Clinical-pathological characterization and research of COX-2, CEA and CA15.3 in cats with breast cancer
DOI:
https://doi.org/10.33448/rsd-v11i4.27210Keywords:
Tumour; Carcinoma; Mammary gland; Felis catus.Abstract
Mammary gland neoplasms have a multifactorial origin and are classified as the main type of neoformation that affects cats. The diagnosis is obtained from histopathological analysis, where it is possible to determine the type and histological grade of the breast tumor. More recently, tissue biomarkers have allowed more accurate analyzes of the degree of malignancy of tumors, with an attempt to use serum markers to diagnose micronodules and metastases early. Thus, this study aimed to characterize the clinical profile and evaluate the serum levels of carcinoembryonic antigen (CEA) and carcinogen antigen 15.3 (CA15.3), quantified by ELISA. In addition, the objective was to define the histopathological diagnosis and identify the immunohistochemical profile of cyclooxygenase-2 (COX-2) in breast neoplasms. The study was carried out in 49 cats, 38.78% were not neutered and 59.18% had already been submitted to contraceptive hormones. Malignant neoplasms accounted for 76.92%, with the cribriform, tubulopapillary and tubular types being the most frequent. The mean serum levels of CA15.3 and CEA were 2.4108 ± 0.4748 U/mL and 0.0024 µg/L, respectively. The mammary tumor in cats is highly malignant, and more than one histological type can coexist in cats with multiple nodules. The immunoexpression of COX-2 and showed no significant association with the appearance of breast tumors. The use of tumor markers CEA and CA15.3 was not efficient in cats with mammary lesions.
References
Borrego, J. F., Cartagena, J. C. & Engel, J. (2009). Treatment of feline mammary tumours using chemotherapy, surgery and a COX-2 inhibitor drug (meloxicam): a retrospective study of 23 cases (2002–2007). Veterinary and Comparative Oncology, 7(4), 213-22. doi: 10.1111/j.1476-5829.2009.00194.x.
Campos, L. C. (2010). Avaliação de marcadores tumorais séricos em cadelas com e sem metástase em câncer de mama. (Dissertação de mestrado). Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
Campos, L. C., Lavalle, G. E., Estrela-Lima, A., Melgaço de Faria, J. C., Guimarães, J. E., Dutra, A. P., Ferreira, E., Sousa, L.P., Rabelo, E. M. L., Viera da Costa, A. F. D. & Cassali, G. D. (2012). CA15.3, CEA, and LDH in dogs with malignant mammary tumors. Journal of veterinary internal medicine, 26, 1383-88. doi: 10.1111/j.1939-1676.2012.01014.x.
Cassali, G.D., Campos, C. B., Bertagnolli, A. C., Lima, A. E., Lavalle, G. E., Damasceno, K. A., De Nardi, A. B., Cogliati, B., Costa, F. V. A., Sobral, R., Di Santis, G. W., Fernandes, C. G., Ferreira, E., Salgado, B. S., Vieira-Filho, C. H. C., Silva, D. N., Martins-Filho, E. F., Teixeira, S. V., Nunes, F. C. & Nakagaki, K. Y. R. (2018). Consensus for the diagnosis, prognosis and treatment of feline mammary tumors. Brazilian Journal of Veterinary Research and Animal Science, 55(2), 1-17. doi: 10.11606/issn.1678-4456.bjvras.2018.135084
Costa, M. M. (2010). Estudo epidemiológico e anatomopatológico de tumores mamários na cadela e na gata. (Dissertação de mestrado em Medicina Veterinária). Faculdade de Medicina Veterinária da Universidade Técnica de Lisboa, Lisboa, Portugal.
De Campos, C. B., Damasceno, K. A., Gamba, C. O., Ribeiro, A. M., Machado, C. J., Lavalle, G. E. & Cassali, G. D. (2016). Evaluation of prognostic factors and survival rates in malignant feline mammary gland neoplasms. Journal of Feline Medicine and Surgery, 18(12), 1003–1012. doi: 10.1177/1098612X15610367.
Elston, C. W. & Ellis, I. O. (1998). The breast. Systemic pathology. London: Churchill Livingstone.
