Influence of immersion solution and dentin interaction on the solubility of ready-to-use or powder-liquid bioceramic endodontic sealers

Authors

DOI:

https://doi.org/10.33448/rsd-v12i3.40216

Keywords:

Calcarea silicata; Endodontics; Solubility.

Abstract

The aim of this study was to evaluate the effect of immersion solution and interaction with dentin on the solubility of ready-to-use bioceramic NeoSEALER® Flo (NSF) or powder-liquid BioRoot RCS (BR). Molds with an internal diameter of 5 mm and a length of 1.5 mm were made of polyethylene or bovine dentin. After filling with NSF or BR, they were kept in an oven for 24 hours. After determining the initial mass on a precision scale, the specimens were immersed in 7.5 mL of distilled water (DW) or phosphate buffered saline solution (PBS) (n=8) for 7 or 14 days. The final mass was obtained after weighing until mass stabilization occurred. Solubility was evaluated according to the difference between the initial and final mass in percentage. Two-way ANOVA, Bonferroni and unpaired t-tests were used (α=0.05). After 7 days, both materials showed lower solubility in PBS than DW (p<0.05). Filling mold did not influence solubility (p>0.05), except for polyethylene/NSF in DW after 7 days (p<0.05). NSF showed lower solubility than BR in DW (dentin) or PBS (polyethylene) after 14 days (p<0.05). It is concluded that PBS provides lower solubility than DW for NSF and BR after 7 days. Polyethylene mold influences NSF solubility after 7 days. The immersion solution does not influence the mass change after 14 days. New methodological approaches may represent important alternatives for evaluating the solubility of bioceramic sealers.

References

Alves Silva, E. C., Tanomaru-Filho, M., da Silva, G. F., Delfino, M. M., Cerri, P. S., & Guerreiro-Tanomaru, J. M. (2020). Biocompatibility and bioactive potential of new calcium silicate-based endodontic sealers: Bio-C Sealer and Sealer Plus BC. J Endod, 46(10), 1470-1477.

Amaral, C. S. dos S.., Tomaz, A. F. G.., Campos, I. J. de O.., Medeiros, E. R. de., Paula, J. V. F. de., Costa, E. H. P. da., Silva, A. F. da., Figueiredo, F. M. P.., & Teixeira, A. P. A. (2020). Current concepts related to the use of bioceramic materials in Dentistry. Research, Society and Development, 9(12), e34191211277.

American National Standards Institute/American Dental Association (ANSI/ADA). Specification no. 57 https://www.ada.org/resources/practice/dental-standards/standards-committee-on-dental-products/products-standards-technical-specifications-and-technical-reports

Bardají, D. K. R., Moretto, J. A. S., Furlan, J. P. R., & Stehling, E. G. (2020). A mini-review: current advances in polyethylene biodegradation. World J Microbiol Biotechnol, 36(2), 32.

Bastos, L. A. P., Vitti, R. P., & Casonato Junior, H. (2021). Sorption and solubility of bioceramic endodontic sealers and sealer based on epoxy resin. Research, Society and Development, 10(7), e31210716676.

Bortoluzzi, E. A., Cassel de Araújo, T., Carolina Corrêa Néis, A., Cássia Dos Santos, M., da Fonseca Roberti Garcia, L., Dulcinéia Mendes Souza, B., & da Silveira Teixeira, C. (2019). Effect of different water-to-powder ratios on the dimensional stability and compressive strength of mineral aggregate-based cements. Eur Oral Res, 53(2), 94-98.

Cavenago, B. C., Pereira, T. C., Duarte, M. A., Ordinola-Zapata, R., Marciano, M. A., Bramante, C. M., & Bernardineli, N. (2014). Influence of powder-to-water ratio on radiopacity, setting time, pH, calcium ion release and a micro-CT volumetric solubility of white mineral trioxide aggregate. Int Endod J, 47(2), 120-126.

Debelian, G.; Trope, M. (2016). The use of premixed bioceramic materials in endodontics. G. Ital. Di Endod, 30, 70-80.

Duque, J. A., Fernandes, S. L., Bubola, J. P., Duarte, M. A. H., Camilleri, J., & Marciano, M. A. (2018). The effect of mixing method on tricalcium silicate-based cement. Int Endod J, 51(1), 69-78.

