Study of quantification pf phenolic compounds, evaluation of antioxidant and antimicrobial activities of the stem bark of the Croton cajucara BENTH
Keywords:Croton cajucara BENTH; Extraction; Natural product.
The main objective of this work was to quantify the phenolic compounds, and to evaluate antioxidant and antimicrobial the activity of Croton cajucara BENTH stem bark extracts. An experimental planning (Box Hunter and Hunter) of two factors and two levels determine which variables influenced in the extraction process was used. To the quantification of phenolic compounds was used the methodology of Folin-Ciocalteau. To the antioxidant activity the IC50 method was applied. For the antimicrobial analysis, the microdilution method was employed to indicate the Minimum Inhibitory Concentration (MIC) against the bacterial strains, as well as fungal strain. In the quantification of phenolic compounds, there was a variation between 26.98 and 50.32 mgEAG /g. Regarding antioxidant activity, values from 9.01% to 14.03% were found. For antimicrobial activity, the extracts did not inhibit the growth of the tested bacteria and fungi. All the results were treated statistically and, in general, proved that the best extraction conditions were T = 60 °C and G = 0.10 mm.
Agência nacional de vigilância sanitária. (2010). Farmacopéia brasileira. Brasília: Autor.
Akhanazarova, S. & Kafarov, V. (1982). Experiment Optimization on Chemistry and Chemical Engineering. Mir Publishers.
Andrade, S. F., Cardoso, L. G. & Bastos, J. K. (2007). Anti-inflammatory and antinociceptive activities of extract, fractions and populnoic acid from bark wood of Austroplenckia populnea. Journal of Ethnopharmacoly. 109(3), 464-471. DOI: https://doi.org/10.1016/j.jep.2006.08.023
Araújo, E. C., et al. (2007). Use of medicinal plants by patients with cancer of public hospitals in João Pessoa (PB). Revista Espaço para a Saúde, 8(2), 44-52. Recuperado de: https://pesquisa.bvsalud.org/portal/resource/pt/lil-464830
Bagatini, M. D., Silva, A. C. F. & Tedesco, S. B. (2007). Uso do sistema teste de Allium cepa como bioindicador de genotoxicidade de infusões de plantas medicinais. Rev Bras Farmacogn, 17(3), 444-447. DOI: http://dx.doi.org/10.1590/S0102-695X2007000300019
Botterweck, A. A. M, et al. (2000). Intake of butylated hydroxyanisole and butylated hydroxytoluene and stomach cancer risk: results from analyses in the Netherlands cohort study. Food and Chemical Toxicology, 38(7), 599-605. DOI: https://doi.org/10.1016/S0278-6915(00)00042-9
Bouchet, N, Barrier, L & Fauconneau, B. (1998). Radical scavenging activity and antioxidant properties of tannins from Guiera senegalensis (Combretaceae). Phytotherapy research, 12(3), 159-162. DOI: https://doi.org/10.1002/(SICI)1099-1573(199805)12:3<159::AID-PTR209>3.0.CO;2-C
Brasil, D. S. B. B., Souza, A. A. M., Rodrigues, C. C., Costa, C. M. L. C., Santana, L. B. & Santana, E. B. (2019). Revista de propriedade intelectual (2504 ed.), Brasília: Instituto nacional de propriedade industrial.
Cavin, A., et al. (1998). Use of on-flow LC/1 H NMR for the study of an antioxidant fraction from Orophea e nneandra and isolation of a polyacetylene, lignans, and a tocopherol derivative. Journal of natural products, 61(12), 1497-1501. DOI: https://doi.org/10.1021/np980203p
Dahanukar, S. A., et al. (2000). Pharmacology of medicinal plants and natural products. Indian journal of pharmacology, 32(4), 81-118. Recuperado de: https://scholar.google.com.br/scholar?hl=pt-BR&as_sdt=0%2C5&q=Pharmacology+of+medicinal+plants+and+natural+products&btnG=
Decker, E. A. (2008). Food lipids: chemistry, nutrition, and biotechnology (4th ed). CRC Press.
Derringer, G. & Suich, R. (1980). Simultaneous optimization of several variables. Journal of Quality Technology, 12(4), 214-219. DOI: https://doi.org/10.1080/00224065.1980.11980968
Di stali, L. C., et al. (1988). Screening in mice of some medicinal plants used for analgesic purposes in the state of Sao Paulo. Journal of ethnopharmacology, 24(2-3), 205-211. DOI: https://doi.org/10.1016/0378-8741(88)90153-5
Eloff, J. N. (1998). A sensitive and quick microplate method to determine the minimalinhibitory concentration of plant extracts for bacteria. Planta Médica, 64, 711-713. Recuperado de: https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2006-957563
Gutfinger, T. (1981). Polyphenols in olive oils. Journal of the American Oil Chemists' Society, 58(11), 966-968. DOI: https://doi.org/10.1007/BF02659771
Matkowski, A. (2008). Plant in vitro culture for the production of antioxidants – A review. Biotechnology Advances, 26(2), 548-560. DOI: https://doi.org/10.1016/j.biotechadv.2008.07.001
Rauha, J. P. (2000). Antimicrobial effects of finish plant extracts containing flavonoids and other phenolic compounds. International Journal of Food Microbiology, 56(1), 3-12. DOI: https://doi.org/10.1016/S0168-1605(00)00218-X
Reische, D.W., Lillard, D. A. & Eitenmiller, R. R. (2008). Food lipids: chemistry, nutrition, and biotechnology. CRC Press.
Scopel, M. (2005). Análise Botânica, Química e Biológica Comparativa entre Flores das Espécies Sambucus nigra L. e Sambucus australis Cham. & Schltdl. e Avaliação Preliminar da Estabilidade. Universidade Federal do Rio Grande do Sul, Rio Grande do Sul. Recuperado de: https://www.lume.ufrgs.br/handle/10183/6559
Sousa, C. M. M, et al. (2007). Fenóis totais e atividade antioxidante de cinco plantas medicinais. Química nova, 30(2), 351-355. DOI: https://doi.org/10.1590/S0100-40422007000200021
Wayne, P. A. (2007). Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing, 1(1), 100-121. Recuperado de: https://scholar.google.com/scholar?q=CLSI.+Performance+standards+for+antimicrobial+susceptibility+testing.+17+th++Informational+Supplement.+M100-S17+2007+Wayne,+PA+Clinical+and+Laboratory+Standards+Institute+
How to Cite
Copyright (c) 2020 Alexandre Augusto Moraes de Souza; Davi do Socorro Barros Brasil; Cintya Cordovil Rodrigues; Sônia Maria de Freitas Almeida; Nayara Maria Monteiro da Silva; Maria Fani Dolabela; Dayse Lúcia do Nascimento Brandão; Luciane do Socorro Nunes dos Santos Brasil
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.