Intermittent fasting associated with coffee improves lipid profile and hepatic steatosis in hyperlipidemic rats
DOI:
https://doi.org/10.33448/rsd-v11i16.38061Keywords:
Fasting; Obesity; Coffee; Cholesterol; HDL; Metabolic syndrome.Abstract
The aim of this study was to evaluate the association of intermittent fasting (IF) and coffee intake in Wistar rats pre-treated with a high-fat diet. Newborn Wistar rats were used, randomly divided into 4 groups: Control (CO, commercial feed), Control+Coffee (CA, commercial feed+coffee), Fasting (IF, 24 hours feeding/24 hours fasting), Fasting+Coffee (IFCA, 24 hours feeding/24 hours fasting+coffee). The animals were pre-treated with a hyperlipidic diet for 30 days. The coffee administered to the animals was 100% Arabica, at a dose of 0.5 mL by gavage daily. Weight was measured weekly throughout the experiment and after euthanasia, subcutaneous and visceral fat were removed and weighed. Glucose and insulin rates, lipid profile and liver histology were analyzed. The fasting groups had significantly lower results in weight and fat mass gain, better lipid profile and lower hepatic fat deposition. Lower collagen deposition was observed in the groups that ingested coffee, as well as a lower moisture content. From the results obtained, it can be concluded that the combination of interventions can be beneficial in reducing weight gain and body fat, in addition to having a synergistic effect in reducing hepatic steatosis in rats pre-treated with a high-fat diet.
References
AOAC, I. (2016). AOAC: Official Methods of Analysis (Vol. 1).
Blüher M. (2019). Obesity: global epidemiology and pathogenesis. Nature reviews. Endocrinology, 15(5), 288–298. https://doi.org/10.1038/s41574-019-0176-8
Chaix, A., Lin, T., Le, H. D., Chang, M. W., & Panda, S. (2019). Time-Restricted Feeding Prevents Obesity and Metabolic Syndrome in Mice Lacking a Circadian Clock. Cell metabolism, 29(2), 303–319.e4. https://doi.org/10.1016/j.cmet.2018.08.004
Cho, A. S., Jeon, S. M., Kim, M. J., Yeo, J., Seo, K. I., Choi, M. S., & Lee, M. K. (2010). Chlorogenic acid exhibits anti-obesity property and improves lipid metabolism in high-fat diet-induced-obese mice. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association, 48(3), 937–943. https://doi.org/10.1016/j.fct.2010.01.003
Cobas R, Rodacki M, Giacaglia L, Calliari L, Noronha R, Valerio C, Custódio J, Santos R, Zajdenverg L, Gabbay G, Bercoluci M. (2022). Diagnóstico do diabetes e rastreamento do diabetes tipo 2. Diretriz Oficial da Sociedade Brasileira de Diabetes. DOI: 10.29327/557753.2022-2, ISBN: 978-65-5941-622-6.
Ferrão, R. G., Fonseca, A. F. A. da, Ferrão, M. A. G., & De Muner, L. H. (2019). Coffea canephora. In R. G. Ferrão, A. F. A. da Fonseca, M. A. G. Ferrão, & L. H. De Muner (Eds.), Conilon coffee. Vitória, ES: Incaper.
Ghanbari, M., Momen Maragheh, S., Aghazadeh, A., Mehrjuyan, S. R., Hussen, B. M., Abdoli Shadbad, M., Dastmalchi, N., & Safaralizadeh, R. (2021). Interleukin-1 in obesity-related low-grade inflammation: From molecular mechanisms to therapeutic strategies. International immunopharmacology, 96, 107765. https://doi.org/10.1016/j.intimp.2021.107765
Gotthardt, J. D., Verpeut, J. L., Yeomans, B. L., Yang, J. A., Yasrebi, A., Roepke, T. A., & Bello, N. T. (2016). Intermittent Fasting Promotes Fat Loss With Lean Mass Retention, Increased Hypothalamic Norepinephrine Content, and Increased Neuropeptide Y Gene Expression in Diet-Induced Obese Male Mice. Endocrinology, 157(2), 679–691. https://doi.org/10.1210/en.2015-1622
Harvie, M., & Howell, A. (2017). Potential Benefits and Harms of Intermittent Energy Restriction and Intermittent Fasting Amongst Obese, Overweight and Normal Weight Subjects-A Narrative Review of Human and Animal Evidence. Behavioral sciences (Basel, Switzerland), 7(1), 4. https://doi.org/10.3390/bs7010004
Horne, B. D., Muhlestein, J. B., Lappé, D. L., May, H. T., Carlquist, J. F., Galenko, O., Brunisholz, K. D., & Anderson, J. L. (2013). Randomized cross-over trial of short-term water-only fasting: metabolic and cardiovascular consequences. Nutrition, metabolism, and cardiovascular diseases: NMCD, 23(11), 1050–1057. https://doi.org/10.1016/j.numecd.2012.09.007
Laurens, C., Grundler, F., Damiot, A., Chery, I., Le Maho, A. L., Zahariev, A., Le Maho, Y., Bergouignan, A., Gauquelin-Koch, G., Simon, C., Blanc, S., & Wilhelmi de Toledo, F. (2021). Is muscle and protein loss relevant in long-term fasting in healthy men? A prospective trial on physiological adaptations. Journal of cachexia, sarcopenia and muscle, 12(6), 1690–1703. https://doi.org/10.1002/jcsm.12766
