Physicochemical and hygienic-sanitary quality of honey marketed in Chókwè city

Authors

DOI:

https://doi.org/10.33448/rsd-v12i7.42360

Keywords:

Honey; Physicochemical analyses; Quality control.

Abstract

The present study aimed to evaluate the hygienic-sanitary conditions and the physical-chemical parameters of honey bees with emphasis on understanding the risks to health. The experiment was conducted in a completely randomized design with 5 samples, 3 from artisanal production (A, B and C) and 2 from industrial production (Youngs honey (D) and Goldcrest (E)). Analyzes such as titratable acidity by titration with 0.1N sodium hydroxide solution (NaOH), soluble solids content, refractometry, pH, potentiometry, humidity, gravimetry and ash by incineration were carried out for the physical-chemical characterization. The hygienic-sanitary characterization was performed for molds and yeasts using the Spread Plate method. Data were evaluated using the statistical software RStudio 4.2.1 at 5% significance of the Tukey test. The pH was fixed in the range of 3.28 to 4.30, soluble solids from 76.36 to 77.10 ºBrix, titratable acidity at the level of 4.14 to 6.10 meq/mL, moisture content of 12.07 to 17.12% and ash from 0.29 to 4.34%. The microbiological characterization showed the presence of molds in the range of 1.5x10 to 4.9x10. On the other hand, an uncountable load was verified in the yeast count in samples A and B, exceeding the limit established by the Technical Regulation of Quality and Identity of Honey. In terms of physical-chemical properties, no significant differences were found between artisanal and industrialized honey. The level of mold in all samples met recommended standards for honey and there was evidence of compliance with good manufacturing and hygienic-sanitary practices.

References

Abramovic, H. Jamnik, M. Burkan, L. & Kac, M. (2008). Water activity and water content in Slovenian honeys. Food Control, Guild ford. 19(11), 1086-90.

Albuquerque, J.C.G, Sobrinho, M.E, & Lins, T.C.L. (2021). Quality analysis of bee honey marketed with and without inspection in Brasília - DF, Brazil. Semina: Biological and Health Sciences, Londrina, 42(1), 71-80.

Almeida, D. (2002). Species of bees (Hymenoptera, Apoidea) and typification of honeys produced by them in a cerrado area in the municipality of Pirassununga. [Master's Thesis]. São Paulo.

Almeida-Anacleto, L. B. & Barion, F. (2007). Physicochemical evaluation of Brazilian honey from Jataí bee (Tetragonisca angustula). In: Apimondia International Apicultural Congress, 40, 2007, Melbourne, Australia. Proceedings. Melbourne, Australia: [s.n.]. p. 90-91.

Alves, E. M. (2008). Identification of the flora and characterization of organic honey honey and Africanized bees from the forest and orange islands of the upper Paraná River. [PhD thesis in zootechnics], State University of Maringá.

Alves, T. (2011). Physicochemical characterization and sensory evaluation of honeys produced by bees (Apis mellifera) from various blooms of the cariri region of Ceará. Green Magazine, Mossoró, 6(2). p.169 - 175.

Alvim, N. C. (2004). Honey and its characteristics. Electronic Scientific Journal of Veterinary Medicine - ISSN 1679-7353.

Andrade, I. C. S.; Maia, A. R. G.; Silva, M. G.; & Oliveira, S. C. P. (2019). Evaluation of mold and yeast contamination in honeys of apis mellifera l. from different regions. Bananeiras/PB.

Anvisa - National Health Surveillance Agency. (2022). Normative Instruction. In No. 161, dated July 1.

Aoac - Association of Official Analytical Chemists. 2016. Official Methods of Analysis of AOAC International Official Methods. 947.05. 20th edition. Rockville.

Araujo, D. R.; Silva, R. H. D.; & Sousa, J. S. (2006). Evaluation of the physicochemical quality of honey commercialized in the city of Crato, CE. Journal of Biology and Earth Sciences, Campina Grande.

Arruda, C. M. F. (2003). Physicochemical and pollen characteristics of honey samples of Apis mellifera L, (Hymenoptera, Apoidae) from the Chapada do Araripe region, municipality of Santana do Cariri. [Master's thesis]. Piracicaba.

Azeredo, L. C. Azeredo, M. A. A., Souza, S. R. & Dutra, V. M. L. (2003). Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, London, 80(2), 249-254.

Azevedo, M. A. A. Azevedo, L. D. A. C. & Damasceno, J. G. (2007). Physical and chemical characteristics of honey from Rio de Janeiro.

Barbosa, L. S. Macedo, J. L. Silva, M. R. F. & Machado, A. V. (2014). Biochemical study of the quality of bee honey commercialized in the municipality of Caraúbas – RN, Green Magazine of Agroecology and Sustainable Development.

