Producción ecológica de biosurfactante termoestable, halotolerante y amplio rango de pH por Issatchenkia orientalis UCP 1603

Autores/as

DOI:

https://doi.org/10.33448/rsd-v11i10.32851

Palabras clave:

Issatchenkia orientalis; Tensioactivo microbiano; Sustratos renovables; Estabilidad térmica; Tolerancia a alta salinidad.

Resumen

Los tensioactivos microbianos son moléculas anfifílicas con atractivas perspectivas industriales debido a sus ventajas sobre los tensioactivos químicos comúnmente comercializados, tales como como baja toxicidad, alta biocompatibilidad y biodegradabilidad, y eficiencia en condiciones extremas. Sin embargo, la producción a gran escala de biosurfactantes aún se vuelve poco competitiva debido al bajo rendimiento y los costos onerosos, considerándose el uso de sustratos renovables como una estrategia viable. En ese sentido, el presente estudio tuvo como objetivo investigar la producción sostenible de biosurfactante por la levadura Issatchenkia orientalis UCP 1603 en 2 L de medio a base de sales suplementado con agua residual de yuca 7,5%, licor de maíz macerado 5% y aceite de soya post fritura 1%. La fermentación se llevó a cabo en matraces Fernbach de 2,8 L durante 72 h, a 28°C y 150 rpm, con una reducción de la tensión superficial de 30,1 mN/m. El mayor rendimiento del biosurfactante producido (4,02 g/L) se observó después de la extracción con etanol al 70 % (2:1, v/v) y el biosurfactante aislado redujo la tensión superficial del medio a 28,7 mN/m, la tensión interfacial frente al n-hexadecano a 16,6 mN/m y tiene una CMC de 800 mg/L. El biocompuesto mostró naturaleza aniónica y polimérica, y no presentó toxicidad contra semillas de col (Brassica oleracea var. capitata). La estabilidad en el rango de pH 4-10, salinidad 5-25% y temperatura 5-100°C, evidenciaron un biosurfactante altamente estable, con prometedor potencial de aplicación en diversas actividades industriales o procesos ambientales en condiciones adversas.

Biografía del autor/a

Tainã Crisia de Souza Fonseca , Universidad Federal Rural de Pernambuco

Doctorado - RENORBIO

Dayana Montero Rodriguez, Universidad Católica de Pernambuco

Universidad Católica de Pernambuco

NPCIMB

 

Rafael de Souza Mendonça, Universidad Católica de Pernambuco

NPCIAMB-UNICAP

Isabela Natália da Silva Ferreira, Universidad Federal Rural de Pernambuco

Doctorado RENORBIO

Luiz Oliveira Costa, Universidad Católica de Pernambuco

NPCIAMB-UNICAP

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Publicado

04/08/2022

Cómo citar

FONSECA , T. C. de S. .; RODRIGUEZ, D. M.; MENDONÇA, R. de S. .; FERREIRA, I. N. da S. .; COSTA, L. O. .; CAMPOS-TAKAKI, G. M. Producción ecológica de biosurfactante termoestable, halotolerante y amplio rango de pH por Issatchenkia orientalis UCP 1603. Research, Society and Development, [S. l.], v. 11, n. 10, p. e364111032851, 2022. DOI: 10.33448/rsd-v11i10.32851. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/32851. Acesso em: 22 nov. 2024.

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Sección

Ingenierías