Ultradiluted Plumbum metallicum in reversal of neurotoxicity caused by lead in Danio rerio
DOI:
https://doi.org/10.33448/rsd-v11i15.37570Keywords:
Homeopathy; Zebrafish; Heavy metals; Lead; Anxiety; Neurotoxicity.Abstract
Environmental pollution caused by heavy metal residues, which are endocrine disruptors, is very relevant due to its wide use in industrial and agricultural processes. Lead is a heavy metal that is highly toxic to the environment and organisms such as fish and aquatic invertebrates. This work aims to evaluate the behavioral changes in zebrafish (Danio rerio) induced by Lead, through the analyzes of locomotor activity and anxiety parameters, verifying if homeopathic medicines are able to reduce the neurotoxic effects caused in the fish organism. The Danio rerio fishes were kept in maintenance aquariums, with controlled parameters, until the time of the experiments. The animals were exposed to heavy metal and the groups were composed of 8 animals each: white control, medication at 6cH, 30cH and inert solution, the experiment was blinded. After that, the animals underwent behavioral tests (locomotion and anxiety) to assess neurotoxicity. Lead caused anxiety in animals submitted to it with no medication (inert solution) since the time spent on the bright side of the aquarium was shorter in these animals, also reducing the distance traveled by the animals on the bright side. In the open field, the decrease in quadrants traveled by the animals that received lead without exposure to the medication is notable compared to the white control group. The group that took Plumbum metallicum 6cH had their locomotor conditions reversed, whereas the group that took Plumbum metallicum 30cH had their neurotoxicological conditions reversed.
References
Berlanda, A., Baum, C. A., Becegato, V. A., & Souza, N. C. (2021). Avaliação temporal e espacial da qualidade das águas superficiais da bacia hidrográfica do rio Desquite, Santa Catarina. Engenharia Sanitaria e Ambiental.
Blaser, R. E., Chadwick, L., & McGinnis, G. C. (2010). Behavioral measures of anxiety in zebrafish (Danio rerio). Behavioural brain research, 208(1), 56–62. https://doi.org/10.1016/j.bbr.2009.11.009
Charkiewicz, A. E., & Backstrand, J. R. (2020). Lead Toxicity and Pollution in Poland. International journal of environmental research and public health, 17(12), 4385. https://doi.org/10.3390/ijerph17124385
Costa, E. M. F., Spritzer, P. M., Hohl, A., & Bachega, T. A. S. S. (2014). Effects of endocrine disruptors in the development of the female reproductive tract. Arquivos Brasileiros de Endocrinologia & Metabologia, 58(2), 153–161. doi:10.1590/0004-2730000003031
Dutra, L.S., Ferreira, A.P., Horta, M.A., & Palhares, P.R. (2020). Uso de agrotóxicos e mortalidade por câncer em regiões de monoculturas. Saúde em Debate., 44(127), 1018-1035.
da Fonseca, J. J. S. (2002). Apostila de metodologia da pesquisa científica..Fortaleza: UEC.
Jaishankar, M., Tseten, T., Anbalagan, N., Mathew, B. B., & Beeregowda, K. N. (2014). Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary Toxicology, 7(2), 60–72. doi:10.2478/intox-2014-0009
Kavlock, R. J., Daston, G. P., DeRosa, C., Fenner-Crisp, P., Gray, L. E., Kaattari, S., & Tilson, H. A. (1996). Research needs for the risk assessment of health and environmental effects of endocrine disruptors: a report of the U.S. EPAsponsored workshop. Environmental Health Perspectives, 104(suppl 4), 715– 740.
Lee, J. W., Choi, H., Hwang, U. K., Kang, J. C., Kang, Y. J., Kim, K. I., & Kim, J. H. (2019). Toxic effects of lead exposure on bioaccumulation, oxidative stress, neurotoxicity, and immune responses in fish: A review. Environmental toxicology and pharmacology, 68, 101–108. https://doi.org/10.1016/j.etap.2019.03.010
Lindgren, K. N., Ford, D. P., & Bleecker, M. L. (2003). Pattern of blood lead levels over working lifetime and neuropsychological performance. Archives of environmental health, 58 6, 373-9.
Markowitz, M. (2000). Lead Poisoning. Pediatr Rev 21(10): 327–335.
