Análisis toxicológica y morfológica de Apis mellifera africanizada seleccionada por tolerancia al neonicotinoide tiametoxam

Autores/as

DOI:

https://doi.org/10.33448/rsd-v10i2.12109

Palabras clave:

Histología; Microscopía electrónica de barrido; Toxicología.

Resumen

Las abejas son el insecto más utilizado con fines de polinización por sus características eficientes para esta función, lo que se refleja en aspectos positivos tanto para la naturaleza como para el hombre. La expansión de la agricultura y el desarrollo de agroquímicos para combatir plagas ha tenido impactos negativos en la salud de las abejas, provocando su desaparición en todo el mundo. Esta investigación tuvo como objetivo evaluar los efectos de la exposición de las abejas melíferas al insecticida neonicotinoide tiametoxam sobre su tasa de supervivencia, así como sobre los cambios morfológicos e histológicos en el intestino medio de trabajadores adultos de la generación F4 de reinas Apis mellifera tolerantes al tiametoxam y trabajadoras no tolerantes abejas. Después de los bioensayos, se extrajo el intestino medio para evaluación morfológica. Los resultados mostraron que las abejas F4 eran más tolerantes al tiametoxam y exhibían cambios morfológicos menos significativos en comparación con el grupo de control no tolerante.

Biografía del autor/a

William Cristian da Silva Pizzaia, Universidade Estadual de Maringá

Biology degree at Faculdade Global de Umuarama (FGU). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). PhD in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Tamiris de Oliveira Diniz, Universidade Estadual de Maringá

Biology degree at Universidade Paranaense (Unipar). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). Specialist in Biotechnology at Universidade Estadual de Maringá (UEM). PhD student in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Breno Gabriel da Silva, Universidade Estadual de Maringá

Mathematics degree at Universidade Estadual de Maringá (UEM). Master in Biostatistics at Universidade Estadual de Maringá (UEM). PhD student in Statistics and Agricultural Experimentation at Escola Superior de Agricultura "Luiz de Queiroz" - Universidade de São Paulo (ESALQ / USP).

Naiara Climas Pereira, Universidade Estadual de Maringá

Biology degree at the Universidade Estadual de Maringá (UEM). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). PhD student in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Adriana Aparecida Sinópolis-Gigliolli, Universidade Estadual de Maringá

PhD in Biological Sciences at Universidade Estadual de Maringá (UEM). Adjunct professor at the Department of Biotechnology, Genetics and Cell Biology at Universidade Estadual de Maringá (UEM).

Angélica de Souza Khatlab, Universidade Estadual de Maringá

PhD in Zootechnics at Universidade Estadual de Maringá (UEM).

Eliane Gasparino, Universidade Estadual de Maringá

PhD in Zootechnics at Universidade Federal de Viçosa (UFV). Associate professor at the Department of Zootechnics at Universidade Estadual de Maringá (UEM).

Vagner de Alencar Arnaut de Toledo, Universidade Estadual de Maringá

PhD in Zootechnics at Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP). Full professor at the Zootechnics Department at Universidade Estadual de Maringá (UEM).

Maria Claudia Colla Ruvolo-Takasusuki, Universidade Estadual de Maringá

PhD in Genetics and Evolution at Universidade Federal de São Carlos (UFSCAR). Associate professor at the Department of Biotechnology, Genetics and Cell Biology at Universidade Estadual de Maringá (UEM).

Citas

Brown, M. J. & Paxton, R. J. (2009). The conservation of bee: a global perspective. Apidologie, v. 40, n. 3.

Bourgeois, A. L. et al. (2016). Molecular genetic analysis of Varroa destructor mites in brood, fallen injured mites, and worker bee longevity in honey bees. Journal of Apicultural Research, v. 54, n. 4.

Carreck, N. L. & Ratnieks, F. L. W. (2014). The dose makes the poison: have “field realistic” rates of exposure of bees to neonicotinoid insecticides been overestimated in laboratory studies? Journal of Apicultural Research, v. 53, n. 5.

Carvalho, S. M. et al. (2013). Enzymatic biomarkers as tools to assess environmental quality: a case study of exposure of the honey bee Apis mellifera to insecticides. Environmental Toxicology Chemistry, v. 32, n. 9.

Catae, A. F. et al. (2014). Cytotoxic Effects of Thiamethoxam in the Midgut and Malpighian Tubules of Africanized Apis mellifera (Hymenoptera: Apidae). Microscopy research and technique, v. 77, n. 4.

