Actividades antioxidante, antimicrobiana y citotóxica de los metabolitos secundarios de Streptomyces sp. aislado de la región Amazónica-Brasileña
DOI:
https://doi.org/10.33448/rsd-v10i10.18974Palabras clave:
Actinobacteria; Anticancer; Compuestos fenólicos; Citotoxicidad.Resumen
Las bacterias del género Streptomyces son una fuente prometedora de productos biológicamente activos, con aplicaciones en medicina, industria y agricultura. Por lo tanto, el objetivo de este trabajo fue evaluar las actividades citotóxicas, antioxidantes y antimicrobianas de los extractos y fracciones semipurificadas de Streptomyces spp. aislada de la rizósfera de Paullinia cupana, Amazonas-Brasil. Para ello, se realizó un estudio bioguiado de la actividad citotóxica con extracto metanólico de Streptomyces sp. El extracto ACTMS-12H UFPEDA 3405 (EMeOH-12H) fue fraccionado con n-hexano, acetato de etilo y 2-butanol. La actividad antioxidante fue analizada a través de los métodos de DPPH, ABTS y fosfomolibdeno, mientras que la actividad antimicrobiana fue investigada por el método de microdilución para determinar la concentración minima inhibitoria (MIC) contra especies de bacterias y levaduras. En la prueba de citotoxicidad la fase butanólica (FbuOH-12H) presentó una IC50 de 1.1 µg/mL contra MOLT-4, con muerte celular probablemente por apoptosis, pero no causó citotoxicidad en las células mononucleares de sangre periférica (PBMC) o en eritrocitos humanos, La prospección química detectó la presencia de saponinas y azúcares reductores en la fracción de 2-butanol (FBuOH-12H), que pueden estar relacionados con la citotoxicidad. Sobre la actividad antioxidante por ABTS, la fracción de acetato de etilo (FAcOEt-12H) mostró una capacidad antioxidante de 1161.7 ± 0.04 µM de Trolox/g de extracto, indicando una expresiva reactividad de la fase con este radical. Las fases acuosas (de los extractos de hexano, acetato de etilo y metanol) fueron activas en todos los microorganismos probados, excepto E. faecalis.
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Derechos de autor 2021 Sandrine Maria Arruda Lima; Pedro Silvino Pereira; Bruno Iraquitan Miranda da Silva; Natalie Emanuelle Ribeiro; Elizabeth Fernanda de Oliveira Borba; Cícera Datiane de Morais Oliveira Tintino; Karina Perrelli Randau; Henrique Douglas Melo Coutinho; Gláucia Manoella de Souza Lima-Gomes; Mónica Lizeth Chávez-González; Glória Alicia Martinez-Medina; Maria das Graças Carneiro-da- Cunha; Teresinha Gonçalves Silva
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