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 753(1), 1-11.
Nersesyan, A., Kundi, M., Waldherr, M., Setayesh, T., Mišík, M., Wultsch, G., ... & Knasmueller, S. (2016). Results of micronucleus assays with individuals who are occupationally and environmentally exposed to mercury, lead and cadmium. Mutation Research/Reviews in Mutation Research, 770, 119-139.
Rymsza, T., Ribeiro, E. A., Silva, L. F. D. C., Bhattacharjee, T., & de Azevedo Canevari, R. (2018). Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 196, 238-246.
Shi, Y. H., Wang, B. W., Tuokan, T., Li, Q. Z., & Zhang, Y. J. (2015). Association between micronucleus frequency and cervical intraepithelial neoplasia grade in Thinprep cytological test and its significance. International Journal of Clinical and Experimental Pathology, 8(7), 8426.
Soeteman-Hernández, L. G., Johnson, G. E., & Slob, W. (2016). Estimating the carcinogenic potency of chemicals from the in vivo micronucleus test. Mutagenesis, 31(3), 347-358.
Stich, H. F. (1987). Micronucleated exfoliated cells as indicators for genotoxic damage and as markers in chemoprevention trials. J Nutr Growth Cancer, 4(5), 9-18.
Tolbert, P. E., Shy, C. M., & Allen, J. W. (1992). Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutation Research/Environmental Mutagenesis and Related Subjects, 271(1), 69-77.
Viana, M. R. P., Melo, I. M. A., Pupin, B., Raniero, L. J., & de Azevedo Canevari, R. (2020). Molecular detection of HPV and FT-IR spectroscopy analysis in women with normal cervical cytology. Photodiagnosis and Photodynamic Therapy, 29, 101592.
Vilanova, L. B., Vianna, R. P., Bicca, G. L. O., Bicca, M. L. O., & Rocha, B. H. G. (2012). Determinação da frequência de alterações citogenéticas, através do teste de micronúcleo, em células cervicais uterinas de pacientes portadoras de HPV. VITTALLE-Revista de Ciências da Saúde, 24(1), 19-26.
Yıldırım, H., Göker, A., Demirci, H., Güvenal, T., & Korkmaz, M. (2019). A comparative study for selectivity of micronuclei in cervical exfoliated cells on chronic boron effects. Journal of Cytology, 36(2), 75-78.
Wojda, A., Ziętkiewicz, E., & Witt, M. (2007). Effects of age and gender on micronucleus and chromosome nondisjunction frequencies in centenarians and younger subjects. Mutagenesis, 22(3), 195-200.
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Copyright (c) 2022 Magda Rogéria Pereira Viana; Igor Martins Alves Melo; Nelson Jorge Carvalho Batista; Leandro José Raniero; Renata de Azevedo Canevari
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