Estudio comparativo de la composición química, actividades larvicidas, antimicrobianas y citotóxicas de aceites volátiles de hojas de E. punicifolia de Minas Gerais y Goiás
DOI:
https://doi.org/10.33448/rsd-v10i11.19354Palabras clave:
Aceite essencial; Quimodiversidad; Planta medicinal; Actividades biológicas; Myrtaceae.Resumen
Eugenia punicifolia (Kunth) D.C., Myrtaceae, conocida como “pedra-ume-caá”, se usa popularmente en el tratamiento de inflamación, infecciones, fiebre, gripe, diabetes y diarrea. El objetivo de este trabajo fue realizar un estudio comparativo de la composición química del aceite volátil de hojas de E. punicifolia recolectadas en Goiás y Minas Gerais, así como evaluar la actividad larvicida frente a larvas de Aedes aegypti L3, la actividad antimicrobiana frente a bacterias, hongos patógenos y ambientales, y actividad citotóxica para las células Balb 3T3 (fibroblastos murinos). Los aceites volátiles se obtuvieron por hidrodestilación en un aparato Clevenger y se analizaron mediante cromatografía de gases acoplada a espectrometría de masas (CG MS). Se identificaron un total de 60 compuestos, siendo los principales componentes encontrados en las hojas de Goiás Germacrene D, biciclogermacreno y β-longipenene y en las hojas recolectadas en Minas Gerais fueron (Z) -cariofileno, γ-cadineno, spathulenol, óxido de cariofileno. y α-cadinol. El efecto larvicida fue moderado contra Ae. aegypti, con CL50 de 85,53 µg / mL para muestras de Goiás y CL50 de 91,52 µg/mL para muestras de Minas Gerais. Ambos aceites mostraron un potencial bactericida moderado contra K. rhiziphyla (ATCC 9341), M. luteus (ATCC 10240) y S. aureus (ATCC 29737). En cuanto a la citotoxicidad, los aceites de Goiás (IC50 706,7 µg / mL) y Minas Gerais (IC50 160,7 µg / mL) presentaron una concentración citotóxica menor que la acción tóxica para larvas y bacterias, evidenciando un perfil de seguridad y un interesante potencial terapéutico, principalmente con respecto al aceite volátil de Goiás. Por lo tanto, los aceites volátiles de las hojas de E. punicifolia recolectadas en Goiás y Minas Gerais presentaron un potencial larvicida moderado para Ae. aegypti, actividad bactericida y menor citotoxicidad frente a fibroblastos murinos. Este es el primer estudio de la actividad larvicida, antimicrobiana y citotóxica de los aceites volátiles de las hojas de E. punicifolia.
Citas
Adams, R.P. (2007). Identification of essential oil components by Gas Chromatography/Mass Spectrometry. (4th ed.), Carol Stream, Illinois: Allured Publishing Corporation.
Alam, P., Ansari, M. J., Anwer, M. K., Raish, M., Kamal, Y. K. T., & Shakeel, F. (2016). Wound healing effects of nanoemulsion containing clove essential oil. Artificial Cells, Nanomedicine, and Biotechnology, 45(3), 591–597. https://doi.org/10.3109/21691401.2016.1163716
An, N. T. G., Huong, L. T., Satyal, P., Tai, T. A., Dai, D. N., Hung, N. H., Ngoc, N. T. B., & Setzer, W. N. (2020). Mosquito larvicidal activity, antimicrobial activity, and chemical compositions of essential oils from four species of myrtaceae from Central Vietnam. Plants, 9(4), 544. https://doi.org/10.3390/plants9040544
Andrade, P. M., Melo, D. C., Alcoba, A. E. T., Ferreira júnior, W. G., Pagotti, M. C., Magalhães, L. G., Santos, T. C. L. Dos, Crotti, A. E. M., Alves, C. C. F., & Miranda, M. L. D. (2018). Chemical composition and evaluation of antileishmanial and cytotoxic activities of the essential oil from leaves of Cryptocarya aschersoniana Mez. (Lauraceae Juss.). Anais Da Academia Brasileira de Ciências, 90(3), 2671–2678. https://doi.org/10.1590/0001-3765201820170332
Araújo, I. S. (2011). Atividade antimicrobiana de plantas aromáticas que ocorrem no Estado do Pará. Dissertação de Mestrado, Programa de Pós-graduação em Biotecnologia, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia.