Barth, M. O. Maiorino, C. Benatti, A. P. T. & Bastos, D. H. M. (2005). Determination of physicochemical parameters and botanical origin of indicated monofloral honeys in southeastern Brazil. Food Science and Technology, Campinas, 25(2), 229-233.

Bendini, J. N. & Souza, D. C. (2008). Physicochemical characterization of honey from bees from cashew flowering. Rural Science, Santa Maria, 38(2), 565-67.

Bertoldi, F. C. Gonzaga, L. & Reis, V. D. A. (2004). Physicochemical characteristics of honey from Africanized bees (Apismelli ferascutellata), with predominant flowering of field mint (Hyptiscrenata). produced in the Pantanal. In: Symposium on natural and socio-economic resources of the wetland. Corumbá, 1-4.

Brazil - Approves the Technical Regulation of Identity and Quality of Bee Honey. (2014). Rules approved by Decree No. 9,023 of March 15, 2014 and Art. 174, approved by Decree No. 15.

Campos, F. S. Gois, G. C. & Carneiro, G. G. (2010). Physicochemical parameters of honey from Melipona scutellaris bees produced in the state of Paraíba. FAZU in review, Uberaba, (7), 186-90.

Castro, L. O. Santos, C. C. Rebelo, T. R. Lopes, J. A. C. Viana, A. F. S. Abreu, A. S. Moreira, D. K. T. & Silva, B. A. (2022). Determination of physicochemical properties and melissopalynological constitution of Melipona (Michmelia) paraensis Ducke (Jandaíra) honey from Mojuí dos Campos – PA. Brazilian Development Magazine, Curitiba, 8(4), 23744-58.

Chaves, W. A. (2015). Quality of honey sold in free markets in the municipality of Teresina. Piaui. In: XXV Brazilian Congress of Animal Science. Fortaleza: ZOOTEC.

Damasceno, C. N. R. (2012). Physicochemical analysis of honey from bees commercialized in the municipality of Ariquemes/RO. Faculty of Education and Environment. [Monograph]. Ariquemes – RO.

Damto, T. Kebeba, D. & Gemeda, M. (2022). Physico-chemical and microbiological characteristics of honey produced by stingless bees (Meliponula beccarii) from the Oromia Region, Ethiopia. Available via license: CC BY 4.0. 10.1101/2022.12.23.521720

Dantas, J. D. Santos, S. C. L. Santos, T. C. L. Silva, A. B. & Carvalho, L. X. M. (2022). Physical-chemical analysis of bee honey sold in the city of Frei Martinho - PB. Research, Society and Development, 11(10), 3-7.

Denardi, C. A. S. Nishimoto, É. J. Balian, S. C. & Telles, E. O. (2005). Evaluation of water activity and contamination by molds and yeasts in honey marketed in the city of São Paulo-SP, Brazil. Journal of the Adolfo Lutz Institute (Printed), 219-222.

Dias, J. S. (2009). Physicochemical characterization of honey samples. Scientific Unopar: Exact and Technological Sciences, (8), 19-22.

Evangelista-Rodrigues, A., Silva, E. M. D., Beserra, E. M., & Rodrigues, M. L. (2005). Physicochemical analysis of honey bees (Apis mellifera and Melipona scutellaris) produced in different regions in the State of Paraíba. Rural Science, 35(5), 1166-71.

Farias, F. A. C. Monteiro, C. & Freitas, A. S. (2021). Evaluation of the physicochemical and microbiological quality of honeys commercialized in the city of São Luís, Maranhão.

Filho, J. P. A. Machado, A. V. Alves, F. M.S. Queiroga, K. H. & Candido, A. F. M. (2011). Physicochemical and quality study of bee honey commercialized in the municipality of Pombal – PB. Green Magazine. Mossoró - RN - Brazil, 6(3), 83.

Finco, F. D. B. A. Moura, L. L. & Silva, I. G. (2010). Physical and chemical properties of (Apis mellifera L) honey. Food Science and Technology, 30(3), 706-12.

Finola, M. S. Lasagno, M. C. & Marioli, J. M. (2007). Microbiological and chemical characterization of honeys from Central Argentina. Food Chemistry, 100(4), 1649-53.

Flangini, B. (2016). Bromatology of honey produced by apis mellifera marketed in the city of Rio Branco – Acre. [Master's Thesis]. Rio Branco – Acre.

Freitas, E. P. B. Craveiro, R. S. Lopes, Y. M. S. Assunção, J. M. B. & Souza, R. F. (2022). Physical-chemical characterization of honey produced in the municipality of Cachoeira do Arari - Ilha de Marajó, Pará. Research, Society and Development, 11(3), 1-19.

