Effect of a whitlockite glass-ceramic on the occlusion of dentinal tubules for dentin hypersensitivity treatment
DOI:
https://doi.org/10.33448/rsd-v10i3.13161Keywords:
Biomaterials; Bioactive glass-ceramic; Dentin remineralization; 3CaO.P2O5-SiO2-MgO-system; Dentin hypersensitivity.Abstract
A hipersensibilidade dentinária (HD) é caracterizada pela dor curta e aguda em resposta a estímulos externos, podendo ser tratada pela oclusão dos túbulos dentinários. Neste estudo, vidro original e uma vitrocerâmica do sistema 3CaO.P2O5-SiO2-MgO contendo whitlockita (fase de fosfato tricálcico substituído com Mg) foram avaliados quanto à eficácia no tratamento da HD. Um vidro bioativo com composição 52,75.(3CaO.P2O5)–30SiO2–17,25MgO (%p) foi tratado termicamente a 700°C e 775°C por 4 h. Terceiros molares humanos foram desmineralizados e distribuídos aleatoriamente em grupos: NT - controle negativo (sem tratamento), BG - controle positivo (tratado com Bioglass®45S5), GL - tratamento com vidro original e WGC - tratamento com whitlockita. Em seguida, amostras de dentina foram imersas em saliva artificial e armazenadas por 7 dias a 37ºC. Os biomateriais foram avaliados por Difração de Raios X (XRD) e Microscopia Eletrônica de Varredura (MEV). As amostras de dentina foram analisadas por MEV e ATR-FTIR, para identificar e quantificar variações composicionais na superfície dentinária. Foi identificada whitlockita como fase cristalina do GC, e sua presença leva à formação de partículas arredondadas, conforme mostrado por imagens de MEV. A análise de ATR-FTIR confirmou a formação de apatita na superfície de amostras BG, GL e WGC, o que foi corroborado pela oclusão dos túbulos dentinários, conforme imagens em MEV. As intensidades dos picos de infravermelho foram comparadas estatisticamente. Em relação à reatividade superficial pela análise semiquantitativa de FTIR, o GC demonstrou similaridade com o Bioglass®45S5 indicando que este material é promissor no tratamento de HD.
References
Addy, M. (2002). Dentine hypersensitivity: new perspectives on an old problem. International Dental Journal, 52, 367-375.
Ana, P. A., Tabchoury, C. P., Cury, J. A., & Zezell, D. M. (2012). Effect of Er,Cr:YSGG laser and professional fluoride application on enamel demineralization and on fluoride retention. Caries Research, 46(5), 441-51.
Apel, E., Deubener, J., Bernard, A., Holand, M., Muller, R., Kappert, H., Rheinberger, V., & Holland W. (2008). Phenomena and mechanisms of crack propagation in glass-ceramics. Journal of Mechanical Behavior of Biomedical Material, 1(4), 313-325.
Bakry, A .S., Sadr, A., Takahashi, H., Otsuki, M., & Tagami, J. (2007). Analysis of Er:YAG Laser Dentin using Attenuated Total Reflectance Fourie Transform Infrared and X-ray Diffaction Techniques. Dental Material Journal, 26(3), 422-428.
Bakry, A .S., Takahashi, H., & Otsuki, M. (2011). CO2 Laser Improves 45S5 Bioglass Interaction with Dentin. Journal of Dental Research, 90(2), 246-250.
Bamise, C. T., & Esan, T. A. (2011). Mechanisms and treatment approaches of dentine hypersensitivity: a literature review. Oral Health Preventive Dentistry, 9(4), 353-67.
Blatz, M.B. (2012). Laser Therapy may be Better Than Topical Desensitizing Agents for Treating Dentin Hypersensitivity. Journal of Evid-Based Dental Practice, 12(2), 69-70.
Chatzistavrou, X., Rao, R. R., & Caldwell, D. J. (2016). Collagen/fibrin microbeads as a delivery system for Ag-doped bioactive glass and DPSCs for potential applications in dentistry. Journal of Non-Crystalline Solids, 432(15A), 143-149.
Crovace, M. C., Souza, M. T., & Chinaglia, C. R. (2016). Biosilicate® - A multipurpose, highly bioactive glass-ceramic. In vitro, in vivo and clinical trials. Journal of Non-Crystalline Solids, 432, 90-110.
