Development of electrochemical biosensor: Voltammetric analysis of lymphocytes and indication activation of complement system

Authors

DOI:

https://doi.org/10.33448/rsd-v11i7.30198

Keywords:

Lymphocytes; Biosensor; Blood cells.

Abstract

This work reports the development of an electrochemical biosensor after immobilization of the lymphocytes to detect the reaction between antibodies and specific HLA antigens present in the serum samples. A clean homemade gold electrode with voltammetric polycrystalline characteristics was used. Lymphocytes were immobilized and tested with positive and negative human serum and complements on the gold electrode. The experiments were carried out in a cell with three electrodes: working - gold, reference - Ag/AgCl/sat. KCl and auxiliary - platinum. The cyclic voltammetric analyses of immobilized lymphocytes on the gold surface presented anodic current equal to 1.78 μA at c.a. 0.50 V vs. Ag/AgCl/sat. KCl. The electrochemical responses of the serum (positive and negative) and complement do not show signs of oxidation or reduction in the potential range used. The electrodes with cells and positive serum showed the amplified current signal in the oxidation potential of the cells. The electrode was developed to verify the antigen antibody reaction, present lymphocyte cell and human serum samples. The electrode was qualitatively efficient when compared to the methods of flow cytometric analysis and complement dependent cytotoxicity, being able to be used with operational and economic advantages.

Author Biography

Ruan Sousa Bastos, Federal Institute of Education, Science and Technology of Maranhão; Research Group in Medicinal Chemistry and Biotechnology-QUIMEBIO

Graduado em Ciências Naturais/Química pela Universidade Federal do Maranhão - UFMA, Campus de Grajaú. Mestrando em Química Medicinal e Modelagem Molecular - UFPA. Participou do Programa Institucional de Bolsa de iniciação a Docência - PIBID e do Programa de Residência Pedagógica. Membro do Grupo de Pesquisa em Química Medicinal e Biotecnologia - QUIMEBIO e membro do Grupo de Pesquisa em Ciências Naturais e Biotecnologia - CIENATEC. Atua nas linhas de Biotecnologia e Química Quântica Computacional. E-mail: sonruanquimica@gmail.com

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Published

05/06/2022

How to Cite

OLIVEIRA, T. P.; MELO, S. M.; MONTE, S. J. H. do; BASTOS, R. S.; PASSOS, I. N. G.; SILVA, A. S.; SANTOS JÚNIOR, J. R. dos. Development of electrochemical biosensor: Voltammetric analysis of lymphocytes and indication activation of complement system. Research, Society and Development, [S. l.], v. 11, n. 7, p. e59611730198, 2022. DOI: 10.33448/rsd-v11i7.30198. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30198. Acesso em: 23 nov. 2024.

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Section

Exact and Earth Sciences