Chemical characterization and insecticidal potential of the essential oil of Ocimum gratissimum L. (Lamiaceae) against Nauphoeta cinerea (Blaberidae)

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.6412

Keywords:

Alfavaca-cravo; 1.8 Cineole; Biological activity; Bioinsecticide.

Abstract

Plants are considered a rich source of bioactive compounds and an alternative agent for insect control. Among them, representatives of the Lamiaceae family are the best known for this potential, as they produce volatile compounds with insecticidal activities, known as essential oils, as is the case with Ocimum gratissimum. In this way, the present work had as objective, to determine the chemical composition of the oil of the leaves of O. gratissimum and its potential insecticide against the cockroach Nauphoeta cinerea. For that, the essential oil was chemically characterized by means of a Gas Chromatograph coupled to a Mass Spectrometer (GC/MS). As for the insecticidal action, nymphs (20 days of age) were used and the fumigation methodology was used in concentrations ranging from 50 to 1000 μg of oil per mL of air. Mortality rates were observed every 12 hours for 1 day. The results showed that the essential oil has insecticidal properties, since it had a median lethal concentration (LC50) of 516 µg/mL. Such property may be related to the heterogeneous composition of the oil, which had 12 terpenic compounds, with 1.8 Cineole being the major compound (30.04%). Thus, the essential oil of O. gratissimum has an insecticidal activity and can be used to fight pests, as in the case of cockroaches.

References

Adedara, I. A., Rosemberg, D. B., Souza, D. O., Kamdem, J. P., Farombi, E. O., Aschner, M., & Rocha, J. B. (2015). Biochemical and behavioral deficits in the lobster cockroach Nauphoeta cinerea model of methylmercury exposure. Toxicology Research, 4(2), 442-451.

Adeniyi, S. A., Orjiekwe, C. L., Ehiagbonare, J. E., & Arimah, B. D. (2010). Preliminary phytochemical analysis and insecticidal activity of ethanolic extracts of four tropical plants (Vernonia amygdalina, Sida acuta, Ocimum gratissimum and Telfaria occidentalis) against beans weevil (Acanthscelides obtectus). International Journal of Physical Sciences, 5(6), 753-762.

Amirmohammadi, F., Sendi, J. J., & Zibaee, A. (2012). Toxicity and physiological effect of essential oil of Artemisia annua (Labiatae) on Agriolimax agrestis L.(Stylommatophora: Limacidae). Journal of Plant Protection Research, 52(2), 185-189.

Benelli, G., & Pavela, R. (2018). Repellence of essential oils and selected compounds against ticks—a systematic review. Acta Tropica, 179(s/n), 47-54.

Benelli, G., Pavela, R., Drenaggi, E., & Maggi, F. (2019a). Insecticidal efficacy of the essential oil of jambú (Acmella oleracea (L.) RK Jansen) cultivated in central Italy against filariasis mosquito vectors, houseflies and moth pests. Journal of Ethnopharmacology, 229(s/n), 272-279.

Benelli, G., Pavela, R., Maggi, F., Wandjou, J. G. N., Koné-Bamba, D., Sagratini, G., & Caprioli, G. (2019b). Insecticidal activity of the essential oil and polar extracts from Ocimum gratissimum grown in Ivory Coast: Efficacy on insect pests and vectors and impact on non-target species. Industrial Crops and Products, 132(s/n), 377-385.

Bett, P. K., Deng, A. L., Ogendo, J. O., Kariuki, S. T., Kamatenesi-Mugisha, M., Mihale, J. M., & Torto, B. (2016). Chemical composition of Cupressus lusitanica and Eucalyptus saligna leaf essential oils and bioactivity against major insect pests of stored food grains. Industrial Crops and Products, 82(s/n), 51-62.

Bezerra, J. W. A., Costa, A. R., de Freitas, M. A., Rodrigues, F. C., de Souza, M. A., da Silva, A. R. P., ... & Morais-Braga, M. F. B. (2019). Chemical composition, antimicrobial, modulator and antioxidant activity of essential oil of Dysphania ambrosioides (L.) Mosyakin & Clemants. Comparative immunology, microbiology and infectious diseases, 65(s/n), 58-64.

