Biodegradation of the carbofuran insecticide by Syncephalastrum racemosum

Authors

DOI:

https://doi.org/10.33448/rsd-v9i7.4932

Keywords:

Biodegradation; Carbofuran; Pesticides.

Abstract

Carbofuran (2.3-dihydro-2.2-dimethyl-benzofuranyl-N-methylcarbamate) is an insecticide belonging to the group of carbamates, widely used in pest control in crops with great commercial value, bioaccumulative and extremely toxic to the environment. An alternative to treat areas contaminated with pesticides is bioremediation, which consists of using microorganisms or their enzymes to remove persistent chemical compounds. Thus, the objective of this study was to determine the biodegradation of carbofuran, using the fungus Syncephalastrum racemosum, by evaluating fungal tolerance, enzymatic activity and quantifying residues by UV-Visible spectrophotometry. The results obtained showed that
S. racemosum presented best biodegradation capacity at 15 µg mL-1carbofuran concentration (93.02%), with IVCM values of 21.95 cm day-1, laccase (0.712 U mL-1) and lignin peroxidase (0.005 U mL-1), after 168 h incubation. Thus, the degradation rate in the 25 to 100 µg mL-1 range showed values of 12.66 to 75.58%, respectively. Based on these results, the fungus has great potential for use in the bioremediation, minimizing the impacts of this pesticide on the environment.

Author Biographies

Regiane Késsias de Sousa Lira, Universidade Federal do Rio de Janeiro

Programa de Pós-graduação em Tecnologia de Processos Químicos e Bioquímicos
Universidade Federal do Rio de Janeiro, UFRJ.

José Fábio França Orlanda, Universidade Estadual da Região Tocantina do Maranhão

Universidade Estadual da Região Tocantina do Maranhão, UEMASUL
Centro de Ciências Exatas, Naturais e Tecnológicas - CCENT
Laboratório de Biotecnologia Ambiental - LABITEC

References

Abdelhaleem, A., & Chu, W. (2019). Prediction of carbofuran degradation based on the hydroxyl radical’s generation using the Fe (III) impregnated N doped-TiO2/H2O2/Visible LED photo-fenton-like process. Chemical Engineering Journal, 382, 122930.

Agrofit. (2020). Sistema de consulta a agrotóxicos registrados no Brasil. Acesso em 22 maio, em http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons.

Castro-Gutierrez, V., Masís-Mora, M., Diez, M. C., Tortella, G. R., & Rodríguez-Rodríguez, C. E. (2017). Aging of biomixtures: effects on carbofuran removal and microbial community structure. Chemosphere, 168, 418-425.

Dangi, A. K., Sharma, B., Hill, R. T., & Shukla, P. (2019). Bioremediation through microbes: systems biology and metabolic engineering approach. Critical Reviews in Biotechnology, 39(1), 79-98.

Gomes, E. M. C. & Pena, R. da C. M. (2016). Isolamento, caracterização morfológica e avaliação do crescimento micelial e esporulação em diferentes meios de cultura de cepas do fungo Quambalaria sp. Biota Amazônia, 6(4), 59-63.

Ibrahim, K. E. A., & Şolpan, D. (2018). Removal of carbofuran in aqueous solution by using UV-irradiation/hydrogen peroxide. Journal of Environmental Chemical Engineering, 7(1), 102820.

Katembo, N., Witkowski, E. T., & Byrne, M. J. (2019). Effects of carbofuran on Lantana camara and its biocontrol agent, Teleonemia scrupulosa. Biocontrol Science and Technology, 29(8), 746-756.

Kaur, P., Balomajumder, C. (2019). Simultaneous biodegradation of mixture of carbamates by newly isolated Ascochyta sp. CBS 237.37. Ecotoxicology and Environmental Safety, 169, 590-599.

Kuwahara, M., Glenn, J. K., Morgan, M. A., & Gold, M. H. (1984). Separation and charaterisation of two extracellular H2O2 dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Letters, 169, 247-250.

Ma, L., Chen, S., Yuan, J., Yang, P., Liu, Y., & Stewart, K. (2017). Rapid biodegradation of atrazine by Ensifer sp. strain and its degradation genes. International Biodeterioration & Biodegradation, 116, 133-140.

Nie, J., Sun, Y., Zhou, Y., Kumar, M., Usman, M., Li, J., Shao, J.,Wang, L. & Tsang, D. C. W. (2019). Bioremediation of water containing pesticides by microalgae: mechanisms, methods, and prospects for future research. Science of The Total Environment, 707, 136080.

Nyakundi, W. O., Magoma, G., Ochora, J., & Nyende, A. B. (2012). Biodegradation of diazinon and methomyl pesticides by white rot fungi from selected horticultural farms in Rift Valley and Central Provinces, Kenya. In: Scientific Conference Proceedings. 639-654.

Popovska-Gorevski, M., Dubocovich, M. L., & Rajnarayanan, R. V. (2017). Carbamate insecticides target human melatonin receptors. Chemical research in toxicology, 30, 574-582.

Przystaś, W., Godlewska, E. Z., & Sota, E. G. (2018). Efficiency of decolorization of diffe rent dyes using fungal biomass immobilized on different solid supports. Environmental Microbiology, 49, 285-295.

Ruíz-Hidalgo, K., Masís-Mora, M., Barbieri, E., Carazo-Rojas, E., & Rodríguez-Rodríguez, C.E. (2016). Ecotoxicological analysis during the removal of carbofuran in fungal bioaugmented matrices. Chemosphere, 144, 864-871.

Salama, A. K. M. (1998). Metabolism of carbofuran by Aspegillus níger and Fusarium graminearum. Journal of Environmental Science and Health B, 33(3), 253-266.

Singh, R. K., Tripathi, R., Ranjan, A., & Srivastava, A. K. (2020). Fungi as potential candidates for bioremediation. Abatement of Environmental Pollutants. 177-191.

Szklarz, G. D., Antibus, R. K., Sinsabaugh, R. L., & Linkins, A. (1989). Production of phenoloxidases and peroxidases by wood-rootting fungi. Mycologia, 81, 234-240.

Tamrakar, U., Pillai, A. K., & Gupta, V. K. (2007). A simple colorimetric method for the determination of carbofuran and its application in environmental and biological samples. Journal of the Brazilian Chemical Society, 18(2), 337-341.

Tien, M., & Kirk, T. K. (1984). Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization and catalytic properties of a unique H2O2 requiring oxygenase. Proceedings of the National Academy of Sciences of the United States of America, 81(8), 2280-2284.

Downloads

Published

16/06/2020

How to Cite

LIRA, R. K. de S.; ORLANDA, J. F. F. Biodegradation of the carbofuran insecticide by Syncephalastrum racemosum. Research, Society and Development, [S. l.], v. 9, n. 7, p. e824974932, 2020. DOI: 10.33448/rsd-v9i7.4932. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/4932. Acesso em: 23 dec. 2024.

Issue

Vol. 9 No. 7

Section

Exact and Earth Sciences

License

Authors who publish with this journal agree to the following terms:

1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.