Development of yogurts with mixture of sheep and bovine milk: effect on chemical physical characteristics, protein profile and antioxidant activity

Authors

DOI:

https://doi.org/10.33448/rsd-v11i16.37531

Keywords:

Antioxidants; Physical-chemical composition; Yogurts.

Abstract

Yogurt is one of the most popular fermented dairy products and an excellent medium for new ingredients/food source. This study sought to evaluate and characterize bovine milk (BM) and sheep milk (SM) and yogurt made from bovine (BM) and sheep (SM) milk, and BM:SM mixture with the respective 100:0 fractions: 75:25, 50:50, 25:75 and 0:100, and evaluate their influence on physicochemical parameters, antioxidant, protein profile, microstructure and color analysis for yogurts. Yogurts with higher levels of acidity, protein, fat, total solids, ash and calcium were the ones made from SM.. Regarding the color attributes, all yogurts showed yellow-green characteristics after 21 days of storage by evaluating parameters a* and b*, with a predominance of bovine milk. The antioxidant profile showed a higher concentration at 100:0 and 0:100, but bovine milk has a higher DPPH (2,2-diphenyl-1-picrylhydrazyl) value, while yogurts maintained constant DPPH values ​​during storage, being higher at 0:100. Scanning electron microscopy (SEM) showed that BM (100:0) has a more compact structure, possibly due to the larger size of the fat particles and the lower protein concentration compared to SM (0:100). Sheep milk presented better nutritional value, showing its potential in the manufacture of products.

References

Abreu, E., Preci, D., Zeni, J., Steffens, C. & Steffens, J. (2018). Development of frozen yogurt from powdered yogurt of sheep milk. Revista Ceres. 65(1): 7-15. 10.1590/0034-737X201865010002.

Abreu, E., Zeni, J., Steffens, C. & Steffens J. (2016). Frozen yogurt from sheep milk. Revista Ceres. 63(5): 605-613. 10.1590/0034-737X201663050003.

American Association of Cereal Chemists. (2000). Approved methods of the American Association of Cereal Chemists, 10th ed. St. Paul, AACC.

Asensio-Vegas, C., Tiwari, B., Gredilla, A. E., Bueno, F., Delgado, D. & Martín-Diana, A. B. (2018). Development of yoghurt from ovine milk with enhanced texture and flavour properties. International Journal of Dairy Technology. 71(1): 112-121. https://doi.org/10.1111/1471-0307.12341.

Association of Official Analitical Chemistry. (2016). Official methods of analysis of analytical chemistry. 17th. ed. AOAC.

Aryana, K. J. & Olson, D. W. (2017). A 100-year review: Yogurt and other cultured dairy products. Journal of Dairy Science. 100(12): 9987-10013. 10.3168/jds.2017-12981.

Balthazar, C. F., Pimentel, T. C., Ferrão, L. L., Almada, C. N., Santillo, A., Albenzio, M., Mollakhalili, N., Mortazavian, A. M., Nascimento, J. S., Silva, M. C., Freitas, M. Q., Sant’ana, A. S., Granato, D. & Cruz, A. G. (2017). Sheep milk: Physicochemical characteristics and relevance for functional food development. Comprehensive Reviews in Food Science and Food Safety. 16(2): 247-262. https://doi.org/10.1111/1541-4337.12250.

Balthazar, C. F., Santillo, A., Figliola, L., Silva, H. L. A., Esmerino, E. A., Freitas, M. Q. & Albenzio, M. (2018). Avaliação sensorial de uma bebida de morango com leite de ovelha prebiótico. LWT – Food Science and Technology. 98: 94-98. 10.1016/j.lwt.2018.08.017.

Bett, S. C., Picanço, N. F. M., De Faria, R. A. P. G. & Nascimento, E. (2017). Elaboração e qualidade de iogurte com polpa de mangaba. Higiene Alimentar. 31(272/273): 120-127.

