The use of salivary biomarkers to monitor metabolic changes caused by physical exercise - A literature review

Authors

DOI:

https://doi.org/10.33448/rsd-v13i6.45943

Keywords:

Biomarkers; Saliva ; Monitoring physiologic ; Exercise.

Abstract

Introduction: Saliva is crucial for oral health, reflecting overall health due to its complex composition. With easy collection and a rich source of clinical information, saliva is promising for investigating physiological responses to exercise. Objective: To analyze the potential and limitations of salivary markers in monitoring biochemical changes induced by physical exercise. Methods: This literature review used databases like SciELO, PubMed, and Google Scholar with the keywords: "biomarkers," "saliva," "monitoring," and "physical exercise." A total of 272 studies were found. Inclusion criteria were: addressing the guiding question; original research and/or systematic reviews from the last 14 years; published in English, Portuguese, or Spanish; and having significant results. Ten studies were selected. Results: Salivary biomarkers were effective in assessing immune and endocrine status, monitoring oxidative stress and muscle damage, identifying sports concussions, and evaluating recovery methods' efficiency. Quantitative differences were observed between genders. The collection technique is simple, safe, inexpensive, and non-invasive but requires rigorous methodology and individual athlete analysis. Conclusion: Saliva is a rich source of biomarkers reflecting physiological responses to exercise, despite limitations like individual variability and the need for personalized monitoring.

References

Bellagambi, F. G., Lomonaco, T., Salvo, P., Vivaldi, F., Hangouët, M., Ghimenti, S., & Errachid, A. (2020). Saliva sampling: Methods and devices. An overview. TrAC Trends in Analytical Chemistry, 124, 115781.

Betteridge, D. J. (2000). What is oxidative stress? *Metabolism, 49*(2, Supplement 1), 3-8. https://doi.org/10.1016/S0026-0495(00)80077-3

Biagini, D., Lomonaco, T., Ghimenti, S., Fusi, J., Cerri, E., De Angelis, F., & Di Francesco, F. (2020). Saliva as a non-invasive tool for monitoring oxidative stress in swimmers athletes performing a VO2max cycle ergometer test. Talanta, 216, 120979.

Bispo, N. C., Gleeson, M. (2009) Efeitos agudos e crônicos do exercício nos marcadores de imunidade da mucosa. Frente. Biosci. 14, 4444–4456.

Chiappin, S., Antonelli, G., Gatti, R., & De Palo, E. F. (2007). Saliva specimen: A new laboratory tool for diagnostic and basic investigation. *Clinica Chimica Acta, 383*(1-2), 30-40. https://doi.org/10.1016/j.cca.2007.04.011

Chicharro, J.L., Lucia, A., Perez, M., Vaquero, A., & Urena, R. (1998) Composição e exercício da saliva. Esportes Med. 26, 17–27.

Damirchi, A., Kiani, M., Jafarian, V., & Sariri, R. (2010) Resposta da peroxidase salivar à intensidade do exercício. Eur. J. Appl. Físico., 108, 1233–1237.

Davison, G., Allgrove, J., Gleeson, M. Peptídeos antimicrobianos salivares (LL-37 e alfa-defensinas HNP1-3), respostas antimicrobianas e IgA para exercícios prolongados. Eur. J. Appl. Físico. 2009, 106, 277–284.

DustinSlivkaD. Slivka, WalterHailesW. Hailes, JohnCuddyJ. Cuddy, and BrentRubyB. Ruby. (2008) Caffeine and carbohydrate supplementation during exercise when in negative energy balance: effects on performance, metabolism, and salivary cortisol. Applied Physiology, Nutrition, and Metabolism. 33(6): 1079-1085.

Evans, L. W., & Omaye, S. T. (2017). Use of Saliva Biomarkers to Monitor Efficacy of Vitamin C in Exercise-Induced Oxidative Stress. Antioxidants (Basel, Switzerland), 6(1), 5. https://doi.org/10.3390/antiox6010005

Gillum, T., Kuennen, M., McKenna, Z., Castillo, M., Jordan-Patterson, A., Bohnert, C. O exercício não aumenta os linfócitos salivares, monócitos ou granulócitos, mas aumenta a lisozima salivar. J. Esportes de Ciência. 2017, 35, 1294–1299.

