Detection of myenteric plexus neurons in dyslipidemic, smoking, and diabetic rats treated with carqueja

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.8093

Keywords:

Baccharis trimera; Hypercholesterolemia; Hyperglycemia; Myenteric plexus.

Abstract

The plant species Baccharis trimera presents antioxidants that may have neuroprotective effects on the neurons of the myenteric plexus. Thus, the aim of the present study was to investigate possible quantitative alterations in the myenteric plexus neurons and in the glycemic and lipid profile of 25 rats with 90 days old, exposed to smoking, a hypercholesterolemic diet, and with diabetes mellitus induced by streptozotocin during four weeks, and then treated with different doses of carqueja extract for two weeks. The myenteric plexus neurons were stained with basic Giemsa and using the NADPH-diaphorase histochemistry protocol. In the study conditions, there was a significant reduction in the number of total neurons between the groups treated with carqueja and the positive control, stained with the Giemsa. In contrast, there was no significant difference in the number of neurons of the inhibitory subpopulation between the groups treated with carqueja and the negative control, evidenced by the NADPH-diaphorase histochemistry. At the 30mg/kg dose there was a reduction in the cholesterol and triglyceride levels. Based on the results, Baccharis trimera presented no neuroprotective or hypoglycemic effect, although the nitric subpopulation has proven more resistant to the deleterious effects of diabetes, smoking, and the hypercholesterolemic diet.

References

American Diabetes Association. (2019). 9. Abordagens farmacológicas para o tratamento glicêmico: Padrões de Cuidados Médicos no Diabetes — 2019. Cuidados com a diabetes , 42 (Suplemento 1), S90-S102. Retrieved May 21, 2020, from www.diabetes.org/diabetescare

Asmat, U., Abad, K., & Ismail, K. (2016). Diabetes mellitus and oxidative stress—A concise review. Saudi pharmaceutical journal, 24(5), 547-553. https://doi.org/10.1016/j.jsps.2015.03.013

Barbosa, A., & AJ, A. B. (1978). Técnica histológica para gânglios nervosos intramurais em preparados espessos. Retrieved Ago 5, 2019, from https://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=PASCAL7950158800

Bastida, G., & Beltrán, B. (2011). Ulcerative colitis in smokers, non-smokers and ex-smokers. World journal of gastroenterology: WJG, 17(22), 2740. https://doi.org/10.3748/wjg.v17.i22.2740

Belai, A., Cooper, S., & Burnstock, G. (1995). Effect of age on NADPH-diaphorase-containing myenteric neurones of rat ileum and proximal colon. Cell and tissue research, 279(2), 379-383. Retrieved Ago 5, 2019, from https://link.springer.com/article/10.1007/BF00318495

Bernstein, C. N., Eliakim, A., Fedail, S., Fried, M., Gearry, R., Goh, K. L., ... & Ouyang, Q. (2016). World gastroenterology organisation global guidelines inflammatory bowel disease: update August 2015. Journal of clinical gastroenterology, 50(10), 803-818. https://doi.org/ 10.1097/MCG.0000000000000660

Cakir, Y., Yang, Z., Knight, C. A., Pompilius, M., Westbrook, D., Bailey, S. M., ... & Ballinger, S. W. (2007). Effect of alcohol and tobacco smoke on mtDNA damage and atherogenesis. Free Radical Biology and Medicine, 43(9), 1279-1288. https://doi.org/10.1016/j.freeradbiomed.2007.07.015

Camilleri, M., Bharucha, A. E., & Farrugia, G. (2011). Epidemiology, mechanisms, and management of diabetic gastroparesis. Clinical Gastroenterology and Hepatology, 9(1), 5-12. https://doi.org/10.1016/j.cgh.2010.09.022

Carver, T. W., Vora, R. S., & Taneja, A. (2016). Mesenteric ischemia. Critical care clinics, 32(2), 155-171. https://doi.org/10.1016/j.ccc.2015.11.001

Chandrasekharan, B., & Srinivasan, S. (2007). Diabetes and the enteric nervous system. Neurogastroenterology & Motility, 19(12), 951-960. https://doi.org/10.1111/j.1365-2982.2007.01023.x

Cowen, T., Johnson, R. J. R., Soubeyre, V., & Santer, R. M. (2000). Restricted diet rescues rat enteric motor neurones from age related cell death. Gut, 47(5), 653-660. Retrieved Ago 5, 2019, from https://gut.bmj.com/content/gutjnl/47/5/653.full.pdf

Cronenwett, J. L., & Johnston, K. W. (2014). Rutherford’s vascular surgery, vol. 2.

