Soybean inoculation techniques for the region of Tangará da Serra - MT, Brazil
DOI:
https://doi.org/10.33448/rsd-v9i8.6029Keywords:
Azospirillum brasilense; Bradyrhizobium japonicum; Inoculation; Co-inoculation; Re-inoculation.Abstract
The use of inoculated microorganisms on seeds has increased in Brazil, as they help on the fixation and use of nutrients by plants, promoting increasing yields, mainly due to the reduction in the application of nitrogen fertilizers. The aim of this study was to evaluate the influence of single inoculation and co-inoculation of Azospirillum brasilense and Bradyrhizobium japonicum bacteria on seeds and foliar inoculation on soybean, in the region of Tangará da Serra – MT, Brazil. The experimental design used was a randomized block with 9 treatments and 4 replicates. The treatments were composed by using B. japonicum and A. brasilense bacteria in different combinations and times of application on seeds and sprayed on the leaves. The use of A. brasilense together with B. japonicum significantly improved the agronomic characteristics of the evaluated soybean cultivar. There was an increase in yield with the use of A. brasilense through the co-inoculation technique when compared to the single use of Bradyrhizobium. The use of co-inoculation at V3 soybean stage promoted higher nodulation and yield than the standard inoculation performed in the seeds, ensuring better results of nodulation and yield besides increasing the size of the soybean root system, when there and co-inoculation techniques were implemented.
References
Bárbaro, I. M., Machado, P. C., Junior, B., Souza, L., Ticelli, M., Miguel F. B. & Silva, J. A. A. (2009). Soybean yield in response to standard inoculation and co-inoculation. Colloquium Agrariae, 5(1), 01-07. https://dx.doi.org/10.5747/ca.2009.v05.n1.a0040
Barzotto, F. (2015). Nitrogen and water as soybean productivity factors (Glycine max (L.) Merrill). (Master´s degree dissertation), Federal University of Santa Maria, Brazil. 59p.
Bottini, R., Fulchieri, M., Pearce, D. & Pharis, R. (1989). Identification of gibberelins A1, A3, and iso-A3 in cultures of A. lipoferum. Plant Physiology, 90(1), 45–47. https://dx.doi.org/10.1104%2Fpp.90.1.45
Braccini, A. L., Mariucci, G. E. G., Suzukawa, K. A., Lima, S. H. L. & Piccini, G. G. C. (2016). Soybean co-inoculation with Bradyrhizobium japonicum and Azospirillum brasilense and nitrogen fertilization on plant nodulation and crop yield. Scientia Agraria Paranaensis, 15(1), 27-35. http://dx.doi.org/10.18188/1983-1471/sap.v15n1p27-35
Bulegon, L. G. L., Rampim, L., Klein, J., Kestring, D., Guimarães, V. F., Battistus, A. G. & Inagaki, M. (2016). Components of production and yield of soybean inoculated with Bradyrhizobium and Azospirillum. Terra Latinoamericana, 34(2), 169-176.
Cacciari, I., Lippi, D., Pietrosanti, T. & Pietrosanti, W. (1989). Phytohormone-like substances produced by single and mixed diazotrophic cultures of Azospirillum and Arthrobacter. Plant and Soil, 115(1), 151-153. https://doi.org/10.1007/BF02220706
Câmara, G. M. S. (2000). Nitrogênio e produtividade da soja. In: Câmara, G. M. S. ed. Soja: Tecnologia da Produção II. Piracicaba: ESALQ/USP, 295-339.
Campos, B. C., Hungria, M. & Tedesco, V. (2001). Biological nitrogen fixation efficiency by strains of Bradyrhizobium in soybean under no-tillage. Revista Brasileira de Ciência do Solo, 25(3), 583-592.