González Fernández, Á., de la Rubia Ortí, J. E., Franco-Martinez, L., Ceron, J. J., Mariscal, G., & Barrios, C. (2020). Changes in Salivary Levels of Creatine Kinase, Lactate Dehydrogenase, and Aspartate Aminotransferase after Playing Rugby Sevens: The Influence of Gender. International journal of environmental research and public health, 17(21), 8165. https://doi.org/10.3390/ijerph17218165

Guojian, H., Cuifeng G., Chenhong L. (2022). Refinement of saliva mi-RNA biomarkers for sport - Related concussion. Revista Brasileira Medicina do Esporte, 28(5). https://doi.org/10.1590/1517-8692202228052022_126

Hicks, S. D., Olympia, R. P., Onks, C., Kim, R. Y., Zhen, K. J., Fedorchak, G., DeVita, S., Rangnekar, A., Heller, M., Zwibel, H., et al. (2020) Biomarcadores de microRNA de saliva de concussão cumulativa. Int. J. Mol. Ciência, 21, 7758.

Huang, C. M. (2004). Comparative proteomic analysis of human whole saliva. Archives of Oral Biology, 49(12), 951-962. https://doi.org/10.1016/j.archoralbio.2004.06.003

Knab, A. M., Nieman, D. C., Gillitt, N. D., Shanely, R. A., Cialdella-Kam, L., Henson, D., & Meaney, M. P. (2014). Effects of a freeze-dried juice blend powder on exercise-induced inflammation, oxidative stress, and immune function in cyclists. Applied physiology, nutrition, and metabolism, 39(3), 381-385.

Levine, S. Influência das variáveis psicológicas na atividade do eixo hipotalâmico-hipófise-adrenal. Eur. J. Farmacol. 2000, 405, 149–160.

Ligtenberg, J.M., Marca, S., Petra, A.M., Veerman, C.I. O efeito do exercício físico na secreção salivar de MUC5B, amilase e lisozima. Arco. Biol Oral. 2015, 60, 1639–1644.

Mackinnon, L.T., Ginn, E., Seymour, G.J. Diminuição da taxa de secreção da imunoglobulina salivar A após exercícios de intervalo intenso em caiaques de elite. Eur. J. Appl. Físico. 1993, 67, 180–184

Mastaloudis, A., Leonard, S. W., & Traber, M. G. (2001). Oxidative stress in athletes during extreme endurance exercise. Free Radical Biology and Medicine, 31*(7), 911-922. https://doi.org/10.1016/S0891-5849(01)00667-0

Medina, S., Domínguez-Perles, R., Cejuela-Anta, R., Villaño, D., Martínez-Sanz, J. M., Gil, P., García-Viguera, C., Ferreres, F., Gil, J. I., & Gil-Izquierdo, A. (2012). Assessment of oxidative stress markers and prostaglandins after chronic training of triathletes. Prostaglandins & Other Lipid Mediators, 99(3-4), 79-86. https://doi.org/10.1016/j.prostaglandins.2012.07.002

Neves, R. S., da Silva, M. A. R., de Rezende, M. A. C., Caldo-Silva, A., Pinheiro, J., & Santos, A. M. C. (2023). Salivary Markers Responses in the Post-Exercise and Recovery Period: A Systematic Review. Sports (Basel, Switzerland), 11(7), 137. https://doi.org/10.3390/sports11070137

Nieman, D. C. (2009). Immune Function Responses to Ultramarathon Race Competition. Medicina Sportiva, 13(4), 189–196. https://doi.org/10.2478/v10036-009-0031-4

Ntovas, P., Loumprinis, N., Maniatakos, P., Margaritidi, L., & Rahiotis, C. (2022). The Effects of Physical Exercise on Saliva Composition: A Comprehensive Review. Dentistry Journal, 10(1), 7. https://doi.org/10.3390/dj10010007