Davison, G. W., George, L., Jackson, S. K., Young, I. S., Davies, B., Bailey, D. M., ... & Ashton, T. (2002). Exercise, free radicals, and lipid peroxidation in type 1 diabetes mellitus. Free Radical Biology and Medicine, 33(11), 1543-1551. https://doi.org/10.1016/S0891-5849(02)01090-0

Feldman, E. L., Nave, K. A., Jensen, T. S., & Bennett, D. L. (2017). New horizons in diabetic neuropathy: mechanisms, bioenergetics, and pain. Neuron, 93(6), 1296-1313. https://doi.org/10.1016/j.neuron.2017.02.005

Ferezin, R. I., Vicentino-Vieira, S. L., Góis, M. B., Araújo, E. J. D. A., Melo, G. D. A. N. D., Garcia, J. L., & Sant'Ana, D. D. M. G. (2017). Different inoculum loads of Toxoplasma gondii induce reduction of myenteric neurons of the rat colon. Revista Brasileira de Parasitologia Veterinária, 26(1), 47-53. https://doi.org/10.1590/s1984-29612017003

Fochi G. R. A. Tabagismo: uma revisão. J Psiquiatria 2003; 4(2): 127-135. Retrieved Ago 6, 2019, from http://ri.unir.br/jspui/handle/123456789/1262

Fonseca, K. P., & Abi Rached, C. D. (2019). Complicações do diabetes mellitus. International Journal of Health Management Review, 5(1). Retrieved Ago 6, 2019, from https://www.ijhmreview.org/ijhmreview/article/view/149.

Furlan, M. M. D., Molinari, S. L., & Miranda Neto, M. H. D. (2002). Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats. Arquivos de neuro-psiquiatria, 60(3A), 576-581. http://dx.doi.org/10.1590/S0004-282X2002000400012

Furlan, M. M. D. P., Molinari, S. L., & Neto, M. H. M. (2004). Resposta dos neurônios mioentéricos do duodeno de ratos ao diabetes de curto prazo. Arquivos de Ciências da Saúde da UNIPAR, 8(2). Retrieved Ago 6, 2019, from https://revistas.unipar.br/index.php/saude/article/viewFile/222/195.

Furman, B. L. (2015). Streptozotocin‐induced diabetic models in mice and rats. Current protocols in pharmacology, 70(1), 5-47. https://doi.org/10.1002/0471141755.ph0547s70

Furness, J. B. (2012). The enteric nervous system and neurogastroenterology. Nature reviews Gastroenterology & hepatology, 9(5), 286. https://doi.org/10.1038/nrgastro.2012.32

Furness, J. B. (2006). Novel gut afferents: Intrinsic afferent neurons and intestinofugal neurons. Autonomic Neuroscience, 125(1-2), 81-85. https://doi.org/10.1016/j.autneu.2006.01.007

Gagliardo, K. M., Clebis, N. K., Stabille, S. R., Mari, R. D. B., De Sousa, J. M. A., & De Souza, R. R. (2008). Exercise reduces inhibitory neuroactivity and protects myenteric neurons from age-related neurodegeneration. Autonomic Neuroscience, 141(1-2), 31-37. https://doi.org/10.1016/j.autneu.2008.04.009

Giri, B., Dey, S., Das, T., Sarkar, M., Banerjee, J., & Dash, S. K. (2018). Chronic hyperglycemia mediated physiological alteration and metabolic distortion leads to organ dysfunction, infection, cancer progression and other pathophysiological consequences: an update on glucose toxicity. Biomedicine & Pharmacotherapy, 107, 306-328. https://doi.org/10.1016/j.biopha.2018.07.157

Gois, M. B., Hermes-Uliana, C., Paltanin, A., Pontes, W., Araújo, E. J. A., & Miranda, M. H. N. (2016). Morphoquantitative study of Rattus norvegicus submucosal plexus by different neuronal evidentiation histochemical techniques. Int. j. morphol, 34(4), 1487-1493. Retrieved Ago 6, 2019, from http://www.intjmorphol.com/wp-content/uploads/2017/01/art_50_344.pdf

HERNANDES, L., BAZOTTE, R. B., GAMA, P., & MIRANDA-NETO, M. H. D. (2000). Streptozotocin-induced diabetes duration is important to determine changes in the number and basophily of myenteric neurons. Arquivos de Neuro-Psiquiatria, 58(4), 1035-1039. https://doi.org/10.1590/S0004-282X2000000600010

