Characterization of silver nanoparticles and evaluation of their antimicrobial effect on Salmonella

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.6435

Keywords:

Silver nanoparticles; Antimicrobials; Food industry.

Abstract

Currently, the success of nanotechnology affects several areas of science, medicine, technology and, especially, the food industry. Silver nanoparticles (Ag-NPs) stand out, with their antimicrobial effect. Salmonella is a cause of foodborne diseases and there are reports of resistant serotypes. The use of Ag-NPs is an alternative for bacterial control in foods. The aim of this study was synthesize, characterize and verify the antimicrobial activity of Ag-NPs on serotypes of Salmonella. The  size of Ag-NPs was estimated and it was possible to detect two populations of 4.7 ± 0.09 and 35.7 ± 2.12. The zeta potential was -33.7 ± 11.8 mV indicating good dispersion stability.Ag-NP antimicrobial activity was determined from minimum inhibitory concentration (MIC).The lowest MIC found was 4.7 μg·mL-1 for Salmonella Enteritidis and the highest was 27.7 μg·mL-1 for Salmonella Infantis 1 isolate.The use of Ag-NPs is promising with respect to antimicrobial activity, however, improvements in synthesis methods should be explored in order to make commercial use viable.

References

Agnihotri, S., Mukherji, S., Mukherji, S. (2013). Size-controlled silver nanoparticles synthesized over the range 5–100 nm using the same protocol and their antibacterial efficacy. Royal Society of Chemistry, 4, 3974-3983. https://doi10.1039/C3RA44507K

Ardani, H.K., Imawan, C., Handayani, W., Djuhana, D., Harmoko, A., Fauzia, V. (2017). Enhancement of the stability of silver nanoparticles synthesized using aqueous extract of Diospyros discolor Willd. leaves using polyvinyl alcohol. In: IOP Conference Series: Materials Science and Engineering. IOP Publishing, 188, 12056. https://doi:10.1088/1757-899X/188/1/012056

Baker, C.; Pradhan, A.; Pakstis, L.; Pochan, D.J.; Shah, S.I. (2005). Synthesis and antibacterial properties of silver nanoparticles. Journal Nanoscience and Nanotechnology, 5, 244–249. https://doi.org/10.1166/jnn.2005.034

Bhattacharjee, S. DLS and zeta potential–What they are and what they are not?. Journal of Controlled Release, 235, p. 337-351, 2016. https://doi.org/10.1016/j.jconrel.2016.06.017

CDC. Centers for Disease Control and Prevention (2018). Reports of Selected Salmonella Outbreak Investigations, Salmonella Factsheet. Available at: https://www.cdc.gov/salmonella/pdf/CDC-Salmonella-Factsheet.pdf. Acessed on July 14, 2020.

CLSI . Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard . Ninth Edition. User Manual M07-A9, 32, n 2, p. 16 – 37, 2012.

Devi, G.K.; Kumar, K.S.; Parthiban, R.; Kalishwarlal, K. (2017). An insight study on HPTLC fingerprinting of Mukia maderaspatna: Mechanism of bioactive constituents in metal nanoparticle synthesis and its activity against human pathogens. Microbial pathogenesis, 102, 120-132. https://doi.org/10.1016/j.micpath.2016.11.026

FDA. Food and Drug Administration. (2015). Environmental Decision Memo for Food Contact Notification No. 1569, 2015. Available at: http://www.fda.gov/Food/IngredientsPackagingLabeling/EnvironmentalDecisions/ucm488455.htm. Acessed on July 14, 2020.

Flores, C.Y., Minan, A.G., Grillo, C.A., Salvarezza, R.C., Vericat, C., Schilardi, P.L. (2013). Citrate-capped silver nanoparticles showing good bactericidal effect against both planktonic and sessile bacteria and a low cytotoxicity to osteoblasticcells, ACS Applied Materials & Interfaces, 5, 3149–3159. https://doi.org/10.1021/am400044e

Guzman, M., Dille, J., Godet, S. (2012). Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine: Nanotechnology, Biology and Medicine, 8, 37-45. https://doi.org/10.1016/j.nano.2011.05.007

Losasso, C., Belluco, S., Cibin, V., Zavagnin, P., Micetic, I., Gallocchio, F., Zanella, M., Bregoli, L., Biancotto, G., RiccI, A. (2014) Antibacterial activity of silver nanoparticles: sensitivity of different Salmonella serovars. Frontiers in microbiology, 5. https://doi.org/10.3389/fmicb.2014.00227

Madigan M.T, Martinko J.M., Bender K.S., Buckley D.H., Stahl D.A. (2015) Brock Biology of Microorganisms. Glenview – IL: Pearson education. 1041.