Bezerra, J. W. A., Costa, A. R., da Silva, M. A. P., Rocha, M. I., Boligon, A. A., da Rocha, J. B. T., ... & Kamdem, J. P. (2017). Chemical composition and toxicological evaluation of Hyptis suaveolens (L.) Poiteau (LAMIACEAE) in Drosophila melanogaster and Artemia salina. South African Journal of Botany, 113(s/n), 437-442.

Bezerra, J. W. A., Neto, F. A. G., Pereira Filho, J. T., de Alencar, G. R., Neto, J. C., Gonçalo, M. A. B. F., & Rocha, M. I. (2020). Chemical Composition and Insecticide Activity of Essential Oil of Mesosphaerum suaveolens Against Nauphoeta cinerea. Journal of Agricultural Studies, 8(2), 352-361.

Cruz, G. S., Wanderley-Teixeira, V., Silva, L. M., Dutra, K. A., Guedes, C. A., Oliveira, J. V., & Teixeira, Á. A. C. (2017). Chemical Composition and Insecticidal Activity of the Essential Oils of Foeniculum vulgare Mill., Ocimum basilicum L., Eucalyptus staigeriana F. Muell. ex Bailey, Eucalyptus citriodora Hook and Ocimum gratissimum L. and Their Major Components on Spodoptera frugiperda (Lepidoptera: Noctuidae). Journal of Essential Oil Bearing Plants, 20(5), 1360-1369.

Dhifi, W., Bellili, S., Jazi, S., Bahloul, N., & Mnif, W. (2016). Essential oils’ chemical characterization and investigation of some biological activities: A critical review. Medicines, 3(4), 25-41.

Duarte, A. E., De Menezes, I. R. A., Bezerra Morais Braga, M. F., Leite, N. F., Barros, L. M., Waczuk, E. P. & Kamdem, J. P. (2016). Antimicrobial activity and Modulatory effect of essential oil from the leaf of Rhaphiodon echinus (Nees & Mart) Schauer on some antimicrobial drugs. Molecules, 21(6), 743-757.

Giatropoulos, A., Kimbaris, A., Michaelakis, Α., Papachristos, D. P., Polissiou, M. G. & Emmanouel, N. (2018). Chemical composition and assessment of larvicidal and repellent capacity of 14 Lamiaceae essential oils against Aedes albopictus. Parasitology research, 117(6), 1953-1964.

Joshi, R. K. (2013). Chemical composition, in vitro antimicrobial and antioxidant activities of the essential oils of Ocimum gratissimum, O. sanctum and their major constituents. Indian journal of pharmaceutical sciences, 75(4), 457-462.

Kpoviessi, B. G. K., Kpoviessi, S. D., Ladekan, E. Y., Gbaguidi, F., Frédérich, M., Moudachirou, M. & Bero, J. (2014). In vitro antitrypanosomal and antiplasmodial activities of crude extracts and essential oils of Ocimum gratissimum Linn from Benin and influence of vegetative stage. Journal of ethnopharmacology, 155(3), 1417-1423.

Lima, A. S., Milhomem, M. N., Monteiro, O. S., Arruda, A. C. P., de Castro, J. A. M., Fernandes, Y. M. L. & Costa-Junior, L. M. (2018). Seasonal analysis and acaricidal activity of the thymol-type essential oil of Ocimum gratissimum and its major constituents against Rhipicephalus microplus (Acari: Ixodidae). Parasitology research, 117(1), 59-65.

Lisboa, C. F., Melo, E. C., Demuner, A. J., da Silva, L. C., Carneiro, A. P., & Coelho, A. P. F. (2020). Chemical composition of Lippia origanoides kunt. and Ocimum gratissimum L. essential oils stored at− 20° C. Industrial Crops and Products, 151(s/n),1-9.

