Effect of crude extract of genipap (Genipa americana) on the control of the bovine tick Rhipicephalus (Boophilus) microplus

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.7308

Keywords:

Fruit; Parasite; Acaricidal.

Abstract

Tick is the most important ectoparasite among cattle, being favored by the climatic characteristics of Brazil. This parasite commits herds causing disease, stress, allergic reactions and damage to the animal's hide. Due to the successive uses of synthetic acaricides, ticks have developed resistance to these chemicals, leading to research with natural products with the same acaricidal effect. The present study aimed to analyze the effect of crude extract of genipap on the control of R. microplus. The methodology used for the acaricidal efficiency was the immersion test of engorged tick females, in concentrations of 50, 75, 100, 150 and 200 mg mL-1, diluted to a proportion of ethanol and water (70:30), for the positive control used Fipronil and the negative control used only the diluent. The effect on acetylcholinesterase inhibition was evaluated using the in vitro Ellman microplate test. It was observed that the efficiency of the product in all treatments (50 to 200 mg mL-1) had an acaricidal effect against R. microplus, with averages ranging from 64.04 to 99.22%, respectively. With the crude extract it was not possible to observe the inhibition of acetylcholinesterase (5.18%), however, fraction 2 of the extract partition showed an inhibition of 67.4%, demonstrating to have substances capable of inhibiting the enzyme. The crude extract of genipap has shown to be efficient in the control of the bovine tick and must be studied to be part of a strategic ecological control.

References

Ahmed, T., & Gilani, A. H. (2009). Inhibitory effect of curcuminoids on acetylcholinesterase activity and attenuation of scopolamine-induced amnesia may explain medicinal use of turmeric in Alzheimer's disease. Pharmacology Biochemistry and Behavior, 91 (4), 554–559. https://doi.org/10.1016/j.pbb.2008.09.010

Barbosa, C. S., Borges, L. M. F., Nicácio, J., Alves, R. D., Miguita, C. H., Violante, I. M. P., Hamerski, L., Garcez, W. S., & Garcez, F. R. (2013). In vitro activities of plant extracts from the Brazilian Cerrado and Pantanal against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Experimental and Applied Acarology, 60, 421–430. http://dx.doi.org/10.1007/s10493-013-9656-z

Bennett, G. F. (1974). Oviposition of Boophilus microplus (Canestrini) (Acarida Ixodidae). I. Influence of tick size on egg production. Acarologia, 16, 52-61.

Bittencourt, V. R. E. P., et al. (1999). Mecanismo de infecção do fungo Metarhizium anisopliae no carrapato Boophilus microplus em condições experimentais. Ciência Rural, 29 (2), 351-354. https://doi.org/10.1590/S0103-84781999000200028

Clemente, M. A., Gomes, F. T., Scotton, A. C. B. S., Goldner, M. S., Reis, E. S., & Almeida, M. N. (2007). Avaliação do potencial de plantas medicinais no controle de Rhipicephalus microplus (Acari: Ixodidae). Revista Brasileira de Biociências, 5 (2), 516-518.

Domingues, L. F., Giglioti, R., Feitosa, K. A., Fantatto, R. R., Rabelo, M. D., Oliveira, M. C., Oliveira, G. P., Bechara, G. H., & Chagas, A. C. (2013). In vitro activity of pineapple extracts (Ananascomosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Experimental Parasitology, 134 (3), 400–404. https://doi.org/10.1016/j.exppara.2013.03.021

Drummond, R. O., et al. (1973). Boophilus annulatus and Boophilus microplus: Laboratory test of insecticides. Journal Economical Entomology, 66 (1), p.130-133. https://doi.org/10.1093/jee/66.1.130

Ellman, G. L., Courtney, K. D., Andres, V. Jr., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7 (2), 88–95. https://doi.org/10.1016/0006-2952(61)90145-9

Figueiredo, R. W., Maia, G. A., Holanda, L. F. F., & Monteiro, J. C. (1986). Características físicas e químicas do jenipapo. Pesquisa Agropecuária Brasileira, 21 (4), 421-428.

Ghosh, S., Tiwari, S. S., Srivastava, S., Sharma, A. K., Kumar, S., Raya, D. D., & Rawat, A. K. S. (2013). Acaricidal properties of Ricinus communis leaf extracts against organophosphate and pyrethroids resistant Rhipicephalus (Boophilus) microplus. Veterinary Parasitology, 192, 259-267. http://dx.doi.org/10.1016/j.vetpar.2012.09.031

Gonzales, J. C. (2003). O controle do carrapato do boi. 3ª ed. Passo Fundo: Universidade de Passo Fundo, 128.