Lee, A., Lim, W., Kim, S., Khil, H., Cheon, E., An, S., Hong, S., Lee, D. H., Kang, S. S., Oh, H., Keum, N., & Hsieh, C. C. (2019). Coffee Intake and Obesity: A Meta-Analysis. Nutrients, 11(6), 1274. https://doi.org/10.3390/nu11061274
Lemos, M. F., de Andrade Salustriano, N., de Souza Costa, M. M., Lirio, K., da Fonseca, A. F. A., Pacheco, H. P., Endringer, D. C., et al. (2022). Chlorogenic acid and caffeine contents and anti-inflammatory and antioxidant activities of green beans of conilon and arabica coffees harvested with different degrees of maturation. Journal of Saudi Chemical Society, 26(3), 101467. https://doi.org/10.1016/j.jscs.2022.101467
López-Cruz, L., Salamone, J. D., & Correa, M. (2018). Caffeine and Selective Adenosine Receptor Antagonists as New Therapeutic Tools for the Motivational Symptoms of Depression. Frontiers in pharmacology, 9, 526. https://doi.org/10.3389/fphar.2018.00526
Lund, J., Hafstad, A. D., Boardman, N. T., Rossvoll, L., Rolim, N. P., Ahmed, M. S., Florholmen, G., Attramadal, H., Wisløff, U., Larsen, T. S., & Aasum, E. (2015). Exercise training promotes cardioprotection through oxygen-sparing action in high fat-fed mice. American journal of physiology. Heart and circulatory physiology, 308(8), H823–H829. https://doi.org/10.1152/ajpheart.00734.2014
Ma, Y., Gao, M., & Liu, D. (2015). Chlorogenic acid improves high fat diet-induced hepatic steatosis and insulin resistance in mice. Pharmaceutical research, 32(4), 1200–1209. https://doi.org/10.1007/s11095-014-1526-9
Pan, M. H., Tung, Y. C., Yang, G., Li, S., & Ho, C. T. (2016). Molecular mechanisms of the anti-obesity effect of bioactive compounds in tea and coffee. Food & function, 7(11), 4481–4491. https://doi.org/10.1039/c6fo01168c
Pimentel, G. D., Micheletti, T. O., Fernandes, R. C., Nehlig A. (2019). Coffee Intake and obesity. Nutrition in the Prevention and Treatment of Abdominal Obesity. Elsevier; pp. 329-51. https://doi.org/10.1016/B978-0-12-816093-0.00024-0
Pimpley, V., Patil, S., Srinivasan, K., Desai, N., & Murthy, P. S. (2020). The chemistry of chlorogenic acid from green coffee and its role in attenuation of obesity and diabetes. Preparative biochemistry & biotechnology, 50(10), 969–978. https://doi.org/10.1080/10826068.2020.1786699
Shin, J. W., Wang, J. H., Kang, J. K., & Son, C. G. (2010). Experimental evidence for the protective effects of coffee against liver fibrosis in SD rats. Journal of the science of food and agriculture, 90(3), 450–455. https://doi.org/10.1002/jsfa.3838
Sirotkin, A. V., & Kolesárová, A. (2021). The anti-obesity and health-promoting effects of tea and coffee. Physiological research, 70(2), 161–168. https://doi.org/10.33549/physiolres.934674
Strasser, B., Spreitzer, A., & Haber, P. (2007). Fat loss depends on energy deficit only, independently of the method for weight loss. Annals of nutrition & metabolism, 51(5), 428–432. https://doi.org/10.1159/000111162
WHO. (2021, June 9). Obesity and overweight. Retrieved October 23, 2022, from https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight
Williamson, E., & Moore, D. R. (2021). A Muscle-Centric Perspective on Intermittent Fasting: A Suboptimal Dietary Strategy for Supporting Muscle Protein Remodeling and Muscle Mass? Frontiers in nutrition, 8, 640621. https://doi.org/10.3389/fnut.2021.640621
Wilson, R. A., Deasy, W., Stathis, C. G., Hayes, A., & Cooke, M. B. (2018). Intermittent Fasting with or without Exercise Prevents Weight Gain and Improves Lipids in Diet-Induced Obese Mice. Nutrients, 10(3), 346. https://doi.org/10.3390/nu10030346
Yin, C., Li, Z., Xiang, Y., Peng, H., Yang, P., Yuan, S., Zhang, X., Wu, Y., Huang, M., & Li, J. (2021). Effect of Intermittent Fasting on Non-Alcoholic Fatty Liver Disease: Systematic Review and Meta-Analysis. Frontiers in nutrition, 8, 709683. https://doi.org/10.3389/fnut.2021.709683
Zubrzycki, A., Cierpka-Kmiec, K., Kmiec, Z., & Wronska, A. (2018). The role of low-calorie diets and intermittent fasting in the treatment of obesity and type-2 diabetes. Journal of physiology and pharmacology: an official journal of the Polish Physiological Society, 69(5). https://doi.org/10.26402/jpp.2018.5.02
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Copyright (c) 2022 Fernanda Oliveira Pignaton; Vivian Michele Lourenço; Karla Lírio Soares; Arthur Merigueti de Souza Costa; Ewelyne Miranda Lima ; Marcio Fronza; Rodrigo Scherer
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