Correlation between urocultures and urine summary for the diagnosis of urinary tract infection in patients admitted to a university hospital in the state of Paraíba

Authors

DOI:

https://doi.org/10.33448/rsd-v12i4.41121

Keywords:

Urinary sediment analysis; Urinary tract infections; Nitrite; Pyuria.

Abstract

Urinary tract infection (UTI) is one of the most frequent infections that affect patients in hospitals and can be classified as cystitis or pyelonephritis, depending on the site of infection. The diagnosis of UTI is basically made by performing the urine summary (EAS) and urine culture. Thus, the objective of this study was to conduct a retrospective study from January/2021 to December/2021 based on the database of the clinical analysis laboratory of the University Hospital Lauro Wanderley (HULW) in the state of Paraiba in relation to the alterations presented in the EAS exam, mainly with the markers nitrite and leukocyte esterase from positive urine cultures. The epidemiological data of these infections was also observed, as well as their bacterial incidence. A total of 1817 urine cultures were obtained, of which 837 had a request for EAS concomitantly, and of these 129 urine culture samples were positive, with the prevalent microorganisms being Escherichia coli (36.5%), Klebsiella pneumoniae (20.6 %) and Pseudomonas aeruginosa (8.7%). Nitrite was observed in 16% of the samples and leukocyte esterase in 58%. We conclude that regarding the results of the EAS, leukocyturia proved to be a more sensitive marker to aid in the diagnosis of UTI compared to nitrite, corroborating the specialized scientific literature, with a predominance of UTI in female patients.

Author Biographies

Viviane Araújo Silva, Hospital Universitário Lauro Wanderley

Bióloga do HULW-UFPB. Pesquisadora do CNPQ de projeto de iniciação científica do programa PIC-EBSERH.

Brenda Kettinly Ferreira Souza, Universidade Federal da Paraíba

Acadêmica do curso de farmácia, pesquisadora de iniciação científica CNPQ do projetos PIC-EBSERH-HULW

Larissa Rodrigues dos Santos Silva, Hospital Universitário Lauro Wanderley

Biomédica do laboratório de microbiologia do HULW

Ana Paloma Tavares de Araújo, Hospital Universitário Lauro Wanderley

Bióloga do Hospital Universitário Lauro Wanderley

Francisco Cunha Nunes, Hospital Universitário Lauro Wanderley

Biomédico e técnico de laboratório do Hospital Universitário Lauro Wanderley

Humberto de Carvalho Aragão Neto, Universidade Federal da Paraíba

Farmacêutico e técnico do laboratorio de análises clínicas do HULW

Nathalia de Alencar Cunha Tavares, Hospital Universitário Lauro Wanderley

Biomédica do Hospital Universitário Lauro Wanderley

References

Alves, A. R. D. M. S. (2018). Estratégias profiláticas da infeção urinária recorrente não complicada na mulher adulta saudável (tese de doutorado). Universidade do Porto, Portugal.

Blatt, J. M., & Miranda, M. C. (2005). Perfil dos microrganismos causadores de infecções do trato urinário em pacientes internados. Revista panamericana de Infectologia, 9(4), 10-14.

Bortolotto, L. A., Indras, D. M., Silva, C. M., & Peder, L. D. (2016). Presença de analitos químicos e microscópicos na urina e sua relação com infecção urinária. Revista Saúde, 42(2), 89-96.

Castro, B. G. (2019). Prevalência de bactérias Gram-positivas em infecção do trato urinário. Revista Brasileira de Análises clínicas, 51(4), 322-337.

Chaves, N. M. O., & Moraes, C. L. K. (2015). Control of infection in urinary catheterization in an intensive care unit. Revista de Enfermagem do Centro Oeste Mineiro, 5(2), 1650-1657.

Cristea, O. M., Avrămescu, C. S., Bălășoiu, M., Popescu, F. D., Popescu, F., & Amzoiu, M. O. (2017). Urinary tract infection with Klebsiella pneumoniae in Patients with Chronic Kidney Disease. Curr Health Sci J, 43(2), 137-148.