Bramanti, E., Sortino, C., Onor, M., Beni, F. & Raspi, G. (2003). Separation and determination of denatured as1-, as2-, b- and k-caseins by hydrophobic interaction 50 chromatography in cows’, ewes’ and goats’ milk, milk mixtures and cheeses. Journal of Chromatography A. 994(1-2): 59-74. 10.1039/b102360h.

Brand-Williams, W., Cuvelier, M. E. & Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. LWT – Food Science and Technology. 28(1): 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5.

Brasil. (2007). Instrução Normativa Nº 46 de 23 de outubro de 2007. Regulamento técnico de identidade e qualidade de leites fermentados. Ministério da Agricultura, Pecuária e Abastecimento.

Caleja, C., Barros, L., Antonio, A.L., Carocho, M, Oliveira, M.B., & Ferreira, I.C.F.R. (2016). Fortification of yogurts with different antioxidant preservatives: A comparative study between natural and synthetic additives. Food Chemistry, 210, 262– 268. 10.1016/j.foodchem.2016.04.114.

Costa, M. P., Balthazar, C. F., Rodrigues, B. L., Lazaro, C. A., Silva, A. C. O., Cruz, A. G. & Conte Junior, C. A. (2015). Determination of biogenic amines by high-performance liquid chromatography (HPLC-DAD) in probiotic cow’s and goat’s fermented milks and acceptance. Food Science & Nutrition. 3(3): 172-178. 10.1002/fsn3.200.

Costa, M. P., Monteiro, M. L. G., Frasao, B. S., Silva, V. L. M., Rodrigues, B. L., Chiappini, C. C. J. & Conte-Junior, C. A. (2017). Consumer perception, health information, and instrumental parameters of cupuassu (Theobroma grandiflorum) goat milk yogurts. Journal of Dairy Science. 100(1): 157-168. https://doi.org/10.3168/jds.2016-11315.

Cottica, S. M., Amado, D. A. V., Aguiar, S. C. de, Boeing, J. S., Franco, S. L., Zeoula, L. M. & Visentainer, J. V. (2018). Antioxidant activity and lipid oxidation in milk from cows with soybean oil and propolis extract added to their feed. Food Science and Tecnology. 39(2): 1-8. https://doi.org/10.1590/fst.33817.

De La Vara, J. A., Berruga, M. I., Cappelli, J., Landete-Castillejos, T., Carmona, M., Gallego, L. & Molina, A. (2018). Some aspects of the ethanol stability of red deer milk (Cervus elaphus hispanicus): A comparison with other dairy species. International Dairy Journal. 86. 103-109. https://doi.org/10.1016/j.idairyj.2018.07.006.

Dias, R. S., Balthazar, C. F., Cavalcanti, R. N., Sobral, L. A., Rodrigues, J. F., Neto, R. P. C., Tavares, M. I. B., Ribeiro, A. P. B., Grimaldi, R., Sant'Anna, C., Esmerino, E. A., Pimentel, T. C., Silva, M. C., Cruz, A. G. (2022). Nutritional, rheological and sensory properties of butter processed with different mixtures of cow and sheep milk cream. Food Bioscience. 46: 1-11. https://doi.org/10.1016/j.fbio.2022.101564.

Eissa, E. A., Barbiker, E. E., Yagoub, A. E. A. (2011). Physicochemical, microbiological and sensory properties of Sudanese yoghurt (zabadi) made from goat’s milk. Animal Production Science. 51(1): 53-59. 10.1071/AN09126.

Farrell, H. M., Malin, E. L., Brown, E. M. & Qi, P. X. (2006). Casein micelle estructure: What can be learned from milk synthesis and structural biology? Current Opinion in Colloid & Interface Science. 11(1-2): 135-147. https://doi.org/10.1016/j.cocis.2005.11.005.

Farvin, K. H. S., Baron, C. P., Nielsen, N. S. & Jacobsen, C. (2010). Antioxidant activity of yoghurt peptides: Part 1-in vitro assays and evaluation in ω-3 enriched milk. Food Chemistry. 123: 1081-1089. 10.1016/j.foodchem.2010.05.067.

