Photon flux density and cytokinin types influence biomass production and volatile organic compound accumulation in Dysphania ambrosioides L under in vitro conditions
DOI:
https://doi.org/10.33448/rsd-v12i2.39841Keywords:
Medicinal plant; Micropropagation; Plant growth regulator; Light.Abstract
This study aimed to evaluate different intensity of irradiance and cytokinins concentration which can be influential in growth and volatile compounds production in vitro for Dysphania ambrosioides L., a plant present in the RENISUS list for medicinal plants with high interest in research and use. The irradiance intensities of 16.5, 36.6, 47.6 and 73.2 µmol m-2s-1 in the growth room were evaluated for nodal segments development. Three kinds of cytokinin (BAP, TDZ and Kinetin) in four different concentrations (0.0, 0.5, 1.0 and 2.0 mg L-1) were studied. Nodal segments were kept in growth room under 47.6 µmol m-2s-1 of irradiance and without cytokinin was found suitable for nodal segment growth in vitro. The number of volatile compounds of this species was not affected by irradiance intensity or cytokinin, however, the concentrations were affected.
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Copyright (c) 2023 Alexandre Alves de Carvalho; Rafael Marlon Alves de Assis; João Pedro Miranda Rocha ; Jeremias José Ferreira Leite; Flávia Dionisio Pereira; Jandeilson Pereira dos Santos; Suzan Kelly Vilela Bertolucci; José Eduardo Brasil Pereira Pinto
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