In vitro antimicrobial activity of vegetable extracts on isolated fish thaw
DOI:
https://doi.org/10.33448/rsd-v9i9.7406Keywords:
Vegetable extracts; Origanum vulgare; Curcuma longa; Zingiber officinale; Phenolic compounds.Abstract
This study aimed to determine the yield, phytochemical prospecting, the content of total phenolic compounds and the antimicrobial activity promoted by the plant extracts from the aerial parts Origanum vulgare and the rhizome of Curcuma longa and Zingiber officinale, 70% hydroethanolic obtained in the Central Market of São Luís - MA, Brazil. Extracts were produced from the maceration process in June 2018. The percentage yield was determined from the raw material, the phytochemical screening of the extracts was performed using qualitative colorimetric and precipitation methods. The total phenolic content, was determined using the colorimetric method using the Folin-Ciocalteau reagent. To evaluate the antimicrobial activity, isolates obtained from the thaw of the fish were used. The yield was 20.03%, 26.32% and 2.8%, and for a total phenolic content of 138.79; 250.95 and 100.12 mg EAT 100 g-1, respectively for O. vulgare, C. longa and Z. officinale. The phytochemical screening of the extracts, showed the presence of positive for phenols, alkaloids, triterpenes, flavonones, flavonols, catechins, flavonoids and steroids. The antibacterial inhibition activity showed good results, expressed in minimum inhibition concentration MIC for the three plant extracts evaluated, especially for C. longa, which showed high sensitivity to bacterial isolates. The results obtained in this study, show the extracts of O. vulgare, C. longa and Z. officinale as possible agents in the extraction of total phenolic compounds and as antibacterial and can be used in the food, pharmaceutical, biotechnology and agricultural industries.
References
Andrade, M.A., Cardoso, M.D.G., Batista, L.R., Mallet, A.C.T., & Machado, S.M.F. (2012). Essential oils of Cinnamomum zeylanicum, Cymbopogon nardus and Zingiber officinale: Composition, antioxidant and antibacterial activities. Revista Ciencia Agronômica. (43), 399–408.
Angelo, P. M., & Jorge, N. (2007). Compostos fenólicos em alimentos – Uma breve revisão. Revista do Instituto Adolfo Lutz, 66, 1,1-9.
Anhalt, J. P., & Washington II, J. A. (1985). Preparation and storage of antimicrobial solutions. In: Lennette, E. H., Balows, A., Hausler Jr., W. J. et al. (Ed.) Manual of clinical microbiology. Washington: American Society for Microbiology, 1019-1020.
Atarés. L. & Chiralt, A. (2016). Essential oils as additives in biodegradable films and coatings for active food packaging. Trends in food science & technology. (48). 51-62.
Burque, R. K., Francesconi, L. P., Victorino, A. T., Mascarenhas, M. A., & Ceresér, K. M. . (2015). Determinação de compostos fenólicos e avaliação da atividade antioxidante de Lafoensia pacari (Lythraceae). Revista Eletrônica de Farmácia, v. 12, n. 1. P. 1-10.
CLSI. (2015). & M02-A12: Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - Twelfth Edition.
Dutra, T. V., Castro, J. C., Menezes, J. L., Ramos, T. R., Do Prado, I. N., Júnior, M. M., & De Abreu Filho, B.A. (2019). Bioactivity of oregano (Origanum vulgare) essential oil against Alicyclobacillus spp. Industrial Crops and Products. 129:345-349.
Gebreyes, W. A., Wittum, T.,& Habing, G. et al. Spread of Antibiotic Resistance in Food Animal Production Systems. In: Dodd, C., Aldsworth, T., Stein, R.A. et al. (Ed.). Foodborne Diseases (Third edition). Cambridge: Academic Press, (2017).105-130.
Grégio, A. M. T., Fortes, E. S. M., Rosa, E. A. R., Simeoni, R. B., & Rosa, R. T. (2006). Ação antimicrobiana do Zingiber officinale frente à microbiota bucal. Estudos de Biologia. 28:61.
Gupta, A., Mahajan, S., & Sharma, R. (2015). Evaluation of antimicrobial activity of Curcuma longa rhizome extract against Staphylococcus aureus. Biotechnology reports. (6).51-55.