Gois, A. M. (2015). Analysis of physicochemical parameters of honeys produced and marketed by the settlement. Japaratuba.

Gois, G. C. Lima, C. A. B. Silva, L. T. & Envagelista-Rodrigues, A. (2013). Composition of honey (apis mellifera): quality requirements. Center for Agricultural Sciences, Campus II, Paraiba. Brazil. Veterinary Brasilica, 7(2).

Ial - Adolfo Lutz Institute. (2008). Physicochemical methods for food analysis. Edition: 4th ed. Sao Paulo.

Khan, F. R. Abadin, Z. U. & Rauf, N. (2007). Honey: nutritional and medicinal value. International Journal of Clinical Practice, v. 61, n. 10. p. 1705-1707.

Lima, M. G. L. Seraglio, S. K. T. Bergamo, G. Fett, R. & Costa, A. C. O. (2020). Identity and quality standards for apis mellifera honey. Ciagro-Brasil.

Mae – Ministry of state Administration. (2014). Profile of the Chókwè distrit. Gaza province. Avaliable at: http//:www.govnet.gov.mz.

Marchini, L. C. Sodre, G. D. S. Moreti, A. C. D. C. C. & Otsuk, I. P. (2004). Physicochemical composition of a sample of honeys from (Apis mellifera L.) from the State of Tocantins, Brazil. Animal Industry Bulletin, 61(2), 101-14.

Matos, I. T. S. R. Nunes, M. T. Mota, D. A. Laureano, M. M. M. & Hoshiba, M. A. (2012). Microbiological quality of honey (melipona sp). produced in the Central Amazon (Parintins–am–brazil), Green Magazine of Agroecology and Sustainable Development, 91-5.

Moraes, F. J. et al. (2014). Physicochemical characterization of Africanized bee honey samples from the municipalities of Santa Helena and Terra Roxa (PR). Arq. Bras. Med. Vet. Zootec., 66(4), 1269-75.

Okaneku, B. M. Souza, A. Q. L. Araújo, D. L. Alves, T. C. L. Cardoso, D. N. P. & Santos, W. G. (2020). Physicochemical and microbiological analysis of honey from Africanized bees (apis mellifera). Braz. J. of Develop., Curitiba, 6(4), 8607-20.

Oliveira, E. N. A. & Santos, D. C. (2011). Physicochemical analysis of honey from Africanized and native bees. Journal of the Adolfo Lutz Institute, 70(2), 132-38.

Parpinelli, R. S. (2016). Microbiological quality and physicochemical characterization of honey samples from stingless bees from six regions of the state of Paraná. [PhD thesis].

Pires, R. M. C. (2011). Quality of honey from Apis mellifera Linnaeus bees, produced in Piauí. [Master's Thesis in Food and Nutrition]. UFPI, Teresina-PI.

Ribeiro, R. & Starikoff, K. R. (2018). Evaluation of the physicochemical and microbiological quality of commercialized honey. Journal of Agroveterinary Sciences. Realeza, PR, Brazil.

Ritcher, W. (2011). Evaluation of the physicochemical quality of honey produced in the city of Pelotas/RS. Food and Nutrition Araraquara, (22), 547-553.

Rodrigues, F. M. Souza, F. G. & Rodrigues, L. G. S. M. (2012). Analysis of honey from small producers in the middle Araguaia-tocantins valley, Encyclopedia biosfera, knowing scientific center. Goiânia, 8 (15), 103.

Sant'ana, R. S. (2017). Physicochemical and microbiological characterization of melipona subnitida and melipona fasciculata honeys from the state of Piauí. [Master's thesis]. Bahia.

Santos, A. B. (2011). Determination of the authenticity of honeys sold in free markets and popular trades. Brazilian Educational Technology: research and learning. (2), 135-147.

Santos, A. C. Costa, A. C. O. Silva, B. Biluca, F. C. Braghini, F. Bergamo, G. Cella, I. Sattler, J. A. G. Gonzaga, L. V. Farias, M. Fruahauf, M. Cunha, R. D. & Seraglio, S. K. T. (2020). Quality of honey from honey Apis mellífera - good production and extraction practices. (Epagri. Boletim Didático, 148), 1st edition, Florianópolis, 76.

Santos, D. C. Martins, J. N. & Silva, K. F. N. L. (2010). Physicochemical and microbiological aspects of honey commercialized in the city of Tabuleiro do Norte-Ceará. Green Magazine, Mossoró, 5(1), 79 - 85.

Santos, W. B. (2018). Sensory of mead produced from honey from the semi-arid region of Piauí, 45.

