Evaluación de la inmunotinción de Fibronectina y Tenascina inducida por la reparación de cementos biocerámicos: un estudio en tejido subcutáneo de ratas wistar
DOI:
https://doi.org/10.33448/rsd-v10i10.19325Palabras clave:
Endodontia; Imuno-Histoquímica; Inflamação; Teste de Materiais; Tenascina.Resumen
El objetivo del estudio fue evaluar la presencia de inmunomarcadores de fibronectina y tenascina en el tejido subcutáneo de ratas con cemento reparador biocerámico Biodentine®, en comparación con MTA Blanco Angelus® y Ca(OH)2. Se implantaron tubos de polietileno en el tejido subcutáneo de 32 ratas Wistar macho que contenían los materiales y un tubo vacío para el control (n = 8 animales). Después de los días 7, 15, 30 y 60, se sacrificó a los animales, se retiraron los tubos de polietileno con los tejidos circundantes y se prepararon las muestras para el análisis de inmunotinción. Los datos se analizaron mediante la prueba de Kruskal-Wallis y Dunn con un nivel de significancia del 5%. Los materiales mostraron un patrón moderado de inmunotinción para fibronectina después de 7 días y tenascina después de 15 días. El grupo Biodentine a los 60 días fue el único que mostró un alto nivel de inmunotinción para las glicoproteínas. Podemos concluir que el cemento Biodentine indujo un mayor patrón de inmunotinción de tenascina y fribronectina en 60 días, igualando MTA Blanco Angelus® y Ca(OH)2 en los otros períodos, mostrando resultados superiores a otros materiales.
Citas
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Derechos de autor 2021 Diego Valentim; Carlos Roberto Emerenciano Bueno; Marina Tolomei Sandoval Cury; Ana Maria Veiga Vasques; Ana Claudia Rodrigues da Silva ; Francine Benetti; Gustavo Sivieri Araujo; Rogerio Castilho Jacinto; João Eduardo Gomes-Filho; Edilson Ervolino; Luciano Tavares Angelo Cintra; Eloi Dezan-Junior
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