Bacteriotech construction in a higher education institution for teaching and research purposes

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.7101

Keywords:

Bacteriotech; Cryopreservation; Teaching; Research.

Abstract

Introduction: Bacteriotecs have one for great relevance to Higher Education Institutions, both for didactic use and for use in research. The storage methods aim to preserve the morphophysiological aspects of the specimens for use at any time. Microbial conservation techniques can be short term, medium term and long term. Among the principles of each technique are cryopreservation methods with common freezing and deep-freezing associated with cryoprotective additives to reduce and protect freeze-related damage. Over the years, the construction of the bacteotecs has been consolidated by providing bacterial bioavailability for educational and research purposes. Objective: This research aimed at building the bacteriotech for the Microbiology Laboratory of the Higher Education Unit of Feira de Santana. Methodology: 14 bacteria were introduced during the research, applying the storage by freezing at -20° C with 20% glycerol and 15% skimmed milk in TSB broth, in addition to the management for maintenance, feasibility and quality control performed in parallel. Results: The technique adopted for cryopreservation in the medium term with the use of two cryoprotectors, as well as the procedures adopted for viability, were able to guarantee for 1 year and 4 months the purity of the available strains without loss of viability. Conclusion: It was noted that the insertion of the bacteriotech was able to meet the academic and scientific needs of the Institution, so the bacteriotech can be inserted with easy and low-cost techniques.

References

Abreu, M. M. V., & Tutunji, V. L. (2004). Implantação e manutenção da coleção de culturas de microorganismos do UniCEUB. Universitas: Ciências da Saúde (Brasília), 2 (2), 236-251.

Carvalho, A. S., Silva, J., Ho, P., Teixeira, P., Malcata, F. X., & Gibbs, P. (2004). Effects of various sugars added to growth and drying media upon thermotolerance and survival throughout storage of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus. Biotechnol Prog. 20 (1), 248-254.

Costa, C. P., & Ferreira, M. C. (1991). Preservação de microrganismos: revisão. Revistade Microbiologia, (São Paulo), 22 (3), 263-268.

Hubálel, Z. (2003). Protectants used in the cryopreservation of microorganisms. Cryobiology, 46, 205-229.

Lovelock, J. E., & Polge, C. (1954). The immobilization of spermatozoa by freezing and thawing and the protective action of glycerol. Byochem J. 58 (4), 618–622.

Oliveira, J. M. A., Maximo, C. M., Oliveira, A. S., & Monteiro, D. C. C. (2018). Implantação da coleção de cultura de microrganismos patogênicos do claretiano em Boa Vista – Roraima. Medicina e Saúde (Rio Claro), 1 (2), 73-82.

Passador, M. M., Pires, G. C. C., Finatti, D., Aparecido, C. C., & Figueiredo, M. B. (2010). Manutenção da viabilidade e patogenicidade de culturas mantidas na micoteca “Mário Barreto Figueiredo”. Biológico, (São Paulo) 72 (1), 51-55.

Saeki, E. K., Farhat, L. P., & Pontes, E. A. (2015). Eficiência dos crioprotetores glicerol e leite desnatado para o congelamento de micro-organismos. Acta Veterinaria Brasilica, 9 (2), 195-198.

Silva, M., & Sá, M. R. (2016). Coleções vivas: as coleções microbiológicas da Fundação Oswaldo Cruz. Museologia & Interdisciplinaridade, 5 (90), 175-187.

Sola, M. C., Oliveira, A. P., Feistel, J. C., & Rezende, C. S. M. (2012). Manutenção de microrganismos: conservação e viabilidade. Enciclopédia Biosfera, (Goiânia), 8 (14), 1398-1418.

Tortora, G. J., Funke, B. R., & Case, C. L. (2017). Microbiologia (12a ed.). Porto Alegre: Artmed.

Wolfe, J., & Bryant, G. (1999) Freezing, drying, and/or vitrification of membrane- solute-water systems. Cryobiology, 39 (2), 103-129.

Published

16/08/2020

How to Cite

Amorim, C. F., Oliveira, A. C. S. de ., & Costa , E. A. S. . (2020). Bacteriotech construction in a higher education institution for teaching and research purposes. Research, Society and Development, 9(9), e212997101. https://doi.org/10.33448/rsd-v9i9.7101

Issue

Section

Health Sciences