Barros, F. M. C., Zambarda, E. O., Heinzmann, B. M., & Mallmann, C. A. (2009). Variabilidade sazonal e biossíntese de terpenóides presentes no óleo essencial de Lippia alba (Mill.) N. E. Brown (Verbenaceae). Química Nova, 32(4), 861–867. https://doi.org/10.1590/S0100-40422009000400007
Basting, R. T., Nishijima, C. M., Lopes, J.., Santos, R. C., Lucena Périco, L., Laufer, S., Bauer, S., Costa, M. F., Santos, L. C., Rocha, L. R. M., Vilegas, W., Santos, A. R. S., Dos Santos, C., & Hiruma-Lima, C. (2014). Antinociceptive, anti-inflammatory and gastroprotective effects of a hydroalcoholic extract from the leaves of Eugenia punicifolia (Kunth) DC. in rodents. Journal of Ethnopharmacology, 157, 257–267. https://doi.org/10.1016/j.jep.2014.09.041
Benelli, G., Govindarajan, M., AlSalhi, M. S., Devanesan, S., & Maggi, F. (2018). High toxicity of camphene and γ-elemene from Wedelia prostrata essential oil against larvae of Spodoptera litura (Lepidoptera: Noctuidae). Environmental Science and Pollution Research, 25(11), 10383–10391. https://doi.org/10.1007/s11356-017-9490-7
Benelli, G., Govindarajan, M., Rajeswary, M., Vaseeharan, B., Alyahya, S. A., Alharbi, N. S., Kadaikunnan, S., Khaled, J. M., & Maggi, F. (2018). Insecticidal activity of camphene, zerumbone and α-humulene from Cheilocostus speciosus rhizome essential oil against the Old-World bollworm. Helicoverpa armigera. Ecotoxicology and Environmental Safety, 148, 781–786. https://doi.org/10.1016/j.ecoenv.2017.11.044
Bezerra Filho, C. M., Silva, L. C. N., Silva, M. V., Løbner-Olesen, A., Struve, C., Krogfelt, K. A., Correia, M. T. S., & Vilela Oliva, M. L. (2020). Antimicrobial and antivirulence action of Eugenia brejoensis essential oil in vitro and in vivo invertebrate models. Frontiers in Microbiology, 11, 1–11. https://doi.org/10.3389/fmicb.2020.00424
Bilia, A. R., Guccione, C., Isacchi, B., Righeschi, C., Firenzuoli, F., & Bergonzi, M. C. (2014). Essential oils loaded in nanosystems: A developing strategy for a successful therapeutic approach. Evidence-Based Complementary and Alternative Medicine, 2014, 1–14. https://doi.org/10.1155/2014/651593
Chau, D. T. M., Chung, N. T., Huong, L. T., Hung, N. H., Ogunwande, I. A., Dai, D. N., & Setzer, W. N. (2020). Chemical compositions, mosquito larvicidal and antimicrobial activities of leaf essential oils of eleven species of lauraceae from Vietnam. Plants, 9(5), 606. https://doi.org/10.3390/plants9050606
Costa, A. A., Naspi, C. V., Lucia, A., & Masuh, H. M. (2017). Repellent and larvicidal activity of the essential oil from Eucalyptus nitens against Aedes aegypti and Aedes albopictus (Diptera: Culicidae). Journal of Medical Entomology, 54(3), 670–676. https://doi.org/10.1093/jme/tjw222
Coneglian, I.R.M. (2007). Morfologia e ontogênese do pericarpo e semente de Eugenia punicifolia (H.B. e K.) DC., Myrcia bela Camb. e Campomanesia pubescens (DC.) Berg (Myrtaceae). Dissertação de mestrado Universidade Estadual de Campinas, Campinas.
