Comparison of endodontic disinfection of primary teeth root canals using rotary and reciprocating system: An in vitro study
DOI:
https://doi.org/10.33448/rsd-v9i8.5882Keywords:
Pediatric Dentistry; Primary teeth; Endodontics.Abstract
Aim: This experimental study in vitro aimed to compare the disinfection of the primary teeth root canals system using the rotary and reciprocating file system. Methodology: Forty-eight root canals from twenty-four primary molar teeth were contaminated with the standard strain of Enterococcus faecalis and randomically splitted into 4 groups (n=12). The Group PN comprised the root canals prepared with the ProTaperTM Next rotary system. The Group WOG was defined by the usage of WaveOneTM Gold reciprocating system. The files from both systems were thermo mechanical processed. The Group PU used the ProTaperTM Universal system, with no thermic treatment, and the Group C (negative control) received no treatment. Samples from the root canals were collected with sterile paper points before and after its preparation, diluted and distributed in plates with Brain Heart Infusion (BHI) agar. The bacterial colonies were counted and the results were statistic analyzed by Wilcoxon and Kruskal-Wallis (Dunn) tests. Results: There was significant bacterial reduction using the systems (p>0,01). However, there was no significant difference among them (p<0,05) (PN=3.38; WOG=3.85; PU=3.26). Conclusion: Both systems (rotary and reciprocating) provide disinfectionon the primary molar teeth root canals system.
References
Alves, F. R. F., Rôças, I. N., Almeida, B. M., Neves, M. A. S., Zoffoli, J., & Siqueira, J. F., (2012). Quantitative molecular and culture analyses of bacterial elimination in oval-shaped root canals by a single-file instrumentation technique. Int Endod J, 45(9), 871-7.
Barr, E. S., Kleier, D. J., & Barr, N. V. (2000) Use of nickel-titanium rotary files for root canal preparation in primary teeth. Ped Dent, 22(1), 77-8.
Bu ̈rklein, S., Hinschitza, K., Dammaschke, T., & Scha ̈fer, E. (2012). Shaping ability and cleaning effectiveness of two single-file systems in severely curved root canals of extracted teeth: Reciproc and WaveOne versus Mtwo and ProTaper. Int Endod J, 45(5), 449-61.
Card, S. J., Sigurdsson, A., & Orstavik, D., & Trope, M. (2002). The effectiveness of increased apical enlargement in reducing intracanal bacteria. J Endod, 28(11), 779-83.
Deshpande, A. N., Joshi, N. H., & Naik, K. S. (2017) In Vitro Comparative Evaluation of Cleaning Efficacy and Volumetric Filling in Primary Molars: Cone Beam Computed Tomography Evaluation. Contemp Clin Dent, 8(1), 33-7.
Elemam, R. F., Capelas, J. A., Vaz, M. A. P., Viriato, N., Pereira, M. L., Azevedo, A., & West, J. (2015). Evaluating Transportation by Comparing Several uses of Rotary Endodontic Files. J Contemp Dent Pract, 16(12), 927-32.
George, S., Anandaraj, S., Issac, J. S., John, S.A., & Harris, A. (2016). Rotary endodontics in primary teeth - A review. Saudi Dent J, 28(1), 12-7.
Gergi, R., Rjeily, J. A., Sader, J., & Naaman, A. (2010). Comparation of canal transportation and centering ability of twisted files, Pathfile-Protaper system, and stainless steel hand K-files by using compouted tomography. J Endod, 36, 904-7.
Gungor, O. E., & Kustarci, A. (2016). Evaluation of Apically Extruded Debris using Two Niti Systems Associated with Two İrrigation Techniques in Primary Teeth. J Clin Pediatr Dent, 40(6), 490-495.
Katge, F., Patil, D., Poojari, M., Pimpale, J., Shitoot, A., & Rusawat, B. (2014). Comparison of instrumentation time and cleaning efficacy of manual instrumentation, rotary systems and reciprocating systems in primary teeth: an in vitro study. J Indian Soc Pedod Prev Dent, 32(4), 311-6.
Krokidis, A., Bonfanti, C., Cerutti, A., Barabanti, N., Zinelis, S., & Panopoulos, P. (2017). Comparative analysis of SAF, Protaper Next and BT-Race in eliminating Enterococcus faecalis from long oval canals: An ex vivo study.Aust Endod J, 3, 1-5.
Machado, M. E. L., Nabeshima, C. K., Leonardo, M. F. P., Reis, F. A. S., Britto, M. L. B., & Cai, S. (2013). Influence of reciprocating single-file and rotary instrumentation on bacterial reduction on infected root canals. Int Endod J, 46(11), 1083-7.
Makarem, A., Ravandeh, N., & Ebrahimi, M. (2014). Radiografic assessment and chair time of rotatory instruments in the pulpectomy of primary second molar teeth: a randomized controlled clinical trial. J Dent Res Dent Clin Dent Prospects. 8, 84-9.
