Growth and nutrition of Urochloa brizantha cv. Marandu inoculated with plant growth promoting bacteria and under different doses of nitrogen
DOI:
https://doi.org/10.33448/rsd-v9i10.8925Keywords:
B. brizantha; Biological nitrogen fixation; Diazotrophic bactéria; Environment; Sustainability.Abstract
The demand for the production of adequate amounts of plant biomass is a major challenge for Brazilian livestock that faces a serious situation in relation to the degradation of most native or cultivated pastures, especially in soils deficient in biogenic nutrients such as nitrogen. The objective of the present study was to evaluate the growth and nutrition of Urochloa brizantha as a result of inoculation with Azospirillum brasilense associated with nitrogen fertilization. The experiment was conducted in a greenhouse at Universidade Paranaense - UNIPAR, Umuarama - PR. The experimental design was completely randomized, consisting of six treatments (control, inoculated without N, inoculated + 50 and 75 kg ha-1 N, without inoculation + 50 and 75 kg ha-1 N) and five replicates each. 10 days after sowing, excess plants were thinned leaving only five plants per pot. 60 days after sowing, the plants were collected for dry mass analysis, leaf elongation and leaf nutrient content. The inoculation resulted in a gain in the dry mass of the aerial part, length of the aerial part and number of tillers, however there was no gain in dry mass of the root. It was possible to observe a higher concentration of leaf nitrogen and chlorophyll index in treatments with higher concentrations of nitrogen fertilization, inoculated or not. Therefore, it is concluded that inoculation with A. brasilense associated with nitrogen fertilization proved to be an excellent ally in increasing production and in the development of U. brizantha cv. Marandu.
References
Alexandrino, E., Vaz, R. G. M. V., & dos Santos, A. C. (2010). Características da Brachiaria brizantha cv. Marandu durante o seu estabelecimento submetida a diferentes doses de nitrogênio. Bioscience Journal, 26(6), 886-893.
Barbosa, R. A. (Ed.). (2006). Morte de pastos de braquiárias. Embrapa. p. 206.
Bezerra, J. D. do V., Emerenciano Neto, J. V., Alves, D. J. da S., Batista Neta, I. E., Galdino Neto, L. C., Santos, R. da S., & Difante, G. dos S. (2020). Características produtivas, morfogênicas e estruturais de cultivares de Brachiaria brizantha cultivadas em dois tipos de solo. Research, Society and Development, 9(7), e129972947.
Cabral, C. E. A., da Silva Cabral, L., Silva, E. M. B., dos Santos Carvalho, K., Kroth, B. E., & Cabral, C. H. A. (2016). Resposta da Brachiaria brizantha cv. Marandu a fertilizantes nitrogenados associados ao fosfato natural reativo. Comunicata Scientiae, 7(1), 66-72.
Chiari, L., Resende, R. M. S., Jank, L., do Valle, C. B., & Jungmann, L. (2007). A biotecnologia nos programas de melhoramento de forrageiras tropicais da Embrapa Gado de Corte. Embrapa Gado de Corte. p. 33.
Costa, K. A. D. P., Faquin, V., Oliveira, I. P. D., Rodrigues, C., & Severiano, E. D. C. (2008). Doses e fontes de nitrogênio em pastagem de capim-marandu: I-alterações nas características químicas do solo. Revista Brasileira de Ciência do Solo, 32(4), 1591-1599.
Dartora, J., Guimarães, V. F., Marini, D., & Sander, G. (2013). Adubação nitrogenada associada à inoculação com Azospirillum brasilense e Herbaspirillum seropedicae na cultura do milho. Revista Brasileira de Engenharia Agrícola e Ambiental, 17(10), 1023-1029.
Delevatti, L. M., Cardoso, A. S., Barbero, R. P., Leite, R. G., Romanzini, E. P., Ruggieri, A. C., & Reis, R. A. (2019). Effect of nitrogen application rate on yield, forage quality, and animal performance in a tropical pasture. Scientific reports, 9(1), 1-9.
Duarte, C. F. D., Cecato, U., Hungria, M., Fernandes, H. J., Biserra, T. T., Mamédio, D., Galbeiro, S., & Nogueira, M. A. (2020). Inoculação de bactérias promotoras do crescimento vegetal em Urochloa Ruziziensis. Research, Society and Development, 9(8), e630985978.
Falker Automação Agrícola. (2008). Manual do medidor eletrônico de teor clorofila (ClorofiLOG/CFL 1030).
Gitti, D. D. C., Arf, O., Portugal, J. R., Corsini, D. C. D. C., Rodrigues, R. A. F., & Kaneko, F. H. (2012). Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto. Bragantia, 71(4), 509-517.
Gobbi, K. F., Garcia, R., Garcez Neto, A. F., Pereira, O. G., Bernardino, F. S., & Rocha, F. C. (2005). Composição química e digestibilidade in vitro do feno de Brachiaria decumbens Stapf. tratado com uréia. Revista Brasileira de Zootecnia, 34(3), 720-725.
