Antimicrobial sensitivity profile of isolated bacteria, from piometra in bitches, in front of uterine infusion gentamycin (Gentrin®)
DOI:
https://doi.org/10.33448/rsd-v10i7.15170Keywords:
Reproduction; Reproductive biotechnology; Alternative protocol.Abstract
The hyperplastic cystic endometrial - pyometra complex is characterized by a hormonal disorder, with progesterone as the protagonist, leading to the appearance of ovarian cysts and a sequence of changes in the endometrium, followed by bacterial colonization, with Escherichia and Staphylococus being among the main ones, being thus, the objective was to isolate them and evaluate their sensitivity to Gentrin® in three different concentrations (150mg, 200mg and 300mg), with Ceftriaxone as control. Ten female dogs were used for this research, with an average age of 7 years, after collecting the secretions of pyometra, Escherichia and Staphylococus were isolated from them for culture and antibiogram. They were isolated from E. coli in 60% of the samples and Staphylococus spp. in 40%, in the antibiogram tests both demonstrated 100% sensitivity to Gentrin® of 300mg, and in smaller percentages to 150mg and 200mg. This concludes the effectiveness of Gentrin® 300mg in the treatment by uterine infusion for pyometra.
References
Arispe, G. F. L., et al. (2013). Sensitivity and resistance on the antibiogram of Staphylococcus aureus in patients of the Hospital Clinical Viedma. Ver. Cient. Cienc. Méd. 16(2).
Álvarez, C. E. T. (2018). Determinación de la presencia de Staphylococcus spp. coagulasa positivo y sus patrones de resistencia a antibióticos en casos de piodermatitis canina en la clínica veterinaria FMVZ-UCE. 2018. 59 f. Dissertação (Trabajo de titulación previo a la obtención del Título de Médico Veterinario y Zootecnista) - Carrera de Medicina Veterinaria y Zootecnia. Quito.
Bauer, A. W., Kirby, W. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal Clinical Pathology, 45, 493-496.
Barsanti, J. A. (2012). Genitourinary infections. In: C. E. Greene (Elsevier), editor. Infectious Diseases of the Dog and Cat. 1. 4th ed. (pp. 1376). Georgia.
Carlos, C., et al. (2012). Use of Escherichia coli BOX-PCR fingerprints to identify sources of fecal contamination of water bodies in the State of Sao Paulo, Brazil. Journal of Environmental Management. 93(1), 38–43.
Carmona, J. U., López, C. & Giraldo, C. E. (2008). Pleural abscesses produced by Staphylococcus aureus and beta-haemolytic Streptococcus spp. as a sequel of pleuropneumonia in a horse. A case report. Revista Colombiana de Ciências Pecuárias. 590-596.
Chen, Y. M. M. et al. (2003). Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches. Veterinary Microbiology. 94(1), 57-69.
Coggan, J. A. (2005). Estudo microbiológico de conteúdo intra-uterino e histopatológico de útero de cadelas com piometra e pesquisa de fatores de virulência em cepas de E. coli e o potencial à saúde humana. 157 f. Dissertação (mestrado) – Universidade de São Paulo. Medicina Veterinária e Zootecnia.
Coggan, J. A., et al. (2008). Microbiological and histopathological aspects of canine pyometra. Brazilian Journal of Microbiology, 39(3), 477–483.
Cyrino, M. A. (2020). Biomarcadores inflamatórios e do estresse oxidativo na piometra canina. 82 f. Dissertação (mestrado) - Universidade Estadual Paulista "Júlio de Mesquita Filho", Faculdade de Medicina Veterinária e Zootecnia, Botucatu.
Guardabassi, L., Schwarz, S. & Lloyd, D. H. (2004). Pet animals as reservoirs of antimicrobial-resistant bactéria. Journal of Antimicrobial Chemotherapy, 54(2), 321–332.
Hagman, R. (2018). Pyometra in small animals. Veterinary Clinics: Small Animal Practice, 48(4), 639-661.
