Efecto del pH y la temperatura sobre la producción de fitasa y biomasa por fermentación sumergida con Aspergillus niger var. Phoenicis URM 4924
DOI:
https://doi.org/10.33448/rsd-v11i6.28994Palabras clave:
Aspergillus niger; Ergosterol; Fitasa; Fermentación sumergida.Resumen
La producción de fitasa y biomasa (estimada por el contenido de ergosterol) por fermentación sumergida con Aspergillus niger var. phoenicis URM 4924 fue estudiado. Se realizaron ensayos experimentales en diferentes condiciones de pH (4,0 a 8,0) y temperatura (25 a 35 ºC), y se estudió la influencia de estas variables en las respuestas mediante un diseño estadístico compuesto y metodología de superficie de respuesta. La producción de fitasa y biomasa se vio afectada por el pH y la temperatura utilizados durante la fermentación. La actividad de fitasa aumentó hasta 7,8 veces (de 1,04 a 8,09 U/ml) y la concentración de ergosterol aumentó hasta 38 veces (de 9,3 a 354,09 µg/ml). Los valores máximos de ambas respuestas se alcanzaron a pH 4,0 y 30 ºC. Se encontró una buena correlación (ajuste de segundo orden, R2 = 0,9875) entre los datos obtenidos para la actividad de fitasa y el contenido de ergosterol, lo que sugiere que la producción de fitasa depende de la formación de biomasa. Estos resultados son interesantes, ya que contribuyen al desarrollo de un proceso industrial para la producción de fitasa con altos rendimientos por fermentación sumergida.
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Derechos de autor 2022 Júlio Cézar dos Santos Nascimento; Apolônio Gomes Ribeiro; Ricardo Alexandre Silva Pessoa; Carlos Bôa Viagem Rabello; Armando Venâncio; Tatiana Souza Porto; José António Couto Teixeira; Ana Lúcia Figueiredo Porto
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