Malvern Instruments Ltd. (2013) Zeta Potential Theory. Zetasizer Nano Series UserManual Man0485. Available at: https://www.chem.uci.edu/~dmitryf/manuals/Malvern%20Zetasizer%20ZS%20DLS%20user%20manual.pdf. Acessed on July 14, 2020.

Martínez-Castañón, G.A., Niño-Martínez, N., Martínez-Gutierrez, F. (2008). Martínez-Mendoza, J.R., Ruiz, F. Synthesis and antibacterial activity of silver nanoparticles with different sizes. Journal of Nanoparticle Research, 10, 1343-1348. https://doi.org/10.1007/s11051-008-9428-6.

Morones, J., Elechiguerra, J., Camacho, A., Ramirez, J. (2005). The bactericidal effect of silver nanoparticles. Nanotechnology , 16, 2346–53.

Nobbmann, U. (2015). Malvern Panalytical. Material Talks. PDI from an individual peak in DLS. Available at: < http://www.materials-talks.com/blog/2015/03/31/pdi-from-an-individual-peak-in-dls/>. Acessed on July 14, 2020.

Nobbmann, U., Morfesis, A. (2009). Light scattering and nanoparticles. Materials today, 12, 52-54. https://doi.org/10.1016/S1369-7021(09)70164-6

Omara, S.T.; Zawrah, M.F.; Samy, A.A. (2017). Minimum bactericidal concentration of chemically synthesized silver nanoparticles against pathogenic Salmonella and Shigella strains isolated from layer poultry farms. Journal of Applied Pharmaceutical Science, 7, 214-221. https://doi:10.7324/JAPS.2017.70829

Padmos, J.D., Boudreau, R., Weaver, D.F., Zhang, P. (2015). Impact of protecting ligands on surface structure and antibacterial activity of silver nanoparticles. Langmuir, 31, 3745-3752. https://doi.org/10.1021/acs.langmuir.5b00049

Panacék, A., Kvitek, L., Prucek, R., Kolar, M., Vecerova, R., Pizurova, N., Virender, K., Nevecna, T., Zboril, R. (2006). Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. The Journal of Physical Chemistry B, 110, 16248–53. https://doi.org/10.1021/jp063826h

Peng, D. (2016). Biofilm Formation of Salmonella. In: Dhanasekaran, D., Thajuddin. Microbial Biofilms – Importance and Applications. Intech open science, p. 231-249.

Pereira AS et al (2018). Methodology of cientific research. [e-Book]. Santa Maria City. UAB / NTE / UFSM Editors. Available at: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1. Acessed on July 14, 2020.

Perkin Elmer. The 30-Minute Guide to ICP-MS - ICP-Mass Spectrometry. Available at: https://www.perkinelmer.com/CMSResources/Images/44-74849tch_icpmsthirtyminuteguide.pdf. Acessed on July 17, 2020.

Pinzaru, I., Coricovac, D., Dehelean, C., Moacă, E.A., Mioc, M., Baderca, F., Sizemore, I., Brittle, S., Marti, D., Calina, C.D., Tsatsakis, A.M., Soica, C. (2018). Stable PEG-coated silver nanoparticles–A comprehensive toxicological profile. Food and Chemical Toxicology, 111, 546-556. https://doi.org/10.1016/j.fct.2017.11.051

Prema P., Thangapandiyan, S., Immanuel, G. (2017). CMC stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. Carbohydrate polymers, 158, 141-148. https://doi.org/10.1016/j.carbpol.2016.11.083

Pui, C.F., Wong, W.C., Chai, L.C., Lee, H.Y., Tang, J.Y.H., Noorlis, A., Farinazleen, M.G., CHEAH, Y.K., SON, R. (2011). Biofilm formation by Salmonella Typhi and Salmonella Typhimurium on plastic cutting board and its transfer to dragon fruit. International Food Research Journal, 18, 31-38.

