Use of Glutathione and Vitamin C in seminal cryopreservation of Nelore bulls
DOI:
https://doi.org/10.33448/rsd-v12i6.42109Keywords:
Antioxidant; Freezing; Semen; Zebu.Abstract
One way to increase the reproductive efficiency and genetic merit of a herd is by biotechnologies such as artificial insemination and in vitro fertilization, in which frozen semen is essential. Thus, the aim of this study was to compare the concentrations of glutathione and vitamin-C as antioxidant agents, with six ejaculates from six different bulls allocated to each group. Each ejaculate was exposed to four treatments (control/ 1.25mM/ 2.5mM/ 5.0mM) for both glutathione and vitamin C. After cryopreservation, tests were performed to assess seminal quality after thawing through hyposmotic tests, sperm longevity and giemsa/trypan blue. No groups of bulls were contrasted, only treatments within each group. For the glutathione group, there was no significant difference (P>0.05) between the results and the concentrations used, whereas in the vitamin-C group, the 5.0 mM concentration showed unfavorable results compared to the others, indicating damage to the acrosomal membrane, being contraindicated.
References
Amann, R. P. & Pickett, B. W. (1987). Cryopreservation principles and a review of stallion sperm cryopreservation. Journal of Equine Veterinary Science. 7 (3), 145-173.
Arruda, R. P.; Silva, D. F.; Affonso, F. J.; Lemes, K. M.; Jaimes, J. D.; Celeghini, E. D. C.; Alonso, M. A., Carvalho, H. F.; Oliveira, L. Z. & Nascimento, J. (2011). Métodos de avaliação da morfologia e função espermática: momento atual e desafios futuros. Revista Brasileira de Reprodução Animal. 35(2), 145-151.
Arruda, R. L. P., Zúccari, C. E. S. N., Fernandes, C. E., Abreu, U.G.P. & Costa e Silva, E. V. (2013). Características do sêmen congelado relacionadas à fertilidade in vitro em bovinos. Revista Brasileira de Reprodução Animal. 37(1), 72-80. http://www.cbra.org.br/pages/publicacoes/rbra/v37n1/p72-80%20(RB283).pdf
Asadpour, R.; Jafari, R. & Tayefi-Nasrabadi, H. (2012). The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa. Iranian Journal of Veterinary Research. 13(3), 246-249. https://globorural.globo.com/Noticias/Criacao/noticia/2017/09/globo-rural-asbia-venda-de-semen-de-boi-cresce-76-no-primeiro-semestre.html
Associação Brasileira de Inseminação Artificial. (2022). Index ASBIA 2022. https://asbia.org.br/wp-content/uploads/Index/Index_ASBIA_2022.pdf
Ball, B. A.; Vo, A. T. & Baumber, J. (2001). Generation of reactive oxygen species by equine spermatozoa. American journal of veterinary research. 62(4), 508-515.
Baruselli, P. S., Souza, A. H., Sá Filho, M. F., Marques, M. O. & Sales, J. N. S. (2018). Genetic market in cattle (Bull, AI, FTAI, MOET and IVP): financial payback based on reproductive efficiency in beef and dairy herds in Brazil. In: Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianopólis, SC, Brazil, Animal Reproduction. 15(3), 247-255.
Baumber, J., Ball, B. A. & Linfor, J. J. (2005). Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. American journal of veterinary research. 66(5), 772-779.
Bilodeau, P. S.; Urbanowski, J. L.; Winistorfer, S. C. & Piper, R. C. (2002). The Vps27p–Hse1p complex binds ubiquitin and mediates endosomal protein sorting. Nature cell biology. 4(7), 534-539.
Beconi, M. T.; Francia, C. R.; Mora, N. G. & Affranchino, M. A. (1993). Effect of natural antioxidants on frozen bovine semen preservation. Theriogenology. 40(4), 841-851.
Brito, L. F.; Barth, A. D.; Bilodeau-Goeseels, S.; Panich, P. L. & Kastelic, J. P. (2003). Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate. Theriogenology. 60(8), 1539-1551.
Cai, J.; Yang, J. & Jones, D.P. 1998. Mitochondrial control of apoptosis: the role of cytochrome c. Biochim Biophys Acta. 1366(1-2), 139-49.
Donnelly, E. T.; McClure, N. & Lewis, S. E. (1999). The effect of ascorbate and α-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa. Mutagenesis. 14(5), 505-512.
Donnelly, E.T.; McClure, N. & Lewis, S. E. (2000). Glutathione and hypotaurine in vitro: effects on human sperm motility, DNA integrity and production of reactive oxygen species. Mutagenesis. 15, 61-68.
Eidan, S. M. (2016). Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender. Animal Reproduction Science. 167, 1–7. 10.1016/j.anireprosci.2016.01.014 0378-4320/© 2016
Forero-Gonzalez, R. A., Celeghini, E. C.C., Raphael, C. F., Andrade, A. F. C., Bressan, F. F. & Arruda RP. 2012. Effects of bovine sperm cryopreservation using different freezing techniques and cryoprotective agents on plasma, acrosomal and mitochondrial membranes. Andrologia, 44, 159-154.