Schmitz, B. Takahashi, S. E. Borba, L. P. Fluck, A. C. Costa, O. A. D. Foss, J. A. M. Maeda, E. M. & Araujo, D. N. (2022). Analysis of honey from different species of stingless bees. Zootechnics: current research – ISBN, Brazil, 4(1), 14-19.

Silva, A. A. N. (2022). Study of the quality of honey sold in the region of Barbacena - MG. Research, Society and Development, 11(10). 1-5.

Silva, A. C. Brito, M. G. A. Rocha, G. M. M. Sil, M. A. & Oliveira, G. A. L. (2021). Antimicrobial property and toxicity profile of iron-free honeys from the genus Melipona Illiger. Research, Society and Development, 10(4), page13510413903, 2021.

Silva, L. P. F. R. Rodrigues, M. A. S. Martins, S. S. Almeida, J. C. Melo, F. S. N. & Araujo, A. S. (2013). Verification of the microbiological quality of honeys produced and marketed in the Sertão Paraibano. Green Notebook of Agroecology and Sustainable Development, 3(2), 5.

Silva, M. B. L. (2007). Diagnosis of the production system and quality of apis mellifera honey. [Master’s thesis]. Viçosa.

Silva, R. A. Maia, G. A. Sousa, P. H. M. & Costa, G. M. C. (2006). Composition and therapeutic properties of bee honey. Alim. Nutr., Araraquara, 17(1), 113-20.

Silva, S. J. N. Schuch, P. Z. Vainstein, M. H. & Jablonsk, A. (2008). Determination of 5hydroxymethylfurfural in honeys using micellar capillary electrokinetic chromatography. Food Science and Technology, Campinas, 28, 46-50.

Sodré, G. S. Marchini, L. C. Moreti, A. C. C. C. Otsuk, I. P. & Carvalho, C. A. L. (2007). Physicochemical characterization of honey samples of Apis mellifera L. (Hymenoptera: Apidae) from the State of Ceará. Rural science, 37(4), 1139 - 44.

Sousa, J. M. B. Souza, E. L. Marques, G. Benassi, M. Gullón, B. Pintado, M. M. & Magnani, M. (2016). physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in brazilian semi-arid region. Food Science and Technology, 65, 645–51.

Souza, B. Roubik, D. Barth, O. M. Heard, T. Enriquez, E. Carvalho. C.Villas-Boas, J. Marchini, L. C. Locatelli, J. Oddo, L. P. Almeida-Muradian, L. B. Bogdanov, S. Vit, P. Souza, R. C. S. Yuyama, L. K. O. & Aguiar, J. P. L. (2006). Composition of stingless bee honey: Setting quality standards, Interciência. 31(12), 867-75.

Souza, C. F. Alves, L. R. P. Tulini, F. L. Mamede, A. G. N. Souza, D. M. Santana, A. C. B. A. & Lima, I. A. (2021). Analysis of honey samples sold in open-air markets in the city of Barreiras-Bahia. Conjecturas, 21(6), 428-442.

Souza, F. G. Rodrigues, F. M. & Rodrigues, L. G. S. M. (2012). Analsyis of honey from small producers in the Araguaia-Tocantis Middle Valley. Goiânia, 8(15), 101.

Souza, L. B. S. (2017). Physicochemical and microbiological characterization of honey from bees (apis mellifera) produced in the rural territory of identity Parque das emas – Goiás. [Master's thesis], Goiânia.

Vargas, T. (2006). Evaluation of the quality of honey produced in the region of Campos Gerais in Paraná. [Master's thesis], Ponta Grossa.

Vieira, T. R. Noguez, C. S. Santos, M. A & Wagner, S. A. (2023) when evaluating the physical-chemical and botanical characterization of stingless bee honey (Meliponini), occurring in the Taquari Valley - RS, for RTIQ edition. Research, Society and Development, 12(3), 1-14.

Welke, J. E. Reginatto, S. Ferreira, D. Vicenzi, R. & Soares, J. M. (2008). Physicochemical characterization of Apis mellifera L. honeys from the northwestern region of Rio Grande do Sul State. Rural Science, 38(6), 1737-741.

Wenzel, J. M. (2012). Evaluation of the microbiological quality of uninspected honey marketed in the city of Picos and macro-region of the State of Piauí. Peaks: UFPI, 28.

Downloads

Published

18/07/2023

How to Cite

MASSINGUE JÚNIOR, A. A. .; JOSÉ, A. E.; MACUÁCUA, T. J. Physicochemical and hygienic-sanitary quality of honey marketed in Chókwè city. Research, Society and Development, [S. l.], v. 12, n. 7, p. e8112742360, 2023. DOI: 10.33448/rsd-v12i7.42360. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/42360. Acesso em: 13 nov. 2024.

Issue

Section

Agrarian and Biological Sciences