Modulating effect of Poincianella bracteosa (Tul.) L.P. Queiroz bark (Leguminosae) on DNA damage induced by doxorubicin on the somatic cells of Drosophila melanogaster wings

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.7833

Keywords:

Antimutagenic; Catingueira; Medicinal plant; SMART.

Abstract

The aim of this study was to assess the genotoxic and antigenotoxic effects of Poincianella bracteosa bark aqueous extract on DNA damage induced by doxorubicin (DXR) a chemotherapeutic agent using SMART (Somatic Mutation and Recombination Test). The analysis was performed using the somatic mutation and recombination test in Drosophila melanogaster. Larvae from the standard and high-bioactivity crosses were chronically treated with four concentrations of P. bracteosa bark tea, alone and in association with DXR. The results revealed no mutagenic effect of bark extract for any of the concentrations tested. A modulating effect of aqueous extract in reducing the genotoxic action of DXR was observed for all concentrations tested in descendants of both crosses, but inhibition was more effective in those from the high-bioactive cross. The modulating effect observed may be associated with the presence of tannins and reducing sugars, as observed in phytochemical studies, since they are capable of capturing and stabilizing free radicals. Given the widespread use of P. bracteosa bark in folk medicine, further studies to elucidate the mechanism of action of these cellular compounds and with other experimental models would be useful to confirm that P. bracteosa extract is beneficial to human health.

References

Abraham, S. K. (1994). Antigenotoxicity of coffee in the Drosophila assay for somatic mutation and recombination. Mutagenesis, 9(4), 383-386. https://doi.org/10.1093/mutage/9.4.383

Barbosa, W. L. R, Quignard, E., Tavares, I. C. C., Pinto, L. N., Oliveira, F. Q. & Oliveira, R. M. (2004). Manual para Análise fitoquímica e Cromatografia de Extratos Vegetais (Edição Revisada). Revista Científica da UFPA 4. Recuperado em: http://www.ufpa.br/rcientifica.

Bosire, C. M., Deyou, T., Kabaru, J.M., Kimata, D.M. & Yenesew, A. (2014). Larvicidal activities of the stem bark extract and rotenoids of Millettia usaramensis subspecies usaramensis on Aedes aegypti L. (Diptera: Culicidae). Journal of Asia-Pacific Entomology, 17, 531-535. https://doi.org/10.1016/j.aspen.2014.05.003

Castro, A. S. F. & Cavalcante, A. M. B. (2011). Flores da Caatinga. Campina Grande: Instituto Nacional do Semiárido, 30-31.

Chaves, E. M. F. & Barros, R. F. M. (2012). Diversidade e uso de recursos medicinais do carrasco na APA da Serra da Ibiapaba, Piauí, Nordeste do Brasil. Revista Brasileira de Plantas Medicinais, 14(3), 476-486. http://dx.doi.org/10.1590/S1516-05722012000300009

Cruz, R. C. D., Carvalho, K. S., Silva, S. L. C., Gualberto, S. A. & Macedo, G. E. L. (2015). Bioatividade da raíz de Poincianella bracteosa (Tul.) L. P. Queiroz (Fabaceae) sobre larvas do Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae). Revista Brasileira de Biociências, 13 (4), 259-264. Recuperado em:http://www.ufrgs.br/seerbio/ojs/index.php/rbb/article/view/3399

Felício, L. P., Silva, E. M., Ribeiro, V., Miranda, C. T., Vieira, I. L. B. F., Passos, D. C. S., Ferreira, A. K. S., Vale, C. R., Lima, D. C. S., Carvalho, W. B. & Nunes, W. B. (2011). Mutagenic potential and modulatory effects of the medicinal plant Luehea divaricata (Malvaceae) in somatic cells of Drosophila melanogaster: SMART/wing. Genetics and Molecular Researh, 10(1), 16-24. https://doi.org/10.4238/vol10-1gmr982