Elyassi, Y., Moinzadeh, A. T., & Kleverlaan, C. J. (2019). Characterization of leachates from 6 root canal sealers. J Endod, 45(5), 623-627.

Gandolfi, M. G., Siboni, F., Botero, T., Bossù, M., Riccitiello, F., & Prati, C. (2015). Calcium silicate and calcium hydroxide materials for pulp capping: biointeractivity, porosity, solubility and bioactivity of current formulations. J Appl Biomater Funct Mater, 13(1), 43-60.

Gaudin, A., Tolar, M., & Peters, O. A. (2020). Cytokine production and cytotoxicity of calcium silicate-based sealers in 2- and 3-dimensional cell culture models. J Endod, 46(6), 818-826.

International Organization for Standardization (ISO 6876:2012). Dental root canal sealing materials https://www.iso.org/standard/45117.html

Jung, S., Sielker, S., Hanisch, M. R., Libricht, V., Schäfer, E., & Dammaschke, T. (2018). Cytotoxic effects of four different root canal sealers on human osteoblasts. PloS one, 13(3), e0194467.

Katakidis, A., Sidiropoulos, K., Koulaouzidou, E., Gogos, C., & Economides, N. (2020). Flow characteristics and alkalinity of novel bioceramic root canal sealers. Restor Dent Endod, 45(4), e42.

Khanam PN, AlMaadeed MAA. (2015). Processing and characterization of polyethylene-based composites. Adv Manuf Polym Compos Sci,1:63-79.

Kharouf, N., Arntz, Y., Eid, A., Zghal, J., Sauro, S., Haikel, Y., & Mancino, D. (2020). Physicochemical and antibacterial properties of novel, premixed calcium silicate-based sealer compared to powder-liquid bioceramic sealer. J Clin Med, 9(10), 3096.

Kikly, A., Jaâfoura, S., Kammoun, D., & Sahtout, S. (2020). Sealing ability of endodontic cements: An in vitro study. Int J Dent, 5862598.

Kim, Y., Kim, B. S., Kim, Y. M., Lee, D., & Kim, S. Y. (2019). The penetration ability of calcium silicate root canal sealers into dentinal tubules compared to conventional resin-based sealer: a confocal laser scanning microscopy study. Materials (Basel), 12(3), 531.

Loison-Robert, L. S., Tassin, M., Bonte, E., Berbar, T., Isaac, J., Berdal, A., Simon, S., & Fournier, B. P. J. (2018). In vitro effects of two silicate-based materials, Biodentine and BioRoot RCS, on dental pulp stem cells in models of reactionary and reparative dentinogenesis. PloS one, 13(1), e0190014.

Long, J., Kreft, J. U., & Camilleri, J. (2020). Antimicrobial and ultrastructural properties of root canal filling materials exposed to bacterial challenge. J Dent, 93, 103283.

López-García, S., Myong-Hyun, B., Lozano, A., García-Bernal, D., Forner, L., Llena, C., Guerrero-Gironés, J., Murcia, L., & Rodríguez-Lozano, F. J. (2020). Cytocompatibility, bioactivity potential, and ion release of three premixed calcium silicate-based sealers. Clin oral Investig, 24(5), 1749-1759.

Pramila R, Ramesh KV. (2011). Biodegradation of low-density polyethylene (LDPE) by fungi isolated from marine water—a SEM analysis. Afr J Microbiol Res,5:5013–5018.

Prüllage, R. K., Urban, K., Schäfer, E., & Dammaschke, T. (2016). Material properties of a tricalcium silicate-containing, a mineral trioxide aggregate-containing, and an epoxy resin-based root canal sealer. J Endod, 42(12), 1784-1788.

Reyes-Carmona, J. F., Felippe, M. S., & Felippe, W. T. (2010). A phosphate-buffered saline intracanal dressing improves the biomineralization ability of mineral trioxide aggregate apical plugs. J Endod, 36(10), 1648-1652.

Reyhani, M. F., Ghasemi, N., Zand, V., & Mosavizadeh, S. (2017). Effects of different powder to liquid ratios on the push out bond strength of CEM cement on simulated perforations in the furcal area. J Clin Exp Dent, 9(6), e785-e788.

