Detection of Leishmania spp. in canine serum fixed in card
DOI:
https://doi.org/10.33448/rsd-v11i16.38434Keywords:
FTA ELUTE CARDS®; Molecular diagnosis; Leishmaniasis; Leishmania.Abstract
Visceral leishmaniasis is a serious zoonosis, and its molecular diagnosis is an important strategy. The detection of Leishmania spp. DNA from biological samples fixed in cards could solve problems related to the collect and transport of biological samples. The purpose of this study was to detect Leishmania spp. by PCR, using canine serum fixed in cards (CSFC). For evaluation, the PCR with DNA extracted from CSFC (PCR/card) was compared to the PCR with DNA from the same serum extracted using the Qiagen kit (PCR/Qiagen) and the immunochromatography test (Rapid test - RT). The results showed that out of the 112 analyzed samples, 12 (10.71%) were positive in the PCR/Qiagen, and of those, only two failed to amplify with DNA extracted from the CSFC. The RT was positive in 11 (9.8%) samples; however, only 3 (2.75%) samples of the total agreed with the detection of Leishmania spp. through PCR. Considering the PCR/Qiagen test as the reference standard for DNA detection, the agreement with the PCR/card was nearly perfect (K = 0.8). The concentration and purity of DNA in the two extractions were not significantly different. The use of PCR with DNA from CSFC showed that it can be an interesting alternative for the diagnosis of leishmaniasis.
References
Ali N., Bello G.L., Rossetti M.L.R., Krieger M.A. & Costa A.D.T. (2020). Demonstration of a fast and easy sample-toanswer protocol for tuberculosis screening in point-of-care settings: A proof of concept study. Plos One, 15(12): e0242408.
Almeida A.B.P.F., Sousa V.R.F., Gasparetto N.D., Silva G.F.R., Figueiredo F.B., Dutra V., Nakazato L. & Madeira M.F. (2013). Canine visceral leishmaniasis: diagnostic approaches based on polymerase chain reaction employing different biological samples. Diagnostic Microbiology and Infectious Disease,76(3):321-324.
Antunes T.R., Godoy K.C.S., Oliveira G.G., Silveira A.W., Ramos C.A.N.R. & Souza A.I. (2018). Técnicas de citologia aspirativa, biópsia e citobloco de medula óssea para identificação e determinação de intensidade parasitária na leishmaniose visceral canina. Arquivo Brasileiro de Medicina Veterinária e Zootecnia,70(5);1362-1368.
Assis T.S.M., Braga A.S.C., Pedras M.J., Barral A.M.P., Siqueira I.C., Costa C.H.N., Costa D.L., Holanda T.A., Soares V.Y.R, Biá M., Caldas A.J.M., Romero G.A.S. & Rabello A. (2018). Validação do teste imunocromatográfico rápido IT-LEISH® para o diagnóstico da leishmaniose visceral humana. Revista Epidemiologia e Serviços de Saúde, 17(2):107-111.
Bankamp B., Sein C., Pukuta Simbu E., Anderson R., Abernathy E., Chen M-H., Muyembe Tamfum J-J., Wannemuehler K.A., WakuKouomou D., Lopareva E.N., Icenogle J.P., Rota P.A., Goodson JL. 2019. Use of FTA cards to transport throat swabs and oral fluid samples for molecular detection and genotyping of measles and rubella viruses. Journal of Clinical Microbiology, 57:e00048-19.
Brasil (2014) Ministério da Saúde. Manual de vigilância e controle da leishmaniose visceral. http://www.saude.ba.gov.br/wp-content/uploads/2019/05/2014-Manual-de-vigililanciancia-e-controle-da-leishmaniose-visceral.pdf.