Costa, J. S., Cruz, E. de N. S., Setzer, W. N., da Silva, J. K. D. R., Maia, J. G. S., & Figueiredo, P. L. B. (2020). Essentials Oils from Brazilian Eugenia and Syzygium Species and Their Biological Activities. Biomolecules, 10(8), 1155. https://doi.org/10.3390/biom10081155
Cruz, E. M. de O., Pinto, J. A. O., Fontes, S. S., Arrigoni-Blank, M. de F., Bacci, L., Jesus, H. C. R. de, Santos, D. de A., Alves, P. B., & Blank, A. F. (2014). Water deficit and seasonality study on essential oil constituents of Lippia gracilis Schauer Germplasm. The Scientific World Journal, 2014, 1–9. https://doi.org/10.1155/2014/314626
Dahham, S., Tabana, Y., Iqbal, M., Ahamed, M., Ezzat, M., Majid, A., & Majid, A. (2015). The Anticancer, Antioxidant and Antimicrobial Properties of the Sesquiterpene β-Caryophyllene from the Essential Oil of Aquilaria crassna. Molecules, 20(7), 11808–11829. https://doi.org/10.3390/molecules200711808
Oliveira, R. N., Dias, I. J. M., & Camara, C. A. G. (2005). Estudo comparativo do oleo essencial de Eugenia punicifolia (HBK) DC. de diferentes localidades de Pernambuco. Rev.Brasileira de Farmacognosia, 15(1), 39–43. https://doi.org/10.1590/S0102-695X2005000100009
Dias, C. N. (2013). Avaliação da atividade larvicida em Aedes aegypti L. (Diptera: Culicidae) de óleos essenciais de espécies vegetais: um estudo de revisão e bioprospecção. Dissertação de mestrado, Programa de Pós-Graduação em Saúde e Ambiente da Universidade Federal do Maranhão, São Luís.
Dias, C. N., Alves, Luciana, Patricia, L., Antonio, K., Rodrigues, F., Brito, Maria, C. A., Rosa, C. D. S., Amaral, F. M. M. D., Monteiro, O. D. S., Andrade, E. H. D. A., Maia, J. G. S., & Moraes, D. F. C. (2015). Chemical composition and larvicidal activity of essential oils extracted from Brazilian Legal Amazon plants against Aedes aegypti L. (Diptera : Culicidae). Evidence-Based Complementary and Alternative Medicine, 2015, 8. https://doi.org/10.1155/2015/490765
Van Den Dool, H., & Kratz, P.D., 1963. A generalization of the retention index system in- cluding linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography A, 11, 463–471. https://doi.org/10.1016/S0021-9673(01)80947-X
Fernandes, C. C., Rezende, J. L., Silva, E. A. J., Silva, F. G., Stenico, L., Crotti, A. E. M., Esperandim, V. R., Santiago, M. B., Martins, C. H. G., & Miranda, M. L. D. (2021a). Chemical composition and biological activities of essential oil from flowers of Psidium guajava (Myrtaceae). Brazilian Journal of Biology, 81(3), 728–736. https://doi.org/10.1590/1519-6984.230533
Fernandes, Y., Matos, J., Lima, C., Tardini, A., Viera, F., Maia, J., Monteiro, O., Longato, G., & Rocha, C. (2021b). Essential oils obtained from aerial Eugenia punicifolia Parts: chemical composition and antiproliferative potential evidenced through cell cycle arrest. Journal of the Brazilian Chemical Society, 32(7), 1–10. https://doi.org/10.21577/0103-5053.20210036