Fernandes, F. H., Guterres, Z. R., Garcez, W.S., Lopes, S.M., Corsino, J. & Garcez, F. G. (2014). Assessment of the (anti)genotoxicity of brown propolis extracts from Brazilian Cerrado biome in a Drosophila melanogaster model. Food Research International, 62, 20-26. https://doi.org/10.1016/j.foodres.2014.02.029

Fernandes, L. M., Garcez, W. S., Mantovani, M. S., Figueiredo, P. O., Fernandes, C. A., Garcez, F. R. & Guterres, Z. R. (2013). Assessment of the in vitro and in vivo genotoxicity of extracts and indole monoterpene alkaloid from the roots of Galianthe thalictroides (Rubiaceae). Food and Chemical Toxicology, 59, 405-411. https://doi.org/10.1016/j.fct.2013.05.052

Franchi, L. P., Guimarães, N. N., Andrade, L. R., Andrade, H. H. R., Lehmann, M., Dihl, R. R. & Cunha, K. S. (2013). Antimutagenic and antirecombinagenic activities of noni fruit juice in somatic cells of Drosophila melanogaster. Anais da Academia Brasileira de Ciências, 85(2), 585-594. https://doi.org/10.1590/S0001-37652013000200008

Frei, H. & Würgler, F. E. (1995). Optimal experimental design and simple size for the statistical evaluation of data from somatic mutation and recombination tests (SMART) in Drosophila. Mutatation Research, 334(2), 247-258. https://doi.org/10.1016/0165-1161(95)90018-7

Frei, H. & Würgler, F. E. (1988). Statistical methods to decide whether mutagenicity test data from Drosophila assays indicate a positive, negative, or inconclusive result. Mutation Research, 203(4), 297-308. https://doi.org/10.1016/0165-1161(88)90019-2

Graf, U., Abraham, S. K., Rincón, J. G. & Würgler, F. E. (1998). Antigenotoxicity studies in Drosophila melanogaster. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 402(1-2), 203-209. https://doi.org/10.1016/S0027-5107(97)00298-4

Graf, U. & Singer, D. (1989). Somatic mutation and recombination test in Drosophila melanogaster (wing-spot test): Effects of extracts of airborne particulate matter from fire-exposed and non-fire-exposed building ventilation filters. Chemosphere, 19(7), 1094-1097. https://doi.org/10.1016/0045-6535(89)90533-X

Graf, U. & Van Schaik, N. (1992). Improved high bioactivation cross for the wing somatic mutation and recombination test of Drosophila melanogaster. Mutation Research, 271(1), 59-67. https://doi.org/10.1016/0165-1161(92)90032-H

Graf, U., Wügler, F. E., Katz, A. J., Frei, H., Juon, H., Hall, C. B. & Kale, P. G. (1984). Somatic mutation and recombination test in Drosophila melanogaster. Environmental Mutagenesis, 6(2), 153-188. https://doi.org/10.1002/em.2860060206

Guterres, Z. R., Silva, A. F. G., Garcez, W. S., Garcez, F. R., Fernandes, C. A. & Garcez, F. R. (2013). Mutagenicity and recombinagenicity of Ocotea acutifolia (Lauraceae) aporphinoid alkaloids. Mutation Research, 757(1), 91-96. https://doi.org/10.1016/j.mrgentox.2013.07.004

Jacociunas, L. V., Dihl, R. R., Lehmann, M., Ferraz, A. B. F., Richter, M. F., Silva, J. & Andrade, H. H. R. (2014). Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster. Genetics and Molecular Biology, 37(1), 93-104. https://doi.org/10.1590/S1415-47572014000100015

Kiraithe, M. N., Nguta, J. M., Mbaria, J. M. & Kiama, S. G. (2016). Evaluation of the use of Ocimum suave Willd. (Lamiaceae), Plectranthus barbatus Andrews (Lamiaceae) and Zanthoxylum chalybeum Engl. (Rutaceae) as antimalarial remedies in Kenyan folk medicine. Journal of Ethnopharmacology, 178, 266-271. https://doi.org/10.1016/j.jep.2015.12.013

