Diluted and cooled bovine semen with different concentrations of a natural compound
DOI:
https://doi.org/10.33448/rsd-v12i5.41398Keywords:
Sperm kinetics; Seminal viability; Antioxidant; Cooling.Abstract
The aim of this study was to analyze the action of a natural extract (NP) added to diluted and refrigerated semen for different periods of time. To evaluate the antioxidant property of the extract, the chosen method was the DPPH (2,2-diphenyl-1-picrylhydrazyl). Initially, the radical was prepared, adding 0.1 mm. - 0.03943g of the DPPH reagent dissolved in 10 mL of 80% methanol, which was then partially (2.9mL) allocated in test tubes with the extract (0.1mL). The samples were then kept at rest and protected from light, and after 30 minutes and 24 hours, the initial and final absorbance measurements were performed, respectively, by means of a spectrophotometer at 515 nm. After the extraction, eight semen collections from two Braford bovines were carried out using an electro-ejaculator. The semen samples were diluted and added to the natural extract, refrigerated and analyzed by CASA (IVOS-IIR, Hamilton Thorne), in order to ascertain the toxicity and antioxidant potential on the spermatozoa, evaluating the effects of the compound on the kinetics of sperm movement and on sperm morphology. Most of the evaluated parameters did not show significant changes (p<0.05). Given the data obtained, it was concluded that the natural extract (NP) has no influence on the kinetics of sperm movement and on the sperm morphology of diluted semen, plus NP and cooled.
References
Almeida, S. et al. (2022) Morfologia espermática em touros: quais os principais defeitos e o porquê ocorrem. Revista Brasileira de Reprodução Animal. 102-110.
Alyethodi, R. R. et al. (2021) Role of seminal MDA, ROS, and antioxidants in cryopreservation and their kinetics under the influence of ejaculatory abstinence in bovine semen. Cryobiology, 98, 187-193.
Brand-Williams, W. et al. (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30.
Bozzi, A. D. R. et al. (2023) Adição de sucos de laranja, abacaxi e beterraba em diluidor para criopreservação de sêmen de carneiros. Ciência Animal Brasileira, v. 24.
Carrer, A. R. (2022) Criopreservação de sêmen bovino com dois diluentes comerciais suplementados com astaxantina. 2022. f.60. Dissertação (Mestrado). Universidade Estadual Paulista, Jaboticabal.
CBRA. Manual para Exame Andrológico e Avaliação de Sêmen Animal. (2a ed.), Colégio Brasileiro de Reprodução Animal, 45p, 2013.
Da Silva, A. F. et al. (2018) Efeito da adição de antioxidantes sobre a viabilidade do sêmen bovino. Arq. Ciênc. Vet. Zool. UNIPAR, 21(4), Anais do II Concivet 2018, 145-146.
De La Rosa, L. A. et al. (2011) Fereidon. Compostos fenólicos e atividade antioxidante de grãos e cascas de noz-pecã mexicana (Carya illinoinensis). Journal of Agricultural and Food Chemistry , 59(1), 152-162.
Felippelli, G., et al. (2023) “Viability of Toxoplasma Gondii in Cattle Semen Cryopreserved with Different Concentrations of Cryoprotectant.” Pesquisa Veterinária Brasileira, 43.
Flores-Cordova, M. et al. (2017) Phytochemical composition and antioxidant capacity in Mexican pecan nut. Emirates Journal of Food and Agriculture, 29(5), 346-350.
Hussain, M. et al. (2018) Additives used in semen preservation in animals: A short review. International Journal of Chemical Studies, 6(5), 354-361.
Kowalczyk, A. et al. (2021) Antioxidant effect of Elamipretide on bull's sperm cells during freezing/thawing process. Andrology, 9(4), 1275-1281.
Leite, P. et al. (2011) Bovine Semen Cryopreservation. Journal of Health Sciences, 13(4), 279-286.
Matos, D. L. et al. (2008) Análise computarizada de espermatozoides: revisão de literatura. Revista Brasileira de Reprodução Animal, 32(4), 225-32.
Mizera, A. et al. (2019) Impact of the Spirulina maxima extract addition to semen extender on bovine sperm quality. Italian Journal of Animal Science, 18(1), 601-607.
Murphy, E. M. et al. (2017) A comparison of semen diluents on the in vitro and in vivo fertility of liquid bull semen. Journal of dairy science, 100(2), 1541-1554.
Pereira, A., S., Shitsuka, D., M., Parreira, F., J. & Shitsuka, R. (2018). Metodologia da pesquisa científica. UFSM. https://repositorio.ufsm.br/.
Pinto, S. C. C. et al. (2020) Does supplementation of vitamin C, reduced glutathione or their association in semen extender reduce oxidative stress in bovine frozen semen? Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 72, 9-17.
Raheja, N. et al. (2018) A review on semen extenders and additives used in cattle and buffalo bull semen preservation. Journal of Entomology and Zoology Studies, 6(3), 239-245.
Rao, K. A. et al. (2017) Human sperm DNA damage inhibition and antioxidant activity of T. arjuna bark: an in vitro study. Biotech, 7, 1-9.
Rostami, B. et al. (2020) Effects of supplementation of tris-egg yolk extender with different sugars and antioxidants on freezability of ram semen. Cryobiology, 92, 62-66.
Rua, M. A. S. et al. (2016) Repetibilidade das características seminais, espermáticas e fertilidade de garanhões. Revista Brasileira de Ciências Agrárias, 11(2), 124-131.
Silva, E. C. B. et al. (2012) Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology, 77(8), 1722-1726.
Silva, N. C. (2017) Uso de antioxidantes em diluentes de criopreservação de sêmen bovino. 2017. 95 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Goiás, Goiânia.
Snoeck, P. P. N. et al. (2020) Padronização do Sperm Class Analyzer®(SCA®) para avaliação de espermatozoides equinos criopreservados. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 72, 1163-1171.
Upadhyay, V. R. et al. (2021) Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview. Andrologia, 53(8), 1-16.
Zhao, X. L. et al. (2015) Genetics and Molecular Research, 14(1), 2572-2581.
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Copyright (c) 2023 Daniel Tobias Bueno Cavalheiro; Camila Keterine Gorzelanski Trenkel; Adalgiza Pinto Neto; Matheus Ramos Rosin; Silmara Mazon; Dalila Moter Benvegnú; Jonatas Cattelam
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