Biocompatibility assessment of bioceramic repair cements: An in vivo study in wistar rats
DOI:
https://doi.org/10.33448/rsd-v10i7.14422Keywords:
Pulpotomy; Inflammation; Materials testing; Endodontics.Abstract
The aim of this study was to evaluate the biocompatibility of Biodentine® bioceramic repair cement when compared to White Angelus MTA® and calcium hydroxide. Twenty-four Wistar rats were used (n = 6 animals / group), divided into four experimental periods of 7, 15, 30 and 60 days. The animals received subcutaneous implants of polyethylene tubes containing the 3 test materials and an empty tube used as control. After experimental periods, animals were euthanized and the tubes were removed along with the adjacent surrounding tissue and histologically processed to assess biocompatibility. Inflammatory infiltrate and fibrous capsule thickness were analyzed by HE staining through inflammation scores. Data was submitted to Kruskal Wallis and Dunn’s test (p <0.05). After 15 days, Biodentine® induced less inflammatory response than control group and Ca (OH)2 (p <0.05). In calcium hydroxide group there was a decrease in the inflammatory infiltrate, as well as a decrease in the fibrous capsule, from day 15 to 60. The White Angelus MTA® showed thin fibrous capsule and low inflammatory intensity in all periods. At the endo of experiment, all materials showed mild inflammation and thin fibrous capsule. It is concluded that Biodentine® demonstrated tissue biocompatibility as it induced low inflammation, which reduced over time, comparable White Angelus MTA® and calcium hydroxide.
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Copyright (c) 2021 Diego Valentim; Carlos Roberto Emerenciano Bueno; Vanessa Abreu Sanches Marques; Francine Benetti; Ana Maria Veiga Vasques; Marina Tolomei Sandoval Cury; Ana Claudia Rodrigues da Silva; Rogério Castilho Jacinto; Gustavo Sivieri-Araujo; Luciano Tavares Angelo Cintra; Eloi Dezan-Junior
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