Comparative study of cryopreservation techniques in somatic tissue of Felis silvestris catus

Authors

DOI:

https://doi.org/10.33448/rsd-v9i8.6686

Keywords:

Felids; Tissue biobanks; Histology.

Abstract

The aim of this study was to compare the effects of Slow Freezing (SF) and Solid-Surface Vitrification (SSV) on the cryopreservation of the apical ear skin of domestic cats using histological tools. Fragments of skin obtained from six cats (Felis silvestres catus) were processed and destined for cryopreservation by SF and SSV, using a solution composed of Dulbecco’s Modified Eagle Medium (DMEM) plus dimethyl sulfoxide (DMSO) at 1.5 M or 3.0 M, for SF and SSV, respectively, in addition to 0.25 M sucrose and 10% fetal bovine serum (FBS). After warming, the samples were destined for histological processing for subsequent staining with hematoxylin-eosin, Gromori trichrome, and Weigert staining, as well as the marking of nucleolus organizing regions (NORs) using silver nitrate. Thus, using the VSS technique, characteristics such as epidermis thickness, dermis, and total skin thickness, presented values similar to the control group (p > 0.05). The VSS technique proved to be efficient in maintaining the number of epidermal cells and the percentage of collagen fibers. It is concluded that the application of the VSS technique proved to be superior in the histological maintenance of the apical auricular skin of domestic cats, assisting in the generation of a somatic bank in the species.

References

Amadeu, T. P., Coulomb, B., Desmouliere, A., & Costa, A. M. A. (2003). Cutaneous wound healing: myofibroblastic differentiation and in vitro models. The International Journal of Lower Extremity Wounds, 2(2), 60-68.

Borges, A. A., Lima, G. L., Queiroz Neta, L. B., Santos, M. V. O., Oliveira, M. F., Silva, A. R., & Pereira, A. F. (2017) Conservation of somatic tissue derived from collared peccaries (Pecari tajacu Linnaeus, 1758) using direct or solid-surface vitrification techniques. Cytotechnology, 69, 643-654.

Borges, A. A., Queiroz Neta, L. B., Santos, M. V. O., Oliveira, M. F., Silva, A. R., & Pereira, A. F. (2018). Combination of ethylene glycol with sucrose increases survival rate after vitrification of somatic tissue of collared peccaries (Pecari tajacu Linnaeus, 1758). Pesquisa Veterinária Brasileira, 38, 350-356.

Davies, O. G., Smith, A. J., Cooper, P. R., Shelton, R. M., & Scheven, B. A. (2014). The effects of cryopreservation on cells isolated from adipose, bone marrow and dental pulp tissues. Cryobiology, 69(2), 342-347.

Cheuquemán, C., Faúndez, R., Sánchez, R., & Risopatrón, J. (2018). Changes in sperm function and structure after freezing in domestic cat spermatozoa. Andrologia, 50(9), e.13080.

Grisolio, A. P. R., Picinato, M. A. C., Nunes, J. O. R., & Carvalho, A. A. B. (2017). O comportamento de cães e gatos: sua importância para saúde pública. Revista de Ciência Veterinária e Saúde Pública, 4(1), 117-126.

Jang, T. H., Park, S. C., Yang, J. H., Kim, J. Y., Seok, J. H., Park, U. S., Choi, C. W., Lee, S. R., & Han, J. (2017) Cryopreservation and its clinical applications. Integrative Medicine Research, 6, 12-18.

Khiao, I. M., Richardson, K. C., Loewa, A., Hedtrich, S., Kaessmeyer, S., & Plendl, J. (2019). Histological and functional comparisons of four anatomical regions of porcine skin with human abdominal skin. Anatomia, Histologia, Embryologia, 48, 207-217.

Lima, D. B. C., & Silva, M. D. L. (2018). Vitrificação testicular de felinos pré-púberes. Revista Brasileira de Reprodução Animal, 42, 125-128.

Luvoni, G. C., & Colombo, M. (2020). Cold case: Small animal gametes cryobanking. Theriogenology, 150, 445-451.