Fava, L. W., Külkamp-Guerreiro, C. & Pinto, A. T. (2014). Evaluation of physico-chemical characteristics of fresh, refrigerated and frozen Lacaune ewes’ milk. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 66(6): 1924-1930. https://doi.org/10.1590/1678-7675.

Fazilah, N. F., Ariff, A. B., Khayat, M. E., Rios-Solis, L. & Halim, M. (2018). Influence of probiotics, prebiotics, synbiotics and bioactive phytochemicals on the formulation of functional yogurt. Journal of Functional Foods. 48: 387-399. https://doi.org/10.1016/j.jff.2018.07.039.

Feng, C., Wang, B., Zhao, A., Weu, L., Shao, Y., Wang, Y., Cao, Z. & Zhang, F. (2019). Quality characteristics and antioxidant activities of goat milk yogurt with added jujube pulp. Food Chemistry. 277: 238-245. 10.1016/j.foodchem.2018.10.104.

Gagnon, N., Côrtes, C., da Silva, D., Kazama, R., Benchaar, C., dos Santos, G., Zeoula, L. & Petit, H. V. (2009). Ruminal metabolism of flaxseed (Linum usitatissimum) lignans to the mammalian lignan enterolactone and its concentration in ruminal fluid, plasma, urine and milk of dairy cows. The British Journal of Nutrition. 102(7): 1015-1023. 10.1017/S0007114509344104.

Gantner, V., Mijic, P., Baban, M., Zoran, S. & Alka, T. (2015). The overall and fat composition of milk of various species. Mljekarstvo. 65(4): 223-231. 10.15567/mljekarstvo.2015.0401.

García-Pérez, F. J., Lario, Y., Fernández-López, J., Sayas, E., Pérez Alvarez, J. A. & Sendra, E. (2005). Effect of orange fiber addition on yogurt color during fermentation and cold storage. Industrial Applications. 30(6): 457-463. https://doi.org/10.1002/col.20158.

Gazzani, G., Papti, A., Massolini, G. & Daglia, M. (1998). Atividade anti e pró-oxidante de componentes solúveis em água de alguns vegetais comuns da dieta e o efeito do tratamento térmico. Journal of Agricultural and Food Chemistry. 46(10): 4118-4122. 10.1021/jf980300o.

Gomes, J. J. L., Duarte, A. M., Batista, A. S. M., de Figueiredo, R. M. F., de Sousa, E. P., de Souza, E. L. & do Egypto, R. D. C. R. (2013). Physicochemical and sensory properties of fermented dairy beverages made with goat's milk, cow's milk and a mixture of the two milks. LWT – Food Science and Technology. 54(1): 18-24. https://doi.org/10.1016/j.lwt.2013.04.022.

Instituto Adolfo Lutz. (2008). Métodos físico-químicos para análise de alimentos. Instituto Adolfo Lutz.

Jørgensen, C. E., Abrahamsen, R. K., Rukke, E. O., Hoffmann, T. K., Johansen, A. G. & Skeie, S. B. (2019). Processing of high-protein yoghurt – A review. International Dairy Journal. 88: 42-59. https://doi.org/10.1016/j.idairyj.2018.08.002.

Kalyankar, S. D., Sarode, A. R., Khedkar, C. D., Deosarkar, S. S. & Pawshe, R. D. (2016). Sheep: Milk. In: Encyclopedia of Foods & Health. Elsevier Publishers.

Kaminarides, S., Stamou, P. & Massouras, T. (2007). Comparison of the characteristics of set type yoghurt made from ovine milk of different fat content. International Journal of Food Science & Technology. 42(9): 1019-1028. https://doi.org/10.1111/j.1365-2621.2006.01320.x.

Karaaslan, M., Ozden, M., Vardin, H. & Turkoglu, H. (2011). Phenolic fortification of yogurt using grape and callus extracts. LWT – Food Science and Technology. 44(4): 1065-1072. https://doi.org/10.1016/j.lwt.2010.12.009.