Catae, A. F. et al. (2018). Exposure to a sublethal concentration of imidacloprid and the side effects on target and nontarget organs of Apis mellifera (Apidae). Ecotoxicology, 27, n. 2.

Chambers, R. G. et al. (2019). Sub-lethal concentrations of neonicotinoid insecticides at the field level affect negatively honey yield: Evidence from a 6-year survey of Greek apiaries. PLOS ONE, v. 14, n. 4.

Cooper, D. M. et al. (2009). The insect caspases. Apoptosis, v. 14, n. 3.

Cresswell, J. E. & Thompson, H. (2012). Comment on “a common pesticide decreases foraging success and survival in honey bees”. Science, v. 337, n. 6101.

Cresswell, J. E. (2011). A meta-analisysof experiments testing the effects of a neonicotinoid insecticide on honeybees. Ecotoxicology, v. 20, n. 1.

Cruz, A. S. et al. (2010). Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae. Cell Biology and Toxicology, v. 26, n. 2.

Cunha, J. G. C. (2002). Melhoramento de abelhas e produção de rainhas. In: Anais do Congresso Nacional de Apicultura. Campo Grande: CBA.

Daberkow, S. & Phipps, R. (2015) Honey marketing for the commercial beekeeper. In: Graham, J. M. (eds.). The hive and the honey bee. Revised Edition. U.S.A.: Dadant and Sons, Hamilton, Illinois.

Diniz, T.O. et al. (2020) Toxicity and effects of combined agrochemical in Scaptotrigona bipunctata bees. Scientific Electronic Archives, v. 13, n. 12.

Falco, J. R. P. et al. (2010). Toxicity of thiamethoxam, behavioral effects and alterations in chromatin of Apis mellifera L, 1758 (Hymenoptera; Apidae). Research Journal Agriculture Biological Sciences, v. 6, n. 6.

Freitas, P. V. D. X. et al. (2016) Declínio populacional das abelhas polinizadoras: Revisão. Pubvet, v. 11, n. 1.

Gallai, N. et al. (2009) Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecological Economics, v. 68, n. 3.

Girolami, V. et al. (2009). Translocation of neonicotinoid insecticides from coated seeds to seedling guttation drops: a novel way of intoxication for bees. Journal of Economic Entomology, v. 102, n. 5.

Hassett, J. et al (2018). A significant pure population of the dark European honey bee (Apis mellifera mellifera) remains in Ireland. Journal of Apicultural Research, v. 57, n. 3.

Imperatriz-Fonseca, V. L. & Nunes-Silva, P. (2010). As abelhas, os serviços ecossistêmicos e o Código Florestal Brasileiro. Biota Neotropica, v. 10, n. 4.

Jamnik, P. et al. (2007). Antioxidative action of royal jelly in the yeast cell. Experimental Gerontology, v. 42, n. 7.

Junqueira, L. C. U & Junqueira, L. M. M. S. (1983). Basic Techniques of Cell Biology. São Paulo, Brazil: Santos.

Kerr, W. E. (1967) The history of introduction of African bees to Brazil. South African Bee Journal, v. 39, n. 1.

Kessler, S. C. et al. (2015). Bees prefer foods containing neonicotinoid pesticides. Nature, v. 10, n. 4.

Landim, C. C. (2009). Abelhas – Morfologia e função de sistemas. Ed. I. São Paulo: Editora UNESP, 408p.

Levy, S. M. et al. (2011). The role of peritrophic membrane in the resistance of Anticarsia gemmatalis larvae (Lepidoptera: Noctuidae) during the infection by its nucleopolyhedrovirus (AgMNPV). Arthropod Structure & Development, v. 40, n. 5.

Martinez, O. & Soares, A. E. E. (2012). Melhoramento genético na apicultura comercial para produção da própolis. Revista Brasileira de Saúde e Produção Animal, v. 13, n. 4.

McNeil, M. E. A. & Schmidt, J. O. (2015). Other products of the hive. In: Graham, J. M. (eds.). The hive and the honey bee. Revised Edition. U.S.A.: Dadant and Sons, Hamilton, Illinois.

Moussian, B. (2013). The apical plasma membrane of chitin-synthesizing epithelial. Insect Science, v. 20, n. 2.

Nakasu, E. Y. et al. (2014). Novel biopesticide based on a spider venom peptide shows no adverse effects on honey bees. Proceedings of the Royal Society of London B: Biological Sciences, v. 281, n. 1787.

Oliveira, A. C. et al. (2018). Pesticides affect pollinator abundance and productivity of sunflower (Helianthus annuus L.). Journal of Apicultural Research, v. 58, n. 1.