Lewis, G. P. (2015). Poincianella in Lista de Espécies da Flora do Brasil. (Jardim Botânico do Rio de Janeiro). Recuperado de: http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB109774

Maia-Silva, C., Silva, C. I., Hrncir, M., Queiroz, R. T. & Imperatriz-Fonseca, V. L. (2012). Guia de plantas: visitadas por abelhas na Caatinga (1ª ed.). Fortaleza: Fundação Brasil Cidadão. Recuperado de: https://www.mma.gov.br/estruturas/203/_arquivos/livro_203.pdf

Monteiro, J. M., Alburquerque, U. P. & Araújo, E. L. (2005). Taninos: uma abordagem da química à ecologia. Química Nova, 28(5), 892-896. https://doi.org/10.1590/S0100-40422005000500029

Monteiro, J. M., Souza, J. S. N., Neto, E. M. F. L., Scopel, K. & Trindade, E. F. (2014). Does total tannin content explain the use value of spontaneous medicinal plants from the Brazilian semi-arid region? Revista Brasileira de Farmacognosia, 24(2), 116-123. https://doi.org/10.1016/j.bjp.2014.02.001

Nguta, J. M., Opong, R. A., Nyarko, A. K., Manu, D. Y., Addo, P. G. A., Otchere, I. & Twum, K. (2016). Antimycobacterial and cytotoxic activity of selected medicinal plant extracts. Journal of Ethnopharmacology, 182, 10-15. https://doi.org/10.1016/j.jep.2016.02.010

Orsolin, P. C., Oliveira, R. G. S. & Nepomuceno, J. C. (2016). Modulating effect of simvastatin on the DNA damage induced by doxorubicin in somatic cells of Drosophila melanogaster. Food and Chemical Toxicology, 90, 10-17. https://doi.org/10.1016/j.fct.2016.01.022

Ouedraogo, M., Baudoux, T., Stévigny, C., Nortier, J., Colet, J. M., Efferth, T., Qu, F., Zhou, J., Chan, K., Shaw, D., Pelkonen, O. & Duez, P. (2012). Review of current and “omics” methods for assessing the toxicity (genotoxicity, teratogenicity and nephrotoxicity) of herbal medicines and mushrooms. Journal of Ethnopharmacology, 140(3), 492-512. https://doi.org/10.1016/j.jep.2012.01.059

Pandey, U. B. & Nichols, C. D. (2011). Human Disease Models in Drosophila melanogaster and the Role of the Fly in Therapeutic Drug Discovery. Pharmacological Reviews, 63(2), 411-436. https://doi.org/10.1124/pr.110.003293

Patenkovic, A., Stamenkovic-Radak, M., Nikolic, D., Markovic, T. & Andelkovic, M. (2013). Synergistic effect of Gentiana lutea L. methyl methanesulfonate genotoxicity in the Drosophila wing spot test. Journal of Ethnophrmacology, 146(2), 632-636. https://doi.org/10.1016/j.jep.2013.01.027

Pereira, D. G., Antunes, L. M. G., Graf, U. & Spanó, M. A. (2008). Protection by Panax ginseng C.A. Meyer against the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster. Genetic and Molecular Biology, 31(4), 947-955. https://doi.org/10.1590/S1415-47572008000500024

Ping, K. Y., Darah, I., Yusuf, U. K., Yeng, C. & Sasidharan, S. (2012). Genotoxicity of Euphorbia hirta: An Allium cepa Assay. Molecules, 17, 7782-7791. https://doi.org/10.3390/molecules17077782

Rezende, A. A. A., Silva, M. L. A., Tavares, D. C., Cunha, W. R., Rezende, K. C. S., Bastos, J. K., Lehmann, M., de Andrade, H. H. R., Guterres, Z. R., Silva, L. P. & Spanó, M. A. (2011). The effect of the dibenzylbutyrolactolic lignan (-)-cubebin on doxorubicin mutagenicity and recombinogenicity in wing somatic cells of Drosophila melanogaster. Food and Chemical Toxicology, 49(6), 1235-1241. https://doi.org/10.1016/j.fct.2011.03.001