Campos, B. H. C. & Gnatta, V. (2006). Inoculant and foliar fertilizer in soybean under no-tillage cultivated in areas with established Bradyrhizobium populations. Revista Brasileira de Ciência do Solo, 30(1), 69-76. https://doi.org/10.1590/S0100-06832001000300007
Dallacort, R., Martins, J. A., Inoue, M. H., Freitas, P. S. L. de & Coletti, A. J. (2011). Distribution of rainfall in the municipality of Tangará da Serra, medium north of the State of Mato Grosso, Brazil. Acta Scientiarum. Agronomy, 33(2), 193-200. https://doi.org/10.4025/actasciagron.v33i2.5838
Embrapa. Brazilian Agricultural Research Corporation. (2009). Manual de análises químicas de solos, plantas e fertilizantes. Rio de Janeiro: Embrapa Solos. 627p.
Embrapa. Brazilian Agricultural Research Corporation. (2013). Tecnologias de produção de soja na Região Central do Brasil, 2014: inoculação das sementes com Bradyrhizobium. Embrapa Soja, Londrina. 265p.
Embrapa. Brazilian Agricultural Research Corporation. (2017a). Brazilian system of soil classification. 5rd. ed. Embrapa Technological Information, Brasília. 356p.
Embrapa. Brazilian Agricultural Research Corporation. (2017b). Cultura de algodão no cerrado. 2th ed. Embrapa Algodão, Campina Grande. 73p.
Farias, J. R. B., Nepomuceno, A. L. & Neumaier, N. (2007). Ecofisiologia da soja. Brasília: Embrapa Informação Tecnológica. 353 p. (Circular técnica 48).
Ferreira, D. F. (2011). Sisvar: A computational system of statistical analysis. Ciência e Agrotecnologia, 35(6), 1039-1042. https://doi.org/10.1590/S1413-70542011000600001
Fipke, G. M. (2015). Co-inoculation and pre-inoculation of soybean seed. (Master´s degree dissertation), Federal University of Santa Maria, Brazil. 67p.
Huergo, L. F., Monteiro, R. A., Bonatto, A. C., Rigo, L. U., Steffens, M. B. R., Cruz, L. M., Chubatsu, L. S., Souza, E. M. & Pedrosa, F. O. (2008). Regulation of nitrogen fixation in Azospirillum brasilense. In: Cassán, F. D. & Salamone, G. (ed.) Azospirillum sp.: cell physiology, plant interactions and agronomic research in Argentina. 1th ed. Santa Fé: Asociación Argentina de Microbiologia, 17-28.
Hungria, M., Campo, R. J. & Mendes, I. C. (2001). Fixação biológica do nitrogênio na cultura da soja. Embrapa Soja, Londrina. 48p.
Hungria, M., Nishi, C. Y. M., Cohn, J. & Stace, G. (1996). Comparison between parental and variant soybean Bradyrhizobium strains with regard to the production of lipo-chitin nodulation signals, early stages of root infection, nodule occupancy, and N2 fixation rates. Plant and Soil, 186(2), 331-341. https://doi.org/10.1007/BF02415528
Hungria, M., Nogueira, M. A. & Araujo, R. S. (2013). Tecnologia de coinoculação da soja com Bradyrhizobium e Azospirillum: incrementos no rendimento com sustentabilidade e baixo custo. In: Reunião de pesquisa de soja da região central do Brasil. 33th ed. Brasília: Embrapa, 151-153.
Jameson, P. E. & Song, J. (2016). Cytokinin: a key driver of seed yield. Journal of Experimental Botany, 67(3), 593-606. https://doi.org/10.1093/jxb/erv461
Lazzarotto, J. J. & Hirakuri, M. H. (2010). Análises de correlações entre cotações internacionais do petróleo e de commodities agrícolas. In: Reunião de pesquisa de soja da região sul. 38th ed. Cruz Alta: Fundacep Fecotrigo, 1-4.