Matos, F. D. A. (1997). Introdução à fitoquímica experimental. edições UFC.
Menezes Filho, A. C. P. de., & Castro, C .F. de S. (2019a). Identificação das classes metabólicas secundárias em extratos etanólicos foliares de Byrsonima verbascifolia, Cardiopetalum calophyllum, Curatella americana e Qualea grandiflora. Colloquium Agrariae. 15 (4), 39-50.
Menezes Filho, A. C. P. de, Oliveira Filho, J. G., & Christofoli, M.,Castro, C.F. de S. (2019b). Atividade antioxidante e compostos bioativos em espécies de um fragmento de Cerrado goiano tipo cerradão. Colloquium Agrariae, 15, 1, 1-8.
Menezes Filho, A. C. P. de, & Castro, C .F. de S. (2019c). Avaliação de diferentes solventes na extração de compostos fenólicos totais em extratos farináceos do fruto de Hymenaea stigonocarpa L. Brazilian Journal of Food Research, Campo Mourão, 10, 2, 158-169.
Menezes Filho, A. C. P. de, & Castro, C. F. de S. (2020). Identificação das classes fitoquímicas de metabólicos secundários em extratos etanólicos foliares de espécies do cerrado Goiano/GO, Brasil. Revista eixo. Brasília-DF, 9, 2, 41-52.
Menezes Filho, A. C. P. de, Santos, M. C., & Castro, C. F. de S. (2020). Estudo fitoquímico, bioativo, fotoprotetor e físico-químico do extrato floral de algodãozinho do Cerrado [Cochlospermum regiumSchrank. (Pilg.)]–Bixaceae. Revista Arquivos Científicos (IMMES). Macapá, AP, 3, 2.-1-13.
Miranda, C. A. S. F., Das Graças Cardoso, M., Batista, L.R., Rodrigues, L.M.A., & Da Silva Figueiredo, A.C. (2016). Óleos essenciais de folhas de diversas espécies: propriedades antioxidantes e antibacterianas no crescimento espécies patogênicas. Revista Ciência Agronômica. (47). 213-220.
Mishra, R., Gupta, A. K., Kumar, A., Lal, R. K., Saikia, D., & Chanotiya, C.S. (2018). Genetic diversity, essential oil composition, and in vitro antioxidant and antimicrobial activity of Curcuma longa L. germplasm collections. Journal of Applied Research on Medicinal and Aromatic Plants. (10). 75-84.
Oliveira, A. B. A. D., Paula, C. M. D. D., Capalonga, R., Cardoso, M.R.D.I., & Tondo, E.C. (2010). Doenças transmitidas por alimentos, principais agentes etiológicos e aspectos gerais: uma revisão. Revista HCPA. (30).279-285.
Oliveira, T. F. V .D. (2017).Características químicas e microbiológicas do açafrão-da-terra (Curcuma longa). Apucarana: Universidade Tecnológica Federal do Paraná.
Porto, L. L., & Rosa, L. R. V. D. (2018). Avaliação do potencial antimicrobiano de óleos essenciais de coentro (Coriandrum sativum L.) e orégano (Origanum vulgare L.). Ponta Grossa: Universidade Tecnológica Federal do Paraná.
Sankar, R., Karthik, A., Prabu, A., Karthik, S., Shivashangari, K.S., & Ravikumar, V. (2013). Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. Colloids and Surfaces B: Biointerfaces. 108:80-84.
Sarikurkcu. C., Zengin, G., Oskay, M., Uysal, S., Ceylan, R., & Aktumsek, A.(2015). Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Industrial Crops and Products. 70:178-184.
Sasidharan, I., & Menon, A. N. (2010). Comparative chemical composition and antimicrobial activity fresh & dry ginger oils (Zingiber officinale Roscoe). International Journal of Current Pharmaceutical Research. 2, 40-43.
Schirato, G. V., Monteiro, F. M. F., Silva, F. de. O., Lima Filho, J. de. L., Leão, A. M. dos. A.C., & Porto, A.L.F. (2006). The polysaccharide from Anacardium occidentaleL. in the inflammatory phase of the cutaneous wound healing. Ciência Rural, 36, 1, 149-154.
