Evaluation of bioactive compounds from Sapodilla (Manilkara zapota) peel and seeds obtained by ultrasound-assisted technique
DOI:
https://doi.org/10.33448/rsd-v9i8.5158Keywords:
Phenolic; Flavonoid; Residue; Fruit.Abstract
Sapodilla is an exotic fruit consumed in several countries, which generates a significant amount of waste which can be used as a source of bioactive compounds. In this context, this work aimed to extract bioactive compounds from sapodilla peel and seeds through an ultrasound-assisted technique. This work is an explanatory quantitative research based on laboratory experiments. Extraction was carried out with distilled water, methanol and ethanol at concentrations between 40% and 80%, subjected to ultrasound for 1 h. The highest levels of phenolic compounds were obtained in 40% methanol peel extract (126.0 mg GAE/100 g of residue) and in 80% methanol seed extract (65.3 mg GAE/100 g of residue). In relation to total flavonoids, the highest levels were found in 80% ethanol peel extract (90.0 mg QCE/100 g) and in 80% ethanol seed extract (33.3 mg QCE/100g). The highest antioxidant activity for these extracts was obtained by the ABTS method, around 700.0 μM Trolox/g of residue. Seven polyphenolic compounds were identified and quantified by HPLC, with gallic acid being the major compound, followed by epigallocatechin and catechin. The ultrasound technique was efficient for obtaining bioactive extracts of sapodilla residues with potential for future application as a natural source of bioactive compounds.
References
Almeida, M. M. B., Sousa, P. H. M., Arriaga, A. M. C., Prado, G. M., Magalhães, C. E. C., Maia, G. A., & Lemos, T. L. G. (2011) Bioactive compounds and antioxidant activity of fresh exotic fruits from northeastern Brazil. Food Research International, 44, 2155–59.
Can-Cauich, C. A., Sauri-Duch, E., Betancur-Ancona, D., Chel-Guerrero, L., González-Aguilar, G. A., Cuevas-Glory, L. F., Pérez-Pacheco, E. & Moo-Huchin, V. M. (2017) Tropical fruit peel powders as functional ingredients: Evaluation of their bioactive compounds and antioxidant activity. Journal of Functional Foods, 37, 501–6.
Chemat, F., Huma, Z., & Khan, M.K. (2011) Applications of ultrasound in food technology: Processing, preservation and extraction. Ultrasonics Sonochemistry, 18, 813–35.
Costa, L. N., Freitas, W. E.S., Morais, P. L. D., Morais, D. L., & Mendonça, V. (2017) Efeito da adubação nitrogenada nos caracteres físico, físico-químico e potencial antioxidante do sapoti (Manilkara zapota L. P.Royen) em diferentes estádios de desenvolvimento. Acta Agronômica 66(4):480-5.
Deng, J.-S., Lee, S.-D., Kuo, W.-W., Fan, M.-J., Lin, Y.-M., Hu, W.-S., Huang, Y.-C., Velmurugan, B. K., Tsai, F.-J., Tsai C.-H., & Huang, C.-Y. (2014) Anti-apoptotic and pro-survival effect of protocatechuic acid on hypertensive hearts. Chemico-Biological Interactions, 209:77–4.
EFSA (2011).Scientific opinion on the evaluation of the substances currently on the list in the Annex to Commission Directive 96/3/EC as acceptable previous cargoes for edible fats and oils-Part I. III EFSA J. 9:47-0
Fernandes, F. H. A., & Salgado, H. R. N. (2016) Gallic Acid: Review of the Methods of Determination and Quantification. Critical Reviews in Analytical Chemistry, 46(3): 257–65.
Gomes, W. F., França, F. R. M., Denadai, M., Andrade, J. K. S., Oliveira, E. M. S., Brito, E. S., Rodrigues, S., & Narain, N. (2018) Effect of freeze- and spray-drying on physico-chemical characteristics, phenolic compounds and antioxidant activity of papaya pulp. Journal of Food Science and Technology, 6: 2095-02.
Gulçin, I. (2012) Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86:345–91.
Horincar, G., Enachi, E., Stănciuc, N. & Râpeanu, G. (2019) Extraction and characterization of bioactive compounds from eggplant peel using ultrasound – assisted extraction. Fascicle VI – Food Technology, 43:40-3.
Junior, J. F., Bezerra, J. E. F., Lederman, I. E., & Moura, R. J. M. (2014) O sapotizeiro no Brasil. Revista Brasileira de Fruticultura, 36:086-9.
Koçak, E., & Pazir F. (2018) Effect of Extraction Methods on Bioactive Compounds of Plant Origin. Turkish Journal of Agriculture - Food Science and Technology, 6(6): 663-75.