Figueiredo, P. L. B., Pinto, L. C., da Costa, J. S., da Silva, A. R. C., Mourão, R. H. V., Montenegro, R. C., da Silva, J. K. R., & Maia, J. G. S. (2019). Composition, antioxidant capacity and cytotoxic activity of Eugenia uniflora L. chemotype-oils from the Amazon. Journal of Ethnopharmacology, 232, 30–38. https://doi.org/10.1016/j.jep.2018.12.011
Franco, C. de J. P., Ferreira, O. O., Morais, A. B., Â., Varela, E. L. P., Nascimento, L. D., Percário, S., de Oliveira, M. S., & Andrade, E. H. A. (2021). Chemical composition and antioxidant activity of essential oils from Eugenia patrisii Vahl, E. punicifolia (Kunth) DC., and Myrcia tomentosa (Aubl.) DC., leaf of family Myrtaceae. Molecules, 26(11), 3292. https://doi.org/10.3390/molecules26113292
Galeno, D. M. L., Carvalho, R. P., De Araújo Boleti, A. P., Lima, A. S., De Almeida, P. D. O., Pacheco, C. C., De Souza, T. P., & Lima, E. S. (2014). Extract from Eugenia punicifolia is an antioxidant and inhibits enzymes related to metabolic syndrome. Applied Biochemistry and Biotechnology, 172(1), 311–324. https://doi.org/10.1007/s12010-013-0520-8
Gobbo-Neto, L., & Lopes, N. P. (2007). Plantas medicinais: Fatores de influência no conteúdo de metabólitos secundários. Quimica Nova, 30(2), 374–381. https://doi.org/10.1590/S0100-40422007000200026
Govindarajan, M. (2010). Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (Willd.) Hook. f. ex Benth (Rutaceae) against three mosquito species. Asian Pacific Journal of Tropical Medicine, 3(11), 874–877. https://doi.org/10.1016/S1995-7645(10)60210-6
Govindarajan, M., & Benelli, G. (2016). α-Humulene and β-elemene from Syzygium zeylanicum (Myrtaceae) essential oil: highly effective and eco-friendly larvicides against Anopheles subpictus, Aedes albopictus, and Culex tritaeniorhynchus (Diptera: Culicidae). Parasitology Research, 115(7), 2771–2778. https://doi.org/10.1007/s00436-016-5025-2
Govindarajan, M., Rajeswary, M., & Benelli, G. (2016). δ-Cadinene,Calarene and δ-4-Carene from Kadsura heteroclita Essential Oil as Novel Larvicides Against Malaria, Dengue and Filariasis Mosquitoes. Combinatorial Chemistry & High Throughput Screening, 19(7), 565–571. https://doi.org/10.2174/1386207319666160506123520
Holetz, F. B., Pessini, G. L., Sanches, N. R., Cortez, A. G., Nakamura, C. V., & Filho, B. P. D. (2002). Screening of some plants used in the brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz, 97(October), 1027–1031.
ICCVAM (2006c), ICCVAM Test method evaluation report: in vitro cytotoxicity test methods for estimating starting doses for acute oral systemic toxicity tests. Research Triangle Park, NC: National Institute for Environmental Health Sciences. Available: [http://iccvam.niehs.nih.gov/methods/acutetox/inv_nru_tmer.htm
Komalamisra, N., Trongtokit, Y., Rongsriyam, Y., & Apiwathnasorn, C. (2005). Screening for larvicidal activity in some Thai plants against four mosquito vector species. Southeast Asian Journal of Tropical Medicine and Public Health, 36(6), 1412–1422.