Moghaddam, K.N., Mehran, M., & Zadeh H.F. (2009). Root Canal Cleaning Efficacy of Rotary and Hand Files Instrumentation in Primary Molars. Int Endod J, 2(4), 53-7.
Musale, P. K., & Mujawar, S. A. (2014) Evaluation of the efficacy of rotary vs. hand files in root canal preparation of primary teeth in vitro using CBCT. Eur Arch Paediatr Dent, 15(2), 113-20.
Musani, I., Goyal V., Singh, A., & Bhat, C. (2009). Evaluation and comparison of biological cleaning efficacy of two endofiles and irrigants as judged by microbial quantification in primary teeth - an in vivo study. Int J Clin Pediatr Dent, 2(3), 15-22.
Neves, M. A. S., Provenzano, J. C., Rôças, I. N., & Siqueira, J. F. (2016) Clinical Antibacterial Effectiveness of Root Canal Preparation with Reciprocating Single-instrument or Continuously Rotating Multi-instrument Systems.J Endod, 42(1), 25-49.
Pinheiro, S. L., Araujo, G., Bincelli, I., Cunha, R., & Bueno, C. (2012) Evaluation of cleaning capacity and instrumentation time of manual, hybrid and rotary instrumentation techniques in primary molars. Int Endod J, 45(4), 379-85.
Pinheiro, S. L., da Silva, J. N., Gonçalves, R. O., & Villalpando K. T. (2014). Manual and Rotary Instrumentation Ability to Reduce Enterococcus faecalis Associated with Photodynamic Therapy in Deciduous Molars. Braz Dent J, 25(6), 502-7.
Pinheiro, S. L., Pessoa, C., da Silva, J. N., Gonçalves, R. O., Duarte, D. A., & da Silveira Bueno, C. E. (2016) Comparative Analysis of Protaper and Waveone Systems to Reduce Enterococcus Faecalis from Root Canal System in Primary Molars -An in Vitro Study. J Clin Pediatr Dent, 40(2), 124-8.
Prabhakar, A. R., Yavagal, C., Dixit, K., & Naik, S. V. (2016) Reciprocating vs Rotary Instrumentation in Pediatric Endodontics: Cone Beam Computed Tomographic Analysis of Deciduous Root Canals using Two Single-File Systems. Int J Clin Pediatr Dent, 9(1), 45-9.
Ramazani, N., Mohammadi, A., Amirabadi, F., Ramazani, M., & Ehsani, F. (2016) In vitro investigation of the cleaning efficacy, shaping ability, preparation time and file deformation of continuous rotary, reciprocating rotary and manual instrumentations in primary molars. J Dent Res Dent Clin Dent Prospects, 10(1), 49-56.
Ramezanali, F., Afkhami, F., Soleimani, A., Kharrazifard, M. J., & Rafiee, F. (2015). Comparison of Cleaning Efficacy and Instrumentation Time in Primary Molars: Two Rotary Instruments vs. Hand K-Files. Iran Endod J, 10(4), 240-3.
Schneider, S. W. (1971). A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol, 32(2), 271-5.
Siqueira, J. F., Lima, K. C., Magalhães, F. A., Lopes, H. P., & Uzeda, M. (1999) Mechanical reduction of the bacterial population in the root canal by three instrumentation techniques. J Endod, 25(5), 332-5.
Siqueira, J. F., Rôças, I. N., Santos, S. R., Lima, K. C., Magalhães, F. A., & Uzeda, M. (2002). Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals. J Endod, 28(3), 181-4.
Sowmya, H. K., Subhash, T. S., Goel, B. R., Nandini, T. N., Shilpa, H., & Bhandi, S. H. (2014). Quantitative assessment of apical debris extrusion and intracanal debris in the apical third, using hand instrumentation and three rotary instrumentation systems. J Clin Diagn Res, 8, 206–10.
Tewari, R. K., Ali, S., Mishra, S. K., Kumar, A., Andrabi, S.M., Zoya, A., & Alam, S. (2016) Mechanical reduction of the intracanal Enterococcus faecalis population by Hyflex CM, K3XF, ProTaper Next, and two manual instrument systems: an in vitro comparative study. J Investig Clin Dent, 7(2), 168-73.
Topçuoğlu, G., Topçuoğlu, H. S., & Akpek, F. (2016). Evaluation of apically extruded debris during root canal preparation in primary molar teeth using three different rotary systems and hand files. Int J Paediatr Dent, 26(5), 357-63.
Webber, J. (2015). Shaping canals with confidence: WaveOne GOLD single-file reciprocating system. Roots Int M Endod, 1, 34-40.
Yun, K. H., Lee, H. S., Nam, O. H., Moon, C. Y., Lee, J. H., & Choi, S. C. (2017). Analysis of bacterial community profiles of endodontically infected primary teeth using pyrosequencing. Int J Paediatr Dent, 27(1), 56-65.
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