Lima, E. D. V., Crusciol, C. A. C., Cavariani, C. & Nakagawa, J. (2009). Agronomic traits, yield and physiological quality of “safrinha” (off-season) soybean under no till as a function of plant cover and surface liming. Revista Brasileira de Sementes, 31(1), 69-80. http://dx.doi.org/10.1590/S0101-31222009000100008
Marks, B. B., Megías, M., Nogueira, M. A. & Hungria, M. (2013). Biotechnological potential of rhizobial metabolites to enhance the performance of Bradyrhizobium spp. and Azospirillum brasilense inoculants with soybean and maize. AMB Express, 3(21), 01-10. https://doi.org/10.1186/2191-0855-3-21
Martins, L. M., Xavier, G. R., Range, F. W., Ribeiro, J. R. A., Neves, M. C. P., Morgado, L. B. & Rumjanek, N. G. (2003). Contribution of biological nitrogen fixation to cowpea: a strategy for improving grain yield in the semi-arid region of Brazil. Biology and Fertility of Soils, 38, 333-339. https://doi.org/10.1007/s00374-003-0668-4
Meert, L., Fernandes, F. B., Müller, M. M. L., Ary, D. & Rizzardi, J. D. S. E. (2020). Coinoculation with Bradyrhizobium japonicum and Azospirillum brasilense on soybean crop. Cultura Agronômica: Revista de Ciências Agronômicas, 29(1), 118-129.
Mendes, M. C., Rosário, J. G., Faria, M. V., Zocche, J. C. & Walter, A. L. (2011). Evaluation of agronomic efficiency of Azospirillum brasilense in wheat crop and the effects on flour quality. Applied Research & Agrotechnology, 4(3), 95-110.
Nonokawa, K., Nakajima, T., Nakamura, T. & Kokubun, M. (2012). Effect of synthetic cytokinin application on pod setting of individual florets within raceme in soybean. Plant Production Science, 15(2), 79-81. https://doi.org/10.1626/pps.15.79
Ormond, A. T. S., Voltarelli, M. A., Paixão, C. S. S., Gírio, L. A. S., Zerbato, C. & Silva, R. P. (2015). Agronomic characteristics of soybean under conventional and cross seeding. Revista Agro@mbiente On-line, 9(4), 414-422. http://dx.doi.org/10.18227/1982-8470ragro.v9i4.2706
Pereira, A. S., Shitsuka, D. M., Pereira, F. J. & Shitsuka, R. (2018). Methodology of cientific research. [e-Book]. UAB/NTE/UFSM, Santa Maria. Accessed on: Jul, 10th, 2020. Available at: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Reis Junior, F. B., Machado, C. T. T., Machado, A. T. & Sodek, L. (2008). Inoculation of Azospirillum amazonense in two maize genotypes under different nitrogen regimes. Revista Brasileira de Ciência do Solo, 32(3), 1139-1146.
Sousa, D. M. G. & Lobato, E. (2004). Cerrado: Correção do solo e adubação. 2th ed.: Embrapa Cerrados, Planaltina. 416p.
Souza, L. G. M. (2016). Otimização da fixação biológica de nitrogênio na soja em função da reinoculação em cobertura sob plantio direto. (Master´s degree dissertation), São Paulo State University Júlio de Mesquita Filho, Brazil. 45p.
Strzelczyk, E., Kamper, M. & Li, C. (1994). Cytocinin-like-substances and ethylene production by Azospirillum in media with different carbon sources. Microbiological Research, 149, 55-60. https://doi.org/10.1016/S0944-5013(11)80136-9
Tien, T. M., Gaskins, M. H. & Hubbell, D. H. (1979). Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Applied and Environmental Microbiology, 37(5), 1016-1024.
Vieira Neto, S. A., Pires, F. R., Menezes, C. C. E., Menezes, J. F. S., Silva, A. G., Silva, G. P. & Assis, R. L. (2008b). Forms of inoculant application and effects on soybean nodulation. Revista Brasileira de Ciência do Solo, 32(2), 861-870. https://doi.org/10.1590/S0100-06832008000200040
Vieira Neto, S. A., Pires, F. R., Menezes, C. C. E., Silva, A. G., Assis, R. L., Silva, G. P. & Menezes, J. F. S. (2008a). Forms of inoculant application and the effects in soybean crop. Bioscience Journal, 24(2), 56-68. https://doi.org/10.1590/S0100-06832008000200040
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Hugo Henrique Ribeiro Rosa, Rodrigo Ribeiro Fidelis, Alessandro Bandeira Dalbianco, Marcio Osvaldo Lima Magalhães, Luiz Henrique da Silva Barros, Roberto Antonio Savelli Martinez
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