Raman, G., Park, S.J., SakthiveL, N., Suresh, A.K. (2017). Physico-cultural parameters during AgNPs biotransformation with bactericidal activity against human pathogens. Enzyme and Microbial Technology, 100, 45-51. https://doi.org/10.1016/j.enzmictec.2017.02.002

Rogers, K.R., Navratilova, J., Stefaniak, A., Bowers, L., Knepp, A. K., Al-abed, S.R., Potter, P., Gitipour, A., Radwan, I., Nelson, C., Bradham, K.D. (2018). Characterization of engineered nanoparticles in commercially available spray disinfectant products advertised to contain colloidal silver. Science of The Total Environment, 619, 1375-1384. https://doi.org/10.1016/j.scitotenv.2017.11.195

Sadowski, Z., Maliszewska, I.H., Grochowalska, B., Polowczyk, I., Koźlecki, T. (2008). Synthesis of silver nanoparticles using microorganisms. Material Science-Poland, 26, 2- 6.

Solomon, S.D.; Bahadory, M.; Jeyarajasingam, A.V.; Rutkowsky, S.A.; Boritz, C. (2007). Synthesis and study of silver nanoparticles. Journal of Chemical Education, 84, 322. https://doi.org/10.1021/ed084p322

Sosa, I.O., Noguez, C., Barrera, R.G. (2003). Optical properties of metal nanoparticles with arbitrary shapes. The Journal of Physical Chemistry B, 107, 6269-6275. https://doi.org/10.1021/jp0274076

Sotiriou, G.A., Meyer, A., Knijnenburg, J.T.N., Panke, S., Pratsinis, S.E. (2012). Quantifying the origin of released Ag+ ions from nanosilver. Langmuir, 28, 15929-15936. https://doi.org/10.1021/la303370d

Steenackers, H., Hermans, K., Vanderleyden, J., Keersmaecker, S.C.J.D. (2012). Salmonella biofilms: an overview on occurrence, structure, regulation and eradication. Food Research International, 45, 502-531.

Surmeneva, M.A., Sharonova, A.A., Chernousova, S., Prymak, O., Loza, K., Tkachev, M.S., Shulepov, I.A., Epple, M., Surmenev, R.A. (2017). Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating. Colloids and Surfaces B: Biointerfaces, 156, 104-113. https://doi.org/10.1016/j.colsurfb.2017.05.016

Turkevich, J., Stevenson, P.C., Hillier, J. A study of the nucleation and growth processes in the synthesis of colloidal gold. Discussions of the Faraday Society, v. 11, p. 55-75, 1951.

Wagner, C., Hensel, M. Adhesive Mechanisms of Salmonella enterica. (2011). In: LInke, D.; Goldman, A. Bacterial Adhesion, Advances in Experimental Medicine and Biology. Germany. Springer Science Business, 17-34.

Williams, K., Valencia, L., Gokulan, K., Trbojevich, R., Khare, S. (2017). Assessment of antimicrobial effects of food contact materials containing silver on growth of Salmonella Typhimurium. Food and Chemical Toxicology, 100, 197-206. https://doi.org/10.1016/j.fct.2016.12.014

Wolf, R.E. (2005). What is ICP-MS? Available at: <https://crustal.usgs.gov/laboratories/icpms/What_is_ICPMS.pdf>. Acessed on July 14, 2020.

Zarei, M., Jamnejad, A., Khajehali, E. (2014). Antibacterial effect of silver nanoparticles against four foodborne pathogens. Jundishapur Journal of Microbiology, 7. https://doi: 10.5812/jjm.8720

Zhang, H., Chen, G. (2009). Potent antibacterial activities of Ag/TiO2 nanocomposite powders synthesized by a one-pot sol gel method. Environmental Science & Technology, 43, 2905-2910. https://doi.org/10.1021/es803450f

Zhang, W.; Yao, Y.; Sullivan, N.; Chen, Y. (2011). Modeling the primary size effects of citrate-coated silver nanoparticles on their ion release kinetics. Environmental Science & Technology, 45, 4422-4428. https://doi.org/10.1021/es104205a

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Published

13/08/2020

How to Cite

SOUZA, L. V.; LEAL, D. A.; COSTA, T. R. da; MENDONÇA, R. C. S. Characterization of silver nanoparticles and evaluation of their antimicrobial effect on Salmonella. Research, Society and Development, [S. l.], v. 9, n. 9, p. e103996435, 2020. DOI: 10.33448/rsd-v9i9.6435. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/6435. Acesso em: 22 nov. 2024.

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Agrarian and Biological Sciences