Mason, L. H., Harp, J. P., & Han, D. Y. (2014). Pb neurotoxicity: neuropsychological effects of lead toxicity. BioMed research international, 2014, 840547. https://doi.org/10.1155/2014/840547
Moreira, H. M., Leite, L. B., Almeida, A. A., Biagini, M., et al. Emprego de Plumbum metallicum 15 cH no Saturnismo Ocupacional, Homeopat. Bras., 9(1), 33-36, 2003.
Patocka, J., & Cerný, K. (2003). Inorganic lead toxicology. Acta medica (Hradec Kralove), 46(2), 65–72.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica [Scientific Research Methodology] (e-book). Santa Maria Ed., UAB/NTE/UFSM.
Ravipati, E. S., Mahajan, N. N., Sharma, S., Hatware, K. V., & Patil, K. (2021). The toxicological effects of lead and its analytical trends: an update from 2000 to 2018. Critical reviews in analytical chemistry, 51(1), 87–102. https://doi.org/10.1080/10408347.2019.1678381
Sarath, N. G., & Puthur, J. T. (2021). Heavy metal pollution assessment in a mangrove ecosystem scheduled as a community reserve. Wetlands Ecol Manage, 29, 719–730.
Senger, M. R., Rico, E. P., de Bem Arizi, M., Frazzon, A. P., Dias, R. D., Bogo, M. R., & Bonan, C. D. (2006). Exposure to Hg2+ and Pb2+ changes NTPDase and ecto-5'-nucleotidase activities in central nervous system of zebrafish (Danio rerio). Toxicology, 226 2-3, 229-37.
Siebel, A. M., Bonan, C. D., & Silva, R. S. Zebrafish como modelo para estudos comportamentais. Citado por Resende, R.R.; Soccol, C.R. Biotecnologia aplicada à saúde: fundamentos e aplicações. Volume 1. São Paulo. Blucher. 2015.
Silveira, D. T., & Córdova, F. P. (2009). A pesquisa cientítica. Métodos de pesquisa. Porto Alegre: Editora da UFRGS, 2009. p. 33-44.
Signorini, A., Lubrano, A., Manuele, G., Fagone, G., Vittorini, C., Boso, F., Vianello, P., Rebuffi, A., Frongia, T., Rocco, V., & Pichler, C. (2005). Classical and new proving methodology: provings of Plumbum metallicum and Piper methysticum and comparison with a classical proving of Plumbum metallicum. Homeopathy: the journal of the Faculty of Homeopathy, 94(3), 164–174. https://doi.org/10.1016/j.homp.2005.02.009
Stewart, W. F., Schwartz, B. S., Davatzikos, C., Shen, D., Liu, D., Wu, X., Todd, A. C., Shi, W., Bassett, S., & Youssem, D. (2006). Past adult lead exposure is linked to neurodegeneration measured by brain MRI. Neurology, 66(10), 1476–1484. https://doi.org/10.1212/01.wnl.0000216138.69777.15
Trigueiro, N. S. S., Canedo, A., Braga, D. L. S., Luchiari, A. C., & Rocha, T. L. (2020). Zebrafish as an emerging model system in the global south: two decades of research in brazil. Zebrafish, 17(6), 412–425. https://doi.org/10.1089/zeb.2020.1930
Yuan, Y., Wu, Y., Ge, X., Nie, D., Wang, M., Zhou, H., & Chen, M. (2019). In vitro toxicity evaluation of heavy metals in urban air particulate matter on human lung epithelial cells. The Science of the total environment, 678, 301–308. https://doi.org/10.1016/j.scitotenv.2019.04.431.
Zar, J. H. (2010) Biostatistical Analysis. 5th Edition, Prentice-Hall/Pearson, Upper Saddle River, xiii, 944 p.
Zhang, Y., Zhang, P., Shang, X., Lu, Y., & Li, Y. (2021). Exposure of lead on intestinal structural integrity and the diversity of gut microbiota of common carp. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 239, 108877. https://doi.org/10.1016/j.cbpc.2020.108877
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Copyright (c) 2022 Henrique Carvallo Vieira; Katia Lima Traldi Cappelli; Rafael Acordi dos Santos; Marco Aurélio Gonçalves Manzoli; Melina Castilho de Souza Balbueno; Cidéli de Paula Coelho
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