Karnopp, A. R., Oliveira, K. G., Andrade, E. F., Postingher, B. M. & Granato, D. (2017). Optimization of an organic yogurt based on sensorial, nutritional, and functional perspectives. Food Chemistry. 233: 401-411. https://doi.org/10.1016/j.foodchem.2017.04.112.

Konuspayeva, G.S. (2020). Camel milk composition and nutritional value O. Alhaj, B. Faye, R. Agrawal (Eds.), Handbook of research on Health and Environmental Benefits of camel products, IGI Global, Hershey, pp. 15-40. 10.4018/978-1-7998-1604-1.ch002

Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685. https://doi.org/10.1038/227680a0.

Lima, M S. F, Silva, R. A., Silva, M. F., Porto, A. L. F., Cavalcanti, M. T. H.. (2014). Características microbiológicas e antioxidantes de um novo alimento funcional probiótico: leite de ovelha fermentado por kefir. In: XX Congresso Brasileiro de Engenharia Quimica, 20. Florianópolis. Anais Florianópolis-SC.

Lima, P. C., Marco, I., Stein, V., Pagotto, C. K., Rigo, E., Cavalheiro, D., Schogor, A. L. B. (2020). Obtaining and characterizing dulce de leche prepared with sheep’s and cow’s milk in different proportions. Food Sci. Technol (Campinas), v. 40, p. 832-837, 2020. https://doi.org/10.1590/fst.16819

Magenis, R. B., Prudêncio, E. S., Fritzen-Freire, C. B., Stephan, M. P., Egito, A. S. & Daguer, H. (2014). Rheological, physicochemical and authenticity assessment of Minas Frescal cheese. Food Control. 45: 22-28. https://doi.org/10.1016/j.foodcont.2014.04.012.

Malta, D S, Dalmina, E M, Silva, F C, Schmeier, M N, Siviero, B, Bianchi, A E, Cavalheiro, D, Rigo, E. (2021). Physicochemical and microbiological characteristics, proteolytic, and lipolytic activities of sheep milk produced in different seasons. Brazilian Journal of Development. https://doi.org/10.34117/bjdv7n12-790

Masoodi, T. A. & Shafi, G. (2010). Analysis of casein alpha S1 & S2 proteins from different mammalian species. Biomedical Informatics. 4(9): 430-435. 10.6026/97320630004430.

Mior, J., Novello, Z. & Dinon, A. Z. (2016). Characterization of sheep’s milk yoghurt in natura and flavored with blueberry. Revista Brasileira de Tecnologia Agroindustrial. 10(1): 2004-2022. 10.3895/rbta.v10n1.1984.

Mohammadi-Gouraji, E., Soleimanian-Sad, S. & Ghiaci, M. (2019). Phycocyanin-enriched yogurt and its antibacterial and physicochemical properties during 21 days of storage. LWT – Food Science and Technology. 102: 230-236. 10.1016/j.lwt.2018.09.057.

Nguyen, H. T. H., Afsar, S. & Day, L. (2018). Differences in the microstructure and rheological properties of low-fat yoghurts from goat, sheep and cow milk. Food Research International. 108: 423-429. 10.1016/j.foodres.2018.03.040.

Nguyen, H. T. H., Ong, L., Lefèvre, C., Kentish, S. E. & Gras, S. L. (2013). The microstructure and physicochemical properties of buffalo probiotic yogurt during fermentation and storage: a comparison with bovine yogurt. Food and Bioprocess Technology. 7(4): 937-953. 10.1007/s11947-013-1082-z.

Pandya, A. J. & Ghodke, K. M. (2007). Goat and sheep milk products other than cheeses and yoghurt. Small Ruminant Research. 68(1-2): 193-206. https://doi.org/10.1016/j.smallrumres.2006.09.007.

Park, Y. W., Juárez, M., Ramos, M. & Haenlein, G. F. W. (2007). Physico-chemical characteristics of goat and sheep milk. Small Ruminants Reseasrch. 68(1-2): 88-113. https://doi.org/10.1016/j.smallrumres.2006.09.013.

