Genotoxic Potential of the Metholic Extract of Psidium guineense Sw (Araçá verdadeiro) in Oral Mucosa Cells

Authors

  • Rebeca Cícera Mendes de Oliveira Silva Universidade Federal de Campina Grande
  • Saraghina Maria Donato da Cunha Universidade Federal da Paraíba
  • Cássio Ilan Soares Medeiros Universidade Federal da Paraíba
  • Raline Mendonça dos Anjos Universidade Federal de Campina Grande
  • Heloisa Mara Batista Fernandes de Oliveira Hospital Universitário Ana Bezerra
  • Aleson Pereira de Sousa Universidade Federal da Paraíba
  • Yanna Carolina Ferreira Teles Universidade Federal da Paraíba
  • Abrahão Alves de Oliveira Filho Universidade Federal de Campina Grande

DOI:

https://doi.org/10.33448/rsd-v9i7.3814

Keywords:

Phytotherapy; In vitro; Psidium; Genotoxicity; Plant.

Abstract

The present research seeks to perform the analysis of the genotoxic potential of the methanolic extract of the plant Psidium (P.) guineense Sw, popularly known as ‘Araçá Verdadeiro’, against cells in the oral cavity. The donor's oral mucosa cells were exposed ex vivo to the methanolic extract of P. guineense in concentrations (50, 100, 500 and 1000 µg/mL) for 30 minutes, the cell pellet smear was made and subsequent fixation on slides stained with 2% Giemsa. The analysis was done using an optical microscope, the whole experiment carried out in triplicate. The data reveal that even at high concentrations (500 and 1000 µg/mL) of the methanolic extract of P. guineense Sw. Cell toxicity is considered low with a percentage of normal cells (94.5% to 94%) compatible with the negative control. Therefore, the methanolic extract, belonging to the species P. guineense Sw. has the bioactive potential that can be explored through the possible application of this compound as a phytotherapic, however more studies must be performed to better understand the toxicity profile of this product.

References

Alborghetti, G, Casanova, M, & Oliveira, T. (2015). Evaluación de la genotoxicidad inducida por la administración repetida de anestésicos locales: Un estudio experimental en ratones. Rev Bras Anestesiol, 65(1), 21-26.

Caldeira, SD, Hiane, PA, Ramos, MIL, & Ramos Filho, MM. (2004). Caracterização físico-química do araçá (Psidium guineense Sw.) e do tarumã (Vitex cymosa Bert.) do estado de Mato Grosso do Sul. Boletim do Centro de Pesquisa de Processamento de Alimentos, 22(1).

Carvalho, JD. (2006). Atividade antiulcerogênica e anticâncer de produtos naturais e de síntese. Rev Multic, 7, 1-18.

Cerqueira, EMM, Gomes-Filho, IS, Trindade, S, Lopes, MA, Passos, JS & Machado-Santelli, GM (2004). Danos genéticos em células esfoliadas da mucosa oral de indivíduos expostos a raios-X durante radiografias odontológicas panorâmicas. Pesquisa de mutação / toxicologia genética e mutagênese ambiental , 562(1-2), 111-117.

Fernandes, TG, Mesquita, ARC, Randau, KP, Franchitti, AA, & Ximenes, EA. (2012). In vitro synergistic effect of Psidium guineense (Swartz) in combination with antimicrobial agents against methicillin-resistant Staphylococcus aureus strains. The Scientific World Journal, 2012.

Gabriel, HE, Crott, JW, Ghandour, H., Dallal, GE, Choi, SW, Keyes, MK, ... & Mager, D. (2006). O tabagismo crônico está associado à diminuição do estado folato, alteração na distribuição da forma folato e aumento do dano genético na mucosa bucal de adultos saudáveis. O jornal americano de nutrição clínica , 83(4), 835-841.

Neira González, AM, Ramírez González, MB, & Sánchez Pinto, NL. (2005). Estudio fitoquímico y actividad antibacterial de Psidium guineense Sw (choba) frente a Streptococcus mutans, agente causal de caries dentales. Revista cubana de plantas medicinales, 10(3-4), 0-0.

Kassie, F, Darroudi, F, Kundi, M, Schulte-Hermann, R, & Knasmüller, S. (2001). Khat (Catha edulis) consumption causes genotoxic effects in humans. International journal of cancer, 92(3), 329-332.

Luz, EB, Silva, RPD, Reis, TA, Leite, ADS, & Lima, MMDO. (2012, August). Avaliação da resposta do chá branco (Camellia sinensis) industrializado frente aos testes Allium cepa e micronúcleo ex-vivo em mucosa oral. In VII CONNEPI-Congresso Norte Nordeste de Pesquisa e Inovação.

Nasciutti,PR. (2012). Desenvolvimento de novos fármacos. Disponível em: <http://ppgca.evz.ufg.br/up/67/o/1%C2%B0_Semin%C3%A1rio_PRISCILLA_NASCIUTTI.pdf?1350665635>. Acesso em: 17 mar. 2020.

Paulo, PTC, Diniz, MDF, Medeiros, IAD, Morais, LC, Andrade, FBD, & Santos, HB. (2009). Ensaios clínicos toxicológicos, fase I, de um fitoterápico composto (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill). Revista Brasileira de Farmacognosia, 19(1A), 68-76.

Pereira, A.S. et al. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Acesso em: 2 maio 2020. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Silva, ADN, Bomfim, HF, Magalhães, AO, Rocha, MLD, & Lucchese, AM. (2018). Composição química e atividade antinociceptiva em modelo animal do óleo essencial de Myrcia rostrata DC.(MYRTACEAE). Química Nova, 41(9), 982-988.

Sobral, M, Proença, C, Souza, M, Mazine, F, & Lucas, E. (2015). Myrtaceae in lista de espécies da flora do Brasil. Jardim Botânico do Rio de Janeiro.

Souza, LJD. (2015). A investigação do conhecimento e uso de plantas medicinais na região do Distrito Prata, município de Monte Alegre de Goiás-GO.

Thomas, P, Harvey, S, Gruner, T, & Fenech, M. (2008). The buccal cytome and micronucleus frequency is substantially altered in Down's syndrome and normal ageing compared to young healthy controls. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 638(1-2), 37-47.

Tolbert, PE., Shy, CM., & Allen, JW. (1991). Micronuclei and other nuclear anomalies in buccal smears: a field test in snuff users. American Journal of Epidemiology, 134(8), 840-850.

Valente, RO., Sampaio, FC., Souza, IA., & Higino, JS. (2009). Estudo toxicológico pré-clínico (agudo) do extrato do Syzygium aromaticum (L) em roedores. Revista Brasileira de Farmacognosia, 19(2B), 557-560.

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Published

05/05/2020

How to Cite

SILVA, R. C. M. de O.; CUNHA, S. M. D. da; MEDEIROS, C. I. S.; ANJOS, R. M. dos; OLIVEIRA, H. M. B. F. de; SOUSA, A. P. de; TELES, Y. C. F.; OLIVEIRA FILHO, A. A. de. Genotoxic Potential of the Metholic Extract of Psidium guineense Sw (Araçá verdadeiro) in Oral Mucosa Cells. Research, Society and Development, [S. l.], v. 9, n. 7, p. e214973814, 2020. DOI: 10.33448/rsd-v9i7.3814. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/3814. Acesso em: 18 nov. 2024.

Issue

Vol. 9 No. 7

Section

Health Sciences

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