Different inoculating, forms of inoculation and their effects on the agronomic characteristics of soy culture

Authors

DOI:

https://doi.org/10.33448/rsd-v9i10.8499

Keywords:

Azospirillum brasilense; Bradyrhizobium; Co-inoculation; Nitrogen; Furrow.

Abstract

Biological nitrogen fixation replaces, at least in part, nitrogen fertilization and reduces production costs. Normally seeds are inoculated with bacteria, but there is technology to apply inoculants also in the sowing furrow, which allows increasing the possibilities of inoculation. In this work, it was evaluated the inoculation of different bacteria, applied alone or jointly, via furrow or seed on agronomic characteristics and soybean nitrogen extraction, in an area with a history of crop production. The experiment was a randomized block design in a 2x3 + 2 factorial scheme: seed or furrow application; Bradyrhizobium only, Azospirillum brasilense only and co-inoculation Bradyrhizobium + Azospirillum brasilense; two more witnesses (no inoculation). With seed inoculation, the lowest insertion heights of the first pod were verified with Bradyrhizobium and with co-inoculation, in furrow the control showed the highest result. Co-inoculation in seed presented the largest amount of pods per plant, when the application was in furrow the inoculation with Bradyrhizobium and co-inoculation stood out. The largest amount of grains per pod was obtained by inoculation with Bradyrhizobium and co-inoculation in seed, no differences were found in furrow. The largest mass of one thousand grains and productivity were obtained by inoculation with Bradyrhizobium and co-inoculation, regardless of the application form. Bradyrhizobium treatment and co-inoculation extracted more nitrogen in seed application, for the furrow the best result was with co-inoculation.

References

AOAC, Association of Official Analytical Chemists. Official methods of analysis. (2016). (20 ed.), Aoac International, Arlington 3100p.

Araújo, R. S., P. da Cruz, E. L. Souchie, T. N. Martin, A. S. Nakatani, M. A. Nogueira & M. Hungria. (2017). Preinoculation of Soybean Seeds Treated with Agrichemicals up to 30 Days before Sowing: Technological Innovation for Large-Scale Agriculture. International Journal of Microbiology, 4, 1-11.

Aung, T. T., P. Tittabutr, N. Boonker, D. Herridge & N. Teaumroong. (2013). Co-Inoculation Effects of Bradyrhizobium japonicum and Azospirillum sp. on Competitive Nodulation and Rhizosphere Eubacterial Community Structures of Soybean under Rhizobia-Established Eoil Conditions. African Journal of Biotechnology, 12, 2850-2862.

Bárbaro, I. M., M. A. P. C., Centurion, A. O. Di Mauro, S. H. Unêda-Trevisoli, N. H. C. Arriel & M. M. Costa. (2006). Path analysis and expected response in indirect selection for grain yield in soybean. Crop Breeding and Applied Biotechnology, 6, 151-159.

Bashan, Y. & Bashan, L. E. (2010). How the plant growth-promoting bacterium Azospirillum promotes plant growth - a critical assessment. Advances in agronomy, 108, 77-136.

Bhering, S. B., Santos, H. G. dos, Manzatto, C. V., Bognola, I. A., Fasolo, P. J., Carvalho, A. P. de, Potter, R. O.; Curcio, G. R. (2007). Mapa de Solos do Estado do Paraná. Rio de Janeiro: Embrapa Solos, 2007, 73p. (Documentos, 96).

Bhering, L. L. (2017). Rbio: A tool for biometric and statistical analysis using the R platform. Crop Breeding and Applied Biotechnology, 17, 187-190.

Braccini, A. L., G. E. G., Mariucci, A. K., Suzukawa, L. H. da S., Lima & G. G. Piccinin. (2016). Co-inoculação e modos de aplicação de Bradyrhizobium japonicum e Azospirillum brasilense e adubação nitrogenada na nodulação das plantas e rendimento da cultura da soja. Scientia Agraria Paranaensis, 15, 27-35.

Brasil, Ministério da Agricultura, Pecuária e Abastecimento. (2009). Regras para Análise de Sementes. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Mapa/ACS, Brasília, 395p.

Benintende, S., W. Uhrich, M. Herrera, F. Gangge, M. Sterren & B. Benintende. (2010). Comparación entre Coinoculación con Bradyrhizobium japonicum y Azospirillum brasilense e Inoculación simple con Bradyrhizobium japonicum en la Nodulación, Crecimiento y Acumulación de N en el Cultivo de Soja. Agriscientia, 27, 71-77.

Campo, R. J., Araujo, R. S. & M. Hungria. (2009). Nitrogen fixation with the soybean crop in Brazil: Compatibility between seed treatment with fungicides and bradyrhizobial inoculants. Symbiosis, 48, 154-163.

Campo, R. J., Araujo, R.S., F. L. Mostasso, & M. Hungria. (2010). In-furrow inoculation of soybean as alternative to fungicide and micronutrient seed treatment. Revista Brasileira de Ciência do Solo, 34, 103-1112.

Chibeba, A. M., Guimarães, M. F.; Brito, O.R., Nogueira, M.A., Araujo, R.S. & M. Hungria. (2015). Co-inoculation of soybean with Bradyrhizobium and Azospirillum promotes early nodulation. American Journal of Plant Science, 6,1641-1649.

Conab, Companhia Nacional de Abastecimento. (2019). Acompanhamento da safra brasileira de grãos: nono levantamento. Brasília: CONAB, 47p.

Dalchiavon, F. C. & M. P. Carvalho. (2012). Correlação linear e espacial dos componentes de produção e produtividade da soja. Semina: Ciências Agrárias, 33, 541-552.