Cunha, S. R., Garófalo, S.A., Scaramucci, T., Zezell, D. M., & Aranha, A. C. C. (2017). The association between Nd:YAG laser and desensitizing dentifrices for the treatment of dentin hypersensitivity. Lasers Medicine Science, 32, 873-880.
Daguano, J. K. M. F., Rogero, S. O., Crovace, M. C., Peitl, O., Strecker, K., & Santos, C. (2013). Bioactivity and Cytotoxicity of Glass and Glass Ceramics Based on the 3CaO.P2O5–SiO2-MgO System. Journal of Materials Science: Materials in Medicine, 24, 2171-2180.
Daguano, J. K. M. F., Strecker, K., Ziemath, E. C., Rogero, S. O., Fernandes, M. H. V., & Santos, C. (2012). Effect of partial crystallization on the mechanical properties and cytotoxicity of bioactive glass from the 3CaO.P2O5-SiO2-MgO system. Journal of Mechanical Behavior of Biomedical Material, 14, 78-88.
Farooq, I., Moheet, I. A., & AlShwaimi, E. (2015). In vitro dentin tubule occlusion and remineralization competence of various toothpastes. Archives of Oral Biology, 60, 1246-1253.
Fornaini, C., Brulat-Bouchard, N., Medioni, E., Zhang, S., Rocca, J., Merigo, E. (2020) Nd:YAP laser in the treatment of dentinal hypersensitivity: An ex vivo study. Journal of Photochemistry and Photobiology B: Biology, 203, 111740.
Gallob, J., Ling, M. R., Amini, P., Patil, A., Atassi, M. (2019). Efficacy of a dissolvable strip with calcium sodium phosphosilicate (NovaMin®) in providing rapid dentine hypersensitivity relief. Journal of Dentistry: X, 2, 100003.
Gondim, A. L. M. F., Barbosa, G. A. S., Dantas, W. R. M., Dantas, E. M., Oliveira, H. T. R. de ., Almeida Neto, L. F. de, Marcelino, K. P., & Pagnoncelli, R. M. . (2020). Effect of laser therapy on osteogenesis in skullcap defects filled with α-TCP cement and β-TCP/HA granules: animal model. Research, Society and Development, 9(10), e6889109061.
Hench, L.L., & Jones, J.R. (2015). Bioactive Glasses: Frontiers and Challenges. Frontiers in Bioengineering Biotechnology, 3, 194.
Huang, M., Hill, R.G., & Rawlinson, S.C.F. (2017). Zinc bioglasses regulate mineralization in human dental pulp stem cells. Dental Materials, 33(5), 543-552.
Kim, J., & Park, J. Dentin hypersensitivity and emerging concepts for treatments. (2017). Journal of Oral Bioscience, 59, 211-217.
Kulal, R., Jayanti, I., Sambashivaiah, S., & Bilchodmath, S. (2016). An In-vitro Comparison of Nano Hydroxyapatite, Novamin and Proargin Desensitizing Toothpastes - A SEM Study. Journal of Clinical and Diagnostic Research, 10(10), ZC51-ZC54.
Ma, Q., Wang, T., Meng, Q., Xu, X., Wu, H., Xu, D., & Chen, Y. (2017). Comparison of in vitro dentinal tubule occluding efficacy of two different methods using a nano-scaled bioactive glass-containing desensitising agent. Journal of Dentistry, 60, 63–69.
Mantzourania, M., & Sharma, D. (2013). Dentine sensitivity: Past, present and future Journal of Dentistry, 41(4), s3-s17.
Miguez-Pacheco, V., Hench, L.L., & Boccaccini, A.R. (2015). Bioactive glasses beyond bone and teeth: Emerging applications in contact with soft tissues. Acta Biomaterialia, 13, 1-15.
Mitchell, J.C., Musanje, L., & Ferracane, J.L. (2011). Biomimetic dentin desensitizer based on nano-structured bioactive glass. Dental Material, 27, 386-393.
Montazerian, M., & Zannoto E.D. (2016). History and trends of bioactive glass-ceramics. Journal of Biomedical Material Research Part A, 104, 1231-1249.
Moreira, M. M., Silva, L. R. R., Mendes, T. A. D, Santiago, S. L., Mazzetto, S. E., Lomonaco, D., & Feitosa, V. P. (2018). Synthesis and characterization of a new methacrylate monomer derived from the cashew nutshell liquid (CNSL) and its effect on dentinal tubular occlusion. Dental Material, 34, 1144-1153.