Oliveira, R. A. et al. (2014). Side‐effects of thiamethoxam on the brain and midgut of the africanized honeybee Apis mellifera (Hymenopptera: Apidae). Environmental Toxicology, v. 29, n. 10.

Pizzaia, W. C. S. et al. (2021) Apis mellifera africanized queens tolerant to the neonicotinoid thiamethoxam. Scientific Electronic Archives, v. 14, n. 2.

Queiroz, M. L. et al. (2001). Produção de Geléia Real e Desenvolvimento da Larva de Abelhas Apis mellifera, na Região Semi-Árida de Pernambuco. Revista Brasileira de Zootecnia, v. 30, n. 2.

Roat, T. C. et al. (2013). Effects of sublethaldose of fipronil on neuron metabolic activity of africanized honeybees. Archives of Environmental Comtamination and Toxicology, v. 64, n. 3.

Rossi, A. C. et al. (2013) Effects of sublethal doses of imidacloprid in malpighian tubules of africanized Apis mellifera (Hymenoptera, Apidae). Microscopy Research and Technique, v. 76, n. 5.

Sanchez-Bayo, F. et al. (2016). Are bee diseases linked to pesticides? A brief review. Environment International, v. 89, n. 1.

Seitz, N. et al. (2015). A national survey of managed honey bee 2014–2015 annual colony losses in the USA. Journal of Apicultural Research, v. 54, n. 4.

Serrão, J. E. & Landim, C. C. (2000). Ultrastructure of the midgut epithelium of Meliponinae larvae with different developmental stages and diets. Journal Apicultural, v. 39, n. 1.

Simone-Friston, M. & Spivak, M. (2010). Propolis and bee healt: the natural history and significance of resin use by honey bees. Apidologie, v. 41, n. 3.

Snodgrass, R. E. (1956). Anatomy and physiology of the honeybees. New York: Comstock Publishing Associates.

Stein, K. et al. (2017). Bee pollination increases yield quantity and quality of cash crops in Burkina Faso, West Africa. Scientific Reports, v. 7, n. 1.

Suchail, S. et al. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental Toxicology and Chemistry, v. 20, n. 11.

Suryanarayanan, S. & Kleinman, D. L. (2013). Be(e)coming experts: The controversy over insecticides in the honey bee colony collapse disorder. Social Studies of Science, v. 43, n. 2.

Tarpy, D. R. et al (2010). Mating frequencies of Africanized honey bees in the south western USA. Journal of Apicultural Research, v. 49, n. 4.

Tavares, D. A. et al. (2015). In vitro effects of thiamethoxam on larvae of Africanized honey bee Apis mellifera (Hymenoptera: Apidae). Chemosphere, v. 135, n. 7.

Teixeira, A. D. et al. (2013). Degeneration and cell regeneration in the midgut of Podisus nigrispinus (Heteroptera: Pentatomidae) during post-embryonic development. Arthropod Structure Development, v. 42, n. 3.

Terra, W. R. (2001). The origin and functions of the insect peritrophic membrane and peritrophic gel. Archives Insect Biochemistry Physiology, v. 47, n. 2.

Toledo, V. A. A. & Mouro, G. F. (2005). Produção de geleia real com abelhas Africanizadas selecionadas e cárnicas híbrida. Revista Brasileira Zootecnia, v. 34, n. 6.

Williamson, S. M. et al. (2014). Exposure to neonicotinoids influences the motor function of adult worker honeybees. Ecotoxicology, v. 23, n. 8.

Zakour, M. K., & Bienefeld, K. (2015). Basic considerations in the development of breeding plans for honey bees, illustrated by data on the native Syrian honey bee (Apis mellifera syriaca). Journal of Apicultural Research, v. 53, n. 2.

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Publicado

08/02/2021

Cómo citar

PIZZAIA, W. C. da S.; DINIZ, T. de O.; SILVA, B. G. da; PEREIRA, N. C.; SINÓPOLIS-GIGLIOLLI, A. A. .; KHATLAB, A. de S. .; GASPARINO, E.; TOLEDO, V. de A. A. de .; RUVOLO-TAKASUSUKI, M. C. C. . Análisis toxicológica y morfológica de Apis mellifera africanizada seleccionada por tolerancia al neonicotinoide tiametoxam . Research, Society and Development, [S. l.], v. 10, n. 2, p. e14310212109, 2021. DOI: 10.33448/rsd-v10i2.12109. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12109. Acesso em: 28 sep. 2024.

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Sección

Ciencias Agrarias y Biológicas