Irwin, D. A. (1931). The anatomy of Auerbach's plexus. American Journal of Anatomy, 49(1), 141-166. https://doi.org/10.1002/aja.1000490106

Jaldin, R. G., Falcão Filho, H. A., Sequeira, J. L., & Yoshida, W. B. (2006). O processo aterosclerótico em artérias de coelhos submetidos a dieta suplementada com gema de ovo: modelo experimental de baixo custo. Jornal Vascular Brasileiro, 5(4), 247-256. https://doi.org/10.1590/S1677-54492006000400003

Kanamori, K. S., Oderich, G. S., Fatima, J., Sarac, T., Cha, S., Kalra, M., ... & Bower, T. C. (2014). Outcomes of reoperative open or endovascular interventions to treat patients with failing open mesenteric reconstructions for mesenteric ischemia. Journal of vascular surgery, 60(6), 1612-1619. https://doi.org/10.1016/j.jvs.2014.08.093

Kolkman, J. J., & Geelkerken, R. H. (2017). Diagnosis and treatment of chronic mesenteric ischemia: An update. Best practice & research Clinical gastroenterology, 31(1), 49-57. https://doi.org/10.1016/j.bpg.2017.01.003

Ksiazek, K., & Wiśniewska, J. (2001). The role of glucose and reactive oxygen species in the development of vascular complications of diabetes mellitus. Przeglad lekarski, 58(10), 915-918. Retrieved Oct 21, 2019, from https://pubmed.ncbi.nlm.nih.gov/11957818/

Lívero, F. A. R., Martins, G. G., Telles, J. E. Q., Beltrame, O. C., Biscaia, S. M. P., Franco, C. R. C., ... & Acco, A. (2016). Hydroethanolic extract of Baccharis trimera ameliorates alcoholic fatty liver disease in mice. Chemico-biological interactions, 260, 22-32. https://doi.org/10.1016/j.cbi.2016.10.003

Miranda-Neto, M. H., Molinari, S. L., Natali, M. R. M., & Sant'Ana, D. D. M. G. (2001). Regional differences in the number and type of myenteric neurons of the ileum of rats: a comparison of techniques of the neuronal evidentiation. Arquivos de neuro-psiquiatria, 59(1), 54-59. https://doi.org/10.1590/S0004-282X2001000100012

Oyenihi, A. B., Ayeleso, A. O., Mukwevho, E., & Masola, B. (2015). Antioxidant strategies in the management of diabetic neuropathy. Biomed Res Int, 2015(515042), 515042. https://dx.doi.org/10.1155/2014/515042

Pádua, B. C., Victor Rossoni Junior, J., Lopes de Brito Magalhaes, C., Brandao Seiberf, J., Morais Araujo, C., Henrique Bianco de Souza, G., ... & Caldeira Costa, D. (2013). Baccharis trimera improves the antioxidant defense system and inhibits iNOS and NADPH oxidase expression in a rat model of inflammation. Current Pharmaceutical Biotechnology, 14(11), 975-984. https://doi.org/10.2174/1389201014666131226151728

Pádua, B. C., Silva, L. D., Júnior, J. V. R., Humberto, J. L., Chaves, M. M., Silva, M. E., ... & Costa, D. C. (2010). Antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats. Journal of Ethnopharmacology, 129(3), 381-386. https://doi.org/10.1016/j.jep.2010.04.018

Pecoraro, F., Rancic, Z., Lachat, M., Mayer, D., Amann-Vesti, B., Pfammatter, T., ... & Veith, F. J. (2013). Chronic mesenteric ischemia: critical review and guidelines for management. Annals of vascular surgery, 27(1), 113-122. https://doi.org/10.1016/j.avsg.2012.05.012

Phillips, R. J., Kieffer, E. J., & Powley, T. L. (2003). Aging of the myenteric plexus: neuronal loss is specific to cholinergic neurons. Autonomic Neuroscience, 106(2), 69-83. https://doi.org/10.1016/S1566-0702(03)00072-9

Qu, Z. D., Thacker, M., Castelucci, P., Bagyanszki, M., Epstein, M. L., & Furness, J. B. (2008). Immunohistochemical analysis of neuron types in the mouse small intestine. Cell and tissue research, 334(2), 147-161. https://doi.org/10.1007/s00441-008-0684-7