Silva, F.T. (2018). Ação do óleo essencial de gengibre (Zingiber officinale) encapsulado em fibras ultrafinas de proteína isolada de soja, poli (óxido de etileno) e zeína no controle antimicrobiano in situ. Pelotas: Universidade Federal de Pelotas.
Singh, G., Kapoor, I. P.S ., Singh, P., Heluani, C.S., Lampasona, M. P., & Catalan, C. A. N. (2008). Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food and Chemical Toxicology. 46:3295.
Singh, S., Sankar, B., Rajesh, S., Sahoo, K., Subudhi, E., & Nayak, S. (2011). Chemical composition of turmeric oil (Curcuma longa L. cv. Roma) and its antimicrobial activity against eye infecting pathogens. Journal of Essential Oil Research. 23,11-18.
Soković, M., Glamočlija, J., Marin, P. D., Brkić, D., & Van Griensven, L.J. (2010).Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules. 15:7532-7546.
Solórzano-Santos, F., Miranda-Novales, & M .G .(2012).Essential oils from aromatic herbs as antimicrobial agents. Current Opinion in Biotechnology. 23, 136–141.
Souza, C .N., De Almeida, A. C., Xavier, M. T. R., Costa, J. P.R ., Da Silva, L .M .V., Martins, E.R. (2017).Atividade antimicrobiana de plantas medicinais do cerrado mineiro frente abacterias isoladas de ovinos com mastite. Unimontes Científica. 19, 51-61.
Stefanakis, M. K., Touloupakis, E., Anastasopoulos, E., Ghanotakis, D., Katerinopoulos, H.E., & Makridis, P. (2013). Antibacterial activity of essential oils from plants of the genus Origanum. Food control. 34, 539-546.
Tajkarimi, M. M., Ibrahim Salam, A., & Cliver, D.O. (2010). Antimicrobial herb and spice compounds in food. Food control.21, 1199-1218.
Teixeira-Loyola, A.B.A., Siqueira, F.C., Paiva, L.F., & Schreiber, A.Z. (2014).Análise Microbiológica de especiarias comercializadas em Pouso Alegre, Minas Gerais. Revista Eletrônica Acervo Saúde. 6, 515-529.
Teles, A. M., Mouchreck, A. N., & Everton, G. O., Abreu-Silva, A. L., Calabrese, K. S., Almeida-Souza, F. (July 30th 2019a). Comparative Analysis of the Chemical Composition, Antimicrobial and Antioxidant Activity of Essential Oils of Spices Used in the Food Industry in Brazil, Essential Oils - Oils of Nature, Hany A. El-Shemy, IntechOpen.
Teles, A. M., Santos, B. A., Ferreira,C. G., Mouchreck, A. N., Calabrese, K. S., Abreu-Silva, A.L., & Almeida-Souza, F. (December 6th 2019b). Ginger (Zingiber officinale) Antimicrobial Potential: A Review, Ginger Cultivation and Its Antimicrobial and Pharmacological Potentials, Haiping Wang, IntechOpen.
.
Teles, A. M., Rosa, T. D., Mouchrek, A. N., Abreu-Silva, A. L., Calabrese, K. D., & Almeida-Souza, F. (2019c). Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity. Evidence-based Complementary and Alternative Medicine :eCAM.
Yap, P. S. X., Yiap, B. C., & Ping, H. C. (2004) .Essential oils, a new horizon in combating bacterial antibiotic resistance. The Open Microbiology Journal. 2004:8:6-14.
Veber, J., Petrini, L. A., Andrade, L. B., & Siviero, J. (2015). Determinação dos compostos fenólicos e da capacidade antioxidante de extratos aquosos e etanólicos de Jambolão (Syzygium cumini L.) . Revista Brasileira de Plantas Medicinais. Campinas, 17, 2, 267-273.
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Copyright (c) 2020 Isadora do Nascimento Oliveira; Amanda Mara Teles; Geisa Correia Santos; Catarina da Silva Saboia; Bianca Araújo Santos; Danilo Torres Cardoso; Adenilde Nascimento Mouchrek
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