Payandeh, S., Kafilzadeh, F., De La Fuente, M. A., Ghadimi, D. & Marín, A. L. M. (2015). Patterns of milk production, blood metabolite profile and enzyme activities of two fat-tailed sheep breeds. Animal Production Science. 56(9): 1469-1474. https://doi.org/10.1071/AN141035.

Peixoto, A. L., Silva, M. A. P., Moura, L. C., Souza, D. G., Plácido, G. R. & Caliari, M. (2016). Influence of time of storage of milk in physical and chemical characteristics of natural yogurt. Revista Brasileira de Produtos Agroindustriais. 18(4): 395-403. 10.15871/1517-8595/rbpa.v18n4p395-403.

Pereira da Costa, M. & Conte-Junior, C. A. (2015). Chromatographic Methods for the Determination of Carbohydrates and Organic Acids in Foods of Animal Origin. Comprehensive Reviews in Food Science and Food Safety. 14(5): 586-600. https://doi.org/10.1111/1541-4337.12148.

Preci, D., Fernandes, I. A., Valduga, E., Cansian, R. L., Steffens, J., Steffens, C., Brião, V. B. (2021). Hydrolysis of sheep's milk whey concentrate by membrane and evaluation of the antioxidant and antimicrobial properties. Scientia Plena, 17 (2): 021501. 10.14808/sci.plena.2021.021501

Rashidi, L., Gholami, Z., Nanvazadeh, S. & Shabani, Z. (2016). Rapid method for extracting and quantifying synthetic antioxidants in all edible fats and oils. Food Analytical Methods. 9(9): 2682-2690. https://doi.org/10.1007/s12161-016-0443-4.

Rettedal, E. A., Altermann, E., Roy, N. C. & Dalziel, J. E. (2019). The effects of unfermented and fermented cow and sheep milk on the gut microbiota. Frontiers in Microbiology. 10: 1-12. https://doi.org/10.3389/fmicb.2019.00458.

Revers, L. M., Danielli, A. J., Iltchenco, S., Zeni, J., Steffens, C. & Steffens, J. (2016). Obtenção e caracterização de iogurtes elaborados com leites de ovelha e de vaca. Revista Ceres. 63(6): 747-753. https://doi.org/10.1590/0034-737X201663060001.

Roy, D., Ye, A., Moughan, P. J. & Singh, H. (2020). Composition, Structure, and Digestive Dynamics of Milk From Different Species – A Review. Frontiers in Nutrition. 7: 1-17. https://doi.org/10.3389/fnut.2020.577759.

Selvaggi, M., Laudadio, V., Dario, C. & Tufarelli, V. (2014). Investigating the genetic polymorphism of sheep milk proteins: a useful tool for dairy production. Journal of the Science of Food and Agriculture. 94(15): 3090-3099. 10.1002/jsfa.6750.

Serra, M., Trujillo, A. J., Guamis, B. & Ferragut, V. (2009). Proteolysis of yogurts made from ultra-high pressure homogenized milk during cold storage. Journal of Dairy Science. 92(1): 71-78. https://doi.org/10.3168/jds.2008-1416.

Sultan, S., Huma, N., Butt, M. S. & Shahid, M. (2016). Antihypertensive and Antioxidative Potential of Water Soluble Peptide Fraction from Different Yoghurts. Journal of Food Processing and Preservation. 41(3): 1-7. https://doi.org/10.1111/jfpp.12979.

Tong, L. M., Sasaki, S., McClements, D. J. & Decker, E. A. (2000). Antioxidant activity of whey in a salmon oil emulsion. Journal of Food Science. 65(8): 1325-1329. https://doi.org/10.1111/j.1365-2621.2000.tb10606.x.

Tribst, A. A. L., Falcade, L. T. P., Carvalho, N. S., Leite Junior, B. R. de C. & Oliveira, M. M. de. (2020a). Are stirring and homogenisation processes capable of improving physicochemical and sensory characteristics of stirred yoghurt produced with fresh, refrigerated and frozen/thawed sheep milk? International Dairy Journal. 109: 1-8. https://doi.org/10.1016/j.idairyj.2020.104778.

