Biometrics and nutritional status of white oat (Avena sativa L.) culture under Bacillus subtilis and B. megaterium inoculation

Authors

DOI:

https://doi.org/10.33448/rsd-v10i5.15270

Keywords:

Agriculture; Biology; Soil fertility; Microorganisms.

Abstract

The aim of this work was to evaluate responses of Avena sativa L. to inoculation, with isolates of Bacillus subtilis and B. megaterium.The experiment was carried out on soil classified as dystrophic Red Latosol in the municipality of Campo Grande, MS. The treatments consisted of five doses of inoculant (0, 50, 100, 150, 200 mL), applied in the planting furrow, being distributed in experimental plots, with the dimensions of 3.0 mx 4.0 m, according to the outline in random blocks with four repetitions. The biometric parameters of the plant were evaluated: tiller diameter and tiller quantity, fresh and dry shoot biomass production, grain production. The quality of the product evaluated the bromatology and the mineral content of the flag leaf. Oat plants showed an increase in growth rate (TC), tiller diameter (DP), leaf contents of N, Fe and Cu, crude protein (PB), neutral detergent fiber (NDF). The performance of oats was favored with doses of inoculant up to 125 mL. It was also observed an increasing of protein concentration in the plant in a concentration-response fashion. It is concluded that the inoculant based on the two isolates of the bacteria used in the planting furrow, benefits the production of oats.

References

Anupa, N., Asha, J. A. & Sanjeev, S. K. (2007). Production and Characterization of Siderophores and its Application in Arsenic Removal from Contaminated Soil. Water Air and Soil Pollution, 180(4), 1999-2012. https://doi.org/10.1007/s11270-006-9263-2.

Araujo, F. F. & Hungria, M. (1999). Nodulação e rendimento de soja, co-infectada com Bacillus subtilis, Bradyrhizobium japonicum/ Bradyrhizobium elkanii. Pesquisa Agropecuária Brasileira, 34(5), 1633-1643. https://doi.org/10.1590/S0100-204X1999000900014.

Bergey, D. H., & Holt, J. G. (2000). Bergey's manual of determinative bacteriology. (9th ed.), Lippincott Williams & Wilkin. https://doi.org/10.1038/162833a0.

Castro, J. R. P. (2019). Panorama do Uso de Inoculantes no Brasil. Perspectivas e Desafios. In: XIX RELARE, 2019, Foz do Iguaçu. Anais... Foz do Iguaçu: ANPII. 21-24.

Cecato, U., Rêgo, F. C. A., Gomes, J. A. N. Canto, M. W, Jobim, C. C, Coneglian, S. & Moreira, F. B. (2001). Produção e composição química em cultivares e linhagens de aveia (Avena spp.). Acta Scientiarum, 23(4), 775-780. https://doi.org/10.4025/actascianimsci.v23i0.2578.

CEMTEC – Centro de monitoramento de Tempo, do Clima e dos Recursos Hídricos de Mato Grosso do Sul, Campo Grande– MS, http://www.cemtec.ms.gov.br/.

Demétrio, J. V., Costa, A. C. T. & Oliveira, P. S. R. (2012). Produção de biomassa de cultivares de aveia sob diferentes manejos de corte. Pesquisa Agropecuária Tropical, 42(2)2, 198-205. https://doi.org/10.1590/S1983-40632012000200011.

EMBRAPA. Empresa Brasileira de Pesquisa Agropecuária. (2009). Manual de análises químicas de solos, plantas e fertilizantes. Fábio Cesar da Silva (Ed. Técnico) (2a ed.), Embrapa Informação Tecnológica, 627.

Fedrizzi, S. M. G. (2006). Produção de metabólitos antimicrobianos e sideróforos de isolados provenientes de Terra Preta Antropogênica da Amozônia Ocidental. 117pp. Tese (Doutorado em Ciências) - Universidade de São Paulo. 10.11606/T.64.2006.tde-20032007-081838.

Glick, B. R., Cheng, Z., Czarny, J. & Duan, J. (2007). Promotion of plant growth by ACC deaminase - producing soil bacteria. European Journal of Plant Pathology, 119(3), 329-339. https://doi.org/10.1080/07352680701572966.

Hernández-León, R., Rojas-Solís, D., Contreras-Pérez, M., Orozco-Mosqueda, M. C., Macías-Rodríguez, L. I., Reyes, L. C. H., Valencia-Cantero, C. E. & Santoyo, G. (2015). Characterization of the antifungal and plant growth - promoting effects of diffusible and volatile organic compounds produced by Pseudomonas fluorescens strains. Biological Control. 81(1), 83-92. https://doi.org/10.1016/j.biocontrol.2014.11.011.