Leite, P. E. C., Almeida, K. B., Lagrota-Candido, J., Trindade, P., Silva, R. F., Ribeiro, M. G. L., Lima-Araújo, K. G., Santos, W. C., & Quirico-Santos, T. (2010). Anti-inflammatory activity of Eugenia punicifolia extract on muscular lesion of mdx dystrophic mice. Journal of Cellular Biochemistry, 111(6), 1652–1660. https://doi.org/10.1002/jcb.22906
Lemos, V. O. T., Lucena, E. M. P. , Bonilla, O. H., & Edson-Chaves, B. (2019). Ecological anatomy of Eugenia punicifolia (Kunth) DC. (Myrtaceae) in the restinga region,state of Ceará. Revista Brasileira de Fruticultura, 41(6), 1–11. https://doi.org/10.1590/0100-29452019503
Lima, J. P. S. , Pinheiro, M. L. B., Santos, A. M. G., Pereira, J. L. S., Santos, D. M. F., Barison, A., Silva-Jardim, I., & Costa, E. V. (2012). In Vitro atileishmanial and cytotoxic activities of Annona mucosa (Annonaceae). Revista Virtual de Química, 4(6), 692–702. https://doi.org/10.5935/1984-6835.20120052
Lima, M. A. A., Oliveira, F. F. M., Gomes, G. A., Lovor, P. L., Santiago, G. M. P., Nagao-dias, A. T., Arriaga, Â. M. C., Lemos, T. L. G., & Carvalho, M. G. (2011). Evaluation of larvicidal activity of the essential oils of plants species from Brazil against Aedes aegypti ( Diptera : Culicidae ). African Journal of Biotechnology, 10(55), 11716–11720. https://doi.org/10.5897/AJB11.1102
Magina, M. D. A., Dalmarco, E. M., Wisniewski, A., Simionatto, E. L., Dalmarco, J. B., Pizzolatti, M. G., & Brighente, I. M. C. (2009). Chemical composition and antibacterial activity of essential oils of Eugenia species. Journal of Natural Medicines, 63(3), 345–350. https://doi.org/10.1007/s11418-009-0329-5
Maia, J. G. S., Zoghbi, M. G. B., & Luz, A. I. R. (1997). Essential oil of Eugenia punicifolia (HBK) DC. Journal of Essential Oil Research, 9(3), 337–338. https://doi.org/10.1080/10412905.1997.10554254
Martins, J. E. C. (1989). Plantas medicinais de uso na Amazônia. Ed. Cultural- CEJUP, (2nd ed.), 88-89.
Mendes, L. A., Martins, G. F., Valbon, W. R., da Silva de Souza, T., Menini, L., Ferreira, A., & da Silva Ferreira, M. F. (2017). Larvicidal effect of essential oils from Brazilian cultivars of guava on Aedes aegypti L. Industrial Crops and Products, 108, 684–689. https://doi.org/10.1016/j.indcrop.2017.07.034
Mora, F. D., Avila, J. L., Rojas, L. B., Ramírez, R., Usubillaga, A., Segnini, S., Carmona, J., & Silva, B. (2010). Chemical composition and larvicidal activity of Eugenia triquetra essential oil from Venezuelan Andes. Natural Product Communications, 5(6), 965–968. https://doi.org/10.1177/1934578x1000500633
Neves, I. A., Rezende, S. R. da F., Kirk, J. M., Pontes, E. G., & Carvalho, M. G. de. (2017). Composition and larvicidal activity of essential oil of Eugenia candolleana DC. (Myrtaceae) against Aedes aegypti. Revista Virtual de Química, 9(6), 2305–2315. https://doi.org/10.21577/1984-6835.20170138
Nogueira Sobrinho, A. C., Morais, S. M., Marinho, M. M., de Souza, N. V., & Lima, D. M. (2021). Antiviral activity on the Zika virus and larvicidal activity on the Aedes spp. of Lippia alba essential oil and β-caryophyllene. Industrial Crops and Products, 162, 113281. https://doi.org/10.1016/j.indcrop.2021.113281
OECD. (2010). Test Guideline No. 129: Guidance document on using cytotoxicity test to estimate starting doses for acute oral systemic toxicity tests. OECD Guidelines for the Testing Chemicals, July (129), 1–54.
