The efficiency of cleaning and disinfection of Dental school clinic surfaces: macroscopic and microscopic view
DOI:
https://doi.org/10.33448/rsd-v11i8.30770Keywords:
Bacteria; Contamination; Microbiology; School clinic; Dentistry.Abstract
In the dental clinic, it is common the dispersion of aerosols and droplets during the practice of procedures, through oral secretions, to the surfaces of benches and dental equipment. These biological secretions may contain pathogenic microorganisms that pose a risk of cross-contamination within the dental environment. Based on the risks and concern for the safety of professionals and patients, the objective of this research was to analyze the surfaces of the environment of a dental school clinic in the north of Tocantins, regarding dirt and the presence of microorganisms after use, cleaning and disinfection. For the analysis, the clinical care box was selected by drawing lots, and it was verified if there was dirt using visual inspection and microbiological contamination of the surfaces: reflector handle, triple syringe, sucker, spittoon, auxiliary table, sink faucet, and box partitions behind the operator and assistant. Analysis followed by microbiological growth in BHI and Nutrient Agar Culture, and by microscopic analysis. Among the surfaces evaluated, the presence of dirt was observed, such as handprints and solid residues. And, also, microbiological growth, with the presence of bacterial morphotypes related to the genera that reside mainly in the oral microbiota. Therefore, it is concluded that the clinical environment had contaminated surfaces even after cleaning and disinfection.
References
Adhikari, A., Kurella, S., Banerjee, P., & Mitra, A. (2017). Aerosolized bacteria and microbial activity in dental clinics during cleaning procedures. Journal of Aerosol Science, 114, 209-218.
Alharbi, A., Alharbi, S., &Alqaidi, S. (2020). Guidelines for dental careprovisionduringthe COVID-19 pandemic. The Saudi dental journal, 32(4), 181-186.
Chimenos-Küstner, E., Giovannoni, M. L., & Schemel-Suárez, M. (2017). Dysbiosis as a determinant factor of systemic and oral pathology: Importance of micorbiome. Medicina Clínica (English Edition), 149(7), 305-309.
De Oliveira, R. C. G. (2005). Sugestões de protocolo para o controle de infecção cruzada em Clínicas de Radiologia Odontológica. UningáJournal, 6(1).
de Sousa, K. S., & Fortuna, J. L. (2011). Microrganismos em ambientes climatizados de consultórios odontológicos em uma cidade do extremo sul da Bahia. Revista Baiana de Saúde Pública, 35(2), 250-250.
Ferreira, H. M. M. (2012). Utilização do método ATP bioluminescência na avaliação da eficácia da limpeza e desinfeção de superfícies em cuidados de saúde primários (Doctoraldissertation).
Franz, A., Bristela, M., &Stauffer, F. (2012). Reprocessingof dental instruments in washer-disinfectors: does a representativetestsoilexist in dentistry?. GMS Krankenhaushygieneinterdisziplinär, 7(1).
Kuhn, C. R., Toralles, R. P., Machado, M., Fanka, L. S., & Meireles, T. P. (2018). Contaminação microbiana em consultórios odontológicos. Revista brasileira de ciências da saúde, 22(4), 315-324.
Laheij, A. M., Kistler, J. O., Belibasakis, G. N., Välimaa, H., de Soet, J. J., & European Oral Microbiology Workshop (EOMW) 2011 (2012). Healthcare-associated viral and bacterial infections in dentistry. Journal of oral microbiology, 4, 10.3402/jom.v4i0.17659.
Macedo, M. M. L. P. (2017). Avaliação da higiene das superfícies em unidade de saúde com aplicação do luminómetro e da escala visual (Doctoraldissertation).
Peng, X., Xu, X., Li, Y., Cheng, L., Zhou, X., & Ren, B. (2020). Transmission routes of 2019-nCoV and controls in dental practice. International journal of oral science, 12(1), 1-6.
Pinelli, C., Garcia, P. P. N. S., Campos, J. Á. D. B., Dotta, E. A. V., & Rabello, A. P. (2011). Biossegurança e odontologia: crenças e atitudes de graduandos sobre o controle da infecção cruzada. Saúde e sociedade, 20, 448-461.
Rodrigues, K. A., Lima, I. F., da Silva, A. K., Sotte, D. M. K. S., & da Silva, J. S. (2019). Análise de contaminantes microbiológicos em consultório odontológico. Anais do Seminário Científico do UNIFACIG, (4).
Rossi, D., Devienne, K. F., &Raddi, M. S. G. (2008). Influência de fluídos biológicos na sobrevivência de Staphylococcus aureus sobre diferentes superfícies secas. Revista de Ciências Farmacêuticas Básica e Aplicada, 209-212.
Rutala, W. A., & Weber, D. J. (2005). The benefitsofsurfacedisinfection. American JournalofInfectionControl, 33(7), 434-435.
Sampaio-Maia, B., Caldas, I. M., Pereira, M. L., Pérez-Mongiovi, D., & Araujo, R. (2016). The Oral Microbiome in Health and Its Implication in Oral and Systemic Diseases. Advances in applied microbiology, 97, 171–210.
Sedky N. A. (2013). Occupational bloodborne exposure incident survey & management of exposure incidents in a dental teaching environment. International journal of health sciences, 7(2), 174–190.
Sun, J., Pan, L., Tsang, D. C., Zhan, Y., Zhu, L., & Li, X. (2018). Organiccontaminationandremediation in theagriculturalsoilsof China: A critical review. Science ofthe Total Environment, 615, 724-740.
Trabulsi, L. R.; & Alterthum, F. (2015). Microbiologia. Editora Alterthum. (6a ed.).
Volgenant, C. M. C., & De Soet, J. J. (2018). Cross-transmission in the dental office: does thismakeyouill?. Current oral healthreports, 5(4), 221-228.
Younes, T., Freddo, S. L., &Lucietto, D. A. (2017). Biossegurança em Odontologia: o ponto de vista dos pacientes. Arquivos em Odontologia, 53.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Mariana Fernandes de Sousa; Mikaela Alves da Silva; Iangla Araújo de Melo Damasceno; Ana Lúcia Roselino Ribeiro
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