Tribst, A. A. L., Falcade, L. T. P., Carvalho, N. S., Leite Junior, B. R. de C., Oliveira, M. M. de. (2020b). Using stirring and homogenization to improve the fermentation profile and physicochemical characteristics of set yogurt from fresh, refrigerated and frozen/thawed sheep milk. LWT – Food Science and Technology. 130: 1-9. https://doi.org/10.1016/j.lwt.2020.109557.

Tribst, A. A. L., Falcade, L. T. P. & Oliveira, M. M. D. (2019). Strategies for raw sheep milk 524 storage in smallholdings: Effect of freezing or long-term refrigerated storage on microbial 525 growth. Journal of Dairy Science. 102(6): 4960-4971. 10.3168/jds.2018-15715.

Tribst, A. A. L., Ribeiro, L. R., Leite Junior, B. R. D. C., de Oliveira, M. M. & Cristianini, M. (2018). Fermentation profile and characteristics of yoghurt manufactured from frozen sheep milk. International Dairy Journal, 78: 36-45. https://doi.org/10.1016/j.idairyj.2017.10.005.

Vianna, F. S., Canto, A. C. V. C. S., Da Costa-Lima, B. R. C., Salim, A. P. A. A., Costa, M. P., Balthazar, C. F. & Silva, A. C. O. (2017). Development of new probiotic yoghurt with a mixture of cow and sheep milk: effects on physicochemical, textural and sensory analysis. Small Ruminant Research. 149: 154-162. https://doi.org/10.1016/j.smallrumres.2017.02.013.

Zamberlin, Š. & Samaržija, D. (2017). The effect of non-standard heat treatment of 543 sheep’s milk on physico-chemical properties, sensory characteristics, and the bacterial 544 viability of classical and probiotic yogurt. Food Chemistry. 225: 62-68. 10.1016/j.foodchem.2017.01.001.

Wang, B. N., Liu, H. F., Zheng, J. B., Fun, M. T., Cao, W. (2011). Distribuition of phenolic acids in different tissues of jujube and their antioxidant activity. Journal of Agricultural and Food Chemistry. 59(4): 1288-1292. https://doi.org/10.1021/jf103982q.

Wang, T. & Lee, H. G. (2015). Advances in research on cis-9, trans-11 conjugated linoleic acid: A major functional conjugated linoleic acid isomer. Critical Review in Food Science and Nutrition. 55(5): 720-731. 10.1080/10408398.2012.674071.

Yuan, G. F., Chen, X. E. & Li, D. (2014). Conjugated linolenic acids and their bioactivities: A review. Food & Function. 5(7): 1360-1368. 10.1039/c4fo00037d.

Yuksel, Z., Avci, E., Uymaz, B. & Erdem, Y. K. (2012). General composition and protein surface hydrophobicity of goat, sheep and cow milk in the region of Mount Ida. Small Ruminant Research. 106(2-3): 137-144. https://doi.org/10.1016/j.smallrumres.2012.03.022.

Yuliarti, O., Mei, K. H., Ting, Z. K. X. & Yi, K. Y. (2019). Influence of combination carboxymethylcellulose and pectin on the stability of acidified milk drinks. Food Hydrocolloids. 89: 216-223. https://doi.org/10.1016/j.foodhyd.2018.10.040.

Downloads

Published

05/12/2022

How to Cite

TRENTIN, M. M.; BECKER, A. F. .; HANAUER , D. .; BIANCHI , A. .; BORGES , L. .; SCHOGOR , A. L. B.; RIGO , E. Development of yogurts with mixture of sheep and bovine milk: effect on chemical physical characteristics, protein profile and antioxidant activity. Research, Society and Development, [S. l.], v. 11, n. 16, p. e192111637531, 2022. DOI: 10.33448/rsd-v11i16.37531. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/37531. Acesso em: 26 apr. 2024.

Issue

Section

Agrarian and Biological Sciences