Nunes, L. A. S., & Macedo, D. V. (2013). Saliva as a diagnostic fluid in sports medicine: potential and limitations. Jornal Brasileiro De Patologia E Medicina Laboratorial, 49(4), 247–255. https://doi.org/10.1590/S1676-24442013000400003

Ovchinnikov, A. N., Paoli, A., Seleznev, V. V., & Deryugina, A. V. (2022). Measurement of Lipid Peroxidation Products and Creatine Kinase in Blood Plasma and Saliva of Athletes at Rest and following Exercise. Journal of clinical medicine, 11(11), 3098. https://doi.org/10.3390/jcm11113098

Papacosta, E., & Nassis, G. P. (2011). Saliva as a tool for monitoring steroid, peptide and immune markers in sport and exercise science. Journal of science and medicine in sport, 14(5), 424–434. https://doi.org/10.1016/j.jsams.2011.03.004

Pilardeau, P., Richalet, J., Bouissou, P., Vaysse, J., Larmignat, P., & Boom, A. (1990) Fluxo e composição de saliva em humanos expostos à hipóxia de altitude aguda. Eur. J. Appl. Físico. Ocupar. Físico. 59, 450–453.

Rother, E. T. (2007). Revisão sistemática X revisão narrativa. Acta Paulista De Enfermagem, 20(2), v–vi. https://doi.org/10.1590/S0103-21002007000200001

Rutherfurd-Markwick, K., Starck, C., Dulson, D. K., & Ali, A. (2017). Salivary diagnostic markers in males and females during rest and exercise. Journal of the International Society of Sports Nutrition, 14, 27. https://doi.org/10.1186/s12970-017-0185-8

Sant'Anna, M. L., Oliveira, L. T., Gomes, D. V., Marques, S. T. F., Provance, D. W., Sorenson, M. M., & Salerno, V. P. (2019) O exercício físico estimula a secreção salivar de cistatinas. PLoS ONE. 14, e0224147.

Shimizu, K., Kimura, F., Akimoto, T., Akama, T., Kuno, S., & Kono, I. Efeito da atividade física diária de vida livre na IgA secretora salivar em idosos. Med. Ciência. Exercêno Esportivo. 2007, 39, 593–598.

Tanaka, Y., Naruishi, N., Nakayama, Y., Higashi, T., & Wakida, S.-i. (2006). Development of an analytical method using microchip capillary electrophoresis for the measurement of fluorescein-labeled salivary components in response to exercise stress. *Journal of Chromatography A, 1109*(2), 132-137. https://doi.org/10.1016/j.chroma.2005.11.081

Tiollier, E., Gomez-Merino, D., Burnat, P. et al. Treinamento intenso: imunidade das mucosas e incidência de infecções respiratórias. Eur J Appl Physiol 93, 421–428 (2005)an

Villiger, M., Stoop, R., Vetsch, T., Hohenauer, E., Pini, M., Clarys, P., Pereira, F., & Clijsen, R. (2018). Evaluation and review of body fluids saliva, sweat and tear compared to biochemical hydration assessment markers within blood and urine. Europejournal of clinical nutrition, 72(1), 69–76. https://doi.org/10.1038/ejcn.2017.136

Wu, A. J., & Ship, J. A. (1993). A characterization of major salivary gland flow rates in the presence of medications and systemic diseases. Oral Surgery, Oral Medicine, Oral Pathology, 76(3), 301-306. https://doi.org/10.1016/0030-4220(93)90258-6

Published

03/06/2024

How to Cite

CAMPOS, A. M. .; COSTA , G. V. A. M. .; LOPES , G. B. .; RODRIGUES, E. M. F. .; CASTRO , G. B. M. de . The use of salivary biomarkers to monitor metabolic changes caused by physical exercise - A literature review. Research, Society and Development, [S. l.], v. 13, n. 6, p. e1513645943, 2024. DOI: 10.33448/rsd-v13i6.45943. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/45943. Acesso em: 14 nov. 2024.

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Section

Health Sciences