Osteogênese e formação de biofilmes em superfícies de titânio submetidas à implantação de íons por imersão em plasma de oxigênio
DOI:
https://doi.org/10.33448/rsd-v10i6.15644Palavras-chave:
Osteogênese; Biocompatibilidade; O-PIII; Biofilme; Liga de titânio.Resumo
Os objetivos deste estudo foram caracterizar superfícies de titânio (Ti) tratadas por implantação de íons por imersão em plasma de oxigênio (O-PIII) em distintas temperaturas, correlacionando tais camadas implantadas com efeitos terapêuticos e com osteogênese, bem como a formação de biofilmes monotípicos microbianos. Os grupos foram divididos em: a) Ti (pré-tratamento) b) Ti O-PIII a 400 ° C. c) Ti O-PIII a 500 ° C. d) Ti O-PIII a 600 ° C. As propriedades e características superficiais foram avaliadas de acordo com a rugosidade, textura, resistência à corrosão, formação de novas fases e a identificação de compostos químicos presentes. As análises celulares investigaram a interação celular, viabilidade, conteúdo de proteína total, fosfatase alcalina e quantificação de nódulos mineralizados usando células MG-63. A formação de biofilmes microbianos monotípicos, incluindo P. aeruginosa, S. aureus, S. mutans e C. albicans foram avaliadas. O aumento da rugosidade superficial, da resistência à corrosão e do teor de oxigênio, levando à formação de TiO2-rutilo com picos mais intensos e em maior número de acordo com o aumento da temperatura do substrato amostras de Ti implantadas iônicas foi observado. Houve também aumento significativo na viabilidade celular, produção de proteína total, atividade da fosfatase alcalina e formação de nódulos de mineralização para o grupo tratado com O-PIII a 600ºC em comparação com outros grupos, além de redução de microrganismos nos grupos tratados com O-PIII. Portanto, o tratamento com O-PIII a 600ºC em Ti grau IV apresentou resultados favoráveis para sua utilização.
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