Carvalho F.L.N., Ribaldo E.O., Bello G.L., Ramos R.R., Barcellos R.B., Gehlen M., Halon M.L., Ramão P.R.R., Dellegrave E. & Rossetti M.L.R. (2018). Diagnosis of canine visceral leishmaniasis: comparative performance of serological and molecular tests in symptomatic and asymptomatic dogs. Epidemiology and Infection, 146(5) 571-576.
Cox A.P., Tosas O., Tilley A., Picozzi K., Coleman P. Hide G. & Welburn S.C. (2010). Constraints to estimating the prevalence of trypanosome infections in East African zebu cattle. Parasites & Vectors, 3(1):1-8.
De Brito R.C.F., Aguiar-Soares R.D.O., Cardoso J.M.O., Coura-Vital W., Roatt B.M. & Reis A.B. (2020). Recent advances and new strategies in Leishmaniasis diagnosis. Applied Microbiology and Biotechnology, 104(19):8105-8116.
Farahmand M. & Nahrevanian H. (2016). Application of recombinant proteins for serodiagnosis of visceral leishmaniasis in humans and dogs. Iranian Biomedical Journal, 20(3):128.
González-Marcano E., Kato H., Concepción J.L., Márquez M.E. & Mondolfi A.P. (2016). Polymerase chain reaction diagnosis of leishmaniasis: A species-specific approach. Clinical Applications of PCR, 1392:113-114.
Lalani T., Tisdale M.D., Liu J., Mitra I., Philip C., Odundo E., Reyes F., Frases J.A, Huthey E., Connor P., Swierczewski B.E., Houpt E., Tribble D.R & Riddle M.S. (2018). Comparison of stool collection and storage on Whatman FTA Elute cards versus frozen stool for enteropathogen detection using the TaqMan Array Card PCR assay. Plos. One, 3(8):e0202178.
Lipic S., Giordullo L.M. & Fredericks J.D. (2018). A novel FTA™ elute card collection method that improves direct DNA amplification from bloodstained concrete. Science & Justice, 58(5):303-307.
Mabayi A., Paredes M. & Ospina J. (2018). A comparative assessment of epidemiologically different cutaneous leishmaniasis outbreaks in Madrid, Spain and Tolima, Colombia: an estimation of the reproduction number via a mathematical model. Tropical medicine and infectious disease, 3(2):43.
Maia-Elkhouri A.N.S., Romero G.A.S., Valadas S.Y.O.B., Sousa-Gomes M.L., Lindoso J.A.L., Cupolillo E., Ruiz-Postigo J.A. Argaw D. & Sanchez-Vazquez M.J. (2019). Premature deaths by visceral leishmaniasis in Brazil investigated through a cohort study: A challenging opportunity?. PLOS Neglected Tropical Diseases, 13(12):e0007841.
Millán J., Ferrogli E. & Solano-Gallego L. (2014). Role of wildlife in the epidemiology of Leishmania infantum infection in Europe. Parasitology Research, 113(6):2005-2014.
Mota, C. A., Venazzi, E., Zanzarini, P. D., Aristides, S., Lonardoni, M., & Silveira, T. (2020). Filter paper performance in PCR for cutaneous leishmaniasis diagnosis. Revista da Sociedade Brasileira de Medicina Tropical, 54, e00472020.
Organização Pan-Americana da Saúde. PAHO (2020). Informe Epidemiológico das Américas. https://iris.paho.org/handle/10665.2/53091
Paiva-Cavalcanti M., Morais R.C.S., Pessoa-e-Silva R.C.S, Trajano-silva L.A.M., Gonçalves-de-Albuquerque S. C., Tavares D.H.C., Brelaz-de-Castro M.C.A., Silva R.F. & Pereira V.R.A. (2015) Leishmaniasis diagnosis: an update on the use of immunological and molecular tools. Cell & bioscience, 5(1):31.
Palido-Landínez M., Laviniki V., Sánchez-Ingunza R., Guard J. & Nascimento V.P. (2012). Uso dos cartões FTA para o transporte de amostras de DNA de Salmonella spp. isoladas de produtos avícolas do Sul do Brasil. Acta Scientiae Veterinariae, 40(4)1-7.