Heimerdinger, A. (2005). Extrato alcoólico de capim-cidreira (Cymbopogon citratus) no controle do carrapato (Rhipicephalus microplus) de bovinos leiteiros. 78. Dissertação (Mestrado - Área de Produção Animal/Bovinocultura de Leite) - Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria.

Jonsson, N. N., et al. (2001). An estimate of the economic effects of cattle tick (Boophilus microplus) infestation on Queensland dairy farms. Australian Veterinary Journal, 79 (12), 826-831. https://doi.org/10.1111/j.1751-0813.2001.tb10929.x

Lima, A. S., Sousa Filho, J. G. N., Pereira, S. G., Guillon, G. M. S. P., Santos, L. S., & Costa Júnior, L.M. (2014). Acaricide activity of different extracts from Piper tuberculatum fruits against Rhipicephalus microplus. Parasitology Research, 113 (1), 107–112. https://doi.org/10.1590/1809-4392201700053

Omena, C. M. B., et al. (2012). Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits: Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits. Food Reseearch International, 49, 334-344. https://doi.org/10.1016/j.foodres.2012.07.010

Ono, M., Ishimatsu, N., Masuoka, C., Yoshimitsu, H., Tsuchihashi, R., Okama, M., et al. (2007). Three new monoterpenoids from the fruit of Genipa americana. Chemical and Pharmaceutical Bulletin, 55 (4), 632-634. https://doi.org/10.1248/cpb.55.632

Pereira A. S., Shitsuka, D. M., Parreira, F. J. & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria: UAB/NTE/UFSM. Recuperado de https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Pereira, S. G., Araújo, S. A. de, Guilhon, G. M. S. P., Santos, L.S. & Costa Junior, L.M. (2017). In vitro acaricidal activity of Crescentia cujete L. fruit pulp against Rhipicephalus microplus. Parasitology Research, 116 (5), 1487–1493. https://doi.org/10.1007/s00436-017-5425-y

Roel, A. R. (2001). Utilização de plantas com propriedades inseticidas: uma contribuição para o desenvolvimento rural sustentável. Revista Internacional de Desenvolvimento Local, 1 (2), 43-50.

Rodrigues, L. M. A., Bispo, J. L. P., Silva, A. G. da, Gualberto, S. A., Silva, L. F. N., Tavares, I. M. de C., & Oliveira, C. P. de. (2020). Chemical prospection and biological potential of tropical fruit waste extracts. Research, Society and Development, 9 (8), e833986476. https://doi.org/10.33448/rsd-v9i8.6476)

Roulston, W. J., Schnitzerling, H. J., Schuntner, C. A., & Wilson, J. T. (1968). Acetylcholinesterase insensitivity in the Biarra strain of the cattle tick Boophilus microplus, as a cause of resistance to organophosphorus and carbamate acaricides. Australian Journal of Biological Sciences, 21, p. 759–767. https://doi.org/10.1071/BI9680759

Santos, L. B., Souza, J. K., Papassoni, B., Borges, D. G. L., Junior, G. A. D., Souza, J. M. E., Carollo, C. A., & Borges, F. A. (2013). Efficacy of extracts from plants of the Brazilian Pantanal against Rhipicephalus (Boophilus) microplus. Revista Brasileira de Parasitologia Veterinária, 22 (4), 532-538. https://doi.org/10.1590/S1984-29612013000400013

Sousa, L. A. D., Sara, S. F., Pires Júnior, H. B., Ferri, P. H., & Borges, L. M. F. (2008). Avaliação da eficácia de extratos oleosos de frutos verdes e maduros de cinamomo (Melia azedarach) sobre Rhipicephalus (Boophilus) microplus (Acari: ixodidae). Revista Brasileira de Parasitologia Veterinária, 17 (1), 36-40. https://doi.org/10.1590/S1984-29612008000100008

Vasconcelos, V. O., Martins, M. A. D., Oliveira, N. J. F., & Duarte, E. R. (2014). Effect of ethanolic extract of Capsicum frutescens L. on adult female of Rhipicephalus microplus (Ixodidae). Parasitology Research, 113 (4), 1389–1394. https://doi.org/10.1007/s00436-014-3779-y

Vinutha, B., Prashanth, D., Salma, K., Sreeja, S. L., Pratiti, D., Padmaja, R., Radhika, S., Amita, A., Venkateshwarlu, K., & Deepak, M. (2007). Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. Journal of Ethnopharmacology, 109, 359–363. https://doi.org/10.1016/j.jep.2006.06.014

Published

27/08/2020

How to Cite

Bispo, J. L. P., Almeida, E. C. de, & Nunes, D. M. (2020). Effect of crude extract of genipap (Genipa americana) on the control of the bovine tick Rhipicephalus (Boophilus) microplus . Research, Society and Development, 9(9), e481997308. https://doi.org/10.33448/rsd-v9i9.7308

Issue

Section

Agrarian and Biological Sciences