Liška, A., Rozman, V., Kalinović, I., Eđed, A., Mustač, S., & Perhoč, B. (2011). Bioactivity of 1,8-cineole against red flour beetle Tribolium castaneum (Herbst). Poljoprivreda, 17(1), 58-63.

Lucia, A., Zerba, E., & Masuh, H. (2013). Knockdown and larvicidal activity of six monoterpenes against Aedes aegypti (Diptera: Culicidae) and their structure-activity relationships. Parasitology research, 112(12), 4267-4272.

Mawussi, G., Tounou, A. K., Ayisah, K. D., Vilarem, G., Raynaud, C., Merlina, G., ... & Sanda, K. (2012). Chemical composition and insecticidal activity of Ocimum canum essential oil against coffee berry borer, Hypothenemus hampei (Ferrari)(Coleoptera: Scolytidae). Journal of Essential Oil Bearing Plants, 15(6), 955-963.

Mith, H., Yayi-Ladékan, E., Sika Kpoviessi, S. D., Yaou Bokossa, I., Moudachirou, M., Daube, G., & Clinquart, A. (2016). Chemical composition and antimicrobial activity of essential oils of Ocimum basilicum, Ocimum canum and Ocimum gratissimum in function of harvesting time. Journal of Essential Oil Bearing Plants, 19(6), 1413-1425.

Mohr, F. B. M., Lermen, C., Gazim, Z. C., Gonçalves, J. E., & Alberton, O. (2017). Antifungal activity, yield, and composition of Ocimum gratissimum essential oil. Genetics and Molecular Research, 16(1), 1-10.

Pandey, S. (2017). Antibacterial and antifungal activities of Ocimum gratissimum L. Proteus, 9(12), 26-31.

Pandey, S., Singh, S. K., Kumar, N., & Manjhi, R. (2017). Antiviral, Antiprotozoal, Antimalarial and Insecticidal Activities of Ocimum gratissimum L. Asian Journal of Pharmaceutical Research and Development, 2(1), 1-9.

Park, C. G., Jang, M., Yoon, K. A. & Kim, J. (2016). Insecticidal and acetylcholinesterase inhibitory activities of Lamiaceae plant essential oils and their major components against Drosophila suzukii (Diptera: Drosophilidae). Industrial crops and products, 89(s/n), 507-513.

Pavela, R., Maggi, F., Lupidi, G., Mbuntcha, H., Woguem, V., Womeni, H. M. & Benelli, G. (2018). Clausena anisata and Dysphania ambrosioides essential oils: from ethno-medicine to modern uses as effective insecticides. Environmental Science and Pollution Research, 25(11), 10493-10503.

Shrestha, S., Poudel, A., Satyal, P., Dosoky, N. S., Chhetri, B. K., & Setzer, W. N. (2015). Chemical composition and biological activity of the leaf essential oil of Callistemon citrinus from Nepal. American Journal of Essential Oils and Natural Products, 2(5), 29-33.

Yeom, H. J., Lee, H. R., Lee, S. C., Lee, J. E., Seo, S. M., & Park, I. K. (2018). Insecticidal activity of lamiaceae plant essential oils and their constituents against Blattella germanica L. adult. Journal of Economic Entomology, 111(2), 653-661.

Published

11/08/2020

How to Cite

Rodrigues, F. C., Almeida-Bezerra, J. W., Fidelis, K. R., Costa, A. R., Souza, M. A. de, Costa, M. H. N. da, Santos, L. T. dos, Gonçalo, M. A. B. F. ., Menezes, S. A. de, Sousa, S. M. de, Araujo, E. K. R. de, Braga, A. V. P., Ribeiro, T. G., Costa, A. R. de S., Santos, M. A. F. dos, Nunes, R. G. dos S., & Rocha, M. I. (2020). Chemical characterization and insecticidal potential of the essential oil of Ocimum gratissimum L. (Lamiaceae) against Nauphoeta cinerea (Blaberidae). Research, Society and Development, 9(9), e72996412. https://doi.org/10.33448/rsd-v9i9.6412

Issue

Section

Agrarian and Biological Sciences