Mochiduki, Y., & Okita, K. (2012). Methods for iPS cell generation for basic research and clinical applications. Biotechnology Journal, 7, 789-797.

Praxedes, É. A., Borges, A. A., Santos, M. V. O., & Pereira, A. F. (2018) Use of somatic cell banks in the conservation of wild felids. Zoo Biology, 37(4), 258-263.

Praxedes, É. A., Oliveira, L. R. M., Silva, M. B., Borges, A. A., Santos, M. V. O., Silva, H. V. R., Oliveira, M. F., Silva, A. R., & Pereira, A. F. (2019) Effects of cryopreservation techniques on the preservation of ear skin – An alternative approach to conservation of jaguar, Panthera onca (Linnaeus, 1758). Cryobiology, 88, 15-22.

Praxedes, É. A., Queiroz Neta, L. B, Borges, A. A., Silva, M. B.; Santos, M. V. O., Ribeiro, L. R., Silva, H. V. R., & Pereira, A. F. (2020). Quantitative and descriptive histological aspects of jaguar (Panthera onca Linnaeus, 1758) ear skin as a step towards formation of biobanks. Anatomia, Histologia, Embryologia, 49(1), 121-129.

Preradovic, L.F., Danic, D., Kardum-skelin, I., Sarcevic, B., & Hadzibegovic, A.D. (2017). AgNOR Counts in differential diagnosis of parathyroid adenoma and hyperplasia in preoperative cytologic smears. Southeastern European Medical Journal, 1, 19-26.

Queiroz Neta, L. B., Lira, G. P. O., Borges, A. A., Santos, M. V. O., Silva, M. B., Oliveira, L. R. M., Silva, A. R., Oliveira M. F., & Pereira, A. F. (2018). Influence of storage time and nutrient medium on recovery of fibroblast-like cells from refrigerated collared peccary (Pecari tajacu Linnaeus, 1758) skin. In Vitro Cellular & Developmental Biology-Animal, 54(7), 486-495.

Santos, M. D. C. B. (2019). Avaliação histológica da pele auricular, femoral e caudal de onça-pintada, Panthera onca (Linnaeus, 1758) e sua qualidade após a vitrificação. Universidade Federal Rural do Semiárido (Graduação em Biotecnologia).

Souza, L. C., Pinheiro Junior, O. Á., Mendonça A. O., & Ballaris, A. L. (2001). Associação homem-animal: reflexos na economia. Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP, 4(1), 62-65.

Souza, T. M., Fighera, R. A., Kommers, G. D., & Barros, C. S. (2009). Aspectos histológicos da pele de cães e gatos como ferramenta para dermatopatologia. Pesquisa Veterinária Brasileira, 29, 177-190.

Vidane, A., Pinheiro A., Casals J., Passarelli, D., Hage, M., Bueno, R., Martins, D., & Ambrosio, C. (2017). Transplantation of amniotic membrane-derived multipotent cells ameliorates and delays the progression of chronic kidney disease in cats. Reproduction in Domestic Animals, 52, 316-326.

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Published

06/08/2020

How to Cite

MARTINS, J. C. J.; PRAXEDES, Érika A.; NASCIMENTO, M. B. do; AQUINO, L. V. C. de; ALVES, N. D.; OLIVEIRA, M. F. de; PEREIRA, A. F. Comparative study of cryopreservation techniques in somatic tissue of Felis silvestris catus . Research, Society and Development, [S. l.], v. 9, n. 8, p. e969986686, 2020. DOI: 10.33448/rsd-v9i8.6686. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/6686. Acesso em: 5 nov. 2024.

Issue

Vol. 9 No. 8

Section

Agrarian and Biological Sciences

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Copyright (c) 2020 Alexsandra Fernandes Pereira, Jane Cleide Jenuario Martins, Érika Almeida Praxedes, Matheus Barbosa do Nascimento, Leonardo Vitorino Costa de Aquino, Nilza Dutra Alves, Moacir Franco de Oliveira

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