Ferreira, E., Campos, M. R. A., Nakagaki, K. Y. R., Cassali, G. D. (2017). Marcadores prognósticos e preditivos no câncer de mama. In G. D. Cassali (Ed.), Patologia mamária canina: Do diagnóstico ao tratamento (pp. 141-49). São Paulo, SP: Medvet.
Gelaleti, G. B. (2011). Avaliação da interleucinas 8 e 12, do CA 15-3 e DNA livre circulante como marcadores prognósticos no soro sanguíneo de cadelas com neoplasia mamária. (Dissertação de mestrado em Genética). Universidade Estadual Paulista, São José do Rio Preto, Brasil.
Lavalle, G. E., Campos, C. B., Bertagnolli, A. C. & Cassali, G. D. (2012). Canine malignant mammary gland neoplasms with advanced clinical staging treated with carboplatin and cyclooxygenase inhibitors. In Vivo, 26(3), 375-79. Recuperado de https://iv.iiarjournals.org/content/26/3/375.long
Mata, L. C. C. (2017). Marcadores séricos no câncer de mama. In G. D. Cassali (Ed.). Patologia mamária canina: Do diagnóstico ao tratamento (pp. 189-92). São Paulo, SP: Medvet.
Mcneill, C. J., Sorenmo, K. U., Shofer, F. S., Gibeon, L., Durham, A. C., Barber, L. G., Baez, J. L. & Overley, B. (2009). Evaluation of adjuvant doxorubicin based chemotherapy for the treatment of feline mammary carcinoma. Journal of Veterinary Internal Medicine, 23(1), 123-29. doi: 10.1111/j.1939-1676.2008.0244.x.
Mendes, G. A., Sturmer, F. C. R. & Basegio, D. L. (2010). Utilização dos marcadores CA 15.3 e CEA no seguimento de pacientes com neoplasia mamária. NewsLab, 102, 120-26. Recuperado de https://www.researchgate.net/publication/281747781_Use_of_CA153_e_CEA_markers_in_the_follow_up_of_breast_cancer_patients
Misdorp, W., Else, R. W., Hellmén, E. & Lipscomb, T.P. (1999). International Histological Classification of Tumors of Domestic Animals. Washington, D.C: World Health Organization.
Pierce, B. L., Ballard-Barbash, R., Bernstein, L., Baumgartner, R. N., Neuhouser, M. L., Wener, M. H., Baumgartner, K. B., Gilliland, F. D., Sorensen, B. E., McTiernan, A. & Ulrich, C. M. (2009). Elevated biomarkers of inflammation are associated with reduced survival among breast cancer patients. Journal Clinical Oncology, 27, 3437-3444. doi: 10.1200/JCO.2008.18.9068.
Roberto, G. B., Ziliotto, L. & Seki, M. C. (2018). Avaliação comparativa de dosagem do marcador tumoral CA 15-3 em cadelas hígidas ou com neoplasia mamária. Pesquisa Veterinária Brasileira, 38(9), 1800-07. doi: 10.1590/1678-5150-PVB-5136
Sorenmo, K. U. (2011, outubro). Mammary gland tumors in cats: risk factors, clinical presentation, treatments and outcome. In Proceedings of the 36th World Small Animal Veterinary Congress. Jeju, Koreia.
Sousa, R. P. M. (2010). A expressão da COX-2 em patologias da glândula mamária da gata. (Dissertação de mestrado em Métodos Biomoleculares). Universidade de Aveiro, Aveiro, Portugal.
Togni, M., Masuda, E. K., Kommers, G. D., Fighera, R. A., Irigoyen, L. F. (2013). Estudo retrospectivo de 207 casos de tumores mamários em gatas. Pesquisa Veterinária Brasileira, 33(3), 353-58. Recuperado de https://www.scielo.br/j/pvb/a/YyvnGjfR3mS3gn7B5VZHz7P/?format=pdf&lang=pt
Zar, J. H. (1998). Biostatistical analysis. New Jersey, United States of America: Pearson.
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Copyright (c) 2022 Amanda de Deus Ferreira Alves; Márcia de Figueiredo Pereira ; José dos Passos Queiroz Júnior; Saulo Romero Félix Gonçalves; Ana Paula Veras Sobral ; Márcia Bersane Araújo de Medeiros Torres ; Valdemiro Amaro da Silva Junior
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