Holden, L. A. (1999). Comparison of methods of in vitro dry matter digestibility for ten feeds. Journal of Dairy Science, 82(8), 1791-1794. https://doi.org/10.3168/jds.S0022-0302(99)75409-3.

Hood, M. I. & Skaar, E. P. (2012). Nutritional immunity: transition metals at the pathogen - host interface. Nature Reviews Microbiology, 10(8), 525-537. https://doi.org/10.1038/nrmicro2836.

Hoppo, S. D., Elliot, D. E. & Reuter, D. J. (1999). Plant tests for diagnosing phosphorus deficiency in barley (Hordeum vulgare L.). Australian Journal of Experimental Agriculture, 39, 857-872. https://doi.org/10.1071/EA99029.

Kavitha, J., Jonathan, E. I. & Umamaheswari, R. (2007). Field application of Pseudomonas fluorescens, Bacillus subtilis and Trichoderma viride for the control of Meloidogyne incognita in sugarbeet. Journal of Biological Control, 21(2), 211-215. https://doi.org/10.18311/JBC/2007/3851.

Kumar, K. V. K., Yellareddygari, S. K. R., Reddy, M. S., Kloepper, J. W., Lawrence, K. S., Zhou, X. G., Sudini, H., Groth, D. E., Raju, S. K. & Miller, M. E. (2012). Efficacy of Bacillus subtilis MBI 600 against shealth blight caused by Rhizoctonia solani and on growth and yield of rice. Rice Science, 19(1), 55-63. https://doi.org/10.1016/S1672-6308(12)60021-3.

Luz, W. C. (2001). Evaluation of plant growth - promoting and bioprotecting rhizobacteria on wheat crop. Fitopatologia Brasileira, 26(3), 597-600. https://doi.org/10.1590/S0100-41582001000300003.

Malavolta, E., Vitti, G. C. & Oliveira, S. A. de. (1997). Avaliação do estado nutricional das plantas: princípios e aplicações. POTAFOS.

Manjula, K. & Podile, A. R. (2005). Increase in Seedling Emergence and Dry Weight of Pigeon Pea in the Field with Chitin-Supplemented Formulations of Bacillus subtilis AF 1. World Journal of Microbiology and Biotechnology, 21(7), 1057–1062. https://doi.org/10.1007/s11274-004-8148-z.

Mengel, K. & Kirkby, E. A. (2001). Effect of split application of potassium on growth, yield and potassium accumulation by soybean. Fertility Research, 39(3), 217-222. https://doi.org/10.1007/BF00750249.

Mundstock, C. M. & Bredemeier, C. (2001). Disponibilidade de nitrogênio e sua relação com o afilhamento e o rendimento de grãos de aveia. Ciência Rural, 31(2), 205-211. https://doi.org/10.1590/S0103-84782001000200003.

Nakagawa, J., Crusciol, C. A. C. & Zucareli, C. (2009). Teores de nutrientes da folha bandeira e grãos de aveia-preta em função da adubação fosfatada e potássica. Semina: Ciências Agrárias, 30(4), 833-840. https://doi.org/10.1590/S0006-87052005000300014.

Nicholson, W. L., Mu-Nakata, N., Horneck, G., Melosh, H. J. & Setlow, P. (2000). Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and Molecular. Biology Reviews, 64(3) 548-572. https://doi.org/10.1128/MMBR.64.3.548-572.2000.

Novais, R. F., Smyth, T. J. & Nunes, F. N. (2007). Fósforo, I. N. Fertilidade do solo, ed. Novais, R. F., Alvarez V. V. H., Barros, N. F. de, Fontes, R. L. F., Cantarutti, R. B., & Lima, J. C. Sociedade Brasileira de Ciência do Solo. https://www.locus.ufv.br/bitstream/123456789/14671/1/12.pdf.

Oliveira, A. L. M., Urquiaga, S. & Baldani, J. I. (2003). Processos e mecanismos envolvidos na influência de microrganismos sobre o crescimento vegetal. Embrapa Agrobiologia, 161(40). http://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAB-2010/28064/1/doc161.pdf.

Ongena, M., Duby, F., Jourdan, E., Beaudry, T., Jadin, V., Dommes, J. & Thonart, P. (2005). Bacillus subtilis M4 decreases plant susceptibility towards fungal pathogens by increasing host resistance associated with differential gene expression. Applied Microbiology and Biotechnology, 67(5) 692-698. https://doi.org/10.1007/s00253-004-1741-0.

Pantano, G., Grosseli, G. M., Mozeto, A. A. & Fadini, P. S. (2016). Sustentabilidade no uso do fósforo: uma questão de segurança hídrica e alimentar. Química Nova, 39(1), 732-740. https://doi.org/10.5935/0100-4042.20160086.