Nascimento, M. E. S., Júnior, J. R. L. S., Lima, M. V. A., Almeida, N. M. S., Hora, S.L., & Cabral, L.L. (2020). Etiology and treatment of dentin hyperesensitivity today: integrative review. Research, Society and Development, 9(8), e661986192.
Peitl, O., LaTorre, G. P., & Hench. L.L. (1996). Effect of crystallization on apatite-layer formation of bioactive glass 45S5. Journal of Biomedical Material Research, 30(4), 509-14.
Peitl, O., Zanotto, E. D., Serbena, F. C., & Hench, L. L. (2012). Compositional and microstructural design of highly bioactive P2O5-Na2O-CaO-SiO2 glass-ceramics. Acta Biomaterialia, 8, 321-332.
Seong, J., Newcombe, R. G., Matheson, J. R., Weddell, L., Edwards, M., & West, N. X. (2020). A randomised controlled trial investigating efficacy of a novel toothpaste containing calcium silicate and sodium phosphate in dentine hypersensitivity pain reduction compared to a fluoride control toothpaste. Journal of Dentistry, 98, 103320.
Shiau, H. J. (2012). Dentin Hypersensitivity. Journal of Evid-Based Dental Practice, 12(3), 220-28.
Song, J., Wang, H., Yang, Y., Xiao, Z., Lin, H., Jin, L., Lin, M., Chen, F., Zhu, M., Zhao, Y., Qiu, Z., Li, Y., & Zhang, X. (2018). Nanogels of carboxymethyl chitosan and lysozyme encapsulated amorphous calcium phosphate to occlude dentinal tubules. Journal of Materials Science: Materials in Medicine, 29, 84-95.
Suge, T., Kawasaki, A., Ishikawa, K., Matsuo, T., & Ebisu, S. (2008). Ammonium hexafluorosilicate elicits calcium phosphate precipitation and shows continuous dentin tubule occlusion. Dental Material, 24(2), 192-198.
Tartari, T., Bachmann, L., Zancan, R. F., Vivian, R. R., Duarte, M. A. H., & Bramante, C. M. (2018). Analysis of the effects of several decalcifying agents alone and in combination with sodium hypochlorite on the chemical composition of dentine. International Endodhotic Journal, 51(1), e42-e54.
Thanatvarakorn, O., Nakashima, S., Sadr, A., Ikeda, M., & Tagami, J. (2013). In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium–phosphate desensitizer Journal of Biomedical Material Research Part B. 101, 303-309.
Tirapelli, C., Panzeri, H., Lara, E. H., Soares, R. G., Peitl, O., & Zanotto, E. D. (2011). The effect of a novel crystallised bioactive glass-ceramic powder on dentine hypersensitivity: a long-term clinical study. Journal of Oral Rehabilitation, 38(4), 253-62.
Tirapelli, C., Panzeri, H., Soares, R. G., Peitl, O., & Zannoto, E. D. (2010). A novel bioactive glass-ceramic for treating dentin hypersensitivity. Brazilian Oral Research, 24, 381-387.
Tunar, O. L., Gursoy, H., Çakar, G., Kuru, B., Ipci, S. D., & Yilmaz, S. (2014). Evaluation of the Effects of Er:YAG Laser and Desensitizing Paste Containing 8% Arginine and Calcium Carbonate, and Their Combinations on Human Dentine Tubules: A Scanning Electron Microscopic Analysis. Photomedicine and Laser Surgery, 32(10), 540-545.
Wang, S., Gao, X., Gong, W., Zhang, Z., Chen, X., & Dong, Y. (2014). Odontogenic differentiation and dentin formation of dental pulp cells under nanobioactive glass induction. Acta Biomaterialia., 10, 2792-2803.
Zhong, Y., Liu, J., Li, X., Yin, W., He, T., Hu, D., Liao, Y., Yao, X., & Wang, Y. (2015). Effect of a novel bioactive glass-ceramic on dentinal tubule occlusion: an in vitro study. Australian Dental Journal, 60, 96–103.
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Copyright (c) 2021 Amanda de Castro Juraski; Daniela Casimiro Figueredo; Nasser Ali Daghastanli; Claudinei dos Santos; Maria Helena Vaz Fernandes; Patrícia Aparecida da Ana; Juliana Kelmy Macário Barboza Daguano
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