Kwon Y.I.I., Vattem D.A. & Shetty K. (2006) Evaluation of clonal herbs of Laminaceae species against diabetes and hypertension. Asia Pacific Journal of Clinical Nutrition, 15(1): 107-18.
Meda, A., Lamien, C. E., Romito, M., Millogo, J., & Nacoulma, O. G. (2005) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91:571–577.
Nenadis, N., Wang, L. F., Tsimidou, M., & Zhang, H. Y. (2004) Estimation of scavenging activity of phenolic compounds using the ABTS (*+) assay. Journal of Agricultural and Food Chemistry, 52:4669-74.
Oliveira, V. S., Afonso, M. R. A., & Da Costa, J. M. C. (2011) Caracterização físico-química e comportamento higroscópico de sapoti liofilizado. Revista Ciência Agronômica, 42(2), 342-48.
Pedro, A. C., Maciel, G. M., Ribeiro, V. R., & Haminiuk, C. W. I. (2019) Fundamental and applied aspects of catechins from diferente sources: a review. International Journal of Food Science and Technology, 55,429-42.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_MetodologiaPesquisa-Cientifica.pdf?sequence=1. Acesso em: 21 Abril 2020.
Rezende, Y. R. R., Nogueira, J. P., & Narain, N. (2017) Comparison and optimization of conventional and ultrasound assisted extraction for bioactive compounds and antioxidant activity from agro-industrial acerola (Malpighia emarginata DC) residue. LWT - Food Science and Technology 85:158-69.
Roubalova, L., Purchartova, K., Papouškova, B., Vacek, J., Kr, V., Ulrichova, J., & Vrba, J., 2015. Sulfation modulates the cell uptake, antiradical activity and biological effects of flavonoids in vitro: An examination of quercetin, isoquercitrin and taxifolin. Bioorganic & Medicinal Chemistry 23:5402–09.
Safdar, M. N., Kausar, T., Jabbar, S., Mumtaz, A., Ahad, K., & Saddozai, A. A. (2017) Extraction and quantification of polyphenols from kinnow (Citrus reticulate L.) peel using ultrasound and maceration techniques. Journal of food and drug analysis, 25:488-0.
Sancho, S. O., Silva, A. R. A., Dantas, A. N. S., Magalhaes, T. A., Lopes, G. S., Rodrigues, S., Costa, J. M. C., Fernandes, A. N., & Silva, M. G. V. (2015) Characterization of the Industrial Residues of Seven Fruits and Prospection of Their Potential Application as Food Supplements. Journal of Chemistry, 1-8.
Sharayeia, P., Azarpazhooha, E., Zomorodib, S., & Ramaswamyc, H. S. (2019) Ultrasound assisted extraction of bioactive compounds from pomegranate (Punica granatum L.) peel. LWT - Food Science and Technology, 101:342–0.
Shetty, K., Curtis, O. F., Levin, R. E., Witkowsky, R., & Ang, W. (1995) Prevention of verification associated with in vitro shoot culture of oregano (Origanum vulgare) by Pseudomonas spp. Journal of Plant Physiology, 147:447-1.
Silva, L.M.R., Figueiredo, E. A. T., Ricardo, N. M. P. S., Vieira, I. G. P., Figueiredo, R. W., Brasil, I. M., & Gomes, C. L. (2014) Quantification of bioactive compounds in pulps and by-products of tropical fruits from Brazil. Food Chemistry, 143:398–4.
Singh, J. P., Kaur, A., Shevkanil, K., & Singh, N. (2016) Composition, bioactive compounds and antioxidant activity of common Indian fruits and vegetables. Journal of Food Science and Technology, 53: 4056-6.
Snyder, L. R. (1974) Classification of the solvent properties of common liquids. Journal of Chromatography 92:233–0.
Tanaka, T., Onuma, H., Shigihara, T., Kimura, E., Fukuta, Y., Shirasaka, N., Moriyama, T., & Homma, Y. (2019) Anti-osteoporotic effects of syringic acid and vanilic acid in the extracts of waste beds after mushroom cultivation. Journal of Bioscience and Bioengineering, 128(5), 622-9.
Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L. & Byrne, D. H. (2006) Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, 19, 669–5.
Valvi, R. S., Rathod, V. S., & Yesane, D. P. (2011) Screening of three wild edible fruits for their antioxidant potential. Current Botany, 2,48-52.
Velayutham, P., Babu, A., & Liu, D. (2008) Green tea catechins and cardiovascular health: an update. Current Medicinal Chemistry, 15(18) 1840–50.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.