Santos, A. A., Deoti, J. R., Müller, G., Dário, M. G., Stambuk, B. U. & Junior, S. L. A. (2017). Dosagem de açúcares redutores com o reativo DNS em microplaca. Brazilian Journal of Food Technology, 20. https://doi.org/10.1590/1981-6723.11315

Saraiva, M. E., Ulisses, A. V., Ribeiro, D. A., Oliveira, L. G., Macêdo, D. G., Sousa, F. F., Menezes, I. R., Sampaio, E. V., Souza, M. M. (2015). Plant species as a therapeutic resource in areas of the savanna in the state of Pernambuco, Northeast Brazil. Journal of Ethnopharmacology, 171, 141-153. https://doi.org/10.1016/j.jep.2015.05.034

Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M. & Latha, L. Y. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1). https://doi.org/10.4314/ajtcam.v8i1.60483

Saturnino, R. S., Machado, N. M., Lopes, J. C. & Nepomuceno, J. C. (2018). Assessment of the mutagenic, recombinogenic, and carcinogenic potential of amphotericin B in somatic cells of Drosophila melanogaster. Drug and Chemical Toxicology, 41(1), 9-15. https://doi.org/10.1080/01480545.2016.1188302

Silva, J. D. A., Nascimento, M. G. P., Grazina, L. G., Castro, K. N. C., Mayo, S. J. & Andrade, I. M. (2015). Ethnobotanical survey of medicinal plants used by the community of Sobradinho, Luís Correia, Piauí, Brazil. Journal of Medicinal Plants Research, 9(32), 872-883. https://doi.org/ 10.5897/JMPR2015.5881

Sponchiado, G., Adam, M. L., Silva, C. D., Soley, B. S., Sampayo, C. M., Cabrini, D. A., Correr, C. J., Otuki MF. (2016). Quantitative genotoxicity assays for analysis of medicinal plants: A systematic review. Journal of Ethnopharmacology, 178, 289-296. https://doi.org/ 10.1016 / j.jep.2015.10.026

Vale, C. R., Silva, C. R., Oliveira, C. M. A., Silva, A. L., Carvalho, S. & Chen, L. C. (2013). Assessment of toxic, genotoxic, antigenotoxic, and recombinogenic activities of Hymenaea courbaril (Fabaceae) in Drosophila melanogaster and mice. Genetics and Molecular Research, 12(3), 2712-2724. http://dx.doi.org/10.4238/2013.July.30.9

Vargas, F. S., Almeida, P. D. O., Boleti, A. P. A., Pereira, M. M., Souza, T. P., Vasconcellos, M. C., Nunez, C. V., Pohlit, A. M., Lima, E. S. (2016). Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory. BMC Complementary and Alternative Medicine, 16(83). https://doi.org/10.1186/s12906-016-1061-9

Zafred, R. R. T., Spano, M. A., Martins, G. R., Figueiredo, C. C. M., Ferreira, P. C., Silva, L. P. & Silva, R. M. G. (2016). Pro-oxidant activity and genotoxicity of the Astronium fraxinifolium using wings SMART and Allium cepa Test. Research Journal of Medicinal Plants, 10(4), 276-285. https://doi.org/10.3923/rjmp.2016.276.285

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Published

06/09/2020

How to Cite

Oliveira, R. de S. ., Moura, D. de C. ., Sousa, R. M. S. ., Silva, T. T. da ., Oliveira, M. das D. A., Costa Júnior, J. S. da, Almeida, P. M. de, & Martins, F. A. (2020). Modulating effect of Poincianella bracteosa (Tul.) L.P. Queiroz bark (Leguminosae) on DNA damage induced by doxorubicin on the somatic cells of Drosophila melanogaster wings. Research, Society and Development, 9(9), e745997833. https://doi.org/10.33448/rsd-v9i9.7833

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Agrarian and Biological Sciences