Pascual, R. de, Colmena, I., Rios, C. de los, Rosa, J. M., Correa-Leite, P. E., Lima-Araújo, K. G., Ferreira, V. F., Rocha, D. R., Gonzaga, D. T. G., García, A. G., Santos, W. C., & Gandía, L. (2012). Augmentation of catecholamine release elicited by an Eugenia punicifolia extract in chromaffin cells. Revista Brasileira de Farmacognosia, 22(1), 1–12. https://doi.org/10.1590/S0102-695X2011005000191
Pereira, R. A., Zoghbi, M. das G. B., & Bastos, M. de N. do C. (2010). Essential Oils of Twelve Species of Myrtaceae Growing Wild in the Sandbank of the Resex Maracanã, State of Pará, Brazil. Journal of Essential Oil Bearing Plants, 13(4), 440–450. https://doi.org/10.1080/0972060X.2010.10643847
Ramos, M. F. D. S., Monteiro, S. D. S., da Silva, V. P., Nakamura, M. J., & Siani, A. C. (2010). Essential oils from Myrtaceae species of the Brazilian southeastern maritime forest (Restinga). Journal of Essential Oil Research, 22(2), 109–113. https://doi.org/10.1080/10412905.2010.9700275
Sarma, R., Adhikari, K., Mahanta, S., & Khanikor, B. (2019). Combinations of plant essential oil based terpene compounds as larvicidal and adulticidal agent against Aedes aegypti (Diptera: Culicidae). Scientific Reports, 9(1), 9471. https://doi.org/10.1038/s41598-019-45908-3
Senra, T. V. (2012). Ocorrência da broca (Coleoptera: curculionidae) em frutos de Eugenia punicifolia (kunth) dc. (Pedra-umecaá) em uma área experimental da EMBRAPA Amazônia Ocidental. 2012. 43 f. Dissertação (Mestrado em Agronomia Tropicall) - Universidade Federal do Amazonas, Manaus.
Silva, A. G., Alves, R. C. C., Filho, C. M. B., Bezerra-Silva, P. C., Santos, L. M. M. , Foglio, M. A., Navarro, D. M. A. F., Silva, M. V., & Correia, M. T. S. (2015). Chemical composition and larvicidal activity of the essential oil from leaves of Eugenia brejoensis Mazine (Myrtaceae). Journal of Essential Oil Bearing Plants, 18(6), 1441–1447. https://doi.org/10.1080/0972060X.2014.1000390
Silva, E. A. J. da, Silva, V. P. da, Alves, C. C. F., Alves, J. M., Souchie, E. L., & Barbosa, L. C. de A. (2016). Harvest time on the content and chemical composition of essential oil from leaves of guava. Ciência Rural, 46(10), 1771–1776. https://doi.org/10.1590/0103-8478cr20150947
Silva, I. G., Silva, H. H. G, Lima, C. G. (2003). Ovipositional behavior of Aedes aegypti (Diptera, Culicidae) in different strata and biological cycle. Acta Biológica Paranaense, 32, 1-8.
Silva, M. V. D. S. G. (2018). Composição química e atividade larvicida contra Aedes aegypti do óleo essencial da Eugenia calycina. Dissertação de mestrado - Universidade Federal de Uberlândia, Programa de Pós-Graduação em Química. Disponível em: http://dx.doi.org/10.14393/ufu.di.2018.1118
Silva, F. D. S., Landell, M. F., Paulino, G. V. B., Coutinho, H. D. M., & Albuquerque, U. P. (2020). Antifungal activity of selected plant extracts based on an ethnodirected study. Acta Botanica Brasilica, 34(2), 442–448. https://doi.org/10.1590/0102-33062020abb0003
Silva, J., Andrade, E., Barreto, L., da Silva, N., Ribeiro, A., Montenegro, R., & Maia, J. (2017). Chemical composition of four essential oils of Eugenia from the Brazilian Amazon and their cytotoxic and antioxidant activity. Medicines, 4(3), 51. https://doi.org/10.3390/medicines4030051
Silva, M. V., Silva, S. A., Teixera, T. L., De Oliveira, A., Morais, S. A. L., Da Silva, C. V., Espindola, L. S., & Sousa, R. M. F. (2021). Essential oil from leaves of Eugenia calycina Cambes: Natural larvicidal against Aedes aegypti. Journal of the Science of Food and Agriculture, 101(3), 1202–1208. https://doi.org/10.1002/jsfa.10732