Leghari, S. J., Wahocho, N. A., Laghari, G. M., HafeezLaghari, A., MustafaBhabhan, G., HussainTalpur, K., ... & Lashari, A. A. (2016). Role of nitrogen for plant growth and development: A review. Advances in Environmental Biology, 10(9), 209-219.
Leite, R. D. C., dos Santos, J. G., Silva, E. L., Alves, C. R., Hungria, M., Leite, R. D. C., & dos Santos, A. C. (2019). Productivity increase, reduction of nitrogen fertiliser use and drought-stress mitigation by inoculation of Marandu grass (Urochloa brizantha) with Azospirillum brasilense. Crop and Pasture Science, 70(1), 61-67.
Li, H., Qiu, Y., Yao, T., Ma, Y., Zhang, H., & Yang, X. (2020). Effects of PGPR microbial inoculants on the growth and soil properties of Avena sativa, Medicago sativa, and Cucumis sativus seedlings. Soil and Tillage Research, 199, 104577.
Oleńska, E., Małek, W., Wójcik, M., Swiecicka, I., Thijs, S., & Vangronsveld, J. (2020). Beneficial features of plant growth-promoting rhizobacteria for improving plant growth and health in challenging conditions: A methodical review. Science of The Total Environment, e140682.
Oliveira, P. P. A., de Oliveira, W. S., & Barioni Junior, W. (2007). Produção de forragem e qualidade de Brachiaria brizantha cv. Marandu com Azospirillum brasilense e fertilizada com nitrogênio. Embrapa Pecuária Sudeste-Circular Técnica (INFOTECA-E).
Pietramale, R. T. R., Valentim, J. K., Marques, O. F. C., Leite, B. K. V., Petromali, G. F. S. G., Castilho, V. A. R. de, Barbosa, D. K., Ruviaro, C. F., Oliveira Neto, S. S. de, & Heinrichs, R. (2020). Marandu grass production under nutrient deficient conditions at different cutting times. Research, Society and Development, 9(4), e170943058.
Rampim, L., Guimarães, V. F., Salla, F. H., Costa, A. C. P. R., Inagaki, A. M., Bulegon, L. G., & França, R. (2020). Desenvolvimento inicial de plântulas de milho reinoculadas com bactérias diazotróficas. Research, Society and Development, 9(5), e24953109.
Reis, V. M., Baldani, V. L. D., & Baldani, J. I. (2005). Ecologia, isolamento e identificação de bactérias diazotróficas. In: Aquino, A. M.; Assis, R. L (Ed.). Processos biológicos no sistema solo-planta – ferramentas para uma agricultura sustentável. Brasília: Embrapa agrobiologia, 258-279 p.
Sambatti, J. A., Souza Junior, I. G., Costa, A. C. S., & Tormena, C. A. (2003). Estimativa da acidez potencial pelo método do pH SMP em solos da formação Caiuá – Noroeste do estado do Paraná. Revista Brasileira de Ciência do Solo, 27, 257-264.
Silva, F. C. D. S. (Ed.). (2009). Manual de análises químicas de solos, plantas e fertilizantes. Brasília: Embrapa Informação Tecnológica; Rio de Janeiro: Embrapa Solos. 243-627 p.
Silveira, A. P. D., Sala, V. M. R., Cardoso, E. J. B. N., Labanca, E. G., & Cipriano, M. A. P. (2016). Nitrogen metabolism and growth of wheat plant under diazotrophic endophytic bacteria inoculation. Applied Soil Ecology, 107, 313-319.
Simili, F. F., Barbosa, K. R. S., Augusto, J. G., Menegatto, L. S., Mendonça, G. G., Bonacim, P. M., ... & Savegnago, R. P. (2019). Study of the chemical composition of Urochloa brizantha using the SPAD index, neural networks, multiple linear models, principal components and cluster analysis. Animal Feed Science and Technology, 258, e114307.
Souza, E. L., da Cruz, P. J. R., Bonfá, C. S., & Magalhães, M. A. (2018). Plantas forrageiras para pastos de alta produtividade. Nutritime Revista Eletrônica, on-line, 15(4), 8271-8284.
Souza, P. T. D. (2014). Inoculação com Azospirilum brasilense e adubação nitrogenada em Brachiaria brizantha cv. Marandu. (Dissertação). Mestrado em Produção Vegetal. Universidade Federal de Goiás, Jataí, Goiás.
Souza. J. E. B., & Ferreira. E. P. B. (2017). Improving sustainability of common bean production systems by co-inoculating rhizobia and azospirilla. Agriculture, Ecosystems and Environment 237, 250–257.
Taiz, L., Zeiger, E., Møller, I. M., & Murphy, A. (2017). Fisiologia e desenvolvimento vegetal. Artmed Editora. 858 p.
Tropicos. (2020). Missouri botanical garden W3 tropicos. Vascular trópico. Disponível em:
http://www.tropicos.org/. Acesso em: 10. Set. 2020.
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