Rocha, F. D., Teixeira, V. L., Pereira, R. C., & Kaplan, M. A. C. (2006). Diabetes mellitus e estresse oxidativo: produtos naturais como alvo de novos modelos terapêuticos. Rev Bras Farm, 87(2), 49-54. Retrieved Oct 18, 2019, from http://www.rbfarma.org.br/files/pag_49a54_DIABETES_MELLITUS.pdf

Sant'Ana, D. M., Araújo, E. J., Ramos, D. H., Hermes-Uliana, C., & Natali, M. R. M. (2012). Characterization of the myenteric neuronal population and subpopulation of the duodenum of adult wistar rat fed with hypoproteic chow. Anais da Academia Brasileira de Ciências, 84(3), 799-806. h ttps://doi.org/10.1590/S0001-37652012005000050

Scherer-Singler, U., Vincent, S. R., Kimura, H., & McGeer, E. G. (1983). Demonstration of a unique population of neurons with NADPH-diaphorase histochemistry. Journal of neuroscience methods, 9(3), 229-234. https://doi.org/10.1016/0165-0270(83)90085-7

Serenini, G. F., Beltrami, J. M., Gerônimo, E., Favetta, P. M., Legnani, N. G., Otutumi, L. K., ... & Germano, R. M. (2020). Quantification of the neurons of myenteric plexus of the bat molossus rufus. Pesquisa Veterinária Brasileira, 40(6), 493-500. https://doi.org/10.1590/1678-5150-pvb-6381

Shakeel, M. (2015). Recent advances in understanding the role of oxidative stress in diabetic neuropathy. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 9(4), 373-378. https://doi.org/10.1016/j.dsx.2014.04.029

Silva Porto, G., Pereira, J. N. B., Tiburcio, V. G., Stabille, S. R., de Faria, H. G., de Melo Germano, R., & de Britto Mari, R. (2012). Effect of caloric restriction on myenteric neuroplasticity in the rat duodenum during aging. Autonomic Neuroscience, 168(1-2), 43-47. https://doi.org/10.1016/j.autneu.2012.01.006

Silverio, S. M., Mari, R. D. B., Clebis, N. K., Scoz, J. R., Germano, R. D. M., Major, J. A., ... & Stabille, S. R. (2009). Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats. Pesquisa Veterinária Brasileira, 29(4), 295-302. http://dx.doi.org/10.1590/S0100-736X2009000400004

Souza, M. M. Q., Silva, G. R. D., Cola, I. M., Silva, A. O., Schaedler, M. I., Guarnier, L. P., ... & Auth, P. A. (2020). Baccharis trimera (Less.) DC: An Innovative Cardioprotective Herbal Medicine Against Multiple Risk Factors for Cardiovascular Disease. Journal of Medicinal Food, 23(6), 676-684. https://doi.org/10.1089/jmf.2019.0165

Wester, T., O’Briain, D. S., & Puri, P. (1999). Notable postnatal alterations in the myenteric plexus of normal human bowel. Gut, 44(5), 666-674. Retrieved Oct 11, 2019, from https://gut.bmj.com/content/44/5/666.abstract

Zanoni, J. N., Buttow, N. C., Bazotte, R. B., & Neto, M. M. (2003). Evaluation of the population of NADPH-diaphorase-stained and myosin-V myenteric neurons in the ileum of chronically streptozotocin-diabetic rats treated with ascorbic acid. Autonomic Neuroscience, 104(1), 32-38. https://doi.org/10.1016/S1566-0702(02)00266-7

Zhao, L., Zhang, F., Ding, X., Wu, G., Lam, Y. Y., Wang, X., ... & Yu, L. (2018). Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes. Science, 359(6380), 1151-1156. https://doi.org/10.1126/science.aao5774

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12/09/2020

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FAVETTA, P. M.; LÍVERO , F. A. dos R. .; SILVA , G. R. da .; SERENINI , G. de F. .; GERONIMO, E.; TRINDADE , W. A. .; MIZUGUCHI, N. N. .; SOUZA JÚNYOR , F. de .; OTUTUMI , L. K. .; SOARES, A. A. .; GERMANO , R. de M. . Detection of myenteric plexus neurons in dyslipidemic, smoking, and diabetic rats treated with carqueja. Research, Society and Development, [S. l.], v. 9, n. 9, p. e892998093, 2020. DOI: 10.33448/rsd-v9i9.8093. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/8093. Acesso em: 14 nov. 2024.

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Health Sciences