Quinnell R.J. & Courtenay O. (2009). Transmission, reservoir hosts and control of zoonotic visceral leishmaniasis. Parasitology,136(14):1915-1934.
Ribeiro, R. R., Michalick, M., da Silva, M. E., Dos Santos, C., Frézard, F., & da Silva, S. M. (2018). Canine Leishmaniasis: An Overview of the Current Status and Strategies for Control BioMed research international,12.
Riboldi E., Carvalho F., Romão P.R.T, Barcellos R.G, Bello G.L., Ramos R.R., Oliveira R.T., Júnior J.P.A., Rossetti M.L & Dallegrave E. (2018). Molecular method confirms canine Leishmania infection detected by serological methods in non-endemic area of Brazil. The Korean Journal of Parasitology, 56(1):11.
Rolim F., Carvalho F.L.N., Bello G.L., Gehlen M., Halon M.L., Lemos R.R., Barcellos R.B. & Rossetti M.L. (2016). Leishmaniose visceral canina: detecção de DNA em soro por PCR em tempo real. Revista de Iniciação Científica da ULBRA, (14):36-46.
Sagi, O., Berkowitz, A., Codish, S., Novack, V., Rashti, A., Akad, F., & Shemer-Avni, Y. (2017). Sensitive Molecular Diagnostics for Cutaneous Leishmaniasis. Open forum infectious diseases, 4(2): ofx037.
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985). Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230(4732): 1350–1354.
Santos J.M.L., Dantas-torres F., Mattos M.R.F., Lino F.R.L., Andrade L.S.S., Souza R.C.A., Brito F.L.C., Brito M.E.F., Brandão-filho S.P. & Simões-Mattos L. (2010). Prevalência de anticorpos antileishmania spp em cães de Garanhuns, Agreste de Pernambuco. Revista da Sociedade Brasileira de Medicina Tropical43:41-45.
Secretaria de Estado de Saúde de Santa Catarina. (2018) Guia de Orientação para a vigilância da leishmaniose visceral canina (LVC) disponível em: http://www.dive.sc.gov.br/conteudos/zoonoses/publicacoes/Guia_Basico_de_Orientacao_LVC_2018.
Silva J.D., Melo D.H.M., Costa J.A.G., Costa D.F., Silva R.B.S., Melo M.A. & Alves C.J. (2017). Leishmaniose visceral em cães de assentamentos rurais. Pesquisa Veterinária Brasileira, 37(11):1292-1298.
Silva, R. D. S., & Paes, A. T. (2012). Por dentro da estatística: Teste de concordância Kappa. Educação Continuada em Saúde Einstein, 10(4):165-166.
Souza Y.C.P., Carvalho A.F.S., Carvalho L.A.R. & Mansur V.F.R. (2013). Teste diagnóstico para leishmaniose visceral – atualidade e perspectivas. 11(21)1-16. Retrieved from http://faef.revista.inf.br/imagens_arquivos/arquivos_destaque/jMGetvi4ZMFD9rK_2013-8-14-17-14-35.pdf
Teixeira M.C., Stobbe N.S., Schmidt V., Lima V.M.F., Tartoratti A.L, Ramos R.R & Araujo F.A.P. (2016). Seroepidemiology of Leishmania infantum in dogs in the city of Porto Alegre, Rio Grande do Sul. Semina: Ciências Agrárias, 37(6).
Tlamcani Z. (2016). Visceral leishmaniasis: an update of laboratory diagnosis. Asian Pacific Journal of Tropical Disease, 6(7):505-508.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Ianca Moraes dos Santos Petry ; Fernanda dos Santos Rolim; Tainá dos Santos Soares; Maria Eduarda Rossetti de Carvalho; Josiane Somariva Prophiro; Diego Soares Leote ; Maria Lucia Rossetti
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