Gadea, J.; Sellés, E.; Marco, M. A.; Coy, P.; Matás, C.; Romar, R. & Ruiz, S. (2004). Decrease in glutathione content in boar sperm after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology. 62(3-4), 690-701.
Gunes, S., Hekim, G. N., Arslan, M. A. & Asci, R. 2016. Effects of aging on the male reproductive system. Journal of Assisted Reproduction and Genetics. 33, 441–454. doi: 10.1007/s10815-016-0663-y
Henry, M. & Neves, J. P. (2013). Manual para exame andrológico e avaliação de sêmen animal. CBRA, 2ed, 49p.
Hu, J. H.; Tian, W. Q.; Zhao, X. L.; Zan, L. S.; Wang, H.; Li, Q. W. & Xin, Y. P. (2010). The cryoprotective effects of ascorbic acid supplementation on bovine semen quality. Animal reproduction science. 121(1-2), 72-77.
Leite, T. G., Vale Filho, V. R., Arruda, R. P., Cesar, A. F., Emerick, L. L., Zaffalon, F. G., Martins, J. A. M. & Andrade, V. J. 2010. Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry. Animal Reproduction Science. 120, 31-38.
Melo, M. I. V.; Henry, M. & Beker, A. R. C. L. (2005). Teste hiposmótico para avaliação da viabilidade do sêmen eqüino resfriado com diferentes diluidores. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 57, 757-763.
Monteiro, J. C.; Gonçalves, J. S. A.; Rodrigues, J. A.; Lucio, C. F.; Silva, L. C. G.; Assumpção, M. E. O. A. & Vannucchi, C. I. (2009). Influence of ascorbic acid and glutathione antioxidants on frozen. Canine and feline reproduction IV, 44, 359-362.
Oliveira, R. A.; Wolf, C. A.; de Oliveira Viu, M. A. & Gambarini, M. L. (2013). Addition of glutathione to an extender for frozen equine semen. Journal of Equine Veterinary Science. 33(12), 1148-1152.
Pinto, S. C. C., Almeida, D. S., Alves, M., Florez-Rodriguez, S., Abreu Junior, G., Alves, N. B. E., Celeghini, E. C. C., Laskoski, L. M. & Souza, F. A. 2020. Does supplementation of vitamin C, reduced glutathione or their association in semen extender reduce oxidative stress in bovine frozen semen? Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 72, 9-17.
Ribeiro, V. H. A., Ribeiro, B. V., Silva, C. M. G., Martins, C. F., Abud, C. O. G. & Abud, L. C. (2022). Avaliação da qualidade do sêmen bovino criopreservado com diluidores de origem animal e vegetal. Brazilian Journal of Development. 8(10), 66182-66190.
Silva, P. F. N. & Gadella, B. M. (2006). Detection of damage in mammalian sperm cells. Theriogenology. 65(5), 958-978.
Silva, P. F.; Gadella, B. M.; Colenbrander, B. & Roelen, B. A. (2007). Exposure of bovine sperm to pro-oxidants impairs the developmental competence of the embryo after the first cleavage. Theriogenology. 67(3), 609-619.
Silva, E. C. B. & Guerra, M. M. P. (2012). Terapias antioxidantes na criopreservação espermática. Revista Portuguesa de Ciencias Veterinaria. 107, 143-9.
Ugur, M. R., Abdelrahman, A. S., Evans, H. C., Gilmore, A. A., Hitit, M., Arifiantini, R. I., Purwantara, B., Kaya, A. & Memili, E. (2019). Advances in Cryopreservation of Bull Sperm. Frontiers in Veterinary Science. 6(268), 1-15. doi:10.3389/fvets.2019.00268.
Valença, R. M. B. & Guerra, M. M. P. (2007). Espécies reativas ao oxigênio (ROS) e a utilização de antioxidantes na criopreservação do sêmen suíno. Revista Brasileira de Reprodução Animal. 31(1), 47-53.
Vianna, F. P., Papa, F. O., Zahn, F. S., Melo, C. M. & Dell'Aqua Junior, J. A. (2008) Thermoresistance sperm tests are not predictive of potential fertility for cryopreserved bull semen. Animal Reproduction Science, 113, 279-282.
Watson, P. F. (1995). Recent development and concepts in the cryopreservation of spermatozoa and the assessment of their post thawing function. Reproduction, Fertility and Development, 7, 871-891.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Fernando de Castro Parizi; Vanessa Balan Julio; Karine Kulik; Eduarda Stankiwich Vaz; Francielle Amorim da Silva Paes; Luciane Maria Laskoski; Marcos Vinicius Ferrari; Fernando Andrade Souza
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