Embrapa, Empresa Brasileira de Pesquisa Agropecuária. (2013). Tecnologias de Produção de Soja - Região Central do Brasil 2014. Londrina: Embrapa Soja. 259 p.

Fipke, G. M., Conceição, G.M., Grando, L.F.T., Ludwig, R. L., Nunes, U. R. & T. N. Martin. (2016). Co-inoculation with diazotrophic bacteria in soybeans associated to urea topdressing. Ciência e Agrotecnologia, 40, 522-533.

Hungria, M., Campo, R. J. & I. C. Mendes. (2007). A importância do processo de fixação biológica do nitrogênio para a cultura da soja: componente essencial para a competitividade do produto brasileiro. Londrina: Embrapa soja.80p. (Documentos 283).

Hungria, M., Nogueira, M.A. & R. S. Araújo. (2013). Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability. Biology and Fertility of Soils, 49, 791-801.

Hungria, M.; Nogueira, M. A. & R.S. Araújo. (2015a). Soybean Seed Co-Inoculation with

Bradyrhizobium spp. and Azospirillum brasilense: A New Biotechnological Tool to Improve Yield and Sustainability. American Journal of Plant Sciences, 6, 811-817.

Hungría, M., Nogueira M.A. & R.S. Araujo. (2015b). Alternative methods of soybean inoculation to overcome adverse conditions at sowing. African Journal of Agricultural Research, 23, 2329-2338.

Iapar, Instituto Agronômico do Paraná. Cartas climáticas do Paraná. 2012. Recuperado de http://www.iapar.br./modules/conteudo/conteudo.php?conteudo=597

Kintschev, M. R., Goulart, A. C. P. & F. M. Mercante. (2014). Compatibilidade entre a inoculação de rizóbios e fungicidas aplicados em sementes de feijoeiro-comum. Summa Phytopathologica, 40, 338-346.

Machineski, G. S., Scaramal, A.S., Matos, M. A. de, Machineski, O. & A. Colozzi Filho. (2018). Efficiency of pre-inoculation of soybeans with Bradyrhizobium up to 60 days before sowing. African Journal of Agricultural Research, 13, 1233-1242.

Marschner, H. (2011). Marschner's mineral nutrition of higher plants, (3 ed.), Academic press, 672p.

Masciarelli, O., Urbani, L., Reinoso, H. & V. Luna. (2013). Alternative mechanism for the evaluation of indole-3-acetic acid (IAA) production by Azospirillum brasilense strains and its effects on the germination and growth of maize seedlings. Journal of microbiology, 51, 590-597.

Meert, L., Fernandes, F. B., Müller, M. M. L., Rizzardi, D. A., Espindola, J. de S. (2020). Inoculação e coinoculação com Bradyhizobium japonicum e Azospirillum

brasilense na cultura da soja. Cultura Agronômica, 29, 118-129.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da

pesquisa científica. Brasil. Recuperado de http://repositorio.ufsm.br/handle/1/15824

Saharan, B. & V. Nehra. (2011). Plant Growth Promoting Rhizobacteria: A Critical Review. Life Science and Medical Research, 21, 1-30.

Schneider, F., Panizzon, L.C., Sordi, A., Lajú, C.R., Cericato, A. & C. Klein. (2017). Eficiência agronômica da cultura da soja (Glycine max (L.) Merril) submetida a coinoculação. Revista scientia agraria, 18, 72-79.

Souza, R. de, Ambrosini, A. & L. M. P. Passaglia. (2015). Plant growth-promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology, 38, 401-419.

Thomas, A. L. & J. A. Costa. (2010). Desenvolvimento da planta de soja e potencial de rendimento de grãos. In: Thomas, A. L., Costa, J. A. (Org.). Soja: manejo para alta produtividade de grãos. Porto Alegre: Evangraf.

Vieira Neto, S. A., Pires, F. R., Menezes, C. C. E., Menezes, J. F. S., Silva, A. G., Silva, G. P. & R. L. Assis. (2008). Formas de aplicação de inoculante e seus efeitos sobre a nodulação da soja. Revista Brasileira de Ciência do Solo, 32, 861-870.

Zilli, J. E., K. G. Ribeiro, R. J. Campo, & M. Hungria. (2009). Influence of fungicide seed treatment on soybean nodulation and grain yield. Revista Brasileira de Ciência do Solo, 33, 917-923.

Zilli, J. E., V. Gianluppi, R. J. Campo, J. R. C. Rouws & M. Hungria. (2010). Inoculação da soja com Bradyrhizobium no sulco de semeadura alternativamente à inoculação de sementes. Revista Brasileira de Ciência do Solo, 34,1875-1881.

Zuffo, A. M., A. T. Bruzi, P. M. de Rezende, M. C. Bianchi, E. V. Zambiazzi, I. O. Soares, A. B. M. Ribeiro & G. L. D. Vilela. (2016). Morphoagronomic and productive traits of RR® soybean due to inoculation via Azospirillum brasilense groove. African Journal of Microbiology Research,10, 438-444.

Published

26/09/2020

How to Cite

MEERT, L.; MULLER, M. M. L. .; GENÚ, A. M. .; ESPINDOLA, J. de S. .; ARAGÃO, G. N.; FIGUEIREDO, A. S. T. . Different inoculating, forms of inoculation and their effects on the agronomic characteristics of soy culture. Research, Society and Development, [S. l.], v. 9, n. 10, p. e2969108499, 2020. DOI: 10.33448/rsd-v9i10.8499. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/8499. Acesso em: 18 nov. 2024.

Issue

Section

Agrarian and Biological Sciences