Sousa, R. M. F., de Morais, S. A. L., Vieira, R. B. K., Napolitano, D. R., Guzman, V. B., Moraes, T. S., Cunha, L. C. S., Martins, C. H. G., Chang, R., de Aquino, F. J. T., do Nascimento, E. A., & de Oliveira, A. (2015). Chemical composition, cytotoxic, and antibacterial activity of the essential oil from Eugenia calycina Cambess. leaves against oral bacteria. Industrial Crops and Products, 65, 71–78. https://doi.org/10.1016/j.indcrop.2014.11.050
Steele, C. L., Crock, J., Bohlmann, J., & Croteau, R. (1998). Sesquiterpene synthases from grand fir (Abies grandis). Journal of Biological Chemistry, 273(4), 2078–2089. https://doi.org/10.1074/jbc.273.4.2078
Stefanello, M. É. A., Cervi, A. C., Ito, I. Y., Marcos, J., Jr, A. W., Simionatto, E. L., Élida, M., Cervi, A. C., Ito, I. Y., Marcos, J., Jr, A. W., Simionatto, E. L., Composition, C., Stefanello, M. É. A., Cervi, A. C., & Salvador, M. J. (2011). Chemical composition and antimicrobial activity of essential oils of Eugenia chlorophylla chemical composition and antimicrobial activity of essential oils of Eugenia chlorophylla ( Myrtaceae ). Journal of Essential Oil Research, 2905(20:1), 75–78. https://doi.org/10.1080/10412905.2008.9699427
Sobral, M., Proença, C., Souza, M., Mazine, F., Lucas, E. (2015). Myrtaceae in Lista de Espécies da Flora do Brasil. Jardim Botânico do Rio de Janeiro. Disponível em: <http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB10515>.
Toledo, A. G., Souza, J. G.L., Silva, J. P. B., Favreto, W. A. J., Costa, W. F., & Pinto, F. G. S. (2020). Chemical composition, antimicrobial and antioxidant activity of the essential oil of leaves of Eugenia involucrata DC. Bioscience Journal, 36(2), 568–577. https://doi.org/10.14393/BJ-v36n2a2020-48096
Turek, C., & Stintzing, F. C. (2013). Stability of essential oils: A Review. Comprehensive Reviews in Food Science and Food Safety, 12(1), 40–53. https://doi.org/10.1111/1541-4337.12006
Verma, R. S., Padalia, R. C., & Chauhan, A. (2014). Essential oil composition of Aegle marmelos (l.) Correa: Chemotypic and seasonal variations. Journal of the Science of Food and Agriculture, 94(9), 1904–1913. https://doi.org/10.1002/jsfa.6510
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2021 Liliane de Sousa Silva; Christiane França Martins; Fernando Yano Abrão; Camila Aline Romano; Soraia Ferreira Bezerra; Leonardo Luiz Borges; Adelair Helena dos Santos ; Luiz Carlos da Cunha; Jerônimo R. Oliveira Neto ; Tatiana de Sousa Fiuza ; José Realino de Paula
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Los autores que publican en esta revista concuerdan con los siguientes términos:
1) Los autores mantienen los derechos de autor y conceden a la revista el derecho de primera publicación, con el trabajo simultáneamente licenciado bajo la Licencia Creative Commons Attribution que permite el compartir el trabajo con reconocimiento de la autoría y publicación inicial en esta revista.
2) Los autores tienen autorización para asumir contratos adicionales por separado, para distribución no exclusiva de la versión del trabajo publicada en esta revista (por ejemplo, publicar en repositorio institucional o como capítulo de libro), con reconocimiento de autoría y publicación inicial en esta revista.
3) Los autores tienen permiso y son estimulados a publicar y distribuir su trabajo en línea (por ejemplo, en repositorios institucionales o en su página personal) a cualquier punto antes o durante el proceso editorial, ya que esto puede generar cambios productivos, así como aumentar el impacto y la cita del trabajo publicado.