Actividad de nisina contra Staphylococcus aureus resistente y sensible a meticilina y riesgo de adquisición de resistência
DOI:
https://doi.org/10.33448/rsd-v10i7.16178Palabras clave:
Resistencia a antibióticos; Actividad antimicrobiana; Bacteriocina; Staphylococcus aureus.Resumen
El objetivo de este estudio fue analizar el efecto antimicrobiano de la nisina contra MRSA y MSSA (Staphylococcus aureus resistente a meticilina y sensible a meticilina), y al mismo tiempo examinar la posibilidad de que la bacteria desarrolle resistencia a la nisina. La susceptibilidad antimicrobiana de las cepas se evaluó mediante los métodos de difusión en agar y / o microdilución. Para seleccionar cepas resistentes a la nisina, se cultivaron bacterias consecutivamente a concentraciones subletales de la bacteriocina. La nisina mostró actividad bactericida contra la mayoría de las cepas probadas. MRSA requirió dosis más altas de bacteriocina en comparación con MSSA tanto para la inhibición como para la muerte celular. Sin embargo, las transferencias en presencia de nisina podrían eliminar completamente la actividad de la nisina con un aumento en el valor de MIC de hasta 250 veces. La resistencia a la nisina podría mantenerse en MRSA y MSSA incluso en ausencia de la bacteriocina. La resistencia a la nisina afectó la susceptibilidad antibiótica de MRSA y MSSA principalmente a cefoxitina, oxacilina y eritromicina. Estos resultados indican que la resistencia a la nisina es un rasgo complejo entre MSSA y MRSA y debe aclararse antes de la recomendación terapéutica de la nisina para tratar infecciones causadas por estas especies bacterianas.
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Derechos de autor 2021 César Matos Ribeiro da Silva; Waleska da Silva Albuquerque ; Jucyara Natállia Araujo de Oliveira ; Maria Regina Pires Carneiro ; Sona Jain ; Silvio Santana Dolabella ; Ana Andréa Teixeira Barbosa
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