Sfeir, G., Zogheib, C., Patel, S., Giraud, T., Nagendrababu, V., & Bukiet, F. (2021). Calcium silicate-based root canal sealers: a narrative review and clinical perspectives. Materials (Basel), 14(14), 3965.

Siboni, F., Taddei, P., Zamparini, F., Prati, C., & Gandolfi, M. G. (2017). Properties of bioRoot RCS, a tricalcium silicate endodontic sealer modified with povidone and polycarboxylate. Int Endod J, 50 Suppl 2, e120-e136.

Silva, D. F. da., Silva, L. L. C. da., Wanderley, C. T. B., & Vasconcelos, R. A. de. (2020). Bioceramic sealers in endodontics: integrative review. Research, Society and Development, 9(8), e882986439.

Silva, E. J. N. L., Ferreira, C. M., Pinto, K. P., Barbosa, A. F. A., Colaço, M. V., & Sassone, L. M. (2021). Influence of variations in the environmental pH on the solubility and water sorption of a calcium silicate-based root canal sealer. Int Endod J, 54(8), 1394-1402.

Tavares, K. I. M. C., Pinto, J. C., Santos-Junior, A. O., Torres, F. F. E., Guerreiro-Tanomaru, J. M., & Tanomaru-Filho, M. (2021). Micro-CT evaluation of filling of flattened root canals using a new premixed ready-to-use calcium silicate sealer by single-cone technique. Microsc Res Tech, 84(5), 976-981.

Torres, F. F. E., Bosso-Martelo, R., Espir, C. G., Cirelli, J. A., Guerreiro-Tanomaru, J. M., & Tanomaru-Filho, M. (2017). Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests. J Appl Oral Sci, 25(4), 374-380.

Torres, F. F. E., Guerreiro-Tanomaru, J. M., Pinto, J. C., & Tanomaru-Filho, M. (2021). Effect of different dimensions of test samples on the volumetric change assessment of endodontic materials. Braz Dent J, 32(1), 42-47.

Torres, F. F. E., Zordan-Bronzel, C. L., Guerreiro-Tanomaru, J. M., Chávez-Andrade, G. M., Pinto, J. C., & Tanomaru-Filho, M. (2020). Effect of immersion in distilled water or phosphate-buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate-based root canal sealers. Int Endod J, 53(3), 385-391.

Urban, K., Neuhaus, J., Donnermeyer, D., Schäfer, E., & Dammaschke, T. (2018). Solubility and pH value of 3 different root canal sealers: A long-term investigation. J Endod, 44(11), 1736–1740.

Vo, K., Daniel, J., Ahn, C., Primus, C., & Komabayashi, T. (2022). Coronal and apical leakage among five endodontic sealers. J Oral Sci, 64(1), 95-98.

Zamparini, F., Prati, C., Taddei, P., Spinelli, A., Di Foggia, M., & Gandolfi, M. G. (2022). Chemical-physical properties and bioactivity of new premixed calcium silicate-bioceramic root canal sealers. Int J Mol Sci, 23(22), 13914.

Zhao, P., Lu, C., Gao, X. P., Yao, D. H., Cao, C. L., & Luo, Y. J. (2018). Regulating the microstructure of intumescent flame-retardant linear low-density polyethylene/nylon six blends for simultaneously improving the flame retardancy, mechanical properties, and water resistance. ACS omega, 3(6), 6962-6970.

Zordan-Bronzel, C. L., Esteves Torres, F. F., Tanomaru-Filho, M., Chávez-Andrade, G. M., Bosso-Martelo, R., & Guerreiro-Tanomaru, J. M. (2019). Evaluation of physicochemical properties of a new calcium silicate-based sealer, Bio-C Sealer. J Endod, 45(10), 1248-1252.

Published

21/02/2023

How to Cite

GONÇALVES, A. A.; SANTOS-JUNIOR, A. O.; TAVARES, K. I. M. C. .; PINTO, J. C. .; GUERREIRO-TANOMARU, J. M. .; TANOMARU-FILHO, M. Influence of immersion solution and dentin interaction on the solubility of ready-to-use or powder-liquid bioceramic endodontic sealers. Research, Society and Development, [S. l.], v. 12, n. 3, p. e5512340216, 2023. DOI: 10.33448/rsd-v12i3.40216. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/40216. Acesso em: 23 apr. 2024.

Issue

Section

Health Sciences