Hagman, R. & Greko, C. (2005). Antimicrobial resistance in Escherichia coli isolated from bitches with pyometra and from uterine sample from Other dogs. The Veterinary record, 157, 193-196. 10.1136/vr.157.7.193
Hagman, R., et al. (2006). Differentiation between pyometra and cystic endometrial hyperplasia/mucometra in bitches by prostaglandin F2α metabolite analysis. Theriogenology, 66(2), 198-206.
Huber, H., et al. (2013). ESBL-producing uropathogenic Escherichia coli isolated from dogs and cats in Switzerland. Veterinary Microbiology, 162(2-4), 992–996.
Inoue, I., Shibata, S. & Fukata, T. (2013). Efficacy of fosfomycin on Escherichia coli isolated from bitches with pyometra. The Journal of Veterinary Medical Science, 75(5), 657–658.
Jitpean, S., et al. (2014). Outcome of pyometra in female dogs and predictors of peritonitis and prolonged postoperative hospitalization in surgically treated cases. BMC Veterinary Research, 10(6), 1746-6148.
Kida, K., et al. (2010). Epidermal growth factor, transforming growth factor-α, and epidermal growth factor receptor expression and localization in the canine endometrium during the estrous cycle and in bitches with pyometra. Theriogenology, 73(1), 36-47.
Lobato-miguélez, J., San-roman-sigler, M. & López-valverde, M. (2014). Piometra senil benigno gigante en útero bicorne. Medisur, 12(2), 414-418.
Niskanen, M., Thrusfield, M. V. (1998). Associations between age, parity, hormonal therapy and breed, and pyometra in Finnish dogs. Veterinary Record, 143(18), 493-498.
Olson, P. N. S., Mather, E. C. (1978). Canine vaginal and uterine bacterial flora. Jornal of American Veterinary Medical Association, 172(6), 708-711.
Padilha, L. C. P. (2010). Análise do perfil bioquímico e hematológico em fêmeas caninas acometidas por piometra e cultura e antibiograma do conteúdo intrauterino. 2010. 53 f. Tese (Conclusão de Residência Médico Veterinária em Reprodução Animal e Obstetrícia Veterinária) - Universidade Estadual Paulista, Jaboticabal.
Pinchetti, M. A. A., Crossley, R. & Maier, L. (2011). Flora bacteriana y sensibilidad microbiana de cepas aisladas a partir de úteros de perras con piometra. REDVET Revista Electrónica de Veterinaria, 12(9), 1-6.
Pretzer, S. D. (2008). Clinical presentation of canine pyometra and mucometra: a review. Theriogenology, 70(3), 359-363.
Ribeiro, S. L. (2019). Injúria renal aguda em cadelas com piometra: a importância do diagnóstico precoce. 2019. 53 f. Dissertação (mestrado em Ciência Animal) – Escola de Veterinária e Zootecnia da Universidade Federal de Goiás.
Ros, L., Holst, B. S. & Hagman, R. (2014). A retrospective study of bitches with pyometra, medically treated with aglepristone. Theriogenology, 82(9), 1281-1286.
Siqueira, A. K., Ribeiro, M. G. & Salerno, T. (2008). Perfil de sensibilidade e multirresistência em linhagens de Escherichia coli isoladas de infecção do trato urinário, de piometra e de fezes de cão. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 60(5), 1263–1266.
Smith, F. O. (2006). Canine pyometra. Theriogenology, 66, 610-612.
Trautwein, L. G. C., et al. (2017). Piometras em cadelas: relação entre o prognóstico clínico e o diagnóstico laboratorial. Ciência Animal Brasileira, 18, 1-10.
Van den bogaard, A. E., Stobberingh, E. E. (2000). Epidemiology of resistance to antibiotics: links between animals and humans. International Journal of Antimicrobial Agents, 14(4), 327–335.
Verstegen, J., Dhaliwal, G. & Verstegen-onclin, K. (2008). Mucometra, cystic endometrial hyperplasia, and pyometra in the bitch: advances in treatment and assessment of future reproductive success. Theriogenology, 70(3), 364-374.
Whitehead, M. L. (2008). Risk of pyometra in bitches treated for mismating with low doses of oestradiol benzoate. Veterinary Record, 162(23), 746-749.
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