Furlan, A. P. F., Pereira, R. R. S., Xavier, R. A., & Lima, T. S. (2021). Prevalência e perfil de resistência bacteriana nas infecções do trato urinário em hospitais da região norte e nordeste do Brasil: uma revisão. Brazilian Journal of Health Review, 4(2), 9244-9256.

Henry, J. B., Lauzon, R. B., & Schumann, G. B. (1996). Basic Examination of urine. In: Henry JB, editor. Clinical diagnosis and management by laboratory methods. Philadelphia, WB Saunders; p. 411-456.

Klein, R. D., & Hultgren, S. J. (2020). Urinary tract infections: microbial pathogenesis, host-pathogen interactions, and new treatment strategies. Nature Reviews Microbiology, 18(4), 211-226.

Koche, J. C. (2011). Fundamentos de metodologia científica. Petrópolis: Vozes. http://www.brunovivas.com/wp content/uploads/sites/10/2018/07/K%C3%B6che-Jos%C3%A9-

Carlos0D0AFundamentos-de-metodologia-cient%C3%ADfica-_-teoria da0D0Aci%C3%AAncia-e-inicia%C3%A7%C3%A3o-%C3%A0-

pesquisa.pdfhttps://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1

Masson, L.C., Ferreira, A.L., Oliveira, A.F., Barcellos, R. B., Carvalho, A.L., Silva, G. A., ... & Oliveira, R. A. (2020). Diagnóstico laboratorial das infecções urinárias: relação entre a urocultura e o EAS. Revista Brasileira de Análises Clínicas, 52(1), 77-81.

Miranda, A. L., Oliveira, A. L. L., Nacer, D. T., & Aguiar, C. A. M. (2016). Results after implementation of a protocol on the incidence of urinary tract infection in an intensive care unit. Revista Latino-Americana de Enfermagem, 24. doi:10.1590/1518-8345.0866.2804.

Ngong, I. J., Etongwe, E., Atashili, J., Akoachere, J. F. T. K., & Ndumbe, P. M. (2021). Prevalence, antimicrobial susceptibility pattern and associated risk factors for urinary tract infections in pregnant women attending ANC in some integrated health centers in the Buea Health District. BMC Pregnancy and Childbirth, 21(1), 673.

Nys, S., van Merode, T., Bartelds, A. I., & Stobberingh, E. E. (2006). Urinary tract infections in general practice patients: diagnostic tests versus bacteriological culture. Journal of Antimicrobial Chemotherapy, 57(5), 955-958.

Oliveira, M. S., Cardoso, R. S., Santos, J. S., Vasconcelos, J. R., & Souza, M. C. (2021). Principais bactérias encontradas em uroculturas de pacientes com Infecções do Trato Urinário (ITU) e seu perfil de resistência frente aos antimicrobianos. Research, Society and Development, 10(7), e5310716161-e 5310716161.

Ringsrud, K. M., & Linné, J. J. (1995). Chemical examination of urine. In: Ringsrud, K. M. & Linné, J. J. (1995). Urinalysis and body fluids: a color text and atlas. St. Louis (USA): Mosby, p.43-80.

Roriz-Filho, J. S., Oliveira, L. A., Amaral, C. F. S., & Guimarães, P. M. R. (2010). Infecção do trato urinário. Medicina (Ribeirão Preto), 43(2), 118-125.

Guido, S., Eberhardt, K., Klaus, G., Martha, M. M., & Eva, H. P. (2010). The Diagnosis of Urinary Tract Infection. Dtsch Arztebl Int, 107(21), 361-367.

Published

11/04/2023

How to Cite

SILVA, V. A.; SOUZA, B. K. F. .; SILVA, L. R. dos S. .; ARAÚJO, A. P. T. de .; NUNES, F. C. .; ARAGÃO NETO, H. de C. .; TAVARES, N. de A. C. . Correlation between urocultures and urine summary for the diagnosis of urinary tract infection in patients admitted to a university hospital in the state of Paraíba. Research, Society and Development, [S. l.], v. 12, n. 4, p. e19612441121, 2023. DOI: 10.33448/rsd-v12i4.41121. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/41121. Acesso em: 18 nov. 2024.

Issue

Section

Health Sciences