Pei-Xiang, Y., Li, M. A., Ming-Hui, C., Jia-Quin, X., Feng, H. E., Chang-Qun, D., Ming-He, M., Dun-Huang, F., Yan-Qing, D. & Fa-Xiang, Y. (2012). Phosphate solubilizing ability and phylogenetic diversity of bacteria from phosphorus rich soils around Dianchi lake drainage area of China. Pedosphere, 22(5), 707-716. https://doi.org/10.1016/S1002-0160(12)60056-3.

Potot, S., Serra, C. R., Henriques, A. O. & Schyns, G. (2010). Display of recombinant proteins on Bacillus subtilis spores, using a coat-associated enzyme as the carrier. American Society for Microbiology Journals. 76(17), 5926-5933. https://doi.org/10.1128/AEM.01103-10.

Primavesi, A. C., Primavesi, O., Cantarella, H., Godoy, R. & Vivaldi, L. J. (2002). Adubação de aveia em dois sistemas de plantio. Pesquisa Agropecuária Brasileira. 37(12), 1773-1778. https://doi.org/10.1590/S0100-204X2002001200014.

Quadros, P. D., Roesch, L. F. W., Silva, P. R. F., Vieira, V. M., Roehrs, D. D. & Camargo, F. A. O. (2014). Desempenho agronômico a campo de híbridos de milho inoculados com Azospirillum. Revista Ceres, 61(2), 209-218. https://doi.org/10.1590/S0034-737X2014000200008.

Reichard, P. U. (2005). Effects of microbial and plant siderophore ligands on the dissolution of iron oxides. 189. Tese (Doutorado em Ciências Naturais). Swiss Federal Institute of Technology, Zurich. https://doi.org/10.3929/ethz-a-004937932.

Ribeiro, A. C., Guimarães, P. T. G. & Alvarez, V. H. (1999). Recomendações para o uso de corretivos e fertilizantes em Minas Gerais - 5º Aproximação. Sociedade Brasileira de Ciência do Solo. 360.

Rineh, A., Kelso, J., Michael, V., Fatma, T., George, H. & Michael, P. (2014). Clostridium difficile infection: Molecular pathogenesis and novel therapeutics. Expert review of anti-infective therapy. 12(1), 131-50. https://doi.org/10.1586/14787210.2014.866515.

Rodriguez, H. & Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17(5), 319-339. https://doi.org/10.1016/S0734-9750(99)00014-2.

Saraf, M., Rajkumar, S. & Saha, T. (2011). Perspectives of PGPR in agri-ecosystems. Springer, 1(4), 361-385. https://doi.org/10.1007/978-3-642-18357-7_13.

SAS - Statistical Analysis System [SAS]. (2004). STAT user's guide: statistics. Version 9.1. Cary, NC: Statistical Analysis System Institute Inc., 1057 https://www.sas.com/pt_br/trials.html.

Silva, D. J. & Queiroz, C. (2006). Análise de alimentos: métodos químicos e biológicos. (3a ed.), UFV, 235.

Storey, S. P., Boghozian, R., Little, J. L., Lowman, D. W. & Chakraborty, R. (2006). Characterization of ‘Schizokinen’, a dihydroxamate - type siderophore produced by Rhizobium leguminosarum IARI 917. Biometals 19(6), 637-649. https://doi.org/10.1007/s10534-006-9001-7.

Tilley, J. M. A. & Terry, R. A. (1963). A two-stage technique for the in vitro digestion of forage crops. Journal British Grassland Society, 18(2), 104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x.

Van Soest, P. J., Robertson, J. D. & Lewis, B. A. (1965). Methods for dietary fiber, neutral detergent fiber, nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2.

Yang, J., Kloepper, J. W. & Ryu, C. M. (2009). Rhizosphere bacteria help plants tolerate abiotic stress. Trends in Plant Science, 14(1), 1-4. https://doi.org/10.1016/j.tplants.2008.10.004.

Zaidi, A., & Khan, M. S. (2006). Co-inoculation Effects of Phosphate Solubilizing Microorganisms and Glomus fasciculatum on Green Gram-Bradyrhizobium Symbiosis. Turkish Journal of Agriculture and Forestry, 30(3), 223-230. https://dergipark.org.tr/tr/pub/tbtkagriculture/issue/11621/138452.

Published

15/05/2021

How to Cite

SANTOS, A. F. dos; CORRÊA, B. O.; KLEIN, J.; BONO, J. A. M. .; PEREIRA, L. C.; GUIMARÃES, V. F.; FERREIRA , M. B. Biometrics and nutritional status of white oat (Avena sativa L.) culture under Bacillus subtilis and B. megaterium inoculation. Research, Society and Development, [S. l.], v. 10, n. 5, p. e53410515270, 2021. DOI: 10.33448/rsd-v10i5.15270. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/15270. Acesso em: 26 nov. 2024.

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Section

Agrarian and Biological Sciences